Karina M M Carneiro

Karina M M Carneiro
University of Toronto | U of T · Faculty of Dentistry

BSc, PhD

About

38
Publications
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1,032
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Publications

Publications (38)
Article
Tissue fibrosis manifests as excessive deposition of compacted, highly aligned collagen fibrils, which interfere with organ structure and function. Cells in collagen‐rich lesions often exhibit marked overexpression of discoidin domain receptor 1 (DDR1), which is linked to increased collagen compaction through the association of DDR1 with the Ca2+‐d...
Article
Full-text available
Biomineralization is a crucial process whereby organisms produce mineralized tissues such as teeth for mastication, bones for support, and shells for protection. Mineralized tissues are composed of hierarchically organized hydroxyapatite crystals, with a limited capacity to regenerate when demineralized or damaged past a critical size. Thus, the de...
Article
Without evidence for an organic framework, biological and biochemical processes observed during amelogenesis provided limited information on how extracellular matrix proteins control the development of the complex fibrous architecture of human enamel. Over a decade ago, amelogenin nanoribbons were first observed from recombinant proteins during in...
Article
Strategies to improve hydroxyapatite mineralization for enamel repair are essential for tissue regeneration. Amelogenin and amelotin (AMTN) are enamel matrix proteins playing critical roles in enamel formation. Amelogenin acts as a scaffold for hydroxyapatite, while AMTN (specifically its ‘SSEEL’ domain) is necessary for proper enamel mineralizatio...
Article
Full-text available
Nature uses extracellular matrix scaffolds to organize biominerals into hierarchical structures over various length scales. This has inspired the design of biomimetic mineralization scaffolds, with DNA nanostructures being among the most promising. DNA nanotechnology makes use of molecular recognition to controllably give 1D, 2D and 3D nanostructur...
Article
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The formation of extensions in cell migration requires tightly coordinated reorganization of all three cytoskeletal polymers but the mechanisms by which intermediate filament networks interact with actin to generate extensions are not well-defined. We examined interactions of the actin binding protein filamin A (FLNA) with vimentin in extension for...
Article
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The field of DNA nanotechnology uses synthetic DNA strands as building blocks for designing complex shapes in one-, two- and three-dimensions. Here, we investigate whether DNA nanostructures are feasible platforms for the precise organization of polyaspartic acid (pAsp), a known mineral carrier, with a goal towards biomimetic mineralization for ena...
Article
Full-text available
Mineralized and sound dentin matrices contain inactive preforms of proteolytic enzymes that may be activated during the demineralization cycle. In this study, we tested the hypothesis that protease inhibitors (PI) preserve demineralized collagen fibrils and other constituents of the dentin matrix and thereby affect the potential for remineralizatio...
Article
Full-text available
Enamel, the outermost layer of teeth, is an acellular mineralized tissue that cannot regenerate; the mature tissue is composed of high aspect ratio apatite nanocrystals organized into rods and inter-rod regions. Amelogenin constitutes 90% of the protein matrix in developing enamel and plays a central role in guiding the hierarchical organization of...
Conference Paper
Heterogeneity in material systems requires methods for nanoscale chemical identification. Scattering scanning near-field microscopy (s-SNOM) is chemically sensitive in the infrared fingerprint region while providing down to 10 nm spatial resolution. This technique detects material specific tip-scattering in an atomic force microscope. Here, we pres...
Article
Full-text available
Dentinogenesis imperfecta type II (DGI-II) lacks intrafibrillar mineral with severe compromise of dentin mechanical properties. A Dspp knockout (Dspp–/–) mouse, with a phenotype similar to that of human DGI-II, was used to determine if poly-L-aspartic acid [poly(ASP)] in the “polymer-induced liquid-precursor” (PILP) system can restore its mechanica...
Conference Paper
Infrared spectroscopy can give valuable information on chemical composition, but far-field techniques such as FTIR spectroscopy are limited in spatial resolution. S-SNOM is a well-established near-field technique [1] that can overcome this diffraction limit, allowing an improvement in spatial resolution down to 10 nm. Our s-SNOM instrument is base...
Article
Full-text available
div class="title">New Hybrid Peak-Force Tapping/Near-Field Microscope for Nano-Chemical and Nano-Mechanical Imaging of Graphene Plasmons, Polymers and Proteins - Volume 21 Issue S3 - M. Wagner, G. Andreev, K. Carneiro, S. Habelitz, T. Mueller
Conference Paper
Full-text available
Objectives: Dentinogenesis imperfecta type II (DI-II), a heritable disease caused by mutations in the dentin sialophosphoprotein (Dspp) gene, is characterized by lack of intrafibrillar mineral with severe compromise of the mechanical properties of dentin. In this study, we used a Dspp knock-out (Dspp-KO) mouse model that displays a phenotype simila...
Article
Over the past 30 years DNA has been assembled into a plethora of structures by design, based on its reliable base pairing properties. As a result, many applications of DNA nanotechnology are emerging. Here, we review recent advances in the use of self-assembled DNA nanostructures as sensors. In particular, we focus on how defined nanostructures, su...
Article
Polymer self-assembly and DNA nanotechnology have both proved to be powerful nanoscale techniques. To date, most attempts to merge the fields have been limited to placing linear DNA segments within a polydisperse block copolymer. Here we show that by using hydrophobic polymers of a precisely pre-determined length conjugated to DNA strands, and addr...
Article
Full-text available
Here, we demonstrate a new approach for the design and assembly of a dynamic DNA cube with an addressable cellular uptake profile. This cube can be selectively unzipped from a 3D to a flat two-dimensional structure in the presence of a specific nucleic acid sequence. Selective opening is demonstrated in vitro using a synthetic RNA marker unique to...
Article
Inspired by biological polymers, sequence-controlled synthetic polymers are highly promising materials that integrate the robustness of synthetic systems with the information-derived activity of biological counterparts. Polymer-biopolymer conjugates are often targeted to achieve this union; however, their synthesis remains challenging. We report a...
Article
Sequence-controlled polymers attached to DNA can be prepared by solid-phase phosphoramidite coupling, as H. F. Sleiman, C. J. Serpell, and co-workers report in their Communication on page 4567 ff. Polymers with the same molecular composition but different monomer patterns exhibit different amphiphilic self-assembly. As the DNA component still retai...
Article
Nature uses a combination of non-covalent interactions to create a hierarchy of complex systems from simple building blocks. One example is the selective association of the hydrophobic side chains that are a strong determinant of protein organization. Here, we report a parallel mode of assembly in DNA nanotechnology. Dendritic alkyl-DNA conjugates...
Article
Long-range assembly of DNA currently comprises both top-down and bottom-up methods. The top-down techniques consist of physical alignment of DNA and lithographic patterning to organize DNA on surfaces. The bottom-up approaches include lipid-and polymer–DNA co-assembly, the self-assembly of DNA amphiphiles, and the remarkably specific and versatile...
Article
Full-text available
DNA nanotubes have great potential as nanoscale scaffolds for the organization of materials, the templation of nanowires, and as drug delivery vehicles. Current methods for making DNA nanotubes either rely on a tile-based step-growth polymerization mechanism, or use a large number of component strands and long annealing times. Step-growth polymeriz...
Article
The selective association of hydrophobic sidechains is a strong determinant of protein organization. We have observed a parallel mode of assembly in DNA nanotechnology. Firstly, dendritic DNA amphiphiles (D-DNA) were synthesized (Carneiro, Aldaye, & Sleiman, 2009) comprising an addressable oligonucleotide portion and a hydrophobic alkyl dendron at...
Article
Full-text available
DNA nanotubes are an attractive class of materials that can be assembled with precise control over their size, shape, length and porosity, and can encapsulate and release materials in response to specific added molecules. In parallel, block copolymer assemblies can provide biocompatibility, stability, nuclease resistance, the ability to encapsulate...
Chapter
DNA has emerged as a powerful building block for the construction of supramolecular materials, because of its molecular recognition specificity, programmability through sequence selection, ease of functionalization, an array of enzyme-assisted modifications, and automated synthesis. This molecule has been used to create a variety of constructs, ran...
Article
Full-text available
Here, we introduce a 3D-DNA construction method that assembles a minimum number of DNA strands in quantitative yield, to give a scaffold with a large number of single-stranded arms. This DNA frame is used as a core structure to organize other functional materials in 3D as the shell. We use the ring-opening metathesis polymerization (ROMP) to genera...
Article
DNA nanotubes hold promise as scaffolds for protein organization, as templates of nanowires and photonic systems, and as drug delivery vehicles. We present a new DNA-economic strategy for the construction of DNA nanotubes with a backbone produced by rolling circle amplification (RCA), which results in increased stability and templated length. These...
Article
Trimethylsilyl methylenenitronate reacts with both aliphatic and aromatic aldehydes in the presence of catalytic amounts of scandium(III) triflate to form the Henry reaction products in low (25%) to good yields (77%). This is the first example of this silyl nitronate undergoing a nitro-aldol reaction under an acid environment.
Article
A simple method to introduce the long-range order achieved by block copolymers into DNA structures is described. This results in the hierarchical assembly of short DNA strands into a new one-dimensional material, with high aspect ratio and the ability to further align into highly ordered surfaces over tens of micrometers. Fibers derived from biolog...
Article
Cholinesterases, in addition to their well-known esterase action, also show an aryl acylamidase (AAA) activity whereby they catalyze the hydrolysis of amides of certain aromatic amines. The biological function of this catalysis is not known. Furthermore, it is not known whether the esterase catalytic site is involved in the AAA activity of cholines...

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