Karin Stana-Kleinschek

Karin Stana-Kleinschek
Graz University of Technology | TU Graz · Institute of Chemistry and Technology of Biobased Systems

Prof Dr. Rer. Nat

About

277
Publications
78,325
Reads
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5,853
Citations
Citations since 2016
94 Research Items
3827 Citations
20162017201820192020202120220200400600
20162017201820192020202120220200400600
20162017201820192020202120220200400600
20162017201820192020202120220200400600
Additional affiliations
January 2020 - present
Graz University of Technology
Position
  • Head of Department
October 2019 - present
Graz University of Technology
Position
  • Professor
January 2004 - January 2016
University of Maribor
Position
  • Head of Department

Publications

Publications (277)
Article
Full-text available
Polysaccharide-based scaffolds are promising carriers for enzyme immobilization. Here, we demonstrate a porous scaffold prepared by direct-ink-writing 3D printing of an ink consisting of nanofibrillated cellulose, carboxymethyl cellulose and citric acid for immobilization application. Negative surface charge introduced by the components made the sc...
Article
Full-text available
Herein, we fabricated chemically crosslinked polysaccharide-based three-dimensional (3D) porous scaffolds using an ink composed of nanofibrillated cellulose, carboxymethyl cellulose and citric acid (CA), featuring strong shear thinning behavior and adequate printability. Scaffolds were produced by combining direct-ink-writing 3D printing, freeze-dr...
Article
Full-text available
Cellulose-water interactions are crucial to understand biological processes as well as to develop tailor made cellulose-based products. However, the main challenge to study these interactions is the diversity of natural cellulose fibers and alterations in their supramolecular structure. Here, we study the humidity response of different, well-define...
Article
Full-text available
Stable chitosan thin films can be promising substrates for creating nanometric peptide-bound polyglucosamine layers. Those are of scientific interest since they can have certain structural similarities to bacterial peptidoglycans. Such films were deposited by spin coating from chitosan solutions and modified by acetylation and N-protected amino aci...
Article
Full-text available
The path to greater sustainability and the development of polymeric drug delivery systems requires innovative approaches. The adaptation and use of biobased materials for applications such as targeted therapeutic delivery is, therefore, in high demand. A crucial part of this relates to the development of porous and hollow structures that are biocom...
Article
Full-text available
The biocompatibility of body implants made from polytetrafluoroethylene (PTFE) is inadequate; therefore, the surface should be grafted with biocompatible molecules. Because PTFE is an inert polymer, the adhesion of the biocompatible film may not be appropriate. Therefore, the PFTE surface should be modified to enable better adhesion, preferably by...
Article
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Modification and functionalization of polymer surface properties is desired in numerous applications, and a standard technique is a treatment with non-equilibrium gaseous plasma. Fluorinated polymers exhibit specific properties and are regarded as difficult to functionalize with polar functional groups. Plasma methods for functionalization of polyv...
Article
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The most frequent neutralisation procedure, applied on chitosan (CS) films includes treatment with NaOH base. Such treatment endows CS films with stability in water, yet, same can significantly decrease the film performance. In the present paper, we investigate Mg(OH) 2 nanoparticles as a neutralisation agent for CS solutions followed by casting in...
Article
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The intention of this publication is to give an overview on research related to conjugates of polysaccharides and peptides. Dextran, chitosan, and alginate were selected, to cover four of the most often encountered functional groups known to be present in polysaccharides. These groups are the hydroxyl, the amine, the carboxyl, and the acetal functi...
Article
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As one of the most abundant, multifunctional biological polymers, polysaccharides are considered promising materials to prepare tissue engineering scaffolds. When properly designed, wetted porous scaffolds can have biomechanics similar to living tissue and provide suitable fluid transport, both of which are key features for in vitro and in vivo tis...
Article
Full-text available
We report here a one-step aqueous method for the synthesis of isolated and purified polysaccharide-amino acid conjugates. Two different types of amino acid esters: glycine methyl ester and l-tryptophan methyl ester, as model compounds for peptides, were conjugated to the polysaccharide carboxymethylcellulose (CMC) in water using carbodiimide at amb...
Article
Full-text available
Despite the extensive utilization of polysaccharide hydrogels in regenerative medicine, current fabrication methods fail to produce mechanically stable scaffolds using only hydrogels. The recently developed hybrid extrusion-based bioprinting process promises to resolve these current issues by facilitating the simultaneous printing of stiff thermopl...
Article
Full-text available
In this study, we report the isolation of cellulose nanocrystals (CNCs) from Isora plant fibers by sulfuric acid hydrolysis and their assembly on hydrophilic cellulose and silicon-dioxide (SiO2) surfaces via a layer-by-layer (LBL) deposition method. The isolated CNCs were monodispersed and exhibited a length of 200-300 nm and a diameter of 10-20 nm...
Book
The introduction of the Laboratory Manual gives the theoretical bases on cellulose and its derivatives, which are used as starting polymers for the preparation of multifunctional polymers with three different advanced techniques - spin coating, electrospinning and 3D printing. In the following, each technique is presented in a separate Lab Exercise...
Article
Full-text available
Defluorination of polytetrafluoroethylene (PTFE) surface film is a suitable technique for tailoring its surface properties. The influence of discharge parameters on the surface chemistry was investigated systematically using radio-frequency inductively coupled H2 plasma sustained in the E- and H-modes at various powers, pressures and treatment time...
Article
This study describes the formation of cellulose based polyelectrolyte charge complexes on the surface of biodegradable polycaprolactone (PCL) thin films. Anionic sulphated cellulose (CS) and protonated cationic amino cellulose (AC) were used to form these complexes with a layer-by-layer coating technique. Both polyelectrolytes were analyzed by char...
Article
Full-text available
Alkaline anion exchange membrane fuel cells (AAEMFC) are attracting ever-increasing attention, as they are promising electrochemical devices for energy production, presenting a viable opponent to the more researched proton exchange membrane fuel cells (PEMFCs). Consequently, great progress has been made in the area of designing and developing synth...
Article
Full-text available
Herein, colloidal dispersions of alkaline nano-particles (NPs: CaCO 3 and Mg(OH) 2) are stabilized by trimethylsilyl cellulose (TMSC) in hexamethyldisiloxane and employed to treat historical wood pulp paper by an effortless dip-coating technique. Both alkaline NPs exhibit high stability and no size and shape changes upon stabilization with the poly...
Article
Full-text available
This study presents a simple and straightforward method for the preparation of aqueous-based stable polysaccharide-metal oxide nanoparticles composites-composed of carboxymethyl cellulose (CMC) and titanium dioxide nanoparticles (TiO 2 NPs), and their interaction with the cellulose surface, is presented. Using a combination of anionic polysaccharid...
Article
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The journal “Polysaccharides” is a new open access journal which has attracted an ambitious editorial team, active in different areas of biopolymer—i [...]
Article
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Electrospinning as method for fabrication of wound dressing materials with included medical plant extracts for wound treatment has lately gained increasing attention. However, the transfer of nanofiber fabrication with included plant extracts from the research to the pilot and industrial scale production, using needleless electrospinning is a vital...
Article
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Limitations in wound management have prompted scientists to introduce bioprinting techniques for creating constructs that can address clinical problems. The bioprinting approach is renowned for its ability to spatially control the three-dimensional (3D) placement of cells, molecules, and biomaterials. These features provide new possibilities to enh...
Article
Full-text available
This work concerns freeze-dry processing of CNF aerogels, including aluminum hydroxide trihidrate (Alolt) particles and Sodium silicate, as active and passive flame retardants, respectively. Alkalinity of Sodium silicate promotes stability, dissociation and co-precipitation of Al(OH)3 component onto CNFs. The (auto)fluorescence-enabled confocal mic...
Article
Full-text available
Three-dimensional scaffolds (3D) with controlled shape, dual porosity and long-term mechanical and dimensional stability in biofluids are of interest as biotemplates in tissue engineering. Herein, self-standing and lightweight cellulose-based biogenic scaffolds with a spatially structured morphology, macropores and interconnected micropores were fa...
Article
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Present work describes a novel polymer based nanocomposite anionic exchange membranes (AEMs) with improved features for direct alkaline fuel cell applications. AEMs based on chitosan (CS), magnesium hydroxide (Mg(OH)2) and graphene oxide (GO) with benzyltrimethylammonium chloride (BTMAC), as the hydroxide conductor, were fabricated by a solvent cas...
Article
Full-text available
This work describes the preparation of spin-coated thin polymer films composed of cellulose (CE), ethyl cellulose (EC), and cellulose acetate (CA) in the form of bi- or mono-component coatings on sensors of a quartz crystal microbalance with dissipation monitoring (QCM-D). Depending on the composition and derivative, hydrophilicity can be varied re...
Article
Full-text available
This work describes the preparation of spin-coated thin polymer films composed of cellulose (CE), ethyl cellulose (EC) and cellulose acetate (CA) in the form of bi- or mono-component coatings on sensors of a quartz crystal microbalance with dissipation monitoring (QCM-D). Depending on the composition and derivative, hydrophilicity can be varied res...
Article
The potential of alginate/carboxymethyl cellulose based thin films was demonstrated as a testing platform for evaluation of the influence of in situ incorporated growth factors into formulations intended for 3D bio-printed wound healing materials. Actual formation of blend films with and without included growth factors was analysed using ATR-IR spe...
Article
Full-text available
Stable and (bio)-compatible nanofibrous matrices showing effective incorporation and release of nonsteroidal anti-inflammatory drugs (NSAIDs) hold a huge potential in tissue regeneration and wound healing. Herein, a two-step, water-based and needleless electrospinning method is used to fabricate thermally cross-linked multifunctional nanofibrous su...
Article
This work describes the derivatization of dextran using N-(tert-butyloxycarbonyl)-S-(trityl)-L-cysteine in the presence of N,N'-carbonyldiimidazole (CDI) as a coupling agent. Homogeneous reactions in dimethyl sulfoxide allowed for an efficient coupling of the amino acid derivative to the polymer backbone. Derivatization was confirmed by infrared an...
Article
Full-text available
This work describes the interaction of the human blood plasma proteins albumin, fibrinogen and γ-globulins with micro-and nano-patterned polymer interfaces. Protein adsorption studies were correlated to the fibrin clotting time of human blood plasma and to the growth of primary human endothelial cells (hECs) on these patterns. It was observed that...
Article
3D printed bioscaffolds from polysaccharide materials hold a huge promise in tissue engineering applications, especially in regard to in vitro culturing of pancreatic cells, which require cell-ECM mimicking interactions in all spatial dimensions to remain viable for longer times. Material features, surface characteristics and the physical nature of...
Article
Full-text available
Chronic wounds not only lower the quality of patient's life significantly, but also present a huge financial burden for the healthcare systems around the world. Treatment of larger wounds often requires the use of more complex materials, which can ensure a successful renewal or replacement of damaged or destroyed tissues. Despite a range of advance...
Article
Full-text available
The aim of this study was to develop a material based on cellulose acetate and chitosan fabricated via a simple non-hazardous solvent casting method and modified them by deacetylation approach. Both the modified and unmodified chitosan/cellulose acetate films were well characterized in terms of their morphology, chemical moieties and wettability us...
Article
Development of drug-loaded wound dressings is often performed without systematic consideration of the changing wound environment that can influence such materials’ performance. Among the crucial changes are the wound pH and temperature, which have an immense effect on the drug release. Detailed release studies based on the consideration of these ch...
Chapter
The chemical composition of non-traditional plant fibres depends on their biological origin and age, likewise the extraction method. Chemical composition has a significant impact on the process of isolation of the fibres and their further usability and especially on the fibres’ properties.
Chapter
There are many non-conventional fibre-yielding plants with the potential for use for different applications. Some of them are presented in the chapter.
Chapter
Sustainable materials based on renewable raw materials with new or improved properties are currently the research imperative, however the exploitation of non-traditional, especially wild plants for fibre extraction or for the production of cellulose may be associated with a number of concerns.
Chapter
Some plants growing wild in continental and mediterranean European climates were studied as potential sources of cellulose fibres, i.e. nettle, weeping willow, balm-leaved archangel, lucerne and Spanish broom. Extraction of fibres by enzyme retting and chemical retting, respectively, fibres’ isolation and pre-treatment are described. In addition to...
Chapter
Nanocrystals (CNCs) and cellulose nanofibrils (CNFs) are the two main types of nanocellulose. Recently several non-wood lignocellulosic sources are studied for preparation of cellulose nanomateriales and also many non-traditional sources of cellulose are involved in research, such as soybean stock, swede root, sugar beet pulp, potato tubers, wheat...
Chapter
Plant fibres are elongated, most commonly sclerenchyma supportive plant cells with thick cellulose walls with a well-organised structure. Cellulose natural fibres are characterised by a fibrillar structure in which the crystalline arranged parts of cellulose I form a periodic array with amorphous cellulose molecules. The morphological hierarchy is...
Book
The authors describe how sustainable textile fibers from crops such as quinoa, grass, hops, corn and wheat stems, etc. have recently begun to generate great interest. The structure-property relationships of such non-conventional cellulose fibers are studied in this brief, as are their sorption and surface properties which are of primary importance....
Article
Full-text available
Novel multifunctional bio-based nanofibrous mats were prepared which contain the commonly used pain reducing local anesthetic benzocaine (BZC) and the in situ pH-detecting dye bromocresol green (BCG). These can serve as a dual nano-carrier system for wound healing applications, especially in the treatment of infected wounds. BZC and BCG were introd...
Article
Full-text available
This paper aims at a better understanding of the systematic production of the ethyl cellulose (EC) particles by using an emulsification-solvent evaporation method in the presence of different polysaccharide derivatives. In particular, the role of different surface active polymers i.e. one ionic—carboxymethyl cellulose, one nonionic—hydroxyethyl cel...
Article
Full-text available
Pain is already known to cause delays in wound healing. Therefore, providing suitable therapeutic solutions for less painful wound healing should attract significantly more attention in the development of future novel wound care solutions. In this study, the nonsteroidal anti-inflammatory drug (NSAID) diclofenac sodium (DCS) and the local anestheti...
Article
Control over the mechanical properties of cellulose-based artifacts (e.g. paper) is highly important in cultural heritage science. Especially, a non-aqueous method that does not cause swelling but can be applied to either single paper sheets or bound cellulose items (e.g., books) without any preselection is currently needed. Herein, we present a ne...
Article
The requirement of promoting a revolution in filtration technology has led to growing devotion in advanced functional materials such as electrospun membranes for filtering devices as a solution for providing water at lower energy costs. In this study, electrospun polyvinylidene fluoride membranes were fabricated by reinforcing 0.5 and 1 wt. % of ch...