Karim shokry MohammedCairo University | CU · Department of Microbiology
Karim shokry Mohammed
Master of Microbiology
Teaching assistant at Microbiology department, Cairo University
About
5
Publications
599
Reads
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11
Citations
Introduction
My research is directed to Cancer therapy by targetted nanobodies, and Microbiological and immunological studies. My interest is about cloning and expression techniques and everything associated with molecular biology, I am working right now in a research project called (Cancer therapy by plasmonic photothermal therapy using gold nanorods after near infrared radiation).
Additional affiliations
June 2021 - January 2022
Position
- Lecturer assistant
Description
- I am a Lecturer assistant in Microbiology and Immunology department. My research interest is directed to the gene cloning , protein expression and different Molecular biology techniques. I have a good experience in Microbiological techniques as bacterial and mycological isolation and serological methods as precipitation, agglutination, ELISA and western blot. My profession is teaching the bachelor students the practical sessions in Microbiology and Immunology with advanced teaching techniques.
Education
September 2012 - September 2017
Publications
Publications (5)
Molecular diagnosis of helicobacters by PCR is simpler, more accurate, and feasible compared to other diagnostic methods. Validity and accuracy are highly dependent on the PCR primer design, diffusion time, and mutation rate of helicobacters. This study aimed to design 16srRNA-specific primers for Helicobacter spp. and H. pylori. Application of com...
Background
Helicobacter pylori causes the most common human gastric infection. H. pylori Stool Antigen Lateral Flow Immunochromatography assay (HpSA-LFIA) is considered one of the most cost-effective and rapid non-invasive assays (active tests). Evaluating HpSA-LFIA is of crucial for ensuring accuracy and utility assurance. This study aimed to eval...
Human epidermal growth factor receptor 2 (HER2) is an important prognostic marker in the case of breast cancer and other tumors. Canines remain one of the most important models for comparative oncology with humans due to the great similarity in the spontaneous presentation and development of cancer, and in the high homology in the amino acid sequen...
24 Molecular diagnosis of helicobacters by PCR is simpler, more accurate, and feasible compared to 25 other diagnostic methods. Validity and accuracy are highly dependent on the PCR primer design, 26 diffusion time, and mutation rate of helicobacters. This study aimed to design 16srRNA-specific 27 primers for Helicobacter spp. and H. pylori. Applic...
Molecular diagnosis of helicobacters by PCR is simpler, more accurate, and feasible compared to other diagnostic methods. Validity and accuracy are highly dependent on the PCR primer design, diffusion time, and mutation rate of helicobacters. This study aimed to design 16srRNA -specific primers for Helicobacter spp. and H. pylori . Application of c...
Questions
Questions (4)
Vector pET expression vector 5738 bp
Insert : 3700 bp
transformation :Bl21 chemically competent cells .
Restriction enzymes : Not1 , Pst1
Ligation molar ratio : 1:1
after ligation & transformation i performed Miniprep & another restriction by the same enzymes
I expected to find 2 bands in the range 3700 & 5700 , but i find 2 unexpected bands 9000 bp & 3700 band
I wonder if the 9000 bands is my ligated desired plasmid why the insert band is appearing on the gell afer transformation , Miniprep ?
Can I save my DH5alpha cells in -40 instead of -80?
Is that possible that Nanobodies can be produced as recombinant protein using plasmid expressed in E.coli or is it better to use phage display technique ?
Is it possible that DNA concentration of sample extracted by Qiagen kit comes by negative sign -64 ng/ul and so the absorbance A260 & A280?
A260=-1.299
A280=-0.333
A260/280=3.9
A260/A230=-0.49
