Karim Mohamed Fawzy El-Sayed

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Cairo University | CU · Faculty of Oral and Dental Medicine

This study investigated the periodontal regenerative potential of gingival margin-derived multipotent postnatal stem/progenitor cells. Periodontal defects were induced at six sites in eight miniature pigs in the premolar/molar area (-4 weeks). Autologous cells isolated from the gingival margin were magnetically sorted using STRO-1 antibodies and ch...

Recently, gingival margin-derived stem/progenitor cells isolated via STRO-1/magnetic activated cell sorting (MACS) showed remarkable periodontal regenerative potential in vivo. As a second-stage investigation, the present study's aim was to perform in vitro characterisation and comparison of the stem/progenitor cell characteristics of sorted STRO-1...

The present study investigated the periodontal regenerative potential of gingival margin-derived stem/progenitor cells (G-MSCs) in conjunction with IL-1ra releasing hyaluronic acid synthetic extracellular matrix (HA-sECM). Periodontal defects were induced at four sites in eight miniature-pigs in the premolar/molar area (-4 weeks). Autologus G-MSCs...

The human gingiva, characterized by its outstanding scarless wound healing properties, is a unique tissue and a pivotal component of the periodontal apparatus, investing and surrounding the teeth in their sockets in the alveolar bone. In the last years gingival mesenchymal stem/progenitor cells (G-MSCs), with promising regenerative and immunomodula...

Objective: The objective of this randomized controlled trial was to evaluate the radiographic changes and histologic healing following alveolar ridge preservation (ARP) using autogenous whole tooth (AWTG), test group versus demineralised dentin (ADDG), control group. Material and methods: 20 non-molar teeth indicated for extraction were randomized...

Dental implants are crucial in contemporary oral rehabilitation, necessitating optimal integration with the surrounding soft tissues for durable success. The attachment between the implant surface and peri-implant mucosa should establish a secure seal to prevent bacterial infiltration and subsequent tissue inflammation. This concise review examines...

Background: As a unique joint that facilitates the articulation of the upper and lower jaws, the temporomandibular joint (TMJ) is concerned with several critical functions, such as speech and mastication. Pain that can become incapacitating is a result of temporomandibular disorders (TMDs), which are complex disorders affecting the masticatory musc...

Objective The present trial evaluated the first‐time application of autogenous demineralized dentin graft with injectable platelet‐rich fibrin (ADDG + i‐PRF) versus autogenous demineralized dentin graft (ADDG), in alveolar ridge preservation (ARP) in the maxillary aesthetic zone. Material and Methods Twenty‐two maxillary ( n = 22) non‐molar teeth...

Aim Injectable platelet‐rich fibrin (I‐PRF), a second‐generation platelet concentrate, is widely used to enhance soft and hard tissue healing alone or in combination with biomaterials, relying on its harboring of various pivotal growth/differentiation factors. This randomized trial assessed the effect of clindamycin (CLN) augmented injectable plate...

Objectives The present trial’s aim was to compare the remineralization potential of self-assembling peptide P11-4 combined with fluoride to that of fluoride varnish. Materials and methods Twenty-eight participants with 58 incipient carious lesions were enrolled in the present trial. Participants were randomly divided into two groups with 14 partic...

Aims: Oxidized low-density lipoprotein (oxLDL) is an important player in the course of metabolic inflammatory diseases. oxLDL was identified in the gingival crevicular fluid, denoting possible associations between oxLDL-induced inflammation and periodontal disease. The current investigation compared for the first-time direct effects of oxLDL to a c...

Objectives Esthetic crown lengthening (ECL) is commonly advocated to treat patients with altered passive eruption (APE). Since the introduction of the minimally invasive surgical concept, a limited number of studies have investigated this technique in a standardized manner, with further studies required to verify the validity and predictability of...

Aims This randomized trial assessed for the first time the efficacy of coronally advanced flap (CAF) followed by micro‐needling (MN) in contrast to CAF with acellular dermal matrix (ADM) on gingival thickness (GT, primary outcome), keratinized tissue width (KTW), clinical attachment level (CAL), probing depth (PD), recession depth (RD), recession w...

Dental mesenchymal stem cells (MSCs) constitute a promising candidate for tissue regeneration in regenerative medicine. While cellular transplantation of MSCs has shown potential for achieving functional tissue regeneration, several clinical challenges hinder its implementation. An alternative approach, harnessing the secretome of MSCs, has gained...

The present study explores for the first time the effect of hyperbaric oxygen (HBO) on gingival mesenchymal stem cells’ (G-MSCs) gene expression profile, intracellular pathway activation, pluripotency, and differentiation potential under an experimental inflammatory setup. G-MSCs were isolated from five healthy individuals (n = 5) and characterized...

Periodontitis is a complex inflammatory disorder of the tooth supporting structures, associated with microbial dysbiosis, and linked to a number if systemic conditions. Untreated it can result in an irreversible damage to the periodontal structures and eventually teeth loss. Regeneration of the lost periodontium requires an orchestration of a numbe...

Background: Stem/progenitor cells from human exfoliated deciduous teeth (SHED) show remarkable pluripotency, regeneration and immunological capacities. During in-vivo regenerative events, SHED could crosstalk with the surrounding inflammatory microenvironment, through toll-like-receptors (TLRs). Aim: The current investigation depicts a character...

Objectives Sonic/ultrasonic devices are essential tools in today’s endodontics. This prospective trial evaluated for the first time the impact of practitioners’ proficiency levels and patient-related factors on complications associated with a high frequency polyamide sonic irrigant activation device. Methods In total 334 patients (females:158, mal...

Aim The current randomized controlled clinical trial assessed the effect of injectable platelet-rich fibrin (I-PRF) combined with demineralized freeze-dried bone allograft (DFDBA) compared to DFDBA alone in the management of intrabony defects of stage-III periodontitis patients. Methodology Following sample size calculation, twenty stage-III perio...

Periodontitis is the sixth most common chronic inflammatory disease, destroying the tissues supporting the teeth. There are three distinct stages in periodontitis: infection, inflammation, and tissue destruction, where each stage has its own characteristics and hence its line of treatment. Illuminating the underlying mechanisms of alveolar bone los...

Strukturierte Mundhygieneinstruktion bei der Parodontitisbehandlung-eine explorative Studie Warum Sie diesen Beitrag lesen sollten Obwohl die erste Therapiestufe sowohl in der gängigen Praxis als auch in den aktuellen europäischen Leitlinien die Mund-hygieneinstruktion inklusive der Anleitung zur Anwendung von Interdentalraumbürsten beinhaltet, ist...

Nanocomposite biomaterials combine a biopolymeric matrix structure with nanoscale fillers. These bioactive and easily resorbable nanocomposites have been broadly divided into three groups, namely natural, synthetic or composite, based on the polymeric origin. Preparing such nanocomposite structures in the form of hydrogels can create a three-dimens...

Cellular deficiency in periodontal and peri-implant diseases represents the major challenge in treatment outcomes. Cell sheet therapy is an approach by which cell-to-cell communication through endogenous matrix secretion offers a good modification to increase the reliability of traditional cell therapy. In this study, sandwiching the rapidly resorb...

Aim: The current randomized controlled clinical trial assessed the effect of injectable platelets rich fibrin (I-PRF) combined with demineralized freeze-dried bone allograft (DFDBA) compared to DFDBA alone in the management of intrabony defects of stage-III periodontitis patients. Methodology: Following sample size calculation, twenty stage-III per...

Aim The current randomized controlled trial assessed for the first time the effect of a low-speed platelet-rich fibrin (PRF) with open flap debridement (OFD) versus OFD alone in the treatment of periodontal intra-osseous defects of stage-III periodontitis patients. Methods Twenty-two periodontitis patients with ≥ 6 mm probing depth (PD) and ≥ 3 mm...

Objective: The present randomized controlled trial compares for the first time the vestibular socket therapy (VST) to the contour augmentation technique in the management of compromised fresh extraction sockets in the maxillary esthetic zone, regarding mid-facial soft tissue changes (primary outcome), mesial and distal papillae dimensions, horizon...

Vitamins and omega-3 fatty acids (Ω3FA) modulate periodontitis-associated inflammatory processes. The aim of the current investigation was to evaluate associations of oral nutrient intake and corresponding serum metabolites with clinical severity of human periodontitis. Within the Food Chain Plus cohort, 373 periodontitis patients—245 without (POL)...

Thymoquinone (TQ), the key active component of Nigella sativa (NS), demonstrates very promising biomedical anti-inflammatory, antioxidant, antimicrobial and anticancer properties. Several investigations have inspected the modulative activities of TQ on different stem/progenitor cell types, but its possible role in the regulation of gingival mesench...

Introduction: According to the actual EFP guidelines, first step of periodontal therapy should include oral hygiene instructions (OHI), comprising the use of interdental brushes (IDBs). Yet, non-conclusive evidence exists for their effect. The present multicentric clinical controlled explorative study compared the clinical outcomes of two NSPT (non...

Mesenchymal stem/progenitor cells (MSCs) are prospective cellular candidates for numerous regenerative and immunotherapeutic purposes. Their immunomodulatory potential and multilineage abilities allow them to be deployed for the treatment of various conditions. Nevertheless, depending on the local microenvironment, different biological tasks of MSC...

Objective: Although the therapeutic effects of nonsurgical periodontal therapy (NSPT) are well established, the clinical benefits of the additional use of periodontal endoscopy (PE) remain controversial. Therefore, this randomized controlled split-mouth pilot study evaluated the effect of NSPT using PE versus NSPT without nPE on bleeding on probin...

Regenerative medicine research provides a new hope in treatment of patients suffering from severe injuries or chronic diseases. The increased interest in stem/progenitor cells is encouraged by their biological nature for self-renewal and multilineage differentiation potential. Cornerstones for tissue engineering are stem/progenitor cells, scaffolds...

Dental mesenchymal stem/progenitor cells (MSCs) have unique biological criteria in terms of genes and protein expression. They possess the common characteristics of non-dental MSCs that include the self-renewal ability, differentiation potential into variable cell lineages, and immunomodulatory properties. In addition, they reveal superior regenera...

Objectives To characterize human-gingival-fibroblast-(HGFs) viability, proliferation and adhesion on polymer-infiltrated-ceramic-network-(PICN), polyetheretherketone-(PEEK), hydroxyapatite-reinforced-polyetheretherketone-(HA-PEEK), polyetherketoneketone-(PEKK), as well as conventional titanium-(Ti) and zirconia ceramic-(Zr) implant materials in-vit...

Citation: Fawzy El-Sayed, K.M.; Bittner, A.; Schlicht, K.; Mekhemar, M.; Enthammer, K.; Höppner, M.; Es-Souni, M.; Schulz, J.; Laudes, M.; Graetz, C.; et al. Ascorbic Acid/Retinol and/or Inflammatory Stimuli's Effect on Proliferation/Differentiation

Aim To assess platelet-rich fibrin (PRF) with ascorbic acid (AA) versus PRF in intra-osseous defects of stage-III periodontitis patients. Methodology Twenty stage-III/grade C periodontitis patients, with ≥ 3 mm intra-osseous defects, were randomized into test (open flap debridement (OFD)+AA/PRF; n = 10) and control (OFD+PRF; n = 10). Clinical atta...

Objective The current study aimed to evaluate the effect of electronic cigarette (EC) aerosol, Cannabis, and conventional cigarettes smoke on gingival fibroblast/gingival mesenchymal stem cells' (GF/G-MSCs) of never smokers. Material and Methods Human GF/G-MSCs (n = 32) were isolated and characterized using light microscopy, flow cytometry, and mu...

Regenerative dentistry has paved the way for a new era for the replacement of damaged dental tissues. Whether the causative factor is dental caries, trauma, or chemical insult, the loss of the pulp vitality constitutes one of the major health problems worldwide. Two regenerative therapies were introduced for a fully functional pulp-dentin complex r...

Aim The present study aimed to systematically assess current evidence on effects of locally delivered antibiotics during periodontal surgery compared to periodontal surgery alone on clinical attachment level (CAL) gain, probing pocket depth (PPD) reduction, recession depth (RD) changes, gingival index (GI), bleeding on probing (BOP), and plaque ind...

This study compared endodontic access cavities prepared by operators of different experience levels (students, general‐practitioners and specialists), guided by periapical radiographs, cone‐beam computed tomography (CBCT) or 3D CBCT‐based planning software, with regards to tooth substance loss and preparation errors. Operators (n = 34) prepared end...

Mesenchymal stem/progenitor cells (MSCs) have a multi-differentiation potential into specialized cell types, with remarkable regenerative and therapeutic results. Several factors could trigger the differentiation of MSCs into specific lineages, among them the biophysical and chemical characteristics of the extracellular matrix (ECM), including its...

Objectives: Ascorbic-acid and Retinol (AA/Retinol) application could drive cellular reprogramming, pluripotency and regeneration. As gingival mesenchymal stem/progenitor cells (G-MSCs)-mediated periodontal regeneration would occur under inflamed micro-environmental conditions, the present investigation explored for the first time the combined effec...

Being driver of cell renewal and differentiation, gingival mesenchymal stem cells (G-MSCs) are key players in periodontal regeneration. Thus, investigating MSC differentiative properties has become a much-noticed issue. Requiring a three-dimensional setting, no visual quantification of chondrogenic differentiation has been established yet. Here we...

Background The ability to restore missing teeth with dental implants is dictated by the available bone and by the presence of anatomical structures. The potential to insert ultrashort implants avoids additional surgical procedures and its inherent complications. The last European Association of Dental Implantologists consensus in 2016 defined ultra...

Die klinischen Vorteile der parodontalen Endoskopie (PE) als zusätzliche Visualisierungsmöglichkeit subgingival in der nichtchirurgischen Parodontaltherapie (NSPT) werden kontrovers diskutiert, auch weil ein zusätzlicher klinischer Nutzen durch die Visualisierung harter Auflagerungen (HD) in der NSPT bisher nicht oder nur begrenzt nachgewiesen werd...

Dentin-pulp complex is a term which refers to the dental pulp (DP) surrounded by dentin along its peripheries. Dentin and dental pulp are highly specialized tissues, which can be affected by various insults, primarily by dental caries. Regeneration of the dentin-pulp complex is of paramount importance to regain tooth vitality. The regenerative endo...

Objective. Ascorbic acid (AA) and controlled inflammatory stimuli are postulated to possess the ability to independently exert positive effects on a variety of proliferative, pluripotency, and differentiation attributes of gingival mesenchymal stem/progenitor cells (G-MSCs). The current study’s objective was to explore and compare for the first tim...

Mesenchymal stem/progenitor cells (MSCs) are key players in regenerative medicine, relying principally on their differentiation/regeneration potential, immunomodulatory properties, paracrine effects, and potent homing ability with minimal if any ethical concerns. Even though multiple preclinical and clinical studies have demonstrated remarkable pro...

Objectives Stem/progenitor Cells form the Apical Papilla (SCAP) demonstrate remarkable regenerative and immunomodulatory properties. During their regenerative events, SCAP, similar to other stem/progenitor cells could interact with their local inflammatory micro-environment via their expressed toll-like-receptors (TLRs). The present study aimed to...

Aim Toll‐like‐receptors are key players in mesenchymal stem/progenitor cells’ micro‐environmental crosstalk, endorsing various biological reactions. For the first, time this study investigates the effects of TLR3‐ligation on gingival mesenchymal stem/progenitor cells (G‐MSCs) stemness and differentiation properties. Material and Methods G‐MSCs (n=...

Objectives This randomized controlled trial compares for the first time effects of Alvogyl versus absorbable gelatin sponge as palatal wound dressings on postoperative pain, amount of analgesic consumption, post-surgical bleeding, and wound re-epithelization.Materials and methodsFollowing sample size calculation, 36 systemically healthy patients re...

Cell-based therapies currently represent the state of art for tissue regenerative treatment approaches for various diseases and disorders. Induced pluripotent stem cells (iPSCs), reprogrammed from adult somatic cells, using vectors carrying definite transcription factors, have manifested a breakthrough in regenerative medicine, relying on their plu...

Environmental conditions, including oxygen pressure and inflammation, could affect G-MSCs periodontal regenerative potential. Hyperbaric oxygen (HBO) is known for increasing tissue regeneration/repair and modulating mesenchymal stem cells' pluripotency and proliferation. This study's aim was to explore the HBO/inflammation related effects on G-MSCs...

Effect of low versus conventional speed drilling on maxillary dental implants’ success: A randomized controlled clinical trial Background: Endosseous dental implants’ placement is fundamental for prosthetic reconstruction of partially or completely edentulous maxilla, frequently demonstrating insufficient bone volume and quality in vertical and/o...

Objectives This randomized controlled trial compares for the first time effects of Alvogyl versus absorbable gelatin sponge as palatal wound dressings on postoperative pain, amount of analgesic consumption, post-surgical bleeding, and wound re-epithelization. Materials and methods Following sample size calculation, 36 systemically healthy patients...

Stem/progenitor cells are undifferentiated cells characterized by their exclusive ability for self-renewal and multilineage differentiation potential. In recent years, researchers and investigations explored the prospect of employing stem/progenitor cell therapy in regenerative medicine, especially stem/progenitor cells originating from the oral ti...

Oral mesenchymal stem/progenitor cells (MSCs) are renowned in the field of tissue engineering/regeneration for their multilineage differentiation potential and easy acquisition. These cells encompass the periodontal ligament stem/progenitor cells (PDLSCs), the dental pulp stem/progenitor cells (DPSCs), the stem/progenitor cells from human exfoliate...

Stem/progenitor cells are undifferentiated cells characterized by their exclusive ability for self-renewal and multilineage differentiation potential. In recent years, researchers and investigations explored the prospect of employing stem/progenitor cell therapy in regenerative medicine, especially stem/progenitor cells originating from the oral ti...

Background. In the field of periodontal guided tissue regeneration, microperforated membranes have recently proved to be very promising periodontal regenerative tissue engineering tools. Regenerative periodontal approaches, employing gingival mesenchymal stem/progenitor cells in combination with these novel membranes, would occur mostly in inflamed...

Regenerative medicine literature has proposed mesenchymal stem/progenitor cell- (MSC-) mediated therapeutic approaches for their great potential in managing various diseases and tissue defects. Dental MSCs represent promising alternatives to nondental MSCs, owing to their ease of harvesting with minimally invasive procedures. Their mechanism of act...

Background: Stem/progenitor cells-mediated pulpal regeneration could represent a promising therapeutic alternative in the field of clinical endodontics. Aim: The present study aimed to systematically assess and meta-analyse dental pulpal tissue regeneration, pulpal vitality and apical healing after stem/progenitor cells' transplantation versus n...

Objectives: In recent years, gingival mesenchymal stem/progenitor cells (G-MSCs) displayed substantial tissue regenerative potential and progressive experimental clinical outcomes. Regenerative capacities of MSCs can be altered by different factors, including the stimulation by specific ligands as TLR3 ligand Poly (I:C). The objective of the presen...