Karen BräutigamUniversity Hospital Schleswig-Holstein, Lübeck, Germany · Department of Gynecology and Obstetrics
Karen Bräutigam
Dr. rer. nat.
About
55
Publications
5,837
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
1,139
Citations
Introduction
Publications
Publications (55)
Background
Ficus umbellata Vahl is one of the plants used in Cameroonian empirical system to treat cancer. Previous studies have shown its cytotoxic potential on mammary and ovarian cancer cells, and its chemopreventive potential against ovarian cancer in rats. The aim of this work was to determine the bioactive constituents of F. umbellata Vahl an...
Objective: The objective of the study is to validate a new human papillomavirus (HPV) L1 high-risk specific serological assay in a case-control study.
Methods: Serum samples of 138 patients (cervical intraepithelial neoplasia (CIN) 1, 2, and 3 and cervical cancer), 21 vaccinees, and 246 female controls were tested for the presence of HPV L1 high-ri...
Introduction: PD1/PD-L1 inhibition (ICi) has recently become a new standard of care for patients with advanced MMR-deficient (MMRd) endometrial cancers. Nevertheless, response to immunotherapy is more complex than the presence of a single biomarker and therefore it remains challenging to predict patients response to ICi beyond MMRd tumors. Elevated...
Background/aim:
Cervical cancer is the fourth most common cause of cancer-related deaths in women worldwide. The potential for targeted therapy against the immune checkpoint programmed death 1 (PD-1)/programmed death-ligand 1 (PD-L1) and receptor tyrosine kinases was examined in cervical cancer patients and cell lines.
Materials and methods:
On...
Background:
Cancers and intraepithelial lesions of different anogenital areas as well as oral cancer are associated with human papilloma virus (HPV) infections.
Methods:
In this study cervical, vaginal, vulvar, anal, and oral samples were taken from 509 patients visiting our dysplasia consultation clinic. HPV genotyping was performed using the E...
Purpose
Triple-negative breast cancer (TNBC) is characterized by an unfavorable prognosis and missing systemic therapeutic approaches beside chemotherapy. Targeting the immune checkpoint PD-1/PD-L1 showed promising results in breast cancer and especially in TNBC. The extracellular signal-regulated kinase 1/2 (ERK1/2) is an important driver of carci...
Purpose: Triple negative breast cancer (TNBC) is characterized by an unfavorable prognosis and missing systemic therapeutic approaches beside chemotherapy. Targeting the immune checkpoint PD-1/PD-L1 showed promising results in breast cancer and especially in TNBC. The extracellular signal-regulated kinase 1/2 (ERK1/2) is an important driver of carc...
In the last few decades, antibody-based diagnostic and therapeutic applications have been well established in medicine and have revolutionized cancer managements by improving tumor detection and treatment. Antibodies are unique medical elements due to their powerful properties of being able to recognize specific antigens and their therapeutic mecha...
Purpose:
Human papilloma virus (HPV) as the most common viral infection of the anogenital tract is highly associated with intraepithelial neoplasia and cancer of the cervix and other anogential regions. To date, 15 high-risk (HR-) HPV and 3 probably/possibly HR-HPVs have been found to be associated with cervical cancer. Therefore, a screening espe...
Antibody-based diagnostic and therapeutic reagents armed with effector molecules such as dyes and drugs offer hope in the battle against cancer. Several site-specific conjugation methods have been developed to equip antibodies with such effector molecules, but they tend to be expensive and involve multiple reaction steps. The conjugation of two dif...
Purpose:
Targeted theranostics is an alternative strategy in cancer management that aims to improve cancer detection and treatment simultaneously. This approach combines potent therapeutic and diagnostic agents with the specificity of different cell receptor ligands in one product. The success of antibody drug conjugates (ADCs) in clinical practic...
Triple-negative breast cancer (TNBC) is a heterogeneous disease in which the tumors do not express estrogen receptor (ER), progesterone receptor (PgR) or human epidermal growth factor receptor 2 (HER2). Classical receptor-targeted therapies such as tamoxifen or trastuzumab are therefore unsuitable and combinations of surgery, chemotherapy and/or ra...
Background/aim:
Diagnosis of triple-negative breast cancer (TNBC) is associated with adverse prognosis, particularly in cases of chemotherapy resistance. The goal of this analysis was to compare TNBC vs. non-TNBC cell lines and those of distinct TNBC subtypes with regard to sensitivity to eribulin in vitro.
Materials and methods:
Breast cancer c...
Purpose:
The term "theranostics" represents a new paradigm in medicine especially for cancer treatment. This term was coined by Funkhouser in 2002 and defines a reagent that combines therapeutic and diagnostic properties. It is widely believed that theranostics agents will have considerable impact on healthcare before, during, and after disease by...
Die Diagnose des Triple-negativen Mammakarzinoms (TNBC) ist mit einer ungunstigen Prognose gekennzeichnet, die im Gegensatz zur erhohten Wahrscheinlichkeit eines Ansprechens auf (neoadjuvante) Chemotherapie steht. Subgruppen-Analysen klinischer Studien suggerieren eine erhohte Ansprechrate Triple-negativer Tumore gegenuber Eribulin. Ziel der Unters...
Introduction:
Diagnosis of triple negative breast cancer (TNBC) is associated with adverse prognosis particularly in case of chemotherapy resistance. TNBC is a heterogeneous entity and seems to consist of at least six distinct molecular subtypes (Lehman subtypes) with distinct chemotherapy sensitivity. The cytotoxic agent eribulin induces tumor cel...
Fatty acid synthase (FASN) is crucial to de novo long-chain fatty acid synthesis, needed to meet cancer cells' increased demands for membrane, energy, and protein production.
We investigated FASN overexpression as a therapeutic and chemosensitization target in ovarian cancer tissue, cell lines, and primary cell cultures. FASN expression at mRNA and...
Fragestellung: Das Ovarialkarzinom steht fur die fatalste Erkrankung gynakologischer Malignome. Grunde fur die schlechte Prognose sind spate Diagnosestellung und eine hohe Rezidivrate, weswegen die Detektion neuer prognostischer Marker und die Entwicklung gezielter Therapien unabdingbar sind. Viele Arbeiten befassen sich daher mit der Identifizieru...
Einfuhrung: Beim metastasierten Mammakarzinom stellt sich die Herausforderung der Ausdehnungsdiagnostik, des Therapie-Monitorings und der moglichst zielgerichteten Therapie. Hierbei kann das Konzept der Photoimmuntherapie als neuartige Strategie eine simultane Diagnostik und Behandlung im Sinne eines Theranostics ermoglichen. Jedoch ist die Herstel...
Objective:
To study altered hemopexin concentrations in peritoneal fluid (PF) samples from patients with endometriosis. Recent data implicate a role of altered iron metabolism in endometriosis patients. Hemopexin is the major transport protein for heme. Like iron, heme exposure to the epithelial surface can provoke oxidative stress on the peritone...
Ovarian cancer has the worst prognosis among all gynecological cancers mainly due to resistance to platinum-containing therapeutics and the lack of an effective screening method for the detection of early stage disease. In order to reduce the mortality of ovarian cancer it will be necessary to overcome the chemoresistance with novel targeted therap...
About 20% of ovarian carcinomas show alterations of 19p13 and/or 19q13 in the form of added extra material whose origin often is from chromosome 11. Based on earlier spectral karyotype analysis of the ovarian cancer cell line SKOV-3, which shows an unbalanced translocation der(19)t(11;19), the aim of this study was to determine the precise breakpoi...
Purpose:
Cancer of the ovary confers the worst prognosis among women with gynecological malignancies, primarily because most ovarian cancers are diagnosed at late stage. Hence, there is a substantial need to develop new diagnostic biomarkers to enable detection of ovarian cancer at earlier stages, which would confer better prognosis. In addition,...
Fragestellung: Epigenetische Modifikationen der DNA spielen eine wichtige Rolle in der Karzinogenese. Eine umfassende, Chip gestutzte Analyse von DNA Methylierungmuster spezieller CpG Sites sollte auf eine mogliche Korrelation mit dem Progressionsfreien Intervall (PFI) von Ovarialkarzinom Patientinnen untersuchen werden. Methode: Von zwanzig fortge...
Many patients with ovarian cancer disease relapse within 6 months after adjuvant chemotherapy, with a limited prognosis. Epigenetic modifications have been shown to play an important role in tumor development and formation. Therefore, global analysis of DNA methylation patterns might reveal specific CpG sites that correlate with progression-free in...
Ovarian cancer accounts for the highest mortality among all gynecological cancers, mainly due to the fast developing chemoresistance. The death ligand TRAIL induces apoptosis and is able to sensitize tumor cells to cytostatic drugs without affecting physiological tissue. Combined treatment of TRAIL and the antidiabetic acting PPARγ ligands was show...
Bedingt durch Chemotherapieresistenz und das Fehlen einer Methode zur Früherkennung hat das Ovarialkarzinom die ungünstigste Prognose unter den gynäkologischen Malignomen. Um die Mortalität zu senken gilt es, Methoden für die Früherkennung und zur Kontrolle des Therapieerfolges zu etablieren. Bildgebende Verfahren haben sich als ungeeignet erwiesen...
The mouse strain CBA/CaH-T(14;15)6Ca/J carries a homozygous balanced reciprocal translocation between mouse chromosomes 14 and 15, but the break points of this translocation have not previously been examined in detail. Using fluorescent in situ hybridization, we assigned the break point in 14qE3 to a 200-kb region devoid of any known gene. We simil...
New strategies in the therapy for malignant diseases depend on a targeted influence on signal transduction pathways that regulate proliferation, cell growth, differentiation, and apoptosis by the activation of serine/threonine kinases. Enzastaurin (LY317615.HCl), a selective inhibitor of protein kinase Cbeta (PKCbeta), is one of these new drugs and...
Objective:
Often not single but multiple genes are indicative for a certain cancer diseases. Various multi-genes are under review of their validity in making a decision for effective treatment, like the increased expression of Aurora kinases in many solid cancers. In this study we take their relative expression to other genes as an additional dime...
Objective:
Aurora kinases (Aurk) are involved in organizing the mitotic spindle apparatus, monitoring the correct chromosome segregation and are required for the process of cytokinesis. In many cancer entities including breast and ovarian cancer, AurkA and AurkB are overexpressed. AZD1152-HQPA is an aurora kinase inhibitor with enhanced selectivit...
Ovarian cancer carries the worst prognosis among all gynaecological cancers, mainly due to the chemoresistance and the lack of an effective screening method for the detection of early stage disease. Early detection will be necessary to reduce the mortality of ovarian cancer. Since it has a low incidence, any screening method has to exhibit a specif...
NOD2 is an intracellular receptor for the bacterial cell wall component muramyl dipeptide. Mutations in the leucine-rich repeat region of NOD2, which lead to an impaired recognition of muramyl dipeptide, have been associated with chronic inflammatory diseases of barrier organs such as Crohn disease, asthma and atopic eczema. In this study we identi...
16030
Background: Enzastaurin (LY317615.HCl), a selective inhibitor of protein kinase C beta II (PKCβII), leads to apoptosis and suppression of angiogenesis and represents a promising drug for clinical development. The effects of Enzastaurin were investigated utilizing the ovarian cancer cell line HEY and several chemoresistant subclones. Methods:...
Cancer of the ovary confers the worst prognosis among women with gynecologic malignancies, underscoring the need to develop new biomarkers for detection of early disease, particularly those that can be readily monitored in the blood.
We developed an algorithm to identify secreted proteins encoded among approximately 22,500 genes on commercial oligo...
20037
Background: Enzastaurin (LY317615.HCl), a selective inhibitor of protein kinase C beta (PKCβ), inhibits induction of angiogenesis and apoptosis. Activation of PKCβ has been correlated with tumor cell proliferation and invasiveness. The impact of Enzastaurin was investigated in an ovarian cancer tissue culture model utilizing the parental cell...
The pro-inflammatory cytokine tumor necrosis factor-alpha (TNF-alpha) plays a central role in inflammatory disorders. Transmembrane TNF-alpha and its two receptors are cleaved by the proteinase TNF-alpha converting enzyme (TACE), resulting in appreciable serum levels of soluble TNF-alpha and soluble TNF-alpha receptors (sTNFR1 and -2). The only kno...
NOD2, a member of the NOD1/Apaf-1 family, was recently identified as the first susceptibility gene for Crohn's disease. The aim of this report was to describe the regulation and functional significance of NOD2 expression in intestinal epithelial cells.
Expression of NOD2 messenger RNA was determined by reverse-transcription polymerase chain reactio...
Background & Aims: NOD2, a member of the NOD1/Apaf-1 family, was recently identified as the first susceptibility gene for Crohn's disease. The aim of this report was to describe the regulation and functional significance of NOD2 expression in intestinal epithelial cells. Methods: Expression of NOD2 messenger RNA was determined by reverse-transcript...
