Karam Pal Singh

Karam Pal Singh
Indian Veterinary Research Institute | IVRI · Centre for Animal Disease Research and Diagnosis

B.V.Sc., M.V.Sc., Ph.D

About

178
Publications
181,885
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2,660
Citations
Additional affiliations
October 2007 - July 2016
Indian Veterinary Research Institute
Position
  • Principal Investigator

Publications

Publications (178)
Article
Full-text available
We report an incidence of natural infection of SARS-CoV-2 in free-ranging Indian leopard (Panthera pardus fusca). The case was detected during routine screening. Post-mortem and laboratory examination suggested virus-induced interstitial pneumonia. Viral genome could be detected in various organs including brain, lung, spleen, and lymph nodes by re...
Article
To understand the pathology of natural cases of E. coli pathotypes infection in bovine calves, 45 cases of bovine calves, below one month of age, died due to enteritis were studied. Total seventeen cases (37.77%) turned positive for different pathotypes of E. coli by RT-PCR. Out of seventeen positive samples for E. coli, six cases (35.29%) were pos...
Article
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Xenografts are considered as an alternative strategy to restore a critical sized bone defect. The present study was conducted to standardize the technique for seeding and cryopreservation of decellularized bubaline cancellous bone with Guinea pig fetal osteoblast for the development of a composite bone xenograft. The composite bone grafts were prep...
Chapter
In the present study, protocols for decellularizing the buffalo rumen were optimized. The rumen was made acellular using ionic detergents (sodium deoxycholate), nonionic detergents (Triton X-100 and Tween 20), zwitterionic detergents (tri (n-butyl) phosphate), and enzymes (trypsin) in 0.5% and 1% concentration. Treatment with detergents was done up...
Article
Rabies is a zoonotic viral disease with inevitably fatal outcome. Toll-like receptor 3 (TLR3) could sense dsRNA viral infections, and implicated in pathogenesis of rabies and Negri bodies (NBs) formation. Present study was undertaken to elucidate the role of TLR3 in pathogenesis, NBs formation, and therapeutic potential of blocking TLR3/dsRNA inter...
Preprint
African swine fever (ASF) was first reported in 1921, and since then has posed a major threat to the world pig industry and still remains a major challenge as there is no vaccine or therapy available. In May 2020, the first incidence of ASF was recorded in India, followed by a number of outbreaks in the north eastern part of India. In this study, w...
Preprint
Full-text available
We report patho-morphological and virological characterization of SARS-CoV-2 in naturally infected, free ranging Indian Leopard (Panthera pardus fusca). Whole genome sequence analysis confirmed infection of Delta variant of SARS-CoV-2, possibly spill over from humans, but the case was detected when infection level had dropped significantly in human...
Article
Bacterial septicemia causes huge economic losses in the poultry industry and there is no systematic research available in India on the connection of various pathogens associated with septicemia. The present molecular epidemiological study was conducted to investigate the association of different bacterial and immunosuppressive viral pathogens in se...
Article
Background and objective: The wound healing potential of canine bone marrow-derived mesenchymal stem cells (BMSCs) was evaluated in the excisional wound of streptozotocin-induced diabetic rats. Methods: Xenogenic BMSCs were collected aseptically from the iliac crest of healthy canine donors under general anaesthesia. Full-thickness experimental wo...
Article
Bovine brucellosis, predominantly caused by Brucella abortus is one of the most neglected zoonotic diseases causing severe economic losses in the dairy industry. The early and precise diagnosis of the disease is required to reduce the transmission of infection in humans as well as animals. In the current study, a rapid and novel isothermal amplific...
Article
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The smallest polycistronic dsRNA segment-10 (S10) of bluetongue virus (BTV) encodes NS3/3A and putative NS5. The S10 sequence data of 46 Indian BTV field isolates obtained between 1985 and 2011 were determined and compared with the cognate sequences of global BTV strains. The largest ORF on S10 encodes NS3 (229 aa) and an amino-terminal truncated f...
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Bovine abortion is economically one of the most devastating problems faced by dairy farmers. Apart from non-infectious causes, several infectious pathogens are responsible for abortions, which sometimes manifests as abortion storms. Vaccine against several pathogens is available, in spite of that, abortions cause huge economic losses for the dairy...
Article
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Squamous cell carcinoma is one of the most common malignant epithelial skin tumours of all domestic animals. A six-year-old male Spitz dog was presented to District Veterinary Centre, Kannur, Kerala for treatment with history of inappetance, lameness and a mass entirely covering the dorsal surface of the paw of left forelimb. Grossly, nodular ulcer...
Preprint
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Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/ CRISPR associated protein (cas) are now being accepted as a highly specific method of gene editing. Among many other applications, CRISPR/cas has an immense potential to be used as antivirals. In this study, we successfully demonstrated CRISPR/Cas9 mediated inhibition of Bovine Her...
Article
The widely used serodiagnostic test (RBPT, CFT, I-ELISA and FPA) for diagnosis of brucellosis cannot detect vertically infected or carrier animals that are seronegative, a persistent source of infection to other susceptible animals in the herd. For reducing the transmission of disease within the herd, these animals must be detected using a rapid, s...
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Mastitis (intramammary inflammation) caused by infectious pathogens is still considered a devastating condition of dairy animals affecting animal welfare as well as economically incurring huge losses to the dairy industry by means of decreased production performance and increased culling rates. Bovine mastitis is the inflammation of the mammary gla...
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Introduction: Bluetongue disease is an economically important viral disease of livestock caused by bluetongue virus (BTV) having multiple serotypes. It belongs to the genus Orbivirus of family Reoviridae and subfamily Sedoreovirinae. The genome of BTV is 10 segmented dsRNA that codes for 7 structural and 4 nonstructural proteins, of which VP2 was...
Article
The SARS-CoV-2/ COVID-19 pandemic has spread across the globe and affected millions of individuals as of the efficient virus transmission potential mediated via multiple virus shedding routes. The presence of SARS-CoV-2 in the stool samples and its prolonged shedding in environmental compartments like sewage and wastewater signifies a potential thr...
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SARS-CoV-2, which causes coronavirus disease 2019 (COVID-19), is suspected to have been first contracted via animal-–human interactions; it has further spread across the world by efficient human-to-human transmission. Recent reports of COVID-19 in companion animals (dogs and cats) and wild carnivores such as tigers have created a dilemma regarding...
Article
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Bluetongue (BT) is an economically important, non-contagious viral disease of domestic and wild ruminants. Bluetongue is caused by bluetongue virus (BTV) and it belongs to the genus Orbivirus and family Reoviridae. BTV is transmitted by Culicoides midges and causes clinical disease in sheep, white-tailed deer, pronghorn antelope, bighorn sheep, and...
Article
In recent decades, several new diseases have emerged in different geographical areas, with pathogens including Ebola virus, Zika virus, Nipah virus, and coronaviruses (CoVs). Recently, a new type of viral infection emerged in Wuhan City, China, and initial genomic sequencing data of this virus do not match with previously sequenced CoVs, suggesting...
Article
Coronavirus Disease 2019 (COVID-19), caused by SARS-CoV-2 (Severe Acute Respiratory Syndrome - Coronavirus-2 of the family Coronaviridae, appeared in China in December 2019. This disease was declared as posing Public Health International Emergency by World Health Organization on January 30, 2020, attained the status of a very high-risk category on...
Article
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Septicemia in chicken is one of the main causes of death and leads to significant financial losses for the poultry farmers. There is very scarce information available in India on its pathology in chicken. Many bacterial pathogens are known to cause septicemia, either as primary agents or secondary agents, in association with other bacteria or virus...
Article
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Hepatoprotective effects of many antioxidants have been attributed with its uncertain bioavailability at the target cell. Research in drug delivery today is to design a nano-carrier system to deliver drugs at target cell with desired dose and duration. In this study, sodium alginate coated catechin nanoparticle (SACC-NP) carrier system was evaluate...
Preprint
In the past decades, several new diseases have emerged in new geographical areas, with pathogens including Ebola, Zika, Nipah, and coronaviruses (CoVs). Recently, a new type of viral infection has emerged in Wuhan City, China, and initial genomic sequencing data of this virus does not match with previously sequenced CoVs, suggesting a novel CoV str...
Preprint
In the past decades, several new diseases have emerged in new geographical areas, with pathogens including Ebola, Zika, Nipah, and coronaviruses (CoV). Recently, a new type of viral infection has emerged in Wuhan City, China, and initial genomic sequencing data of this virus does not match with previously sequenced CoVs, suggesting a novel CoV stra...
Article
An epidemiological study was conducted for investigation of bovine coronavirus by antigen Enzyme-linked immunosorbent assay kit in dairy calves from Central India (Madhya Pradesh and Chhattisgarh states) and North India (Uttar Pradesh state). Different epidemiological parameters like age, parity, colour and consistency of faecal materials and seaso...
Article
Full-text available
Brucellosis is a common animal disease in India and many other neighboring countries and due to the non-culling policy of carrier cows, the problem is an ever-spreading zoonosis. To find the treatment options, the present study was undertaken to determine the antimicrobial sensitivity of 42 Brucella strains isolated from different animals to antibi...
Article
Bovine rotavirus (BRoV) and bovine coronavirus (BCoV) are major enteric viral pathogens responsible for calve diarrhoea. They are widespread both in dairy and beef cattle throughout the world and causing huge economic losses. The diagnosis of these agents is very difficult due to non-specific nature of lesions and the involvement of some intrinsic...
Article
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Various internal and external factors negatively affect the homeostatic equilibrium of organisms at the molecular to the whole-body level, inducing the so-called state of stress. Stress affects an organism’s welfare status and induces energy-consuming mechanisms to combat the subsequent ill effects; thus, the individual may be immunocompromised, ma...
Article
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Bluetongue (BT) is an infectious, non-contagious, insect-transmitted viral disease of sheep, other domestic, and wild ruminants caused by bluetongue virus (BTV), a prototype species of the genus Orbivirus of the family Reoviridae. The study was designed to determine the hematological and biochemical alterations and acute phase protein response in B...
Article
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Autophagy (self-eating) is a conserved cellular degradation process that plays important roles in maintaining homeostasis and preventing nutritional, metabolic, and infection-mediated stresses. Autophagy dysfunction can have various pathological consequences, including tumor progression, pathogen hyper-virulence, and neurodegeneration. This review...
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For segmented viruses, rapid genomic and phenotypic changes can occur through the process of reassortment, whereby co-infecting strains exchange entire segments creating novel progeny virus genotypes. However, for many viruses with segmented genomes, this process and its effect on transmission dynamics remain poorly understood. Here, we assessed th...
Article
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An epidemiological study was conducted for detection of rotavirus by antigen ELISA kit in dairy calves from Central India (Madhya Pradesh and Chhattisgarh states) and North India (Uttar Pradesh state). Different epidemiological parameters like age, sex, parity, colour and consistency of faecal materials and seasonal changes in a year were recorded....
Article
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Aim: This study was conducted to know the genetic variability of rabies viruses (RVs) from wild animals in India. Materials and Methods: A total of 20 rabies suspected brain samples of wild animals from different states of India were included in the study. The samples were subjected for direct fluorescent antibody test (dFAT), reverse transcriptio...
Article
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An epidemiological study was conducted along with pathological, immunohistochemistry (IHC) of capsid antigen and molecular detection of jaagsiekte sheep retrovirus (JSRV) causing ovine pulmonary adenocarcinoma (OPA) in sheep and goats population from three states of India viz. Delhi, Gannavaram (Andhra Pradesh), and Bareilly (Uttar Pradesh). A tota...
Article
Rabies is a neglected viral zoonotic disease affecting humans, domestic and wild animals and is endemic in most parts of the India. Dog mediated rabies is more predominant than other forms of rabies and molecular epidemiology is poorly understood in both reservoir and susceptible hosts. In the present study, a total of 140 rabies suspected brain sa...
Article
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Aim The aim of this study was to develop Polymerase Spiral reaction (PSR) for rapid, sensitive and specific detection of Brucella spp. Methods and Results PSR assay was developed using specifically designed primers targeting the conserved multicopy IS711 gene of Brucella spp. The assay could be performed within 60 min at an isothermal temperature...
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We describe here the intestinal and extra-intestinal spread of the species A rotavirus (RV-A) and associated lesions thereof in Swiss albino suckling mice pups, inoculated with a bovine-origin RV-A strain. In total, 35 suckling pups were used, wherein 20 pups received cell culture isolated RV-A @ 160 μL (TCID50/ml, 5 × 106.5) per pup [oral 80 μL an...
Article
Foot-and-mouth disease (FMD) is a highly contagious and economically important, transboundary viral disease of cloven-hoofed animals. It is known that an asymptomatic, persistent FMD virus (FMDV) infection may occur subsequent to acute or subclinical FMDV infection in adult ruminants. However, virus persistence in young calves has not been studied....
Article
To understand the pathology of natural cases of rotavirus (RVA) in bovine calves, a total of 40 cases below 6 months died due to diarrhoea were studied, out of which 7 cases (17.5%) turned positive for RVA by RT-PCR. Histopathology of small intestine showed loss of villous enterocytes, blunting and fusion of villi, elongation of crypts and mononucl...
Article
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Babesia gibsoni is the common cause of canine babesiosis in India. Its diagnosis by conventional diagnostic methods result in false negative condition, particularly in those cases in which parasitemia is low. Therefore the present study employed IFAT and PCR along with microscopic examination to overcome this situation. Microscopic examination of G...