Kamalesh Prasad

Kamalesh Prasad
Central Salt and Marine Chemicals Research Institute | CSMCRI · Marine Biotechnology and Ecology Research Area (CSMCRI)

PhD

About

150
Publications
32,513
Reads
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3,812
Citations
Citations since 2016
61 Research Items
2664 Citations
20162017201820192020202120220100200300400500
20162017201820192020202120220100200300400500
20162017201820192020202120220100200300400500
20162017201820192020202120220100200300400500
Additional affiliations
January 2008 - January 2009
Kagoshima University
Position
  • Guest Scientist

Publications

Publications (150)
Article
Full-text available
Among various protein-containing biomass wastes, waste animal wool, poultry feather, and human hair are considered one of the most important renewable sources of keratin. Animal wool and human hair are utilized for the production of several products. However, the substantial quantity of short fibers that are inappropriate for spinning and being unu...
Article
Full-text available
In India, Kappaphycus alvarezii is the only alga being cultivated commercially for kappa carrageenan production and also being utilized for the socioeconomic upliftment of coastal rural population in India in the form of their cultivation and marketing. The production has been substantially increased from 21 dry tonnes to 1490 dry tonnes with incre...
Article
Full-text available
The field of Ionic liquids (ILs) and deep eutectic solvents (DESs) is continuously expanding due to their exceptional unique properties and highly tunable nature, which finds applications in broad areas of modern science. Considering numerous possible IL and DES combinations prepared with active pharmaceutical ingredients (APIs), they find applicat...
Article
In India, Kappaphycus alvarezii is the only alga being cultivated commercially for kappa carrageenan production and also being utilized for the socioeconomic upliftment of coastal rural population in India in the form of their cultivation and marketing. The production has been substantially increased from 21 dry tonnes to 1490 dry tonnes with incre...
Article
Full-text available
Seaweeds, due to their diverse product profile, are in huge demand globally and therefore, to cater feedstock requirements, farming is pivotal. Gracilaria dura from Indian waters due to its quality agar has recently gained industry attention. This species has shown considerable phenotypic differences in wild and cultivated clones after uninterrupte...
Article
Agarose is a seaweed-based polysaccharide and is widely used for the separation of nucleic acids in molecular biology. Cross-linked agarose beads are also used as solid-phase matrices in size exclusion chromatography for the separation of proteins. To find the application of agarose for the separation of oil/water emulsion and protein, herein hydro...
Article
Due to the increasing trend to use ionic liquids (ILs) for number of applications, it is of utmost importance to ensure non toxicity of the solvent systems which may contaminate the processed products. The reported toxicity of several imidazolium based ionic liquids posed a need to develop bio based ILs for various applications which are due to the...
Article
Hydrogels are considered as practical and proficient materials in adsorption and removal of soluble lethal molecules from aqueous system. They are also rapid-decomposable and economical materials besides their diverse preventive claims. In current study, Cinnamaldehyde (C), a natural defensive compound and Chitosan (Ch), natural occurring bio-macro...
Preprint
Full-text available
Among protein-containing biomass waste, waste animal wool, poultry feather, and human hair are considered as one of the most important renewable sources of keratin. Although waste animal wool (WAW) and human hair (WHH) are used for the production of number of products but the substantial quantity of short fibers not suitable for spinning and being...
Preprint
Full-text available
Among protein-containing biomass wastes, waste animal wool, poultry feather, and human hair are considered as one of the most important renewable sources of keratin. Although waste animal wool (WAW) and human hair (WHH) are used for the production of the number of products but the substantial quantity of short fibers not suitable for spinning and b...
Article
Full-text available
Several emerging therapeutic applications of agar and its derivatives, make it one of the sought-after hydrocolloids, commanding the highest prize in global market. Nevertheless, farming of local agarophyte species was commercially successful thereby aptly supporting domestic demand coupled with natural harvesting. Several investigations addressed...
Preprint
Melanins have multidimensional applications in medical and biomaterial science and thereby shooting up its demand. Conventional extraction techniques has many disadvantages like poor extraction efficiency, use of toxic solvents (acid and base), time-consuming, and consequent higher cost of the final product. To tackle this problem, an innovative io...
Article
Full-text available
Among the various natural polymers, polysaccharides are one of the oldest biopolymers present on the Earth. They play a very crucial role in the survival of both animals and plants. Due to the presence of hydroxyl functional groups in most of the polysaccharides, it is easy to prepare their chemical derivatives. Several polysaccharide derivatives a...
Article
Full-text available
The red alga Gracilaria dura is economically important due to its high-quality agarose. Previous studies with wild populations reported the existence of specific differences in functional traits as well as agar characteristics among life cycle stages. In farmed populations, such differences can be exploited for commercial gains. For that, the varia...
Article
Full-text available
Large-scale production of graphene sheets by liquid-phase exfoliation of graphite is a challenging task from a sustainability point of view. Certain bio-derived solvents were found to exfoliate graphite to produce single-layered graphene sheets but the high cost of the solvent is always a deterring factor towards upscaling of the process. Herein, K...
Article
From the very first day of the introduction of deep eutectic solvents (DESs) as an alternative to the ionic liquids (ILs), it has been claimed that both the solvents enjoy similar physiochemical properties. The claim was found not to be correct when the dissolution phenomenon of DNA in the ILs and DESs consisting of a few selected similar cations a...
Article
Blue biorefinery is the integrate production of renewable chemicals, fuels, functional materials and marketable commodities utilising biomass of marine origin. Global climate issues, ever-increasing population and depletion of fossil resources...
Article
Full-text available
This study reports targetable micelles developed after covalent functionalization of α-tocopheryl polyethylene glycol succinate (TPGS) with amino phenylboronic acid (APBA). Nuclear magnetic resonance (NMR) and infrared (IR) spectroscopic results showed successful attachment of APBA to the hydrophilic segment of TPGS. Dynamic light scattering and sm...
Article
Suitability of seven different bio-based ionic liquids (Bio-ILs) namely choline fumarate (Ch-Fu), choline adipate (Ch-Adi), choline caproate (Ch-Cap), choline caprylate (Ch-Capl), choline capriate (Ch-Capr) and choline laurate (Ch-Lau) to selectively coagulate κ-carrageenan from Kappaphycus alvarezii seaweed extract obtained with both water and alk...
Article
Suitability of seven different bio-based ionic liquids (Bio-ILs) namely choline fumarate (Ch-Fu), choline adipate (Ch-Adi), choline caproate (Ch-Cap), choline caprylate (Ch-Capl), choline capriate (Ch-Capr) and choline laurate (Ch-Lau) to selectively coagulate κ-carrageenan from Kappaphycus alvarezii seaweed extract obtained with both water and alk...
Article
Full-text available
Throughout numerous research works on biomacromolecules, several breakthrough innovations have occurred in the field of biomacromolecule processing. Remarkable improvements have been made so far to address the problems associated with biomacromolecule processing technologies in terms of enhancing the efficiency of the processes. Green technology br...
Article
Polyhydroxyalkanoates (PHAs) have a wide range of applicability due to their biodegradable and biocompatible characteristics. This makes them suitable for their commercialization as an alternative to petroleum-based synthetic plastics. Solvents such as chloroform are reported to solubilize polyhydroxyalkanoates. Chloroform being hazardous for both...
Article
Slow release nano‐fertilizers is of interest for reducing soil nutrient losses and prevent land degradation due to use of fertilizers. Chitosan‐nanoparticle (CN) was thus prepared following polymerization of chitosan with methacrylic‐acid and later incorporated with potassium (CNK). CN as well as CNK were characterized using Fourier‐transform infra...
Article
Antibodies present in mammal’s serum are of high relevance for therapeutic purposes, particularly in passive immunization and in the treatment of some chronic diseases. However, their recovery with high purity...
Article
Four different ionic liquids were synthesized, characterized and used with polypropylene glycol for the formation of aqueous biphasic systems and were used to selectively separate ε-PL from culture broth. It was observed that the partition of ε-PL to the IL-rich phase was achieved, and with pure ε-PL, the extraction efficiency was found to be 99.92...
Article
This study describes biosourced binary materials that synergize the attributes of guar gum, a vegetal galactomannan, with the ones of deep eutectic solvents (DES). The preparation of a library of DES, stemming from the H-bonding complexation of renewable molecules such as choline chloride (ChCl) with urea, di or triacids, polyols, or carbohydrates...
Article
Polysaccharides of marine origin, for example, agarose (Ag) and alginic acid (Alg)‐based new nano‐size monoamido amino acid derivatives are synthesized and their protein‐like characteristics are evaluated. Two new agarose‐based nanosize amino acid derivatives are obtained by partial amidation of agarose succinate half‐ester (Ag‐SA; DS 0.95) employi...
Article
Metallized DNA is an exciting functional material having widespread utility towards multifunctional applications. However, conventional DNA metallization processes are time consuming, multi-steps, and most importantly helicity of DNA is destroyed during metallization process. Herein, an ecofriendly and rapid approach has been demonstrated to metall...
Article
The extraordinary left handed conformation of DNA known as Z-DNA has attracted the attention of structural biologists due to its characteristic features such as its possible role in regulation of gene expression and genetic instability. There are number of physical parameters which can induce the conformational transformation of double helical B-DN...
Article
The abundance and unique characteristics of keratin containing natural protein fibres such as wool and silk envisage their resourcing as alternative to certain petroleum based polymers. Among keratin containing resources, human hair is one of the most abundant keratin rich natural fibre and is one of the largest waste materials produced by the huma...
Article
Full-text available
Gracilaria debilis (Forsskål) Børgesen, an economically important red alga, was cultivated for two years by a floating bamboo raft method from Nov-2014 - Oct-2016 along the southeastern coast of India in order to evaluate possibilities for its year-round cultivation. This was achieved by analyzing biomass yield, growth rate and agar properties from...
Article
Solvothermal carbon (STC) has emerged as exciting functional materials for diverse applications. However, designing STC with tunable properties (for instance shape, chemical functionalities, surface acidity, and etc.) for on-demand application...
Article
Dissolution and processing of biopolymers perhaps is one of the oldest chemical processes in the world, and there are several breakthrough inventions took place in this area. However, with the advent of technological interventions, the number of improvements in the processing technologies of the biopolymers has been made to address the efficiency a...
Article
Herein, alkyl chain dependent self-assembly of choline carboxylate ionic liquids (ILs) and their effect on micellar characteristics of a star block copolymer (Tetronic®T1304) are reported. The ILs showed good aqueous solubility (low Kraft point) and formed spherical micelles. Small angle neutron scattering (SANS) analyses revealed a reduction in th...
Article
Packaging of structurally sensitive biomolecules such as proteins, peptides and DNA in non-aqueous media at ambient conditions with chemical and structural stability is important to explore the potential of such biomacromolecules as substrate for functional biomaterial design and for biotechnological applications. In this perspective, solubility, c...
Patent
The present invention relates to the process for the scalable production of Fe3O4/Fe, Sn & Zn doped graphene nanosheets from a naturally abundant seaweed resources such as Sargassum tenerrimum, Sargassum wighti, Ulva faciata, Ulva lactuca and Kappaphycus alvarezii. The granules remained after the recovery of liquid juice from the fresh seaweeds wer...
Article
Cultivation of the wild-type red strain and green natural mutant strains of Gracilaria debilis (Forsskål) Børgesen (RD and GD) and Gracilaria edulis (Gmelin) Silva (RE and GE) was attempted by raft method from August, 2014 to May, 2015 at Thonithurai, southeastern coast of India, for 180 days in order to evaluate color stability, growth rates, pigm...
Technical Report
Full-text available
Sustainable and low-cost reusable water recovery process from industrial wastewater may have potential positive impact towards potable water crisis in several industrial processes. Quite a number of approaches such as chemical processes, biological treatment and high-pressure membrane based processes have been developed to address this crucial issu...
Article
Full-text available
Sustainable and low-cost reusable water recovery process from industrial wastewater may have potential positive impact towards potable water crisis in several industrial processes. Quite a number of approaches such as chemical processes, biological treatment and high-pressure membrane-based processes have been developed to address this crucial issu...
Article
Ion gels and self-healing gels prepared using ionic liquids (ILs) and deep eutectic solvents (DESs) have been largely investigated in the past years due to their remarkable applications in different research areas. Herewith we provide an overview on the ILs and DESs used for the preparation of ion gels, highlight the preparation and physicochemical...
Chapter
Availability of biomass is the key to the implementation of bioresources for the production of fuel, chemicals, fertilizer and materials. Besides focusing on waste bioresources, it would be essential to identify the potential of relatively xunexploited biomass such as seaweed. Several approaches have been devoted to adding value to algal feedstocks...
Article
Full-text available
Properties of cellulose extracted from Valoniopsis pachynema, which is a commercially unexploited green seaweed found in Indian waters was evaluated for its microcrystalline properties and paper forming abilities. The crude cellulose obtained from the seaweed was found to have a high crystallinity (95%) equivalent to commercially available microcry...
Article
Two new nano-sized fluorescent 6-amino agarose naphthalic acid half ester derivatives were synthesized (ca.60% yields) employing 1,8- and 2,3-naphthalic acid anhydrides (1,8-AANE, and 2,3-AANE respectively). These large nano molecular frameworks (DLS 3 & 100 nm, and 3 & 152 nm respectively) contains amino, naphthalate half-ester carboxyl groups at...
Article
The amphiphilic nature of many pharmaceutical active ingredients often makes them difficult to solubilise and leads to significant wastage through non-optimal dosage. In this study it is shown that highly concentrated liquid formulations can be produced from pharmaceutical active ingredients which either contain a strong hydrogen bonding functional...
Article
Herein we present a sustainable and cost effective approach for the preparation of functionalized graphene nanosheets (GNs) directly from seaweed and deep eutectic solvents (DES). The seaweed granules remained after the recovery of juice from fresh brown seaweed, Sargassum tenerrimum was utilized as a raw material and DES generated by the complexat...
Article
Packaging as well as preservation of DNA in non-aqueous media at ambient conditions for long durations is an important research endeavor considering the biomacromolecule as nanoscale substrate for functional biomaterial design and for biotechnological applications. In this perspective, the present work reports both very high concentration dissoluti...
Article
Full-text available
Cultivation of the wild-type red strain and green mutant strains of Gracilaria debilis (Forsskål) Børgesen (RD and GD) and Gracilaria edulis (Gmelin) Silva (RE and GE) was attempted by raft method from August, 2014 to May, 2015 at Thonithurai, South eastern coast of India for 180 days in order to evaluate color stability, growth rates, pigment comp...
Article
In the past decade, remarkable advances on the production and use of antibodies as therapeutic drugs and in research/diagnostic purposes have lead to their recognition as value-added proteins. These biopharmaceuticals have become increasingly important, reinforcing the current demand on the development of more benign, scalable and cost-effective te...
Article
In a rapid one-step method protein-mimicking large agarose amino acid framework (AAE; GPC 156.7 kDa) was conjugated with polyethylene glycol (PEG 9 kDa) affording nano-sized PEGylated amphoteric agarose (PEG-AAE; <10 nm; DLS) containing amino, carboxyl and ester groups [overall degree of substitution (DS) 0.91]. The PEG groups were at the residual...
Article
Self-polymerization of optimized amount of 2-hydroxyethylmethacrylate (HEMA) in a natural deep eutectic solvent (NADES) is obtained by the complexation between choline chloride and fructose (ChCl:Fruc. 2:1) in the presence of indomethacin, an anti-inflammatory drug resulted formation of a drug-immobilized ion gel. The ion gel thus obtained is found...
Article
Herewith we have utilized the ability of a choline based deep eutectic solvent (DES) to dissolve DNA and disperse graphene for the synthesis of a Fe3O4 functionalized DNA-N-doped graphene hybrid material. N-doped graphene prepared from graphene oxide and melamine by solar radiation was found to envelop the Fe3O4 functionalized DNA in a sandwich fas...
Article
A novel natural deep eutectic solvent (NADES) was prepared by the endothermic complexation of choline chloride (hydrogen bond acceptor) and orcinol (hydrogen bond donor) at room temperature. Among N-isopropyl acrylamide (NIPAM), vinyl acetate (VA) and 2-hydroxyethyl methacrylate (HEMA), only HEMA was found to be self-polymerized in the above NADES...
Article
Deep eutectic solvents (DESs) obtained by the complexation of choline chloride (ChoCl) as hydrogen bond acceptor and hydrogen bond donors such as ethylene glycol (ChoCl-EG 1:2) and glycerol (ChoCl-Gly 1:2) were used as media for the formation of AgCl crystals. Although formation of AgCl crystals was observed in both the solvents but the rate of for...
Article
Among four different biomass derived green and sustainable solvents namely levulinic acid (LA), ethyl lactate (EL), γ-valerolactone (GVL) and formic acid (FA) only LA was found to exfoliate graphite to single and few layered graphene sheets. During exfoliation, the formation of LA crystals embedded with single layered graphene sheets was observed....
Article
Herein we report a facile method for the scalable production of Fe3O4/Fe doped graphene nanosheets (Fe3O4/Fe-GN) from a naturally abundant seaweed resource. The granules remained after the recovery of liquid juice from a fresh brown seaweed, Sargassum tenerrimum was utilized as a raw material and a deep eutectic solvent (DES) generated by the compl...
Article
2.5% w/w DNA (Salmon testes) was solubilized in a bio-deep eutectic solvent [(bio-DES), obtained by the complexation of choline chloride and ethylene glycol at 1:2 molar ratio] containing 1% w/w of silver chloride (AgCl) to yield a AgCl decorated DNA based hybrid material. Concentration dependent formation of AgCl crystals in the DES was observed a...
Technical Report
Researchers have synthesized green organic solvents that could be used to concentrate low-abundance biomolecules such as proteins and DNA from a mixture of biomolecules, facilitating further purification and extraction1. Their technique is potentially attractive for extracting pure proteins and DNA for clinical research and the food industry. Beca...
Article
Full-text available
Present study explores potential of green and sustainable deep eutectic solvents (DESs) as an alternative energy source for concentration of protein (>6 times) and DNA (>3 times) using forward osmosis (FO). The distinct colligative properties like high osmotic pressure (Π >300 atm) and low water activity (aw, ≤0.1) of the DESs make them ideal draw...
Article
Three different deep eutectic solvents (DESs) prepared by the complexation of choline chloride with urea, ethylene glycol and glycerol along with their hydrated counterparts were used for the selective extraction of κ-carrageenan from Kappaphycus alvarezii. Upon comparison of the quality of the polysaccharide with the one obtained using water as ex...
Article
Three different imidazolium based ionic liquids namely 1-butyl-3-methylimidazolium hexafluorophosphate [Bmim][PF6], 1-methyl-3-octylimidazolium tetrafluoroborate [Omim][BF4] and 1-butyl-3-methylimidazolium bis (trifluoromethylsulfonyl) imide [Bmim][NTf2] were used to extract plant growth regulators (PGRs) present in the sap (K-sap) obtained by the...
Article
Different neoteric solvents (both ionic liquids and deep eutectic solvents) were used for the dispersion and preparation of bio-nanocomposite of spider silk fibres (Crossopriza lyoni). Among these solvents, hydrated tetrabutylammonium hydroxide was found to disperse 7.5 mg mL-1 of spider silk with nano-scale structural distribution at room temperat...