Kalliope K Papadopoulou

Kalliope K Papadopoulou
University of Thessaly | UTH · Τμήμα Βιοχημείας και Βιοτεχνολογίας

About

114
Publications
28,244
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3,545
Citations
Additional affiliations
October 2005 - present
University of Thessaly
September 2000 - October 2005
June 1997 - August 1999
John Innes Centre
Education
September 1986 - June 1990

Publications

Publications (114)
Preprint
Full-text available
A growing body of evidence suggests that RNA interference (RNAi) plays a pivotal role in the communication between plants and pathogenic fungi, where a bi-directional cross-kingdom RNAi is established to the advantage of either the host or the pathogen. Similar mechanisms acting during plant association with non-pathogenic symbiotic microorganisms...
Article
Full-text available
Background: Grapevine trunk diseases (GTDs) is a disease complex caused by wood pathogenic fungi belonging to genera like Phaeomoniella, Phaeoacremonium, Fomitiporia, Eutypa and members of the family Botryosphaeriaceae. However, the co-occurrence of these fungi in symptomatic and asymptomatic vines at equivalent abundances has questioned their rol...
Article
Sand dunes of the Mediterranean region constitute drought-stressed, low-fertility ecosystems. Arbuscular mycorrhizal fungi (AMF) are regarded as key components of their biota, that contribute to plant host adaptation and fitness. However, AMF community assembly rules in the roots of the psammophilous plants of coastal sand dunes have not been inves...
Article
Arbuscular mycorrhizal fungi (AMF) are soilborne microorganisms that establish a mutualistic symbiotic association with most of land plants. To investigate the effects of AMF symbiosis under different water status conditions, we grew AMF-inoculated and non-inoculated tomato plants in the greenhouse under two irrigation regimes, 70% and 30% of growt...
Article
The glycogen synthase kinases 3 family (GSK3s/SKs; serine/threonine protein kinases) is conserved throughout eukaryotic evolution from yeast to plants and mammals. We studied a plant SK kinase from Lotus japonicus (LjSK1), previously implicated in nodule development, by enzyme kinetics and mutagenesis studies to compare it to mammalian homologues....
Article
Full-text available
Plants are a rich source of specialized metabolites with a broad range of bioactivities and many applications in human daily life. Over the past decades significant progress has been made in identifying many such metabolites in different plant species and in elucidating their biosynthetic pathways. However, the biological roles of plant specialized...
Article
The effect of copper (Cu-NPs, CuO-NPs), silver (Ag-NPs) and zinc oxide (ZnO-NPs) nanoparticles (NPs) on plant growth, physiological properties of tomato plants and their symbiotic relationships with the endophytic Fusarium solani FsK strain was investigated. Fungitoxicity tests revealed that the FsK strain was significantly more sensitive to Cu-NPs...
Chapter
In nature, plants are continuously challenged by biotic stressors such as pathogenic microorganisms and herbivorous pests that often cause important yield reductions in crops. To address global food security issues but also consumer demands for healthier food production, a series of environmentally friendly crop protection strategies need to be emp...
Article
Mutualistic relationships of legume plants with, either bacteria (like rhizobia) or fungi (like arbuscular mycorrhizal fungi), have been investigated intensively, usually as bi-partite interactions. However, diverse symbiotic interactions take place simultaneously or sequentially under field conditions. Their collective, but not additive, contribut...
Book
Full-text available
The term “endophytes” usually refers to communities of microorganisms, typically bacteria or fungi, which colonize the internal portion of plants, mainly vascular tissues, interacting with the host in either beneficial or neutral mode. The scientific community customarily classifies endophytes into three main subsets based on facultative, obligate...
Article
Full-text available
•This work describes a protocol for hairy root transformation of the medicinal crop legume fenugreek (Trigonella foenum-graecum L.). Hairy root plant transformation mediated by Agrobacterium rhizogenes is an established method for the rapid genetic transformation of various dicotyledonous plants. We have adapted a hairy root transformation protocol...
Article
Full-text available
Glycosylation is one of the most prevalent molecular modifications in nature. Single or multiple sugars can decorate a wide range of acceptors from proteins to lipids, cell wall glycans and small molecules, dramatically affecting their activity. Here, we discovered that by ‘hijacking’ an enzyme of the cellulose synthesis machinery involved in cell...
Article
Full-text available
Cultivation of olive trees covers large coastal areas of land in Mediterranean regions, many of them characterized by low soil fertility and exposed to salinity and seasonal drought. In this frame, we developed mixed community inocula of arbuscular mycorrhizal fungi (AMF) derived from the extreme, seasonally arid environments of six Mediterranean s...
Article
The development of genetic transformation methods is critical for enabling the thorough characterization of an organism and is a key step in exploiting any species as a platform for synthetic biology and metabolic engineering approaches. In this work we describe the development of an Agrobacterium rhizogenes-mediated hairy root transformation proto...
Article
Full-text available
Exogenous RNA interference (exo-RNAi) is a powerful transgene-free tool in modern crop improvement and protection platforms. In exo-RNAi approaches, double stranded RNAs (dsRNAs) or short-interfering RNAs (siRNAs) are externally applied in plants in order to selectively trigger degradation of target mRNAs. Yet, the applied dsRNAs may also trigger u...
Poster
Full-text available
So far, biotechnological RNAi approaches in plants have been based on RNAi-inducing transgenes and/or transgenic plants. Here, we describe novel methods for the exogenous delivery in plants of RNA molecules having the potential to trigger RNAi in a transgene-free manner.
Article
Full-text available
Context: Satureja khuzistanica Jamzad. (Lamiaceae), is known for its antifungal and antioxidant compounds, especially rosmarinic acid (RA). Objective: The study examines the effect of elicitors on RA production and phytochemical properties of S. khuzistanica. Materials and methods: In vitro plants were treated with methyl jasmonate (MeJA) and multi...
Article
Legumes interact with a wide range of microbes in their root system, ranging from beneficial symbionts to pathogens. Symbiotic rhizobia and arbuscular mycorrhizal glomeromycetes trigger a so‐called common symbiotic signalling pathway (CSSP), including the induction of nuclear calcium spiking in the root epidermis. By combining gene expression analy...
Preprint
Full-text available
Legumes interact with a wide range of microbes in their root system, ranging from beneficial symbionts to pathogens. Symbiotic rhizobia and arbuscular mycorrhizal glomeromycetes trigger a so-called common symbiotic signalling pathway (CSSP), including the induction of nuclear calcium spiking in the root epidermis. In our study, the recognition of a...
Article
Full-text available
Satureja khuzistanica is a medicinal herb endemic to Iran which can serve as a source of rosmarinic acid (RA). In the present study, the effect of different concentrations of methyl jasmonate (MeJA) and multi-walled carbon nanotubes (MWCNTs) on the rosmarinic acid accumulation and the expression of genes involved in its biosynthetic pathway was eva...
Article
Plant cellular responses to endophytic filamentous fungi are scarcely reported, with the majority of described colonization processes in plant-fungal interactions referring to either pathogens or true symbionts. Fusarium solani strain K (FsK) is a root endophyte of Solanum lycopersicum, which protects against root and foliar pathogens. Here, we inv...
Article
Full-text available
Eight endophytic bacterial strains (Bacillus spp., Stenotrophomonas maltophilia, and Pseudomonas geniculata) recovered from healthy cultivated tomato (Solanum lycopersicum L.) were screened for their plant growth-promoting potential on tomato plants challenged with Fusarium oxysporum f. sp. lycopersici (FOL) and for their in vitro and in vivo antif...
Article
Full-text available
Glycogen Synthase Kinase/Shaggy‐like kinases (SKs) are a highly conserved family of signaling proteins that participate in many developmental, cell‐differentiation, and metabolic signaling pathways in plants and animals. Here, we investigate the involvement of SKs in legume nodulation, a process requiring integration of multiple signaling pathways....
Article
Full-text available
Background: Understanding the mechanisms involved in climacteric fruit ripening is key to improve fruit harvest quality and postharvest performance. Kiwifruit (Actinidia deliciosa cv. ‘Hayward’) ripening involves a series of metabolic changes regulated by ethylene. Although 1-methylcyclopropene (1-MCP, inhibitor of ethylene action) or ozone (O3) ex...
Article
Full-text available
Beneficial microorganisms are known to promote plant growth and confer resistance to biotic and abiotic stressors. Soil-borne beneficial microbes in particular have shown potential in protecting plants against pathogens and herbivores via the elicitation of plant responses. In this study, we evaluated the role of Fusarium solani strain K (FsK) in a...
Article
Full-text available
Background: Post-translational modification of receptor proteins is involved in activation and de-activation of signalling systems in plants. Both ubiquitination and deubiquitination have been implicated in plant interactions with pathogens and symbionts. Results: Here we present LjPUB13, a PUB-ARMADILLO repeat E3 ligase that specifically ubiqui...
Article
Full-text available
The potential of FsK, a non-pathogenic endophytic Fusarium solani strain, to be utilized as a biocontrol agent in combination with nine selected fungicides registered in tomato crops in Greece was evaluated. In vitro fungitoxicity tests revealed that FsK was insensitive to doses exceeding 100 μg/mL of thiophanate-methyl, fenhexamid, cyprodinil, bos...
Article
Full-text available
Belowground symbiosis of plants with beneficial microbes is known to confer resistance to aboveground pests such as herbivorous arthropods and pathogens. Similarly, microbe-induced plant responses may also impact natural enemies of pests via the elicitation of plant defense responses and/or alteration of plant quality and growth. Nesidiocoris tenui...
Article
The endophyte Fusarium solani strain FsK may colonize the roots of tomato plants and protect them against fungal pathogens. Here we show that the fungus can alleviate the strong negative effects of water stress on the development and growth of tomato seedlings that were co-cultivated with FsK under in vitro conditions. In a succeeding pot experimen...
Preprint
Post-translational modification of receptor proteins is involved in activation and de-activation of signaling systems in plants. Both ubiquitination and deubiquitination have been implicated in plant interactions with pathogens and symbionts. Here we present LjPUB13 , a PUB-ARMADILLO repeat E3 ligase that specifically ubiquitinates the kinase domai...
Article
Full-text available
Ortho-phenylphenol (OPP) is a fungicide contained in agro-industrial effluents produced by fruit-packaging plants. Within the frame of developing bio-strategies to detoxify these effluents, an OPP-degrading Sphingomonas haloaromaticamans strain was isolated. Proteins/genes with a putative catabolic role and bacterium adaptation mechanisms during OP...
Article
Five endophytic Bacillus spp. isolated from surface-sterilized stem tissues of wild Solanaceous species, i.e. Datura metel, Solanum nigrum and S. elaeagnifolium, were screened for their in vitro and in vivo antagonistic potential against Fusarium oxysporum f. sp. lycopersici (FOL). Pathogen sporulation was totally suppressed by these five strains a...
Article
Plant glutathione peroxidases (Gpx) catalyse the reduction of various peroxides, such as hydrogen peroxide (H2O2), phospholipid hydroperoxides and peroxynitrite, but at the expense of thioredoxins rather than glutathione. A main function of plant Gpxs is the protection of biological membranes by scavenging phospholipid hydroperoxides, but some Gpxs...
Article
Glucosinolates (GSLs) are a highly important group of secondary metabolites in the Caparalles order, both due to their significance in plant-biome interactions and to their chemoprotective properties. This study identified genes involved in all steps of aliphatic and indolic GSL biosynthesis in Eruca sativa, a cultivated plant closely related to Ar...
Article
Glucocorticoids are steroid hormones widely used to control many inflammatory conditions. These effects are primarily attributed to glucocorticoid receptor transrepressional activities but with concomitant receptor transactivation associated with considerable side effects. Accordingly, there is an immediate need for selective glucocorticoid recepto...
Article
A putative endophytic Bacillus cereus str. S42 (KP993206), recovered from surface-sterilised stems of Nicotiana glauca was assessed in vitro and in vivo for its antifungal potential towards Fusarium oxysporum f. sp. lycopersici (FOL). Pathogen sporulation was significantly inhibited by B. cereus str. S42. FOL mycelial growth was reduced using its w...
Article
Soil fumigants constitute a major tool for the control of soil borne fungal plant pathogens in protected crops. Dimethyl disulfide (DMDS) is a novel soil fumigant used either alone or in combination with other fumigants for the control of soil borne pests and diseases. In a commercial greenhouse for tomato production, we evaluated the impact of DMD...
Article
ABSTRACTWe report the identification and characterization of a novel gene, AtHesperin (AtHESP) that codes for a deadenylase in Arabidopsis thaliana. The gene is under circadian clock-gene regulation and has similarity to the mammalian Nocturnin. AtHESPcan efficiently degrade poly(A) substrates exhibiting allosteric kinetics. Size exclusion chromato...
Article
Full-text available
In this study, chemical composition and growth responses of chia plants (Salvia hispanica L.) to inoculation with an arbuscular mycorrhiza (AM, Glomus mosseae, Nicol. & Gerd.) fungal inoculum (namely MC10) under the influence of soil pH were investigated. The experiment project included six treatments, i.e., control-non-arbuscular mycorrhiza fungi...
Article
Full-text available
Despite their importance in many aspects of plant physiology, information about the function of oxidative and, particularly, of nitrosative signalling in fruit biology is limited. This study examined the possible implications of O3 and sodium nitroprusside (SNP) in kiwifruit ripening, and their interacting effects. It also aimed to investigate chan...
Article
Full-text available
Abstract Saponins are widely distributed plant natural products with vast structural and functional diversity. They are typically composed of a hydrophobic aglycone, which is extensively decorated with functional groups prior to the addition of hydrophilic sugar moieties, to result in surface-active amphipathic compounds. The saponins are broadly c...
Article
Four different chemical treatments, GA3, 1‐MCP, essential oils and nano‐Cu, were applied immediately after harvest to Petroselinum crispum (Mill) plants. The efficacy of the above chemicals on shelf life extension of parsley stored at 5 °C and 20 °C was determined by analysing physiological and biochemical factors that determine quality standards o...
Article
Genes for triterpene biosynthetic pathways exist as metabolic gene clusters in oat and Arabidopsis thaliana plants. We characterized the presence of an analogous gene cluster in the model legume Lotus japonicus. In the genomic regions flanking the oxidosqualene cyclase AMY2 gene, genes for two different classes of cytochrome P450 and a gene predict...
Article
Glucosinolates (GSLs) are secondary metabolites found in Brassica species. Upon tissue disruption GSLs are hydrolyzed by myrosinase enzymes to isothiocyanates (ITCs) which are highly toxic to microbes. Therefore the incorporation of fresh Brassicaceae tissues into soil for the control of soil-born plant pathogens is viewed as a biofumigation proces...
Article
Plants produce an array of diverse secondary metabolites with important ecological functions, providing protection against pests, diseases, and abiotic stresses. Secondary metabolites are also a rich source of bioactive compounds for drug and agrochemical development. Despite the importance of these compounds, the metabolic diversity of plants rema...
Article
Suppressive compost provides an environment in which plant disease development is reduced, even in the presence of a pathogen and a susceptible host. Despite the numerous positive reports, its practical application is still limited. The main reason for this is the lack of reliable prediction and quality control tools for evaluation of the level and...