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Kalindu Ramyasoma holds a doctorate in Molecular Entomology from the University of Colombo's Department of Chemistry, Faculty of Science. His main scientific interests include transgenic technology and its applications in the medical and agricultural sectors. His graduate thesis was titled "Engineering RNA interference-based resistance to dengue virus in Aedes aegypti vector mosquitoes."
Background: Genetic modification offers opportunities to introduce artificially created molecular defence mechanisms to vector mosquitoes to counter diseases causing pathogens such as the dengue virus, malaria parasite, and Zika virus. RNA interference is such a molecular defence mechanism that could be used for this purpose to block the transmiss...
Dengue viruses (DENV) are the wildest transmitted arbovirus members of the family Flaviviridae, genus Flavivirus. Dengue viruses are composed of four serotypes, DENV1, 2, 3, and 4, and these viruses can cause dengue fever and dengue haemorrhagic fever or dengue shock syndrome, when infecting humans. RNA interference (RNAi) is a self-defence mechani...
The development of first ever transgenic animal in Sri Lanka and dengue transmission resistant mosquito, Aedes aegypti, ABSTRACT Dengue viruses (DENV) are the wildest transmitted arbovirus members of the family Flaviviridae, genus Flavivirus. Dengue viruses are composed of four serotypes, DENV1, 2, 3, and 4, and these viruses can cause dengue fever...
Title: Engineering a transgenic mosquito expressing blood meal induced siRNA to block dengue virus transmission in Aedes aegypti Background: Mosquito genome manipulation through germ-line transformation is a powerful tool for investigating gene structure and function of mosquitoes and is also a valuable method for the development of transgenic mos...
Mosquitoes are highly abounded and unlikely to be eradicated from the lands using conventional methods which have been used for several decades. The microinjection-based germline transformation techniques developed; efficiently transforms genes via transposable elements such as piggyBac, Hermes, Minos and Mariner tools enabling researchers to study...
Introduction:The genus Flavivirus of the family Flaviviridae includes several vector-borne viruses and dengue virus is one such virushavingfour serotypes (DENV-1,-2,-3 and 4). The clustered regularly interspaced short palindromic repeats/CRISPR- associated sequence 9 (CRISPR/Cas9) systemsis a self-dependent genome editing tool found in prokaryotesa...
The rearing of Aedes aegypti mosquitoes are complex and demanding due to several factors. The study on raring of Ae. aegypti mosquito vectors is important for mass rearing for the use of engineering dengue virus resistance in Ae. aegypti, genetic control, use of RNA interference and other experimental studies to control this primary vector which tr...
The genus Flavivirus of the family Flaviviridae includes several vector-borne viruses to which the four serotypes of dengue viruses (DENV-1,-2,-3 and 4) belong to and DENV viruses have a messenger like positive polarity, single-stranded RNA genome approximately 11kb in length which encodes three structural proteins (C-prM-E) and seven Non-Structura...
Leukemia is a cancer thatis belongs to blood and bone marrow cells, which are forms from hematopoietic progenitor cells and is being characterized by an abnormal proliferation and development of leukocytes and their precursors in the blood and bone marrow. Minimal residual disease is the name given to small numbers of leukemic cells that remain in...
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Genetic modification of Sri Lankan Aedes aegypti mosquitoes to get resistance to dengue virus using RNA interference mechanism.