Kalindu Ramyasoma

Kalindu Ramyasoma
University of Colombo · Department of Chemistry

Doctor of Philosophy

About

12
Publications
6,282
Reads
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6
Citations
Introduction
Kalindu Ramyasoma holds a doctorate in Molecular Entomology from the University of Colombo's Department of Chemistry, Faculty of Science. His main scientific interests include transgenic technology and its applications in the medical and agricultural sectors. His graduate thesis was titled "Engineering RNA interference-based resistance to dengue virus in Aedes aegypti vector mosquitoes."
Additional affiliations
October 2014 - September 2020
University of Colombo
Position
  • Research Assistant
Education
September 2009 - February 2014
University of Colombo
Field of study
  • Molecular Biology and Biochemistry

Publications

Publications (12)
Article
Full-text available
Background: Genetic modification offers opportunities to introduce artificially created molecular defence mechanisms to vector mosquitoes to counter diseases causing pathogens such as the dengue virus, malaria parasite, and Zika virus. RNA interference is such a molecular defence mechanism that could be used for this purpose to block the transmiss...
Article
Dengue viruses (DENV) are the wildest transmitted arbovirus members of the family Flaviviridae, genus Flavivirus. Dengue viruses are composed of four serotypes, DENV1, 2, 3, and 4, and these viruses can cause dengue fever and dengue haemorrhagic fever or dengue shock syndrome, when infecting humans. RNA interference (RNAi) is a self-defence mechani...
Article
The development of first ever transgenic animal in Sri Lanka and dengue transmission resistant mosquito, Aedes aegypti, ABSTRACT Dengue viruses (DENV) are the wildest transmitted arbovirus members of the family Flaviviridae, genus Flavivirus. Dengue viruses are composed of four serotypes, DENV1, 2, 3, and 4, and these viruses can cause dengue fever...
Conference Paper
Title: Engineering a transgenic mosquito expressing blood meal induced siRNA to block dengue virus transmission in Aedes aegypti Background: Mosquito genome manipulation through germ-line transformation is a powerful tool for investigating gene structure and function of mosquitoes and is also a valuable method for the development of transgenic mos...
Conference Paper
Mosquitoes are highly abounded and unlikely to be eradicated from the lands using conventional methods which have been used for several decades. The microinjection-based germline transformation techniques developed; efficiently transforms genes via transposable elements such as piggyBac, Hermes, Minos and Mariner tools enabling researchers to study...
Conference Paper
Introduction:The genus Flavivirus of the family Flaviviridae includes several vector-borne viruses and dengue virus is one such virushavingfour serotypes (DENV-1,-2,-3 and 4). The clustered regularly interspaced short palindromic repeats/CRISPR- associated sequence 9 (CRISPR/Cas9) systemsis a self-dependent genome editing tool found in prokaryotesa...
Conference Paper
The rearing of Aedes aegypti mosquitoes are complex and demanding due to several factors. The study on raring of Ae. aegypti mosquito vectors is important for mass rearing for the use of engineering dengue virus resistance in Ae. aegypti, genetic control, use of RNA interference and other experimental studies to control this primary vector which tr...
Conference Paper
The genus Flavivirus of the family Flaviviridae includes several vector-borne viruses to which the four serotypes of dengue viruses (DENV-1,-2,-3 and 4) belong to and DENV viruses have a messenger like positive polarity, single-stranded RNA genome approximately 11kb in length which encodes three structural proteins (C-prM-E) and seven Non-Structura...
Thesis
Full-text available
Leukemia is a cancer thatis belongs to blood and bone marrow cells, which are forms from hematopoietic progenitor cells and is being characterized by an abnormal proliferation and development of leukocytes and their precursors in the blood and bone marrow. Minimal residual disease is the name given to small numbers of leukemic cells that remain in...

Questions

Questions (6)
Question
How to identify male and female pupae of melon fly (Bactrocera cucurbitae)?
Question
Can I submit the results from 10ul qPCR reactions to a publication?What are the minimum requirements of qPCR to submit to a publication?
Question
Is food dye is essential for microinjection of insect eggs??
Question
RNA Extraction Skilled Personals

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Projects

Project (1)
Project
Genetic modification of Sri Lankan Aedes aegypti mosquitoes to get resistance to dengue virus using RNA interference mechanism.