Kailash N PandeyTulane University | TU · Department of Physiology
Kailash N Pandey
PhD
About
243
Publications
10,185
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
5,382
Citations
Publications
Publications (243)
The cardiac hormones atrial and brain natriuretic peptides (ANP and BNP) bind to guanylyl cyclase-A/natriuretic peptide receptor-A (GC-A/NPRA) that increases intracellular production of cGMP and stimulates natriuresis, diuresis, and vasorelaxant activities, thereby, reduces blood pressure (BP) and fluid volume. The role of ANP/NPRA signaling in pod...
We determined the epigenetic mechanisms regulating mean arterial pressure (MAP) and renal dysfunction in guanylyl cyclase/natriuretic peptide receptor‐A (GC‐A/NPRA) gene‐targeted mice. The Npr1 (encoding NPRA) gene‐targeted mice were treated with class 1 specific histone deacetylase inhibitor (HDACi) mocetinostat (MGCD) to determine the epigenetic...
Atrial and brain natriuretic peptides (ANP and BNP) bind to guanylyl cyclase-A/natriuretic peptide receptor-A (GC-A/NPRA), stimulating natriuresis and diuresis and reducing blood pressure (BP), but the role of ANP/NPRA signaling in podocytes (highly specialized epithelial cells covering the outer surfaces of renal glomerular capillaries) remains un...
Atrial natriuretic peptide (ANP), acting through guanylyl cyclase/natriuretic peptide receptor-A (GC-A/NPRA) regulates blood pressure and cardiac homeostasis. The disruption of Npr1 (encoding NPRA) in mice exhibits hypertension and provokes renal and cardiac disorder. However, the underlying mechanisms are not yet precisely determined. The objectiv...
The pioneering work of Dr Lewis K. Dahl established a relationship between kidney, salt, and high blood pressure (BP), which led to the major genetic-based experimental model of hypertension. BP, a heritable quantitative trait affected by numerous biological and environmental stimuli, is a major cause of morbidity and mortality worldwide and is con...
The objective of this study was to investigate the effect of class I-specific histone deacetylase inhibitor, mocetinostat (MGCD0103; MGCD) in the regulation of epigenetic mechanisms and renal pathology and disorders in Npr1 (coding for natriuretic peptide receptor A; NPRA) gene-disrupted haplotype mice. Male and female Npr1 gene-disrupted (1-copy;...
Picture was taken in 1985. Dr. Tadashi Inagami at Vanderbilt University.
On March 13, 2023, we lost a legend in our field. Tadashi Inagami, PhD who had retired to Pittsburgh, Pennsylvania, to be near family passed away at age 92. On behalf of his many friends, colleagues, and over 400 trainees including the authors of this tribute, we would like to...
Purpose of Study Atrial and brain natriuretic peptides (ANP, BNP) are circulating hormones of cardiac origin that play an important role in regulating blood pressure (BP) and cardiovascular homeostasis. The aim of this study was to investigate the role of natriuretic peptides (NPs) and microRNAs (miRs) in the ANP-dependent downregulation of NPRA, g...
Guanylyl cyclase/natriuretic peptide receptor-A (GC-A/NPRA) is activated by cardiac hormones atrial and brain natriuretic peptides (ANP, BNP) and produces intracellular second messenger cGMP. The physiological actions of ANP/NPRA/cGMP signaling cascade largely include diuresis, natriuresis, vasorelaxation, and organ protection, which maintains bloo...
The natriuretic peptides (NPs) hormone family, which consists mainly of atrial, brain, and C-type NPs (ANP, BNP, and CNP), play diverse roles in mammalian species, ranging from renal, cardiac, endocrine, neural, and vascular hemodynamics to metabolic regulations, immune responsiveness, and energy distributions. Over the last four decades, new data...
Cardiac hormones act on the regulation of blood pressure (BP) and cardiovascular homeostasis. These hormones include atrial and brain natriuretic peptides (ANP, BNP) and activate natriuretic peptide receptor-A (NPRA), which enhance natriuresis, diuresis, and vasorelaxation. In this study, we established the ANP-dependent homologous downregulation o...
The global targeted disruption of the natriuretic peptide receptor-A (NPRA) gene (Npr1) in mice provokes hypertension and cardiovascular dysfunction. The objective of this study was to determine the mechanisms regulating the development of cardiac fibrosis and dysfunction in Npr1 mutant mice. Npr1 knockout (Npr1−/−, 0-copy), heterozygous (Npr1+/−,...
Genetic ablation of guanylyl cyclase/natriuretic peptide receptor-A (GC-A/NPRA) in mice triggers cardiac remodeling associated with hypertrophy, fibrosis, and heart failure. The objective of this study was to determine the mechanisms regulating the developments of cardiac hypertrophy and fibrosis in global Npr1 (encoding NPRA) gene-knockout (KO) mi...
Atrial and brain natriuretic peptides (ANP and BNP) are produced in the heart, regulate blood pressure (BP) and fluid homeostasis through vasodilatory and diuretic actions. Both ANP and BNP act by binding to guanylyl cyclase/natriuretic peptide receptor-A (GC-A/NPR-A). Systemic disruption of Npr1(encoding GC-A/NPRA) leads to volume overload, high B...
Cardiac hormones atrial and brain natriuretic peptides (ANP and BNP) activate guanylyl cyclase/natriuretic peptide receptor-A (GC-A/NPRA) and produces the second messenger cGMP. cGMP activates downstream signaling and biological consequences of ANP/NPRA such as natriuresis, diuresis, vasorelaxation, antimitogenic responses, and cardiac and renal an...
The cardiac hormones, atrial and brain natriuretic peptides (ANP and BNP) bind to natriuretic peptide receptor-A (NPRA), which synthesizes the second messenger cGMP. The objective of this study was to determine the underlying mechanisms that regulate the development of cardiac hypertrophy, fibrosis, and congestive heart failure (CHF) in Npr1 (encod...
The discovery of atrial, brain, and C-type natriuretic peptides (ANP, BNP, and CNP) and their cognate receptors has greatly increased our knowledge of the control of hypertension and cardiovascular homeostasis. ANP and BNP are potent endogenous hypotensive hormones that elicit natriuretic, diuretic, vasorelaxant, antihypertrophic, antiproliferative...
Targeted-deletion of Npr1 gene (coding for guanylyl cyclase/natriuretic peptide receptor-A, GC-A/NPRA) exhibits hypertrophic and proliferative effects in target organs of Npr 1 gene-knockout mice. Fibrosis and hypertrophy are regulated by p21 Cip1 and p27 Kip1 , cell-cycle regulatory proteins that inhibit target cyclin and cyclin-dependent kinase (...
Cell‐cycle regulatory proteins (p21Cip1/p27Kip1) inhibit cyclin and cyclin‐dependent kinase (CDK) complex that promotes fibrosis and hypertrophy. The present study examined the role of CDK blockers, p21Cip1/p27Kip1 in the progression of renal fibrosis and dysfunction using Npr1 (encoding guanylyl cyclase/natriuretic peptide receptor‐A, GC‐A/NPRA) g...
Atrial and brain natriuretic peptides (ANP and BNP) bind to guanylyl‐cyclase/natriuretic peptide receptor‐A (GC‐A/NPRA) and regulate sodium excretion, water balance, vasorelaxation, and steroidogenesis; all directed to combat hypertension. ANP acting through NPRA lowers blood pressure and blood volume and protects the heart and vascular disorders a...
Atrial and brain natriuretic peptides (ANP, BNP) activate guanylyl cyclase/natriuretic peptide receptor‐A (GC‐A/NPRA), which plays a critical role in the regulation of blood pressure (BP) and fluid volume homeostasis, and inhibits the cell proliferation and fibrosis. Mice lacking functional Npr1 gene (coding for GC‐A/NPRA) exhibit pathophysiology o...
Atrial and brain natriuretic peptides (ANP and BNP) are cardiac hormones that elicit natriuretic, diuretic, vasorelaxant, and antiproliferative responses, all of which contribute to lowering blood pressure and blood volume. ANP and BNP bind to guanylyl cyclase ‐A/natriuretic peptide receptor ‐A (GC‐A/NPRA), which is considered to be the major natri...
The two vasoactive hormones, angiotensin II (ANG II; vasoconstrictive) and atrial natriuretic peptide (ANP; vasodilatory) antagonize the biological actions of each other. ANP acting through natriuretic peptide receptor-A (NPRA) lowers blood pressure and blood volume. We tested hypothesis that ANG II plays critical roles in the transcriptional repre...
The aim of the present study was to determine the effects of gene-knockout of guanylyl cyclase/natriuretic peptide receptor-A (GC-A/NPRA) on immunogenic responses affecting kidney function and blood pressure (BP) in Npr1 (coding for GC-A/NPRA) null mutant mice. We used female Npr1 gene-disrupted ( Npr1 -/- , 0-copy), heterozygous ( Npr1 +/- , 1-cop...
The objective of the present study was to gain insight into the effect of class I-specific histone deacetylase (HDAC) inhibitor, mocetinostat (MGCD0103; MGCD) on Npr1 (coding for natriuretic peptide receptor-A; NPRA) gene expression and regulation of renal pathology. Adult male and female Npr1 haplotype (1-copy; Npr1 +/- ), wild-type (2-copy; Npr1...
Mice bearing targeted gene mutations that affect the functions of natriuretic peptides (NPs) and natriuretic peptide receptors (NPRs) have contributed important information on the pathogenesis of hypertension, kidney disease, and cardiovascular dysfunction. Studies of mice having both complete gene disruption and tissue-specific gene ablation have...
Atrial and brain natriuretic peptides (ANP, BNP) activate guanylyl cyclase/natriuretic peptide receptor-A (GC-A/NPRA), which plays a critical role in the regulation of blood pressure and fluid volume homeostasis, and inhibits the cell proliferation and fibrosis. Mice lacking functional Npr1 (coding for GC-A/NPRA) gene exhibit hypertension and renal...
The present study was designed to determine the effects of gene-knockout of guanylyl cyclase/natriuretic peptide receptor-A (GC-A/NPRA) on immunogenic responses affecting kidney function and blood pressure (BP) in Npr1 (coding for GC-A/NPRA) null mutant mice. We used female Npr1 gene-disrupted ( Npr1-/-, 0-copy), heterozygous ( Npr1+/-, 1-copy), wi...
Endocytosis is a prominent clathrin-mediated mechanism for concentrated uptake and internalization of ligand-receptor complexes, also known as cargo. Internalization of cargo is the fundamental mechanism for receptor-dependent regulation of cell membrane function, intracellular signal transduction, and neurotransmission, as well as other biological...
The objective of the present study was to determine whether targeted-disruption of Npr1 gene (encoding for guanylyl cyclase/natriuretic peptide receptor-A; GC-A/NPRA) upregulates pro(renin) receptor (P)RR expression and leads to the activation of MAPKs in Npr1 gene-knockout mice. The Npr1 homozygous (Npr1 −/− ; 0-copy), heterozygous (Npr1 +/− ; 1-c...
Guanylyl cyclase/natriuretic peptide receptor‐A (GC‐A/NPRA) signaling plays a well‐defined role in the regulation of blood pressure and blood volume. Mice lacking functional Npr1 gene (coding for GC‐A/NPRA) exhibit hypertension, kidney disease, and heart failure; however, the mechanisms regulating Npr1 expression are not well understood. The object...
Atrial and brain natriuretic peptides (ANP and BNP) activate guanylyl cyclase/natriuretic peptide receptor‐a (GC‐A/NPRA), which regulates blood pressure through inhibition of renin‐angiotensin‐aldosterone system (RAAS). The aim of the present study was to determine whether targeted‐disruption of Npr1 (encoding GC‐A/NPRA) upregulates pro(renin) rece...
Genetic disruption of guanylyl cyclase/natriuretic peptide receptor‐A (GC‐A/NPRA) gene ( Npr1 ) in mice triggers cardiac hypertrophy, fibrosis, and remodeling associated with heart failure. However, the specific mechanisms of cardiac defects in Npr1 null mutant mice is not well understood. The objective of this study was to determine the mechanisms...
Atrial natriuretic peptide (ANP) is an endogenous cardiac hormone that binds to guanylyl‐cyclase/natriuretic peptide receptor‐A (GC‐A/NPRA) and regulates sodium excretion, water balance, and steroidogenesis; all directed toward the reduction of blood pressure and blood volume. The present study was aimed at understanding the mechanisms involved in...
Guanylyl cyclase/natriuretic peptide receptor‐A (GC‐A/NPRA) signaling activates natriuresis/diuresis, vasodilatation, and inhibits the cell proliferation and fibrosis. The objective of the present study was to gain insight into the mechanisms of Npr1 (coding for GC‐A/NPRA) expression and receptor function involving epigenetic regulatory mechanisms...
Cardiac hypertrophy is a major predictor of progressive heart disease and an adverse prognosis. Atrial and brain natriuretic peptides (ANP and BNP) bind to guanylyl cyclase/natriuretic peptide receptor‐A (GC‐A/NPRA) and exert the beneficial effects against hypertension and cardiac hypertrophy. The objective of the present study was to examine the e...
Targeted-disruption of guanylyl cyclase/natriuretic peptide receptor-A (GC-A/NPRA) gene ( Npr1 ) exhibits hypertension and provokes congestive heart failure in mice; however, the underlying mechanisms are not well clear. The objective of this study was to determine whether transforming growth factor-beta receptor (TGF-βR) antagonist, GW788388 inhib...
The alterations in the inflammatory system contribute to the pathogenesis of hypertension leading to end-organ damage. Guanylyl cyclase/natriuretic peptide receptor-A (GC-A/NPRA) gene ( Npr1 ) disruption elevates blood pressure and activates the immunogenic responses in mice. The goal of this study was to determine the effect of rapamycin on the ex...
The objective of the present study was to elucidate the systolic blood pressure (SBP) lowering effect of class I histone deacetylase (HDAC) inhibitor, mocetinostat (MGCD0103) by enhanced expression of natriuretic peptide receptor-A (NPRA) in gene ( Npr1 ). Adult male and female Npr1 heterozygous (1-copy; Npr1 +/- ), wild-type (2-copy; Npr1 +/+ ), a...
Natriuretic peptides (NPs) exert diverse effects on several biological and physiological systems, such as kidney function, neural and endocrine signaling, energy metabolism, and cardiovascular function, playing pivotal roles in the regulation of blood pressure (BP) and cardiac and vascular homeostasis. NPs are collectively known as anti-hypertensiv...
Cardiac hormones, atrial and brain natriuretic peptides (ANP and BNP), have pivotal roles in renal hemodynamics, neuroendocrine signaling, blood pressure regulation, and cardiovascular homeostasis. Binding of ANP and BNP to the guanylyl cyclase/natriuretic peptide receptor-A (GC-A/NPRA) induces rapid internalization and trafficking of the receptor...
Guanylyl cyclase/natriuretic peptide receptor‐A (GC‐A/NPRA) signaling activates natriuresis, diuresis, and vasodilatation, and inhibits the cell proliferation and fibrosis. Mice lacking functional Npr1 gene (coding for GC‐A/NPRA) exhibit hypertension and kidney disorders, however, the mechanisms regulating Npr1 expression and function are not well...
Atrial and brain natriuretic peptides (ANP and BNP) activate guanylyl cyclase/natriuretic peptide receptor‐a (GC‐A/NPRA), which regulates blood pressure through inhibition of renin‐angiotensin‐aldosterone system (RAAS). The present study was aimed to determine whether targeted‐disruption of Npr1 gene (coding for GC‐A/NPRA) upregulates pro(renin) re...
Disruption of natriuretic peptide receptor-A (NPRA) gene ( Npr1 ) activates the pro-inflammatory responses, which contributes to the pathogenesis of hypertension and end-organ damage. The objective of this study was to determine the kinetic responses of pro- and anti-inflammatory cytokines in Npr1 gene-knockout (KO) mice. Npr1 0-copy ( Npr1 -/- ),...
The objective of the present study was to determine the effect of class I histone deacetylase (HDAC) inhibitor, mocetinostat (MGCD0103) on the expression of Npr1 (coding for natriuretic peptide receptor-A; NPRA) and renal pathology in Npr1 gene-disrupted haplotype (50% of normal) mice. Adult male Npr1 haplotype (1-copy; Npr1 +/- ), wild-type (2-cop...
Guanylyl cyclase/natriuretic peptide receptor-A (GC-A/NPRA) plays a critical role in the regulation of blood pressure and fluid volume homeostasis. Mice lacking functional Npr1 (coding for GC-A/NPRA) exhibit hypertension and congestive heart failure. However, the underlying mechanisms remain largely less clear. The objective of the present study wa...
Introduction: Mice lacking functional guanylyl cyclase/natriuretic peptide receptor-A (GC-A/NPRA) gene ( Npr1 ) exhibit hypertension, kidney disease, and heart failure. In this study, we investigated the effect of butyric acid, a histone deacetylase (HDAC) inhibitor and all-trans retinoic acid (ATRA) hybrid drug (ATRA-BA) on the attenuation of rena...
Introduction: Targeted-ablation of guanylyl cyclase/natriuretic peptide receptor-A (GC-A/NPRA) gene ( Npr1 ) in mice exhibits hypertension and provokes congestive heart failure; however, the underlying mechanisms are not well understood.
Hypothesis: To determine whether transforming growth factor-beta 1 (TGF)-β1 receptor antagonist, GW788388 inhibi...
The objective of the present study was to examine the repressive effect of transforming growth factor beta 1 (TGF-β1) in the regulation of Npr1 (coding for guanylyl cyclase/natriuretic peptide receptor-A; GC-A/NPRA) gene expression and vascular signaling. The rat thoracic aortic vascular smooth muscle cells (RTASMC) and denuded aortic rings were cu...
The objective of the present study was to determine the effect of a hybrid drug of sodium butyrate (NaBu), a histone deacetylase (HDAC) inhibitor and all-trans retinoic acid (ATRA) on the attenuation of renal inflammation and fibrosis in Npr1 gene-disrupted mutant mice. Adult (18-20 week old) male Npr1 gene-disrupted heterozygous (1-copy; Npr1 +/-...
In the present study, we examined the effect of genetically determined differences in the guanylyl cyclase-A/natriuretic peptide receptor-A (GC-A/NPRA) gene (Npr1) copies on the expression levels of proinflammatory mediators, matrix metalloproteinases (MMPs), and cardiac hypertrophic markers in Npr1 gene-targeted mouse models. We also determined wh...
The objective of this study was to delineate the critical role of endocytic signal in intracellular sorting and signaling of receptor into these compartments. We have identified a FQQI (Phe790, Gln791, Gln792 and Ile793) motif in the cytoplasmic tail of NPRA sequence and mutated with alanine residues (FQQI/AAAA) using QuickChange II site-directed m...
Cardiac hormone, atrial natriuretic peptide (ANP) activates guanylyl cyclase/natriuretic peptide receptor-A (GC-A/NPRA), which produces second messenger cGMP and lowers blood pressure and blood volume. The objective of this study was to visualize the internalization and intracellular trafficking of NPRA in the subcellular compartments of intact cel...
The objective of this study was to delineate the critical role of endocytic signal in intracellular sorting and signaling of receptor into these compartments. We have identified a FQQI (Phe ⁷⁹⁰ , Gln ⁷⁹¹ , Gln ⁷⁹² and Ile ⁷⁹³ ) motif in the cytoplasmic tail of NPRA sequence and mutated with alanine residues (FQQI/AAAA) using QuickChange II site‐dir...
Guanylyl cyclase/natriuretic peptide receptor‐A (GC‐A/NPRA)/cGMP signaling plays a well‐defined role in the regulation of blood pressure and blood volume. Mice lacking functional Npr1 gene (coding for GC‐A/NPRA) exhibit hypertension, kidney disease, and heart failure. The objective of the present study was to determine the combined effect of sodium...
Cardiac hormone, atrial natriuretic peptide (ANP) activates guanylyl cyclase/natriuretic peptide receptor‐A (GC‐A/NPRA), which produces second messenger cGMP and lowers blood pressure and blood volume. The objective of this study was to visualize the internalization and intracellular trafficking of NPRA in the subcellular compartments of intact cel...
The objective of this study was to determine the role of transforming growth factor-beta 1 (TGF-β1) in transcriptional regulation and function of guanylyl cyclase-A/natriuretic peptide receptor-A (GC-A/NPRA) gene (Npr1) and whether a cross-talk exists between these two hormonal systems in target cells. After treatments of primary cultured rat thora...
Binding of cardiac hormone, atrial natriuretic peptide (ANP) to transmembrane guanylyl cyclase/natriuretic peptide receptor-A (GC-A/NPRA), produces the intracellular second messenger cGMP in target cells. To delineate the critical role of endocytic signal in intracellular sorting of the receptor, we have identified a FQQI (Phe790, Gln791, Gln792, a...
Atrial natriuretic peptide (ANP) activates guanylyl cyclase/natriuretic peptide receptor-A (GC-A/NPRA), which lowers blood pressure and blood volume. The objective of this study was to visualize internalization and trafficking of enhanced green fluorescence protein (eGFP)-tagged NPRA (eGFP-NPRA) in human embryonic kidney-293 (HEK-293) cells, using...
The objective of the present study was to determine the combined effect of sodium butyrate (NaBu), a histone deacetylase (HDAC) inhibitor and all-trans retinoic acid (ATRA) on attenuation of renal fibrosis and inflammation in Npr1 (coding for natriuretic peptide receptor-A) gene-disrupted mutant mice. Adult (18-20 week old) male Npr1 gene-disrupted...
The binding of atrial and brain natriuretic peptides (ANP and BNP) to guanylyl cyclase-A/natriuretic peptide receptor-A (GC-A/NPR-A) produces second messenger cGMP, which lowers blood pressure and prevents cardiovascular events. The objective of the present study was to examine the repressive effect of transforming growth factor (TGF-β1) in the reg...
Genetic disruption of guanylyl cyclase/natriuretic peptide receptor-A (GC-A/NPRA) gene (Npr1) in mice exhibits high blood pressure, cardiac hypertrophy, fibrosis, and remodeling leading to congestive heart failure. The objective of this study was to determine the mechanisms regulating the development of fibrosis in Npr1 gene-disrupted mice hearts....
The targeted endocytosis and redistribution of transmembrane receptors among membrane-bound subcellular organelles are vital for their correct signaling and physiological functions. Membrane receptors committed for internalization and trafficking pathways are sorted into coated vesicles. Cardiac hormones, atrial and brain natriuretic peptides (ANP...
The objective of the present study was to examine the genetically determined differences in the natriuretic peptide receptor-A (NPRA) gene (Npr1) copies affecting the expression of cardiac hypertrophic markers, proinflammatory mediators, and matrix metalloproteinases (MMPs) in a gene-dose-dependent manner. We determined whether stimulation of Npr1...
Binding of cardiac hormone, atrial natriuretic peptide (ANP) to the guanylyl cyclase/natriuretic peptide receptor‐A (GC‐A/NPRA), induces the rapid internalization and trafficking via the endo‐lysosomal compartments with concurrent generation of cGMP. To delineate the critical role of endocytic signal in intracellular sorting of receptor into these...
Toll‐like Receptor‐4 (TLR4) plays a potential role in the innate immune responses by sensing endogenous danger signals associated with the stimulation of pro‐inflammatory molecules. The objective of the present study was to elucidate the impact of guanylyl cyclase/natriuretic peptide receptor‐A (GC‐A/NPRA) gene ( Npr1 ) disruption on TLR4 expressio...
Binding of cardiac hormone, atrial natriuretic peptide (ANP) to the guanylyl cyclase/natriuretic peptide receptor-A (GC-A/NPRA), induces the rapid internalization and trafficking via the endo-lysosomal compartments with concurrent generation of cGMP. To delineate the critical role of endocytic signal in intracellular sorting of receptor into these...
Toll-like Receptor-4 (TLR4) plays a potential role in the innate immune responses by sensing endogenous danger signals associated with the stimulation of pro-inflammatory molecules. The objective of the present study was to elucidate the impact of guanylyl cyclase/natriuretic peptide receptor-A (GC-A/NPRA) gene (Npr1) disruption on TLR4 expression...
Purpose of Study: Binding of cardiac hormone, atrial natriuretic peptide
(ANP) to the guanylyl cyclase/natriuretic peptide receptor-A (GC-A/NPRA),
induces the rapid internalization and subcellular trafficking via the endolysosomal
compartments with concurrent generation of cGMP. The objective
of this study was to visualize the internalization and i...
Thus far, three related natriuretic peptides (NPs) and three distinct sub-types of cognate NP receptors have been identified and characterized based on the specific ligand binding affinities, guanylyl cyclase activity, and generation of intracellular cGMP. Atrial and brain natriuretic peptides (ANP and BNP) specifically bind and activate guanylyl c...
The objective of the present study was to delineate the mechanisms of guanylyl cyclase/natriuretic peptide receptor-A (GC-A/NPRA) gene (Npr1) expression in vivo. We utilized all-trans retinoic acid (ATRA) and histone deacetylase (HDAC) inhibitor, sodium butyrate (NaBu) to examine the expression and function of Npr1 using gene-disrupted heterozygous...
Cardiac hormone, atrial natriuretic peptide (ANP) modulates blood pressure and blood volume by activation of guanylyl cyclase/natriuretic peptide receptor-A (GC-A/NPRA), which produces the second messenger cGMP. The aim of the present study was to examine the role of small sequence motifs in the internalization and intracellular trafficking pathway...
Cardiac hormone, atrial natriuretic peptide (ANP) modulates blood pressure and blood volume by activation of guanylyl cyclase/natriuretic peptide receptor‐A (GC‐A/NPRA), which produces the second messenger cGMP. The aim of the present study was to examine the role of small sequence motifs in the internalization and intracellular trafficking pathway...
Mice carrying targeted‐disruption of the natriuretic peptide receptor‐A (GC‐A/NPRA) gene ( Npr1 ) have demonstrated cardiac hypertrophy with increased collagen deposition and fibrosis. The objective of this study was to determine the mechanisms regulating the development of fibrosis in Npr1 gene‐disrupted mice hearts. The Npr1 null mutant (Npr1 ‐/‐...
Mice lacking functional guanylyl cyclase/natriuretic peptide receptor‐A (GC‐A/NPRA) gene (Npr1) exhibit hypertension, kidney disease, and heart failure. The objective of the present study was to elucidate the effect of all‐trans retinoic acid (ATRA) and histone deacetylase inhibitor, sodium butyrate (NaBu) on attenuation of renal fibrosis and remod...
The objective of this study was to examine whether genetically determined differences in guanylyl cyclase/natriuretic peptide receptor-A (GC-A/NPRA) gene (Npr1) affect cardiac expression of pro-inflammatory cytokines, hypertrophic markers, nuclear factor kappa-B (NF-kB), and activating protein-1 (AP-1) in a Npr1 gene-dose-dependent manner. In the p...
Atrial natriuretic peptide (ANP) binds guanylyl cyclase-A/natriuretic peptide receptor-A (GC-A/NPRA) and produces the intracellular
second messenger, cGMP, which regulates cardiovascular homeostasis. We sought to determine the function of histone deacetylases
(HDACs) in regulating Npr1 (coding for GC-A/NPRA) gene transcription, using primary mouse...
Guanylyl cyclase/natriuretic peptide receptor-A (GC-A/NPRA) is the principal loci involved in the regulatory actions of cardiac hormones namely, atrial and brain natriuretic peptide (ANP and BNP). Interaction of ANP and BNP with GC-A/NPRA produces the intracellular second messenger cGMP, which plays a central role in the pathophysiology of hyperten...
The cardiac hormone atrial natriuretic peptide (ANP) regulates blood pressure and blood volume by
activation of guanylyl cyclase/natriuretic peptide receptor-A (GC-A/NPRA) and subsequent generation of
intracellular second messenger cGMP. The objective of this study was to visualize the internalization and intracellular trafficking of NPRA in the s...
Atrial natriuretic peptide (ANP) is a cardiac hormone that modulates blood pressure and blood volume by activation of guanylyl cyclase/natriuretic peptide receptor-A (GC-A/NPRA) and subsequent generation of intracellular second messenger cGMP. The aim of the present study was to examine the role of small sequence motifs in the internalization and t...
Regulation of gene expression plays an obligatorily role in the modification of chromatin structure that dynamically attenuates cardiac hypertrophy. In order to culminate the role of epigenetic regulators in the heart tissue, the current study was undertaken to elucidate the effect of histone deacetylase (HDAC) inhibitor, sodium butyrate (SB) in ca...
Mice lacking functional Npr1 gene (coding for guanylyl cyclase/natriuretic peptide receptor-A, GC-A/NPRA) exhibit hypertension and kidney disease, however, the mechanisms regulating Npr1 expression are not well understood. The objective of the present study was to determine the effect of all- trans retinoic acid (ATRA) on Npr1 gene expression and r...
Atrial natriuretic peptide (ANP) is a cardiac hormone that modulates blood pressure and blood volume by activation of guanylyl cyclase/natriuretic peptide receptor-A (GC-A/NPRA) and subsequent generation of intracellular second messenger cGMP. The aim of the present study was to examine the role of small sequence motifs in the internalization and t...
Guanylyl cyclase/natriuretic peptide receptor-A (GC-A/NPRA) is the principal receptor for the regulatory action of atrial and brain natriuretic peptides (ANP and BNP) and an important effector molecule in controlling of extracellular fluid volume and blood pressure homeostasis. We have utilized RNA interference to silence the expression of GC-A/NPR...
Cardiac hormone atrial natriuretic peptide (ANP) modulates blood pressure and blood volume by activation of the guanylyl cyclase/natriuretic peptide receptor-A (GC-A/NPRA) and subsequent generation of intracellular second messenger cGMP. Advances in the development of proteins conjugated with the enhanced green fluorescence protein (eGFP) provide f...