Kai Battenberg

Kai Battenberg
RIKEN, Yokohama · CSRS

PhD

About

36
Publications
3,095
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383
Citations

Publications

Publications (36)
Preprint
Frankia cluster-2 are diazotrophs that engage in root nodule symbiosis with host plants of the Cucurbitales and the Rosales. They are rarely found in the soil in the absence of their hosts. Previous studies have shown that an assimilated nitrogen source, presumable arginine, is exported to the host in nodules of Datisca glomerata (Cucurbitales), bu...
Article
Full-text available
Frankia strains induce the formation of nitrogen-fixing nodules on roots of actinorhizal plants. Phylogenetically, Frankia strains can be grouped in four clusters. The earliest divergent cluster, cluster-2, has a particularly wide host range. The analysis of cluster-2 strains has been hampered by the fact that with two exceptions, they could never...
Article
Full-text available
Two types of nitrogen-fixing root nodule symbioses are known, rhizobial and actinorhizal symbioses. The latter involve plants of three orders, Fagales, Rosales, and Cucurbitales. To understand the diversity of plant symbiotic adaptation, we compared the nodule transcriptomes of Datisca glomerata (Datiscaceae, Cucurbitales) and Ceanothus thyrsifloru...
Data
Head-to-tail comparisons of MS/MS spectra of the most abundant flavonoids identified in D. glomerata. Reference library spectra are shown in red, experimental spectra are given in blue. Metadata include the similarity dot product and reverse dot product scores, InChIKey, and the structure of annotated compounds.
Data
Head-to-tail comparisons of MS/MS spectra of the most abundant flavonoids identified in M. sativa. Reference library spectra are shown in red, experimental spectra are given in blue. Metadata include the similarity dot product and reverse dot product scores, InChIKey, and the structure of annotated compounds.
Data
Collection data for root and nodule samples of D. glomerata and M. sativa.
Data
Number of flavonoids annotated in each class in D. glomerata and M. sativa roots and nodules and chi-square test comparing the distribution of classes by proportion of total flavonoids in each host.
Data
Flavonoid metabolome data obtained from LCMS analyses of D. glomerata and M. sativa nodules and roots.
Article
Full-text available
Plants within the Nitrogen-fixing Clade (NFC) of Angiosperms form root nodule symbioses with nitrogen-fixing bacteria. Actinorhizal plants (in Cucurbitales, Fagales, Rosales) form symbioses with the actinobacteria Frankia while legumes (Fabales) form symbioses with proteobacterial rhizobia. Flavonoids, secondary metabolites of the phenylpropanoid p...
Article
The root nodule symbiosis of plants with nitrogen-fixing bacteria impacts global nitrogen cycles and food production but is restricted to a subset of genera within a single clade of flowering plants. To explore the genetic basis for this scattered occurrence, we sequenced the genomes of ten plant species covering the diversity of nodule morphotypes...
Article
The genus Frankia comprises a group of nitrogen-fixing actinobacteria that form root-nodule symbioses with perennial dicotyledonous plants in the nitrogen-fixing clade. These bacteria have been characterized phylogenetically and grouped into four clusters (clusters 1-4). Cluster 2 contains mostly uncultured strains that induce nodules on species of...
Article
Full-text available
Background Identifying orthologous genes is an initial step required for phylogenetics, and it is also a common strategy employed in functional genetics to find candidates for functionally equivalent genes across multiple species. At the same time, in silico orthology prediction tools often require large computational resources only available on co...
Article
Full-text available
Importance: Biological nitrogen fixation is a bacterial process that accounts for a major fraction of net new nitrogen input in terrestrial ecosystems. Transfer of fixed nitrogen to plant biomass is especially efficient via root nodule symbioses, which represent evolutionarily and ecologically specialized mutualistic associations. Frankia spp. (Ac...
Article
Full-text available
Background The ability to establish root nodule symbioses is restricted to four different plant orders. Soil actinobacteria of the genus Frankia can establish a symbiotic relationship with a diverse group of plants within eight different families from three different orders, the Cucurbitales, Fagales and Rosales. Phylogenetically, Frankia strains c...
Article
Full-text available
Frankia strains are nitrogen-fixing soil actinobacteria that can form root symbioses with actinorhizal plants. Phylogenetically, symbiotic frankiae can be divided into three clusters, and this division also corresponds to host specificity groups. The strains of cluster II which form symbioses with actinorhizal Rosales and Cucurbitales, thus display...

Questions

Question (1)
Question
I want to do Fluorescence Activated Cell Sorting on plant roots (based on GFP fluorescence) to collect material for RNA-seq.
I would like to select out specific cell type (defined by the expression of gene A) at a specific developmental stage (defined by the expression of gene B).
So I would like to find a way to activate GFP upon expression of both A and B (sort of the opposite of a bicistronic vector).
What is a good way to do this?

Projects

Project (1)