Kaan Hürkan

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• Doctor of Philosophy
• Professor (Associate) at Iğdır Üniversitesi

Fast, accurate and affordable identification of food products is important to ensure authenticity and safety. There are various apricot (Prunus armeniaca L.) cultivars are being produced in Turkey. Each cultivar differs in quality and purpose of use. In this paper, we aimed to develop an easy and reliable method, Barcode High-Resolution Melting (Ba...

The COVID-19 pandemic continues infecting people causing deaths globally. Although various medicines have been tried to combat with COVID-19, there is no medicine or treatment that has been validated yet. People have been using natural products for centuries against bacterial and viral illnesses. This study aimed to test the effects of the biomolec...

The pistachio nut (Pistacia vera L.) is a popular nut which is beneficial for health. As a result of its high price, adulteration of pistachio products with other foods is common. The green pea (Pisum sativum L.) is similar to pistachio in colour and texture when both are in a fine powder form, therefore powdered pistachio products are often adulte...

Unlabelled: Accurate identification of animal species sources in milk have become quite important due to adulteration of high-priced milk types in the dairy industry. To date, milk identification methods have mainly depended on biochemical properties or physical properties detected by spectroscopic methods. The current study aimed to develop an ea...

Plants have been struggled by changing environmental conditions for millions of years. Fortunately, plants have the ability to accommodate it by fine-tuning itself. Genes, which are commonly known as functional unit of heredity in books, are secret weapons of plants that are always ready to be used for any changing circumstances. These DNA-level un...

Wheat is a vital global staple, with Triticum durum Desf. (durum wheat) primarily used for pasta and couscous, and Triticum aestivum L. (bread wheat) widely cultivated for baking. Differentiating these species, especially in processed forms, poses significant challenges due to their genetic similarity and overlapping end-use applications. This stud...

In tissue cultures, surface sterilization is one of the most crucial concerns. The subsequent steps cannot be completed without success in the surface sterilization phase. Because of this, the most important factor affecting a culture's efficacy is the surface sterilization of plant parts (explants). When surface sterilizing explants, sterilization...

In recent times, there has been a surge in the use of nanomaterials within biomedical. These materials have gained significant interest for their potential in several applications, including the development of artificial organs and tissues, drug delivery systems, and gene therapy. This increasing interest may be attributed to the unique properties...

Graphene, a nanomaterial, is recognized as a two-dimensional (2D) carbon form, one of the universe's most prevalent elements. Despite its short history since its discovery, it exhibits extraordinary thermal, electrical, optical, and mechanical properties thanks to its high surface area, high electron mobility, biocompatible structure, and high adso...

Plants possess a natural genetic diversity that is used for various scientific research purposes. This diversity is useful in deoxyribonucleic acid (DNA) fingerprint and barcode studies, which have many applications in scientific research such as determining the relationships between plant species, determining parentage, creating genome maps, and d...

Türkiye, with an apricot (Prunus armeniaca L.) production amount of 833,398 tons per year, ranks first in fresh apricot production and dried apricot export in the world. Malatya, Iğdır, and Elazığ constitute the main apricot production centers in Turkey. Many table and dried apricot cultivars have been grown in Türkiye. Local apricot cultivars such...

Bacterial canker of stone fruits caused by Pseudomonas syringae pv. syringae (Pss) and Pseudomonas syringae pv. morsprunorum (Psm race‐1 /Psm race‐2 ) may lead to significant yield and crop losses in apricot ( Prunus armeniaca L.) cultivation areas in Türkiye. Strains pathogenic to apricot were isolated from trees with symptoms (mainly necrotized b...

https://shop.elsevier.com/books/genomics-transcriptomics-proteomics-and-metabolomics-of-crop-plants/husen/978-0-323-95989-6

Background Geotrichum is a genus of fungi found in different habitats throughout the world. Although Geotrichum and its related species have been extensively reclassified and taxonomically revised, it is still the target for many researches. Methods and results In this study, phenotypic and molecular genetics comparisons were performed between Geo...

Diseases, damage and physiological changes may occur that will affect the growth, development and survival of plants outside of the optimum growth conditions. The factors that cause these unfavourable conditions are called stress. Stress factors are divided into biotic (plants, microorganisms, animals and anthropogenic effects) and abiotic (radiati...

ÖZET Ekilebilir verimli tarım alanlarının artmaya devam eden insan popülasyonu, küresel iklim değişikliği, toprak erozyonu, toprakta tuzlanma ve şehirleşme gibi nedenlerle azalması gibi başlıca etmenler bireylerin gıdaya erişimini gün geçtikçe güçleştirmektedir. Çiftçiler, gittikçe daha fazla gıdaya ihtiyaç duyan dünyayı beslemek için daha dar alan...

Türkiye meets about 7% of the world tomato production. The most severe effects of climate change are seen in agriculture. The increase of salinity in agricultural lands reduces the usable area and affects the growth, development and yield of the products grown. The salinity problem in the Iğdır Plain, which has a microclimate feature, causes the re...

Ülkemiz, dünya domates üretiminin yaklaşık %7'sini karşılamaktadır. Tüm dünyada olduğu gibi ülkemizde de iklim değişikliğinin en şiddetli etkileri tarımda görülmektedir. Tarım arazilerindeki tuzluluk artışı kullanılabilir alanı azaltmakta, yetiştirilen ürünlerde ise büyüme, gelişme ve verimi etkilemektedir. Mikroklima özelliği gösteren Iğdır Ovası'...

Tarımda ueni teknolojiler

ÖNSÖZ Tarım; bitkisel ve hayvansal ürünlerin üretilmesi, kullanımı, hazırlanması, pazarlanması ve dağıtımını ele alan bitki ve hayvan yetiştirme sanatı ve bilimidir. Tarım, dünyanın gıda, içecek, ilaç, kozmetik, yakacak, barınak, kumaş ve boya gibi ihtiyaçlarını karşılar. Ekosistemin üreticileri olan bitkiler, güneş ışığından aldığı enerjiyi diğer...

To protect consumers against food adulteration, it is necessary to strictly test and evaluate the food products to detect adulteration. The pistachio nut (Pistacia vera L.) is a valuable nut which is beneficial for health. Although it is an overproduced nut, pistachio is still an economically valuable nut due to its high consumption. As a result of...

Background The pistachio nut ( Pistacia vera L.) is a popular nut which is beneficial for health. As a result of its high price, adulteration of pistachio products with other foods is common. The green pea ( Pisum sativum L.) is similar to pistachio in colour and texture when both are in a fine powder form, therefore powdered pistachio products are...

The pistachio nut ( Pistacia vera L.) is a popular nut which is beneficial for health. As a result of its high price, adulteration of pistachio products with other foods is common. The green pea ( Pisum sativum L.) is similar to pistachio in colour and texture when both are in a fine powder form, therefore powdered pistachio products are often adul...

The apricot cultivar Prunus armeniaca cv. Şalak (registered as "Iğdır Kayısısı") is a Protected Designation of Origin (PDO) apricot and produced in Aras Basin (Iğdır, Turkey) region. Authenticating the special products, which has adulteration potential, by DNA based methods is reliable and cost-effective. The aim of the current study is to distingu...

In this study chorismate synthase, chorismate mutase and anthranilate synthase in the soybean genome (GmCS, GmCM, and GmAS or collectively named as GmAAAs in this study), were investigated using bioinformatics and expression analyses under salt and drought stresses. It is found that there are multiple copies of GmCM, GmCS, and GmAS in the soybean g...

High Resolution Melting Principal Component Analysis on R

Internal Transcribed Spacer (ITS) is one of the most used barcoding region for the molecular phylogenetics and barcoding of orchids. Our aim in this study is to test the reliability of ITS on barcoding of closely related Neotinea spp., including Neotinea tridentata, Neotinea ustulata subsp. ustulata and Neotinea ustulata subsp. aestivalis, by compa...

Identifying the originality and detecting the authentication of the processed and unprocessed commercial food products ensure food safety. Food adulteration of food products with high commercial value by cheap additives could threaten human health. In this study, we generated and tested five DNA barcodes (ITS, LEAFY, matK, rbcL, ycf1) of the Turkis...

Yeşil devrim ile başlayan tarımsal ürünlerdeki üretim artışı politikası, tarım yapılabilecek alan sayısının her geçen gün azalması, sanayi kaynaklı iklim değişikliğinin tarım bitkilerinde verim düşüşüne sebep olması ve en önemlisi insan nüfusunun hızlanarak artması ile sekteye uğramış durumdadır. Dünya nüfusunun 2050 yılında yaklaşık 10 milyara ula...

In this study we evaluated the secondary metabolites, total phenolic (TPC) and flavonoid contents (TFC), antioxidant and antimicrobial activities of salep orchids, Anacamptis morio, Anacamptis pyramidalis, Neotinea tridentata, Ophrys mammosa, Ophrys lutea, and Ophrys speculum. DPPH free radical scavenging assay was used to determine the antioxidant...

Orkideler (Orchidaceae), yeryüzünün neredeyse tamamında yayılış gösteren ve çiçekli bitkiler içerisinde en çok tür içeren ikinci familyadır. Bu kalabalık bitki grubunun sınıflandırılması ve filogenetiğinin ortaya çıkarılmasında sadece morfolojiye dayalı yöntemler yetersiz kalmaktadır. Bu çalışmada Orchis anatolica Boiss. türünün çekirdek DNA’sının...

Bu çalışmada; Anacamptis morio, Anacamptis pyramidalis, Neotinea tridentata, Ophrys mammosa, Ophrys lutea ve Ophrys speculum salep orkidelerinin antioksidan ve antimikrobiyal aktiviteleri ölçülmüştür. DPPH serbest radikal temizleme yöntemi ile bitkilerin n-hekzan, kloroform, methanol ve su özütlerinde antioksidan aktiviteler belirlenmiştir. Antimik...

Due to the increasing demand and over collection of orchids from nature to produce salep, scientists have been led to search for more efficient ways to propagate these specific orchids in vitro. This present study compares germination performances of two commercial (Orchimax and Knudson C) and one specially prepared orchid growth media (SV), on eco...

Reference genes are essential for the normalisation of the expression data of quantitative real-time PCR for the purposes of validation. Although several reference genes have been validated for the olive, comprehensive analyses including an excessive number of candidate reference genes still require study in various olive tissue samples. In this wo...

zet: DNA barkodlama son 30 yıldır bitki türlerinin tayininde ve filogenetik çalışmalarında kullanılan ve popülerliği her geçen gün artan etkili bir tekniktir. Bu teknikte çekirdekten veya plastidlerden elde edilen kısa DNA dizileri analiz edilerek canlılar tür seviyesinde ayırt edilmeye çalışılmaktadır. Çekirdek kökenli barkod bölgeleri, plastid kö...

Abstract: DNA barcoding is a technique that has been widely used to identify species and discover phylogenetic utilities of species for last 30 years. The main goal of this technique is to discriminate organisms at species level by short DNA fragments originated from nucleus or plastids. Although nucleus originated barcoding regions provide more da...

Zeytin (Olea europaea L.) Dokularında Real-Time Kantitatif Polimeraz Zincir Reaksiyonu Temelli Gen Anlatım Çalışmalarında Kullanılmak Üzere Referans Genlerin Belirlenmesi

The increasing collection of orchids from nature to produce salep has led to scientists searching for more efficient ways to propagate orchids in vitro. The present study compares germinative performances of two commercial (Orchimax and Knudson C) and one special prepared orchid growth media (SV) on economically and medicinally important salep orch...

In this study, we determined the structure of DNA methyltransferase (DNMT) genes in apomict and sexual Boechera species and investigated the expression levels during seed development. Protein and DNA sequences of diploid sexual Boechera stricta DNMT genes obtained from Phytozome 10.3 were used to identify the homologues in apomicts, Boechera holboe...

Expression analysis of the genes involved in strigolactone biosynthesis in olive

Molecular characterization of olive Strigolactone biosynthesis genes

In Turkey the tubers of about 120 orchid species are widely collected for manufacturing the traditional drink salep. In this study, we focused on the in vitro germination of the pollen of the salep orchid species Ophrys mammosa, Orchis provincialis, Anacamptis morio subsp. morio, Orchis simia and Neotinea tridentata and discussed the potential effe...

Graveyards in Turkey are widely known among orchidologists as places where several orchid (Orchidaceae) taxa can be found, including some very rare and localized ones. Graveyards are less strongly affected by landscape-altering human activities than other habitats because of their special cultural roles and religious privileges. In this study we pe...

In this study, in vitro germination capabilities of pollen cluster and pollinium of the taxa Ophrys mammosa (Desf.), Orchis provincialis (Balb.), Anacamptis morio (L.) R.M. Bateman, Pridgeon & M.W. Chase subsp. morio, Orchis simia (Lam.) and Neotinea tridentata (Scop.) R.M. Bateman, Pridgeon & M.W. Chase were studied on three different media; Knuds...

In our study, we aim to (i) confirm phylogenetic structure, (ii) discuss to have identical ribotypes and (iii) reveal hybridisation possibility of closely related Neotinea tridentata (Scop.) R.M.Bateman, Pridgeon & M.W.Chase and Neotinea ustulata (L.) R.M.Bateman, Pridgeon & M.W.Chase spread in Mediterranean and extra–Mediterranean region of Europe...

Our goal was to (i) confirm the phylogenetic structure, (ii) discuss to have identical ribotypes and (iii) reveal hybridisation possibility of closely related Neotinea tridentata (Scop.) and Neotinea ustulata (L.) spread in the Mediterranean and extra–Mediterranean regions of Europe / Anatolia based on the nrITS and accD–psaI DNA sequences. Previou...

Neotinea tridentata (Scopoli) R.M. Bateman, Pridgeon & M.W. Chase, özellikle orta ve Doğu Akdeniz Bölgesi’ nde, Orta Avrupa’ da ise Hessen ve Thüringen’ de adacıklar halinde yayılış gösteren, Türkiye’ de ise; Karadeniz, Marmara, Akdeniz ve Doğu Anadolu Bölgeleri başta olmak üzere, Orta Anadolu ve Güney Doğu Anadolu Bölgeleri haricinde Türkiye’ nin...

×Comptoglossum agiasense was firstly described relatively recently in 2004 as a rare intergeneric hybrid between Comperia comperiana and Himantoglossum montis-tauri on the Island of Lesvos (Greece). In the following years (2009 and 2010) the hybrid was also reported from different places in district Samsun (Turkey). However, the parentage is highly...

Ozon incelmesi, dünya yüzeyine ulaşan ultraviyole (UV) radyasyonunda önemli artışlara neden olmaktadır (Du ve ark., 2010). Yeryüzüne ulaşan UV radyasyonunun spektrumu, düşük enerji (UV-A, 320-400 nm), yüksek enerji (UV-B, 280-320 nm) ve UV-C (254-280 nm) bölgelerine ayrılmaktadır. Radyasyonun herhangi bir dozuna bitkilerin verdiği yanıtlar farklılı...

In this study, molecular phylogenetic and taxonomic affinities of Turkish representatives of Orchis anatolica Boiss. and Neotinea tridentata (Scop.) R.M. Bateman, Pridgeon & M.W. Chase (Orchidaceae) species were compared with some Turkish terrestrial orchid species using the technique of sequencing the whole ribosomal ITS region (ITS1-5.8S -ITS2) o...

Lisans programlarında okutulan sitoloji dersleri için laboratuvar uygulamaları kılavuzu. Laboratory practice book for cytology course in bechalor degree.

In the present study, the number of leaves of stems, total bulks of leaves and secretory gland numbers of leafs of the taxa characterized by Hypericum calycinum L., Hypericum perforatum L., H. triquetrifolium Turra ve H. kazdaghensis Gemici  Leblebici collected from the surrounding of Bayramiç district of Çanakkale (NW Turkey) in years 2006 and 20...

In this paper, the wetland ecosystem rich in biological diversity in the Araplar Gorge area, which is located in the southeastern part of the Biga Peninsula in the northwestern Turkey, was studied on the basis of biological properties and environmental pollution parameters. The area shows a considerable biodiversity in terms of the communities of w...

New chorological data are presented for 108 species and subspecies from Bulgaria (records no. 46, 49, 55-62, 69-74, 83-108), Greece (14-43, 75-82) and Turkey-in-Europe (1-13; 44, 45, 47, 48, 50-54, 63-68). The taxa belong to the following families: Alismataceae (34), Amaryllidaceae (35), Apiaceae (16), Aristolochiaceae (17), Asclepiaceae (18), Aspl...

Bu çalışmada Çanakkale ili, Çan ilçesi ve kırsal çevresinde doğal yayılış göstermekte olan Orkide taksonları araştırılmıştır. Yörede gerçekleştirilen çalışmalarda ilçenin farklı yönlerinde yer alan değişik ekolojik özellikli lokalitelerinde 10 cinse ait (Anacamptis L.C.M. Richard, Cephalanthera L.C.M. Richard, Dactylorhiza Necker ex Nevski, Epipact...