K. G. Wiryawan

K. G. Wiryawan
IPB University | IPB · Department of Nutrition and Feed Technology

Professor
Isolation of cellulolytic degrading bacteria and fungi from Indonesian endemic herbivores

About

250
Publications
104,846
Reads
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1,319
Citations
Introduction
K. G. Wiryawan currently works at the Department of Nutrition and Feed Technology, Bogor Agricultural University. K. does research in Microbiology. Their current project is 'Isolation of rumen anaerobic fungi from Indonesian buffaloes and isolation of cellulolytic bacteria from several endemic herbivores of Indonesia’
Additional affiliations
July 1987 - present
IPB University
Position
  • Lecturer
Description
  • Ruminant Nutrition Nutrition Microbiology Reproductive Nutrition Probiotics and Prebiotics
March 1987 - present
IPB University
Position
  • Professor (Full)
Education
February 1989 - April 1994
University of Adelaide
Field of study
  • Animal Science
July 1981 - December 1985
IPB University
Field of study
  • Animal Production

Publications

Publications (250)
Article
Full-text available
Cellulolytic bacterial play an important role in the synthesis of microbial protein in the small intestine as high-quality protein. This research aimed to observe the growth patterns of cellulolytic bacterial isolates from the feces of endemic herbivorous animals in Indonesia, and to evaluate the effect of the addition of cellulolytic bacterial iso...
Article
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The study aimed to to evaluate the effect of feeding protected maggot oil at different levels on the ram sperm quality. The study used 15 local rams with an age of approximately 10–12 months and an initial weight of 19.99 ± 3.97 kg. The feeding rate was 4% of body weight per day. Feed was given 3 times a day, specifically in the morning (08.00 WIB)...
Article
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Fermented sausage is produced from beef and jack bean flour. Lactobacillus plantarum IIA-1A5 was employed as a starter culture. This study aimed to assess the organoleptic properties and physical attributes of fermented sausages. Three replications of a completely randomized design were employed in this study. Fermented sausages were divided into t...
Article
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Tannins are bioactive compounds found in plants and are very prospective for use in reducing methane gas production by ruminants. The effect of extracts of tannin compounds on methane gas parameters effectively reduces methane production. However, the population dynamics of the rumen microbiome showed varying results depending on tannin levels, typ...
Article
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Lactic acid bacteria as probiotic candidates can be isolated from black soldier fly larvae. This study aimed to develop encapsulated lactic acid bacteria (LAB) probiotics from BSF larvae and to evaluate the effect of encapsulation on probiotic viability during 12 weeks of storage at room temperature and 4°C. The experimental design was a factorial...
Article
Full-text available
Agustina S, Wiryawan KG, Suharti S, Meryandini A. 2024. The addition of anaerobic fungi isolates from buffalo rumen to increase fiber digestibility, fermentation, and microbial population in ruminants. Biodiversitas 25: 107-115. Rumen microbes have an important role in the rumen. Anaerobic fungi are microbes needed in the forage digestion process i...
Preprint
Full-text available
Black soldier fly maggot oil has emerged as a potential alternative energy source for livestock because of its high-fat content, which contains lauric, myristic, oleic, and palmitic acids that are known to improve livestock reproduction. A study was conducted to evaluate the effect of feeding protected maggot oil at different levels on the macrosco...
Article
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This research aimed to investigate the use of essential oils (EO) in vivo as a feed additive on rumen fermentation characteristics and methane gas through a meta-analysis approach by integrating various related studies. The data analyzed came from 21 journals, 26 studies, and 73 data points from 2007–2021, which were tabulated using Microsoft Excel...
Article
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The aim of this study was to evaluate the effect of diet containing fermented Arenga pinnata by-product on milk yield, dry matter intake, organic matter, and feed efficiency of lactating dairy cow. This research was conducted using Latin Square design 4 x 4, 4 treatments in 4 periods. The treatments were FA-0: basic diet (65% forage +19% tofu by-pr...
Article
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Marine macroalgae are the prospective and promising Indonesian natural resources containing bioactive compounds. This study aimed at evaluating various seaweed species with optimal biological activity to mitigate in vitro rumen methane emission and to confirm their antioxidant properties. In addition, relationships among several phenolic fractions...
Article
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This study aimed to evaluate the integration potential of cassava plants with goats in aerial production and goat manure-derived fertilizers. This research was conducted by purposive sampling with the consideration that Lampung Province is the central region of cassava production in Indonesia and is the region with the highest population of goats o...
Article
Full-text available
Suharti S, Novrariani N, Wiryawan KG. 2023. Short Communication: Morphological, biochemical, and molecular identification of cellulolytic bacteria isolated from feces of endemic tropical herbivores. Biodiversitas 24: 4046-4051. Indonesia has endemic herbivores that consume lignocellulose feedstuffs including grass, tree foliage, rice straw, and leg...
Article
Full-text available
Essential oils (EOs) are secondary metabolites of plants with antimicrobial functions that can be used as silage additives, but their effectiveness has been inconsistent. Therefore, this study aimed to evaluate the effects of EOs as silage additives on nutritional quality, fermentative products, microbial population, and in vitro rumen fermentation...
Article
Full-text available
Essential oils are plant secondary metabolites that have potential as antimicrobial agents. The use of essential oils as silage additives is expected to maintain feed quality and prevent the growth of spoilage microbes during ensiling. This meta-analysis study aimed to determine the effect of levels of essential oils as silage additives on chemical...
Article
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Background and aim: Resistant starch (RS) is difficult to digest in the digestive tract. This study aimed to evaluate the effects of heat-moisture treatment (HMT) on RS in cassava and examined its impact on rumen fermentation. Materials and methods: Cassava flour was used as a raw material and used in a randomized block design with four differen...
Article
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Objective: This study was conducted to evaluate the use of Megasphaera elsdenii as a probiotic on rumen fermentation, production performance, carcass traits and health of ruminants by integrating data from various related studies using meta-analysis. Methods: A total of 32 studies (consisted of 136 data points) were obtained and integrated into...
Article
Full-text available
Agustina S, Wiryawan KG, Suharti S, Meryandini A. 2022. Molecular identification of anaerobic fungi isolated from buffalo rumen with their growth rate, cellulase enzyme activity, and fermentation products characteristics. Biodiversitas 23: 6448-6455. Anaerobic fungi are essential microbes in the degradation process of feed fiber in the rumen with t...
Article
Full-text available
Urea has been utilized as a non-protein nitrogen supplement for ruminants due to its affordable price, abundantly available, easy to use, and positively affect microbial protein synthesis in the rumen. However, urea have a weakness since it is rapidly converted into ammonia in the rumen and may lead to nitrogen loss and even ammonia toxicity. Devel...
Article
The use of soy bean waste is an alternative roughage feed for local cattle fattening based on concentrate, particularly in the dry season. This study aimed to examine the productivity and income over feed cost of PO cattle fattened using ration containing soybeans pod for 100 days. Twelve male PO cattle with an average age of 18 months and an initi...
Article
Full-text available
Methane emission from ruminants can be mitigated with supplementation of medium chain fatty acids (MCFA) such as lauric acid that present in coconut oil. However, the effects of coconut oil supplementation on in vitro rumen fermentation and methane emission have been varied. This study was conducted to examine the effect of dietary coconut oil supp...
Article
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Palm oil is an abundant source of oil in Indonesia and some other tropical countries. This research aimed to investigate the effect of palm oil supplementation levels on in vitro rumen fermentability by integrating data from various related studies. The data used were 40 data points from 13 studies which reported different levels of palm oil supple...
Conference Paper
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Total mixed rations (TMR) containing conventional forage sorghum (Pahat), brown mid rib (BMR) forage sorghum (G5), or green midrib (GMR) forage sorghum (G8) were fed to local lambs to determine the effect on digestibility, performance and ruminal fermentation. Fifteen ewe lambs (aged < 1 year) with average live weight of 15±0.50 kg were assigned to...
Article
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Vinasse-molasses, the residue of condensed molasses is a co-product of sugar production. It is not reused in manufacturing process and become polluted. However, Its contents of 48-60% of sugar and high in potassium and sulphates. This study was to evaluate Vinasse-molasses utilization as a replacer of molasses in finishing ration on the growth perf...
Article
Desa Loyok berada pada jalur pariwisata Gunung Siu-Kotaraja yang banyak dikunjungi wisatawan lokal maupun mancanegara. Hal tersebut menyebabkan pengurus desa berharap Desa Loyok bisa lebih maju untuk mendukung program pariwisata dengan memiliki produk unggulan berupa daging dan telur itik. Selama ini usaha peternakan baik ternak besar dan kecil mas...
Article
Full-text available
Agustina S, Wiryawan KG, Suharti S, Meryandini A. 2021. The enrichment process and morphological identification of anaerobic fungi isolated from buffalo rumen. Biodiversitas 23: 469-477. Anaerobic fungi are one of the microbes that have an important role in rumen fiber degradation because they can produce cellulase enzymes and penetrate feed partic...
Article
Full-text available
Fajrih N, Wiryawan KG, Sumiati, Syahpura SK, Winarsih W. 2022. Identification of bioactive compounds of banana corm (Musa paradisiaca) using GC-MS and its inhibitory effect against pathogenic bacteria. Biodiversitas 23: 195-204. The banana corm is an agricultural waste that is abundant in nature but is rarely used even though it has many benefits i...
Article
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This paper is a review of some experiments using black soldier fly (BSF) and its by-product to explore their nutritional value, production potential in Indonesia and its application in the ration of ruminants. Evaluation on the effect of milk replacer, creep feed containing BSF, BSF frass and the possibility to use lactic acid bacteria from BSF as...
Article
Full-text available
This paper is a review of some experiments using black soldier fly (BSF) and its by-product to explore their nutritional value, production potential in Indonesia and its application in the ration of ruminants. Evaluation on the effect of milk replacer, creep feed containing BSF, BSF frass and the possibility to use lactic acid bacteria from BSF as...
Article
Full-text available
The aim of this study was to determine the effect of coating urea by chitosan at graded levels on ammonia concentration and rumen fermentation in vitro. This study used Factorial Randomized Complete Block Design (RCBD) to test ammonia parameter and Randomized Complete Block Design (RCBD) for pH, microbial protein synthesis, dry matter and organic m...
Article
Full-text available
This study aimed to evaluate the performance and fermentation characteristics in the rumen of Garut grade sheep fed bitter cassava (M. esculenta Crantz) leaf meal (BCL) supplemented with cyanide-degrading bacteria (CDB). Fifteen sheep (12 mo with average weight of 18.8 ± 1.02 kg) were placed in individual cages provided experimental diets and free...
Article
Full-text available
Urea is a source of Non-Protein Nitrogen (NPN). The utilization of urea in the ration is useful for increasing digestibility, dry matter intake, and increasing protein content. This study aims to compare the utilization of conventional urea and Slow Release Urea (SRU) to reduce ammonia concentration by in vivo study using meta-analysis. A total of...
Article
Full-text available
This study evaluated the effects of supplementation M. elsdenii on rumen fermentation and lactic acid concentration in vivo by integrating data from various related studies using a meta-analysis. A database was developed by integrating data from 72 treatments that originated from 17 articles. The parameters integrated were lactic acid concentration...
Article
Full-text available
The demand for high quality and healthy meat has been continuously increasing both in national and international markets. The objective of this study was to investigate the effect of gambir ( Uncaria gambir ) leaf extract as a feed additive on cholesterol content and consumer preference of goat meat. Eighteen Boerka goats aged 10-11 months with an...
Article
Full-text available
The purpose of this study was to examine the influence of aflatoxins on rumen fermentation in vitro and obtained rumen bacterial isolates capable of binding aflatoxin. This trial consisted of three stages. The first trial was a reduction of aflatoxin in the rumen in vitro. The second experiment was the isolation and characterization of rumen bacter...
Article
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Black seed or black cumin has the scientific name Nigella sativa (NS) is an herbal plant that is widely used to treat various diseases. The extraction of oil from NS seeds produces a byproduct called NS waste. This study aims to evaluate the physicochemical properties of products and NS waste produced by the cold press method. Analysis of physical...
Article
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Background and aim: Flushing with the manipulation of fatty acids, particularly polyunsaturated fatty acids, like linoleic and α-linolenic acids in the ration, is a strategy to raise the nutritional status of the female mammals to improve ovarian function and follicle development. This study was designed to investigate the effectiveness of flushin...
Article
Full-text available
Forage is a feed source for ruminant livestock, but one of the limiting factors of forage is high content of fiber in old forage plants. The fiber content in forage can only be degraded by rumen microbes. One of the rumen microbes that has fiber degrading activity is rumen anaerobic fungi because it can produce very active enzymes to degrade lignoc...
Article
Full-text available
ABSTRAK Saenab A, Wiryawan KG, Retnani Y, Wina E. 2020. Pengaruh sinergistik biofat dan biochar cangkang biji mete untuk mitigasi metana dalam rumen. JITV 25(3): 139-146. DOI: http://dx.doi.org/10.14334/jitv.v24i3.2475 Salah satu cara untuk mengurangi emisi metana adalah dengan menggunakan aditif pakan yang berasal dari ekstrak tumbuhan yang mengan...
Article
Full-text available
The effect of substituting native grass with jengkol (Archidendron jiringa) by-product on fermentation characteristics, rumen microbial profile, methane production, and hydrogen balance using in vitro method was investigated. Seven treatments (different composition of native grass, jengkol peel, jengkol leaves, and concentrate) with five replicatio...
Article
Full-text available
Forage is a feed source for ruminant livestock, but one of the limiting factors of forage is high content of fiber in old forage plants. The fiber content in forage can only be degraded by rumen microbes. One of the rumen microbes that has fiber degrading activity is rumen anaerobic fungi because it can produce very active enzymes to degrade lignoc...
Article
Full-text available
The study aimed to evaluate the effectiveness of a combination of biofat with biochar or biosmoke (bioindustrial products of cashew nut shells) at the best level as feed additive in reducing methane production and improving in vitro rumen fermentation. This experiment had two series of combination and each used a randomized block design with 6 trea...
Article
Full-text available
The effect of substitution native grass with jengkol ( A. jiringa ) peel on sheep performance was investigated. Three treatments substitution of jengkol peel (0, 15, 22.5%) were five replications in a randomized block design. This research used 15 sheep with body weight around 15-27 kg. The jengkol peel and concentrate were given in pellet form to...
Article
Full-text available
The in vitro experiment was aimed to evaluate the effect of soybean oil calcium soap (CaS-soybean) supplementation in ration on fermentability characteristics, microbial population and nutrient digestibility by using rumen fluid of Bali cattle. The experiment was arranged in a completelyrandomized block design with 4 different ration treatments wit...
Article
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Physiological stress occurs in does immediately after parturition. This study evaluated the blood hematology profile at postpartum in Ettawa grade does fed with different fatty acid flushing diets during the late gestation period and different litter sizes (LS). A total of 15 Ettawa grade does with a gestational age of 4 months were used in this st...
Article
Full-text available
There is not much information regarding the fiber profile from G5 and G8. The profile of the fiber associated with neutral detergent fiber (NDF) and acid detergent fiber (ADF) will affect the digestibility of sorghum mutant lines. The objectives of this research were to: 1) evaluate the profile of fiber fraction and estimate the relative feed value...
Article
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This study aims to examine the effect of feeding graded levels of fermented duckweed meal (FDM) in quail diets on production performances, egg internal quality and yolk cholesterol. A total of 100 two- months-old of quail layers ( Coturnix coturnix japonica ) were randomly allocated into four dietary treatments, in which each treatment consisted of...
Article
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DOI: 10.5398/tasj.2019.42.3.fm.i
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Article
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ABSTRAK Penelitian ini bertujuan untuk menganalisis pengaruh minyak kanola tanpa proteksi dan terenkapsulasi pada karakteristik fermentasi rumen. di domba garut jantan. Penelitian menggunakan rancangan acak kelompok (RAK) dengan rancangan 3 perlakuan dan 5 kelompok sebagai ulangan. Perlakuan terdiri dari P0 (kontrol/pakan ternak:konsentrat = 60:40)...
Article
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ABSTRAK Penelitian ini bertujuan untuk menyelidiki pengaruh dari pemberian pakan yang mengandung mikroenkapsulasi minyak kanola pada konsumsi, kecernaan nutrien, dan performa pertumbuhan pada domba. Lima belas domba garut jantan dengan rataan bobot badan awal 20.17 ± 4.65 kg digunakan dalam rancangan acak kelompok (RAK) pada periode pemeliharaan se...
Article
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G5 and G8 are two sorghum mutant lines projected as special sorghum varieties for forage in Indonesia. The objective of this research was to investigate the nutrient profile and in vitro gas production characteristics from two sorghum mutant lines as forage for ruminants. The two mutant lines were compared with Numbu and Pahat variety. Nutients and...
Article
Full-text available
This research was conducted to determine the interaction of NDF level and fiber particle size on the performance of production, digestibility of dry matter, crude fiber and fiber fraction, digestive tract, microbial profiles, volatile fatty acids (VFA), and total organic acid of rabbits during the fattening period. The experiment utilized 144 New Z...
Article
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DOI: https://doi.org/10.5398/tasj.2020.43.3.fm.i
Article
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G5 and G8 are two sorghum mutant lines projected as sorghum varieties for ruminant’s feed. However, most of the sorghum panicles contains phenolic compounds, especially in the form of condensed tannins. The objective of this research was to investigate the phenolic and tannin compounds from the panicles of two sorghum mutant lines. The second objec...
Article
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The use of antibiotics as a growth promoter for chickens may develop resistance to pathogenic bacteria in the digestive tract of chickens. Other negative impacts on food safety came from antibiotic residues. One alternative that can be used as a substitute for antibiotics is probiotics. Probiotics derived from lactic acid bacteria (LAB) is safe bes...
Article
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Hidayah N, Lubis R, Wiryawan KG, Suharti S. 2019. Phenotypic identification, nutrients content, bioactive compounds of two jengkol (Archidendron jiringa) varieties from Bengkulu, Indonesia and their potentials as ruminant feed. Biodiversitas 20: 1671-1680. Agricultural waste is abundant in tropical countries. Many farmers in these countries have be...
Article
Putak is a local feed in west Timor, East Nusa Tenggara Province. It is obtained from soft core of palm tree which is called gebang tree (Corypha gebanga). Putak contains high carbohydrate and fiber but low in protein. As an effort to increase the ptotein level in putak a research was conducted by fermenting it with mixed-cultured of Trichoderma re...