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February 2006 - present
Publications
Publications (24)
Background. Medicinal plant extracts are widely used in traditional and non-conventional medicine. Special interest is given to extracts obtained from transgenic so-called "hairy" roots. Usually, such roots are characterized by a high content of biologically active components. However, such plant transformation can lead to the appearance of both un...
Methylsensitive comet electrophoresis is based on the assessment of the level of DNA migration from individual lysed cells after treatment with methylsensitive restriction enzymes. Using model human lymphocytes, the optimal combination of restriction intensity and electrophoresis time was selected and a new approach for evaluating the relative leve...
At higher order levels chromatin is organized into loops, and this looping plays an important role in transcription regulation. In our previous works we investigated the kinetics of DNA loop migration during single cell gel electrophoresis (the comet assay) of nucleoids obtained from lysed cells. It was shown that there are three parts of DNA in nu...
Background:
The loop domain organization of chromatin, which plays an important role in transcription regulation, may depend on the cell functional state. The aim of this work was to investigate DNA loop reorganization upon functional transitions in two cell lines ‒ glioblastoma multiforme T98G and glioblastoma astrocytoma U373.
Materials and met...
Chromatin loops are important elements of both chromatin higher-order structure and transcription regulation system. Our previous works have shown that several features of the loop domain organization could be investigated by single cell gel electrophoresis (the comet assay) using the kinetic approach. In this study we applied this technique to stu...
The loop domain organization of chromatin, which plays an important role in transcription regulation, may depend on the cell functional state. The aim of this work was to investigate DNA loop reorganization upon functional transitions in the glioblastoma T98G cells. Methods. Single cell gel electrophoresis (a comet assay) was used to analyze the ki...
Chromatin structure at high levels of its organization, which remains not to be completely understood, attracts much attention because it is the basis of regulation of functional processes in nuclei of eukaryotic cells. An important aspect of this organization is the existence of relatively autonomic structural elements, the chromatin loop domains....
The comet assay has proved itself to be not only a method of detection of DNA damages at the level of individual cells but also an approach for investigation of spatial organization of DNA loop domains in nucleoids. Usually, those nucleoids are obtained after cell lysis in high-salt buffer (e. g. 2.5 M NaCl) with a detergent: these conditions ensur...
The comet assay is a sensitive method to assess DNA damages in single cells. The approach consists of an analysis of electrophoretic migration of DNA from nucleoids obtained after cell lysis in a thin layer of agarose. Although the method is widely used the physical mechanisms of DNA track formation remained to be rather elusive for a long time. Th...
The self-organization of C60 fullerene and cisplatin in aqueous solution was investigated using the computer simulation, dynamic light scattering and atomic force microscopy techniques. The results evidence the complexation between the two compounds. The genotoxicity of C60 fullerene, Cis and their complex was evaluated in vitro with the comet assa...
At higher order levels chromatin fibers in interphase nuclei are organized into loop domains. Gene regulatory elements (promoters and enhancers) are often located near the sites of loop attachments. Therefore, loop domains play a key role in regulation of cell transcriptional activity. We investigated the kinetics of DNA loop exit during single cel...
The loop domain organization of chromatin plays an important role in transcription regulation and thus may be assumed to vary in cells of different types. We investigated the kinetics of DNA loop migration during single cell gel electrophoresis (the comet assay) for nucleoids obtained from human lymphocytes, lymphoblasts and glioblastoma T98G cells...
The self-ordering of C60 fullerene, doxorubicin and their mixture precipitated from aqueous solutions was investigated using atomic-force microscopy. The results suggest the complexation between the two compounds. The genotoxicity of doxorubicin in complex with C60 fullerene (C 60+Dox) was evaluated in vitro with comet assay using human lymphocytes...
At higher order levels chromatin is organized into loops that appear as a result of contacts between distant
loci. The aim of this work was to investigate the length distribution of the DNA loops in nucleoids obtained
after lysis of either whole cells or isolated cell nuclei. Methods. We used single cell gel electrophoresis
to analyze the kinetics...
The self-ordering of C60 fullerene, doxorubicin and their mixture precipitated from aqueous solutions was investigated using atomic-force microscopy. The results suggest the complexation between the two compounds. The genotoxicity of doxorubicin in complex with C60 fullerene (С60+Dox) was evaluated in vitro with comet assay using human lymphocytes....
Single-cell gel electrophoresis, or the comet assay, is usually performed with nucleoids prepared after a lysis of either whole cells (more often) or isolated cell nuclei (rarely). Electrophoretic properties of the second type of nucleoids have never been investigated carefully. We measured the kinetics of the DNA exit from nuclei-derived nucleoids...
At higher order levels chromatin is organized into loops. This looping, which plays an important role in transcription regulation and other processes, remains poorly understood. We investigated the kinetics of DNA loop migration during single cell gel electrophoresis (the comet assay). The migration of a part of the loops was shown to be reversible...
In single-cell gel electrophoresis (the comet assay) the DNA of lysed cells, the nucleoids, extends towards the anode in a track resembling a comet tail. The aim of this work was to investigate the effects of changes in DNA topology on this process. Methods. We used the kinetic approach, proposed earlier by us, to measure a relative amount of DNA i...
We investigated the mechanisms of DNA exit during single-cell gel electrophoresis (the comet assay) by measuring the kinetics of the comet tail formation. In the neutral comet assay, the rate of DNA exit was found to be dependent on the topological state of DNA, which was influenced by either ethidium bromide or a low radiation dose. The results cl...
Results of the kinetics of DNA exit in neutral and alkaline variants of single cell gel electrophoresis are compared. It is
shown that preincubation of samples in an alkaline buffer makes DNA exit in neutral electrophoresis impossible and, in contrast,
effectively facilitates it within such buffers. We conclude that the alkali disrupts interactions...
The kinetics of DNA exit during single cell gel electrophoresis (comet assay) of human lymphocytes has been investigated. The electrophoresis was carried out at neutral pH either in the absence or in the presence of different ethidium bromide concentrations. The demonstrated dependence of the DNA exit efficiency on the ethidium bromide concentratio...
Aim. Aim was to investigate possible changes in the DNA loop domain organization upon activation of human lymphocytes. The rational for this task is the knowledge that the chromatin looping plays an important role in transcription regulation and thus may vary depending on cell functional state. Methods. The kinetics of DNA loop migration during sin...
