Jutta Wirth

Jutta Wirth
Hochschule Rhein-Waal

PhD & master in biology
Scientist Rhine-Waal University of Applied Sciences URL: https://www.hochschule-rhein-waal.de/de/seite/dr-jutta-wirth

About

50
Publications
6,635
Reads
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3,060
Citations
Introduction
Molecular biology, biology teacher, 3D printing and laser cutting seminars, practical courses for 3D printing, Cell Culture Techniques, Laboratory research and development, Laser cutting, professional training courses, Real-Time PCR, Primer Design, Cell culture and techniques, primary cell culture, stem cells, In vitro tests, non-animal test methods, test tube experiments, DNA Fingerprint, Mutation analyses, in vitro techniques, next generation sequencing.
Additional affiliations
January 2015 - July 2020
Wageningen University & Research and Biobioseminars
Position
  • Instructor
June 2010 - June 2011
BLGG Research BV
Position
  • Senior Researcher
Description
  • Senior researcher for the agricultural crop industry, development of SRYRgreen based quantitative Real Time PCR applications for the detection of multiple plant pathogens.
January 2010 - July 2010
Wageningen University & Research
Position
  • Lecturer
Description
  • The work focused on the dissemination of the IMAQUANIM project, primarily addressed the scientific community by means of publications in scientific journals and building up a communication structure and strategy with the project partners.
Education
April 1992 - December 1995
University of Freiburg
Field of study
  • Human Genetics
October 1983 - June 1991
Freie Universität Berlin
Field of study
  • Biology

Publications

Publications (50)
Article
Full-text available
We describe two unrelated patients with cytogenetically visible deletions of 21q22.2-q22.3 and mild phenotypes. Both patients presented minor dysmorphic features including thin marfanoid build, facial asymmetry, downward-slanting palpebral fissures, depressed nasal bridge, small nose with bulbous tip, and mild mental retardation (MR). FISH and mole...
Conference Paper
Full-text available
Escaping The Company Bubble To Innovate: A Cross Innovation Model In this research, we described how cross innovation can systematically be integrated into small and medium-sized enterprises (SME´s). Based on a profound literature research specified on cross innovation and innovation management in more general (processes and management activities),...
Conference Paper
In this research we described how cross innovation (short -XI) can systematically be integrated into small and medium-sized enterprises (SME´s).• Design/methodology/approach– Based on a profound literature research specified on XI and innovation management in more general (processes and management activities) we developed a model for XI, which allo...
Article
Full-text available
Most birds engage in extra-pair copulations despite great differences across and within species. Besides cost and benefit considerations of the two sex environmental factors have been found to alter mating strategies within or between populations and/or over time. For socially monogamous species, the main advantage that females might gain from mati...
Article
Full-text available
3D bio printing is used for the production of artificial organs. A Computer Aided Design (CAD) is used to visualize the specifications and patterns of the three-dimensional organ. In this way various organs systems can be engineered as three-dimensional (3-D) structures. The 3D bio printing technique allows to combine various cells and biomaterials...
Article
Full-text available
BIG Science Data, computergestützte Lösungen zur Textanalyse, Erkenntnisgewinn in der Gesundheitsmedizin
Article
Full-text available
Genetic disorders are common in dogs and in the media it is reported that genetic disorders are more frequent in pedigree dogs than in look-a-likes or in mixed-breed dogs. Here, we consider pedigree dogs as purebred dogs (i.e. matching a breed-specific morphology) with a registered and certified pedigree, whereas look-a-likes dogs are dogs without...
Article
Full-text available
Dass für den Mensch bestimmte Bakterien und Viren, die über Nahrungsmittel verbreitet werden, auch krankmachend und lebensbedrohlich sein können, hat sich in letzter Zeit häufig bestätigt. Erst letztes Jahr sind auf einem Hotelschiff Noroviren als gefährliche Bedrohung für die Gesundheit aufgetreten. 70 Menschen wurden plötzlich mit Noroviren auf e...
Article
Full-text available
Parathyroid hormone-like hormone (PTHLH) is an important chondrogenic regulator; however, the gene has not been directly linked to human disease. We studied a family with autosomal-dominant Brachydactyly Type E (BDE) and identified a t(8;12)(q13;p11.2) translocation with breakpoints (BPs) upstream of PTHLH on chromosome 12p11.2 and a disrupted KCNB...
Article
We report on the characterization of a de novo, apparently balanced translocation t(X;15)(p11.3;q26) detected in a girl with multiple congenital malformations. Replication banding studies on Epstein-Barr virus transformed peripheral blood lymphocytes revealed non-random X chromosome inactivation with predominant inactivation of the derivative X chr...
Article
Full-text available
Hereditary ataxia is a clinically and genetically heterogenous group of disorders. Most are progressive and associated with other neurological abnormalities. Early onset, non-progressive cerebellar ataxia (OMIM #117360) has been described as a dominantly inherited disorder associated with isolated vermal atrophy1–3 or generalised atrophy of the cer...
Chapter
Parathyroid hormone-like hormone (PTHLH) is an important chondrogenic regulator; however, the gene has not been directly linked to human disease. We studied a family with autosomal-dominant Brachydactyly Type E (BDE) and identified a t(8;12)(q13;p11.2) translocation with breakpoints (BPs) upstream of PTHLH on chromosome 12p11.2 and a disrupted KCNB...
Article
Full-text available
We report on the cytogenetic and molecular characterisation of a constitutional de novo interstitial deletion of chromosome 22q12.1-q12.3 in a dysmorphic girl. The deletion extends over approximately 8 Mb including the NF2 gene region. The corresponding deletion syndrome is characterised by severe developmental delay accompanied by multiple malform...
Article
We report the results of detailed clinical and molecular-cytogenetic studies in seven patients with ring chromosome 18. Classical cytogenetics and fluorescence in situ hybridization (FISH) analysis with the chromosome 18 painting probe identified five non-mosaic and two complex mosaic 46,XX,dup(18)(p11.2)/47,XX,dup(18)(p11.2),+r(18) and 46,XX,dup(1...
Article
We report the results of detailed clinical and molecular-cytogenetic studies in seven patients with ring chromosome 18. Classical cytogenetics and fluorescence in situ hybridization (FISH) analysis with the chromosome 18 painting probe identified five non-mosaic and two complex mosaic 46,XX,dup(18)(p11.2)/47,XX,dup(18)(p11.2),+r(18) and 46,XX,dup(1...
Article
Full-text available
We report on the molecular characterization of a translocation t(1;19)(q21.3;q13.2) in a female with mental retardation, ataxia and atrophy of the brain. Sequence analysis of the breakpoints revealed an ALU:-repeat-mediated mechanism of recombination that led to truncation of two genes: the kinase CLK2 and PAFAH1B3, the gene product of which intera...
Article
Variation of signals is a widespread feature in animal communication. Signals usually vary among signallers and in some cases vary across geographic areas. Individual variation provides the basis for individual recognition and thus has important implications for social interactions. Microgeographic variation can reveal insights into patterns of dis...
Article
Full-text available
Editor—Carriers of de novo balanced reciprocal translocations and inversions have an increased risk of approximately 6% for developing multiple congenital abnormalities (MCA) and/or mental retardation (MR), compared to a 2-3% risk overall in newborn populations.1 2 Cytogenetically cryptic deletions or physical disruption or inactivation of a gene(s...
Article
We describe a three-year-old girl with a triangular face, epicanthus, midfacial hypoplasia, apparently low-set ears, a small mouth with thin vermilion border, and a small chin, hypermobile joints, developmental delay with insecure gait, dystonic movement disorder, speech defect, and a history of unexplained undernutrition. She has a de novo, appare...
Article
Full-text available
Balanced translocations affecting the paternal copy of 15q11–q13 are a rare cause of Prader–Willi syndrome (PWS) or PWS-like features. Here we report on the cytogenetic and molecular characterization of a de novo balanced reciprocal translocation t(X;15)(q28;q12) in a female patient with atypical PWS. The translocation breakpoints in this patient a...
Article
We describe a three-year-old girl with a triangular face, epicanthus, midfacial hypoplasia, apparently low-set ears, a small mouth with thin vermilion border, and a small chin, hypermobile joints, developmental delay with insecure gait, dystonic movement disorder, speech defect, and a history of unexplained undernutrition. She has a de novo, appare...
Article
Full-text available
A sensitive technique is needed for screening whole genome imbalances in dyschromosomal patients when G-banding shows normal karyotypes or apparently balanced translocations. In this study we performed highly sensitive comparative genomic hybridisation analysis on a number of such cases and revealed chromosomal imbalances in all.
Article
Glomerulopathy with fibronectin deposits (GFND, MIM 601894) is an autosomal dominant kidney disease that leads to terminal renal failure at a median age of 47 years. It represents a distinct entity of membranoproliferative glomerulonephritis (MPGN) type III and is characterized by the unique feature of massive glomerular deposits of fibronectin. We...
Article
Full-text available
Partial trisomy 9q represents a rare and heterogeneous group of chromosomal aberrations characterised by various clinical features including pyloric stenosis. Here, we describe the case of a 1 year old female patient with different dysmorphic features including pyloric stenosis and prenatally detected partial trisomy 9q. This partial trisomy 9q has...
Article
Full-text available
Mental retardation is a very common and extremely heterogeneous disorder that affects about 3% of the human population. Its molecular basis is largely unknown, but many loci have been mapped to the X chromosome. We report on two mentally retarded females with X;autosome translocations and breakpoints in Xp11, viz., t(X;17)(p11;p13) and t(X;20)(p11;...
Article
DXS6673E is a candidate gene for nonspecific X-linked mental retardation and encodes a novel Zn-finger protein. The ortholog murine gene DXHXS6673E in XC-D was isolated and characterized. It is ubiquitously expressed in all embryonic stages and adult tissues. Two different transcription start sites exist that result in two major transcripts of 6055...
Article
Full-text available
Disease associated balanced chromosome rearrangements (DBCRs) have been instrumental in the isolation of many disease genes. To facilitate the molecular cytogenetic characterisation of DBCRs, we have generated a set of >1200 non-chimeric, cytogenetically and genetically anchored CEPH YACs, on average one per 3 cM, spaced over the entire human genom...
Article
Full-text available
The nail patella syndrome (NPS1) is an autosomal dominant disorder characterised by dysplasia of the finger nails and skeletal abnormalities. NPS1 has been mapped to 9q34, to a 1 cM interval between D9S315 and the adenylate kinase gene (AK1). We have mapped the breakpoints within the candidate NPS1 region in two unrelated patients with balanced tra...
Article
Dac is a novel nuclear factor in mouse and humans that shares homology with Drosophila dachshund (dac). Alignment with available sequences defines a conserved box of 117 amino acids that shares weak homology with the proto-oncogene Ski and Sno. Dac expression is found in various neuroectodermal and mesenchymal tissues. At early developmental stages...
Article
Full-text available
A 39 year old male with primary infertility was diagnosed as having Klinefelter syndrome by conventional cytogenetic analysis, which also showed an abnormal chromosome 12. Fluorescence in situ hybridisation (FISH) analysis of the aberrant chromosome using a 12 specific centromeric probe showed a break in the alphoid repeats followed by an inversion...
Article
Full-text available
Nail patella syndrome (NPS) is an autosomal dominant disorder characterized by dysplasia of the nails and patella, decreased mobility of the elbow, iliac horns and in some cases nephropathy. Linkage studies have localized the NPS locus to chromosome 9q34 within a 1-2 cM interval between D9S60 and the adenylate kinase gene (AK1), but the gene has re...
Article
Full-text available
The clinical features, airway histology, and detection of hexamethylene diisocyanate (HDI) protein adducts in endobronchial biopsies from a patient with HDI asthma are described. Isocyanate asthma was diagnosed by history, methacholine challenge, and workplace HDI challenge. Bronchoscopy was performed 24 h after challenge and immunohistochemical st...
Article
Full-text available
In the course of a research project aimed at the molecular characterization of balanced chromosome rearrangements associated with mental retardation (MR), several YACs spanning MR-associated chromosomal rearrangements in the 13q14-->q22 region were identified. To facilitate the search for relevant candidate genes, we have analyzed a total of 102 ES...
Article
We have generated a human subtelomere probe panel, utilizing well characterized CEPH YACs, for the investigation of human chromosome pathology and evolution through fluorescent in situ hybridization (FISH). Region-specific FISH probes will be extremely valuable for detecting cytogenetically cryptic telomere abnormalities. Here, we present the first...
Article
A somatic cell hybrid panel was constructed consisting of seven hybrids with translocation breakpoints spanning the region 17q23-->q25. Hybrid clones carrying the longarm derivative of chromosome 17 in the absence of the normal chromosome 17 and of the derivative 17 were initially identified by PCR typing for a proximal and distal 17q marker. The t...
Article
The recent long-term studies from England, France, and the Netherlands, as well as our own, indicate that transcatheter embolotherapy is definitive treatment for PAVM. More recently, Puskas et al have questioned transcatheter embolotherapy as a primary treatment for patients with PAVM (4,56). Their opinion was based on two recurrences among five pa...
Article
Full-text available
Campomelic dysplasia (CMPD1) and autosomal XY sex reversal (SRA1) are caused by mutations in the SRY-related gene SOX9 on 17q. Unexpectedly, the 17q breakpoints in four CMPD1 translocation cases previously analyzed by us and others map 50 kb or more from SOX9. Here, we present clinical, cytogenetic, and molecular data from a new CMPD1/SRA1 patient...
Article
SOX genes share a high sequence identity with the HMG box present in the testis determining gene SRY. We have identified a HMG box-like sequence motif on six contiguous cosmids, which cross-hybridize to a SOX9 cDNA probe. A data base search revealed a high similarity of the deduced amino acid sequence to the human SOX12 and the murine Sox16 HMG dom...
Article
Parathyroid hormone-like hormone (PTHLH) is an important chondrogenic regulator; however, the gene has not been directly linked to human disease. We studied a family with autosomal-dominant Brachydactyly Type E (BDE) and identified a t(8;12)(q13;p11.2) translocation with breakpoints (BPs) upstream of PTHLH on chromosome 12p11.2 and a disrupted KCNB...
Article
A human autosomal XY sex reversal locus, SRA1, associated with the skeletal malformation syndrome campomelic dysplasia (CMPD1), has been placed at distal 17q. The SOX9 gene, a positional candidate from the chromosomal location and expression pattern reported for mouse Sox9, was isolated and characterized. SOX9 encodes a putative transcription facto...
Article
The complete deduced amino acid sequence and mRNA expression of human unconventional myosin-IC (HuncM-IC) are described. Sequencing of overlapping cDNA clones reveals a message of 4666 nucleotides with a single open reading frame predicted to encode a 127 kDa protein of 1109 amino acids. HuncM-IC is composed of three discrete regions: a characteris...
Article
Myosin diversity in the human epithelial cell line Caco-2BBe, the porcine epithelial cell line LLC-PK1 (CL-4), human peripheral blood leukocytes, and human liver was analyzed. PCR amplification yielded 8-11 putative myosins (depending on the cDNA source) representing six distinct myosin classes. Analysis of clones obtained by hybridization screenin...

Questions

Questions (2)
Question
3D printing and applications in molecular biology, I would need good examples?
Question
Real time PCR and primer design with locked nucleic acids (LNAs).
How is the specificity of the qPCR and quality compared to LNAs?

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Projects

Projects (7)
Project
Voronoi patterns and voronoi designs Making organic looking models we add varanoi paaterns to a STL file or 3D model and print them with a 3D printer. We also can lasercut the Voronoi patterns out of wood or other material to design a product.