Juraj Pistl

• University of Veterinary Medicine in Košice

The present study reports on serosurvey on the tick-borne encephalitis virus European subtype (TBEV; genus Flavivirus), and the tick-borne Kemerovo (KEMV) and Tribeč (TRBV) orbivirus (genus Orbivirus) infections in tick-infested and non-infested birds. No virus RNA was detected in the blood clots. Birds were infested mostly by Ixodes ricinus, but H...

West Nile virus (WNV) is a mosquito-borne flavivirus (the genus Flavivirus) representing a medical and veterinary public health concern. Birds are the most important reservoirs. Culicine mosquitoes transmit WNV to vertebrate hosts (including horses and humans) and migratory birds play role in its long-distance transport. Slovakia is geographically...

Probiotics are beneficial microorganisms, defined as "live microorganisms which, when administered in a reasonable amount, provide health benefits for the host". Not only the types of bacteria themselves, but also various other factors such as the ability to produce exopolysaccharides, biofilms, etc. significantly affect the positive effect of prob...

In our previous study we confirmed an antiviral activity of probiotic Lactobacillus reuteri L26 which was mediated by stimulation of local intestinal immunity. The aim of this paper was to evaluate the influence of L. reuteri L26 on the systemic immune response in gnotobiotic mice infected with porcine circovirus type 2 (PCV2). A total of 30 germ-f...

FISH (fluorescence in situ hybridization) analysis of the intestinal tract of conventional mice, following 14-day supplementation of feed with host non-specific (porcine) strain L. reuteri L2/6, showed in the presence of complex microbiota, a significant increase in the counts of representatives of the genera Lactobacillus and Bifidobacterium, and...

The tick-borne encephalitis virus (TBEV) and West Nile virus (WNV) are arboviruses of the genus Flavivirus in the family Flaviviridae. Their hosts are vertebratesof which rodents are the reservoirs of TBEV and birds are the reservoirs of WNV. Both viruses are transmitted from reservoirs to mammals by vectors. TBEV is transmitted by ticks (mostly Ix...

Probiotic bacteria are frequently used for prevention of bacterial infections of the gastrointestinal tract, but there are only limited studies on their efficacy against viral gut infections in animals. The aim of this study was to investigate the effect of probiotic Lactobacillus reuteri L26 Biocenol(TM) on the innate and adaptive immune responses...

The presentation is about detection and isolation of TRBV from ticks after 50 years. The tick were collected from grazing goats. The new locality is different from the original one. Genetic analysis showed realtionship with the original strain from Slovakia and with two strains from Ukraine. A close relationship is with Lipovník virus. However, dee...

Tick-borne encephalitis virus (TBEV, Flaviviridae, Flavivirus) and Tribeč virus (TRBV, Reoviriae, Orbivirus) are arboviruses transmitted by ticks. Tribeč virus is a member of the Great Island virus serogroup. Both viruses cause serous meningoencephalitis. Here, we describe the occurrence of TRBV and TBEV in Ixodes ricinus in Dúbrava district (Prešo...

Tribeč virus (TRBV) together with Lipovník and Kemerovo viruses are members of the Great Island virus serogroup in the genus Orbivirus (Reoviridae). TRBV was first time isolated in Slovakia from nymphs of Ixodes ricinus collected in Tribeč Mountains, from rodents and sentinel goats. Herein, we focus on the prevalence of TRBV neutralizing antibodies...

West Nile virus (WNV) is a mosquito-borne neurotropic pathogen that presents a major public health concern. Information on WNV prevalence and circulation in Slovakia is insufficient. Oral and cloacal swabs and bird brain samples were tested for flavivirus RNA by RT-PCR using newly designed generic primers. The species designation was confirmed by s...

The study of biofilm function in vivo in various niches of the gastrointestinal tract (GIT) is rather limited. It is more frequently used in in vitro approaches, as an alternative to the studies focused on formation mechanisms and function of biofilms, which do not represent the actual in vivo complexity of microbial structures. Additionally, in vi...

In this study, the biofilm-forming potential of intestinal Lactobacillus reuteri strains under different culture conditions was characterized by microtiter plate biofilm assays. Moreover, the spatial organization of exogenously applied L.reuteri L2/6 (a pig isolate) at specific locations in gastrointestinal tract of monoassociated mice was investig...

BACKGROUND Anthocyanins, compounds that represent the major group of flavonoids in berries, are one of the most powerful natural antioxidants. The aim of this study was to evaluate biological activities and comparison of anthocyanin-rich extracts prepared from chokeberry (Aronia melanocarpa), elderberry (Sambucus nigra), bilberry (Vaccinium myrtill...

Despite a great interest in Malassezia yeasts their metabolism is still little known. Most studied is their lipid metabolism. Only recently it was found that Malassezia produces pigments derived from tryptophan, which play a role in the pathogenesis of skin diseases. Malassezin synergism with other indole pigments is considered to be the cause of c...

The aim of this study was to investigate the toxic effect of the metal salt cadmium chloride dihydrate on the rabbit kidney cell line using the xCELLigence system or real-time cell analyser (RTCA), and to compare this relatively new method with standard biological cytotoxicity assays. This system provides real-time monitoring of cell behaviour and...

Organophosphates are used frequently in Europe for pest control due to their low price and broad spectrum of activity. In 2003, they accounted for over 59% (4645 tonnes) of insecticide sales in the EU, with chlorpyrifos the top selling insecticide (15.6%, 1226 tonnes). Chlorpyrifos was also one of the most widely used organophosphates in the US for...

Abbreviations: ISH = in situ hybridisation; PCV-2 = porcine circovirus 2; PMWS = postweaning multisystemic wasting syndrome Porcine circovirus 2 (PCV-2) is a causative agent of several diseases of pigs that are collectively termed porcine circovirus diseases. The virus has a single-stranded DNA genome of a size approximately 1.8 kb, virions of 17–2...

This work focused on the isolation of thermophilic Campylobacter spp. in samples obtained from dog droppings. There were 135 samples collected and examined from both clinically healthy and diseased dogs from households, clinics, rehabilitation centres and dog shelters in eastern Slovakia. The isolation of the Campylobacter spp. was achieved by the...

Abstrakt Expresia domény III obalového proteínu vírusu západonílskej horúčky (West Nile virus) pomocou pQE-30 UA-mCherry-GFP vektora a jej využitie v detekcii WNV protilátok veterinárskeho lekárstva a farmácie v Košiciach, Košice 2 Neuroimunologický ústav SAV, Bratislava csank@uvlf.sk Vírus západonílskej horúčky (West Nile virus – WNV) je neurotróp...

In the present study three different cell cultures, derived from rabbit kidney (RK13), rat liver (WBF344) and insect origin (Sf21), were used to examine the cytotoxic effect of the insecticide endosulfan. Cytotoxicity was determined on the basis of cell proliferation activity; the cellular damage was assessed by evaluation of cytopathic effect and...

Porcine circovirus 2 (PCV-2) is a primary agent of post-weaning multi-systemic wasting syndrome (PMWS), ubiquitous in pig herds. The course of viraemia and seroconversion in naturally infected pigs were investigated in piglets from the 2nd week of their life. Piglets were divided into seropositive (Ab(+) ) and seronegative (Ab(-) ) groups. Subseque...

There is an increasing need for rapid and easily interpreted in vitro assays to screen for possible cytotoxicity of pesticides. The objective of this study was to investigate the effect of the carbamate insecticide bendiocarb on mammalian and insect cell cultures. The cytotoxicity of this insecticide was evaluated by cell proliferation and cellular...

We investigated the effects of dietary addition of sage extract on the biochemical parameters, weight of some body organs and changes in the counts of Salmonella Enteritidis PT4 (SE) in experimentally infected chickens. The following diets were used: basal diet, basal diet with addition of an extract of Salvia officinalis L. (S), basal diet and SE,...

In the present study four different cell cultures, derived from rabbit kidney (RK13), rat; and murine liver (WBF344 and Hepa 1c1c7) and insect origin (Sf21) were used to examine the effects of chlorpyrifos. Sf21 cells were the most sensitive to chlorpyrifos, with significant suppression of their proliferative activity ranging from 10(-1)-10(-5) M....

Dichlofluanid is a halogenated sulfonamide derivative from the group of aryl (dichlorofluoromethyltio) fungicides. It was used as a fungicide and acaricide in approved agricultural pesticides from 1965. Nowadays it is not included in any of the lists for European re-assessment of active ingredients under Directive 91/414/EEC. On the other hand, it...

We investigated the influence of administration of flax-seed oil on interaction of Lactobacillus plantarum - Biocenol™ LP96 and Escherichia coli O8:K88ab:H9 in the gut of germ-free piglets. When compared to animals supplemented with L. plantarum, the counts of lactobacilli in the jejunal and ileal mucosa and in the intestinal content were significa...

The protective effect of Enterococcus faecium EF55 in chickens challenged with Salmonella enterica serovar Enteritidis phage type 4 (SE PT4) was assessed. The antibacterial effect on the bacterial microflora in the small intestine in relation to white blood cell count, phenotyping of peripheral blood and intestinal lymphocytes, functional activity...

The effects of sage on the enteral microbiota and leukocytic response of chickens challenged with Salmonella Enteritidis (SE) PT4 were studied. Chickens were divided into four groups: control, sage (S), SE and sage+S. Enteritidis (SSE). Groups S and SSE were fed sage extract (8.2 g kg) for 21 days. Groups SE and SSE were challenged with SE (10 CFU)...

The effect of oregano essential oil on growth performance, intestinal alkaline phosphatase (IAP) level, enterocyte proliferative ability, plasma proteins, and plasma minerals (calcium and magnesium) were studied in 1 day-old Ross 308 hybrid broiler chickens under commercial conditions during 42 experiment. Chickens fed oregano oil supplemented diet...

The prevalence of porcine circovirus 2 (PCV-2) infection in the pig population in Slovakia was investigated. Sera from pigs suspected for post-weaning multisystemic wasting syndrome (PMWS) as well as clinically healthy pigs were tested for viral DNA and specific IgM and IgG antibodies. Pigs (n = 198) were categorized to weaning, grower and fattenin...

Supplementation of oregano and sage extracts into the feed of experimental chickens a caused significant increase of mitogen-activated lymphocyte proliferation on the seventh week of feeding. On the other hand, fed diet supplemented with both extracts did not change number of CD3, CD4, CD8, IgM and IgG positive peripheral blood cells examined by fl...

The effects of sage extract on the activity of intestinal alkaline phosphatase (AP), proliferative ability of enterocytes, and growth performance in chickens were studied during 42 days of the experiment under commercial conditions. A significant increase of body weight gain was found in chickens fed with sage extract in the grower period (17-29 da...

Letter to the Editor No abstract Keywords: Bovine viral diarrhea virus 1 and 2; cell line susceptibility; virus recovery.

Objective of this study was to investigate changes in biological activity of blood leukocytes in Japanese quails, caused by zinc and cadmium administration. Four groups of Japanese quails were used. Three experimental groups of quails were exposed either to Cd (0.12 mg Cd/quail), Zn (4 mg Zn/quail) or a combination of Cd and Zn (0.12 mg Cd, 4 mg Zn...

The present study investigated the effect of the probiotic strain Enterococcus faecium DSM 7134 (2 x 10(7) cfu per g of feed) (probiotic group), natural humic substances (7g x kg(-1) feed) (HS group), and the combination of both (probiotic + HS group) on the performance, serum biochemistry, and biological activity of leucocytes of broiler chickens....

According to world statistics, dogs and cats are the species that owners most frequently seek assistance with potential poisonings, accounting 95–98% of all reported animal cases. Exposures occur more commonly in the summer and in December that is associated with the holiday season. The majority (>90%) of animal poisonings are accidental and acute...

The protective effect of Enterococcus faecium EF 55 against Salmonella enterica serovar Enteritidis phage type 4 (SE PT4) was studied in 1-day-old chicks. The EF 55 strain (isolated and characterised by the authors earlier) was applied daily (1.10(9) CFU/0.2 ml PBS) for 7 days. Oral inoculation of the SE PT4 strain was performed on day 8 in a singl...

Circoviruses belong to the family of small DNA viruses, which infect a number of animal species. The most important circovirus in swine is porcine circovirus type 2 (PCV2). The most frequent clinical form of circovirus infection in swine is the post-weaning multisystemic wasting syndrome (PMWS). Because Slovakia is one of the countries with unknown...

Lead increasingly contributes to pollution of the environment and may play a role in the development of adverse effects in the human and animal body. Data concerning its mutagenic, clastogenic, and carcinogenic properties have been conflicting. In this study, we evaluated the frequency of micronuclei in bone marrow erythrocytes of rats treated with...

SPF male Wistar rats were exposed for four months to lead acetate trihydrate present in drinking water (100 mg/l) and subsequently infested with 1000 ± 100 infective A. suum eggs. Metabolic activity of phagocytes and proliferative activity of T-lymphocytes were investigated on Day 4 and 8 after A. suum eggs infestation (Day 130 and 134 of lead acet...

Six cows suffering from mastitis were treated intramuscularly after the morning milking with tylosin at a dose of 10 mg/kg b/w. for five consecutive days. The milk samples were collected just before the experiment (0 h), and 300 h (13 days) at 12-h intervals from the evening and morning milking. Staphylococcus aureus and pathogenic streptococci wer...

In this study pesticides with different chemical structures (dichlofluanid, endosulfan, simazine, tolylfluanid and triallate) were examined for their potential cytotoxic effect on proliferative activity of cell cultures of mammalian origin. Cell lines Madin-Darby Bovine Kidney (MDBK), Rabbit Kidney (RK13), Porcine Kidney (PK15), and semicontinual l...

We analyzed chromosome aberrations, micronucleus frequency, mitotic index (MI), and nuclear division index (NDI) in peripheral lymphocytes of sheep subchronically exposed to the fungicide Euparen Multi (containing 50% tolylfluanid). Euparen Multi was administered by rumen sonde to group of Merino sheep (seven sheep/group) at 93 mg/kg body weight (1...

Koãi‰ová A., J. Pistl, R. Link, E. âonková, M. Goldová: Maggot Debridement Therapy in the Treatment of Footrot and Foot Scald in Sheep. Acta Vet. Brno 2006, 75: 277-281. In sheep with acute and chronic interdigital skin inflammation, maggot therapy was used for the debridement and speeding up the healing process. Sheep were housed at the university...

The aim of the present study was to evaluate the influence of the probiotic preparation BioPlus 2B, based on Bacillus licheniformis and Bacillus subtilis, on daily weight gains, feed conversion, general health, and stimulation of phagocytosis and mitogenesis of lymphocytes in pigs. Administration of probiotics significantly affected body weight and...

The polymerase chain reaction (PCR) was used in combination with microbiology methods for identifying faecal bacteria. The changes were monitor in the bacterial flora of chicken's caecum after preventive peroral application of Enterococcus faecium EK13 and consecutive infection with Salmonella enterica subsp. Enteritidis.The preventive treatment wi...

The effect of subchronic exposure to fungicide tolylfluanid on humoral and cell-mediated immune parameters in Merino sheep was studied. Fungicide was administered by rumen tube at a daily dose of 1/20 LD 50 for 90 days. Both exposed and control animals were immunized with vaccine Bodibion inj. a.u.v., containing inactivated bacteria Coxiella burnet...

PCR analysis is regarded to be a quick and reliable method of Salmonella detection in food and animal samples. The mode of sampling and sample preparation influence the sensitivity and accuracy of the analysis. The 10 days old, clinically healthy Salmonella-free chickens were experimentally infected with Salmonella enterica subsp. Enteritidis strai...

The induction of micronuclei was evaluated in 24 Wistar rats (12 male and 12 female) after subchronic inhalation exposure to the mixture of benzene, cyclohexanone and cyclohexane, in the dose of 0.72 g/m 3 ; 5 d per week; for 105 d, against control group (6 male and 6 female). In the exposed group of females the frequency of micronucleated polychro...

Four strains of porcine parvovirus (PPV) were isolated from samples of organs of still-born sucklings and mummified aborted foetuses. The samples were isolated from the farms in Slovak Republic where the problems with reproduction and abortions in sows occurred. The isolated strains haemagglutinated guinea pig, murine, rat and chicken erythrocytes....

Asheg A. A., M. Levkut, V. Revajová, Z. ·evãíková, L. Kolodzieyski, J. Pistl: Dynamics of Lymphocyte Subpopulations in Immune Organs of Chickens Infected with Salmonella enteritidis. Acta Vet. Brno 2003, 72: 359-364. To determine the effect of different doses of Salmonella enteritidis on immunocompetent cells, the thymus, bursa of Fabricius, and pe...

The effect of application of omega-3 polyunsaturated fatty acids (omega-3 PUFA) on intestinal colonization by Lactobacillus paracasei and on cellular immunity has been investigated in gnotobiotic pigs. The administration of polyunsaturated fatty acids positively affected the adhesion of Lactobacillus paracasei to the jejunal mucosa of gnotobiotic p...

The effect of eight pesticides with different chemical structure (atrazine, bentazone, chloridazone, dichlofluanid, endosulfan, MCPA, simazine, triallate) on sheep peripheral blood phagocytes and lymphocytes was examined under in vitro conditions by iodo-nitro-tetrazolium reductase test and leukocyte migration-inhibition assay. The pesticides, diss...

Oil with n-3 polyunsaturated fatty acids (PUFA) was applied to suckling conventional and germ-free piglets. In an experimental group (EG) of piglets the level of growth factors was significantly higher ( P < 0.05), determined on the basis of somatomedin in the blood serum. Biochemical indices showed a significant increase in the level of γ-linoleni...

Adhesion and colonization of high (2 x 10(8) CFU) and low doses (2 x 10(2) CFU) of Salmonella enteritidis (phage type 4) was determined in the ceca collected 6 h-4 weeks after inoculation (pi), of 1-d-old White Plymouth Rock orally-inoculated chickens. S. enteritidis was associated with the epithelial surface of the villi in the low-dose group 18 h...

The immunotoxic effect of the herbicide chloridazone was evaluated on the basis of the metabolic activity of phagocytes and mitogenic activation of lymphocytes. In sheep exposed to a single acute dose of 80 mg/kg body weight (1/2 LD50) and metabolic activity of phagocytes, migration index of leukocytes and haematological parameters have been invest...

Blowfly larvae were used to accelerate the cleansing and healing process of chronic bedsores and suppurative ulcers in experimental rabbits. Phormia regina larvae were put directly into the treated wounds. A beneficial effect of a single application was observed after 3-6 days. Cleansing of wounds was selective and rapid. The animals tolerated the...

This work aimed to study the effect of organic-bound (metallic proteinate) trace elements -Zn, Mn, Cu, Fe, and Se - on live weight, concentrations of the elements in the blood serum, as well as phagocytosis, mitogen stimulation of lymphocytes, and the immunogenicity of inactivated oil vaccine against Aujeszky's disease. During the experiment, 15-we...

An 8-week experiment was carried out on piglets to observe the effects of organic trace element supplementation (Piglet Booster Alttech, Inc) on the micromineral profile of blood serum, immunological parameters, body weight (before and after weaning), and tissue concentrations of Zn, Fe, Cu, Mn, and Se. Forty piglets from 4 sows were divided into 2...

The effect of low and high doses of Salmonella enteritidis PT4 (SE) on immunocompetent cells in caecum and spleen of one-day-old chicks was investigated. Subsets of T lymphocytes positive for CD3, CD4, CD8 and B lymphocytes (Bu1b-positive cells) were counted in the caecum after immunohistochemical staining and the relative percentage of these cells...

Effect of pesticides with different chemical structure (atrazine, bentazone, chloridazone, dichlofluanid, endosulfan, MCPA, simazine, triallate) on sheep peripheral blood phagocytes was examined under in vitro conditions by iodo-nitro-tetrazolium reductase test (INT). The pesticides were dissolved in dimethylsulfoxide (the final concentration of pe...

Indices of cellular immunity in postpartum Holstein cows with the normal (n = 9) and prolonged (n = 4) uterine involution time were evaluated. Peripheral white blood cells were isolated by lysis from postpar- tum animals. An indirect immunofluorescence method for staining and flow cytometric analysis was employed to determine the cell subpopulation...

To study the immune response in poultry, a non-specific host for pheasant Eimeria colchici coccidium, ten-day-old chickens were each orally inoculated with 106 oocysts. For determination and comparison of coccidia invasion and development, histological examinations in poultry and pheasant chickens were made. In histological sections of four intesti...

The immunotoxic and genotoxic effect of endosulfan, an organochlorine insecticide, on sheep peripheral blood leukocytes was examined in in vitro conditions. The immunotoxic effect was evaluated by assays of the metabolic activity of phagocytes and assays for lymphocyte activation--the leucocyte migration-inhibition assay (LMIA) and lympho-prolifera...

Oral administration of oil with an increased content of omega-3 polyunsaturated fatty acids to germ-free piglets resulted in a significant increase in the total values of CD4, CD8 lymphocytes, B lymphocytes, and monocytes, in peripheral blood in comparison with the controls. The metabolic activities of phagocytes as well as the polyclonal activatio...

Chicks (1-d-old, three groups, each containing 50 chicks) were inoculated with 2 x 10(2) and 2 x 10(8) CFU of Salmonella enteritidis; the third group were kept as uninoculated control. Five birds from each group were euthanized at intervals from 6 h to 4 weeks post-inoculation (pi). In the low-dose group S. enteritidis was isolated from 60% cecal s...

The local immune response of chickens, as a non-specific host to, experimental infection with Eimeria colchici, coccidia from pheasants, was studied. The pheasant Eimeria invaded the intestine of the chickens not only in the caecum, as is the case in the natural host, but also in the cranial part of the intestine (duodenum). In these two infected a...

We examined time course, pathogenesis, and immune response of the developmental stages of Eimeria colchici in a specific host (pheasant chicks) and a non-specific host (Leghorn chicks). The number of E. colchici schizonts in the Leghorn chick caeca (1.4 ± 1.2) was significantly lower (P<0.005) than those in the pheasant chicks caeca infected with E...

The chromosomal aberration assay is the most common and sensitive method for detecting the biological damage induced by a great variety of mutagenic, carcinogenic and environmental agents. In this study an organochlorine insecticide, endosulfan, was investigated for its ability to induce chromosomal aberrations in vitro using sheep peripheral lymph...

DNA lesion induced by xenobiotics is implemented either through direct interaction of the chemical with DNA through covalent binding or intercalation, or indirectly, through interference with the processes of DNA replication and repair, interaction with proteins, nuclease release from lysosomes etc. The present study emphasizes that the assessment...

We examined the time course and histological localisation of the developmental stages of Eimeria colchici. The prepatent period in the caeca of pheasants was 6 days. The patent period began on day 7 post-infection (p.i.) and ended on day 11 p.i. with peak production of oocysts on days 8-9. The peripheral blood lymphocytes of pheasant chicks showed...

Flow cytometry was used to evaluate CD2, CD4, and CDS lymphocyte subpopulations in the peripheral blood of gnotobiotic piglets after administration of lactobacilli and followed E. coli K88+. Fourteen 1-day-old white Slovak crossbreed piglets were divided into four groups. Two experimental groups were given per os from days 2 to 4 Lactobacillus-the...

The organochlorine insecticide, endosulfan, was investigated for its ability to induce micronuclei in vitro in sheep peripheral lymphocytes. Lymphocytes from two donors were exposed to four different concentrations of insecticide (4 × 10-3, 4 × 10-4, 4 × 10-5 and 4 × 10-7 M) for 48 h. The lowest concentrations used (4 × 10-5 and 4 × 10-7 M) did not...

Flow cytometry was used to evaluate CD2, CD4, and CD8 lymphocyte subpopulations in the peripheral blood of gnotobiotic piglets after administration of lactobacilli and followed E. coli K88+. Fourteen 1-day-old white Slovak crossbreed piglets were divided into four groups. Two experimental groups were given per os from days 2 to 4 Lactobacillus - th...

Six cows suffering from mastitis were injected intramuscularly with tylosin at a dose of 10 mg kg-1 b.w. for five consecutive days after the morning milking. The milk samples were collected for 13 days at 12 h intervals, and from the afternoon and morning milkings were pooled together (1:1). The samples were analysed by liquid chromatography and yo...

In the present study we investigated the dynamics of selected parameters of cellular immunity (metabolic activity of phagocytes, bactericidal activity of phagocytes and polyclonal activation of lymphocytes) in improved Vallachian sheep during natural chronic intoxication with copper emitted by a copper producing industrial plant. Sheep were reared...

Oral administration of n-3 polyunsaturated fatty acids (PUFA) to piglets slightly enhances the immune response. As compared to the control, in the experimental piglets the absolute values of monocytes in the peripheral blood were significantly increased (P < 0.05), while the metabolic activity of phagocytes and the number of lymphocytes within the...

Kaãmár P., J. Pistl, I. Mikula: Immunotoxicology and Veterinary Medicine. Acta Vet. Brno, 1999, 68: 57-79. Immunotoxicology investigates unwanted adverse effects of xenobiotics (pesticides, heavy metals from emissions, new DNA-recombinant products, immune response modulators, monoclonal antibodies, drugs and others) on the immune system of man and...

Toxic and immunotoxic effects of p-chloroaniline--a metabolite of herbicide monolinuron--were investigated in peripheral blood leucocytic suspensions of five sheep using a migration-inhibition test. The toxic effect of p-chloroaniline was recorded at concentrations 1.0 to 0.1 mg.ml-1 and the immunotoxic one at concentrations 0.01-0.001 mg.l-1. The...

The effect of oral dosing with emissions containing heavy metals (mercury, lead, copper, cadmium, zinc and chromium) on immune status was tested in 3 groups of sheep. Two groups (Groups I and II) were given emissions for 15 d. One of these groups (Group I) was then also infested with Fasciola hepatica metacercaria on day 16. These 2 groups (Groups...

The objective of this investigation was to determine the effect of vitamin E administration on leucocyte parameters and some of their functions in transported calves. In the study 8 calves, aged approx. 10 days, divided into two groups, were used. 20 mg of tocopheryl-acetate per kg body weight were administered orally to each of the 4 experimental...

Immunoprophylactic and immunotherapeutic effects of specific actinobacillus DLE (dialysable leukocyte extract) were studied in model pigs experimentally infected with Actinobacillus pleuropneumoniae. Specific DLE used for immunoprophylactic purposes was administered intramuscularly to pigs either in two doses in combination with one dose of actinob...

The objective of the study was to test the effectiveness of Zn, Se and Cu supplementation through reticulum-rumen pellets and its effects on the rate of cellular and humoral reactions in sheep. An experiment included 12 lambing ewes of the Merino breed at the age of two years. Zn-containing pellets were applied via rumen to three lambing ewes at th...

A study of the effect of the Czechoslovakian herbicide BENTAZON TP on cells of the sheep immune system was carried out over a 3-mo period. A temporary decline in the number of T-lymphocytes in the peripheral blood was seen after 6 w of daily feeding of the herbicide. Dose-dependent statistically significant changes in the leucocyte migration index...

Activity of Salmonella-specific dialyzable leukocyte extracts (DLE) prepared from mesenteric lymphatic nodes of calves and stabilized with bovine albumin was studied in this work. The effect of ambient temperature and storage period on the activity of DLE was evaluated. Testing for DLE activity by means of capillary leukocyte migration inhibition (...

The protective effect of dialyzable leukocyte extract (DLE) was investigated in the experimental model of Salmonella infection in calves. DLE was obtained from the lymphatic nodes and spleens of fattening bulls immunized with whole-cell Salmonella vaccine (designated DLEs-im), from the same organs of calves immunized and subsequently infected with...

Mikula I., J. PistI, P. Kacmar: Immune Response of Sheep at Subchronic Into­ xication by Pyrethroid Insecticide Supercypermethrine. Acta vet. Brno, 61, 1992: 57-60. Testing of the influence of subchronic (6 weeks) load of sheep organism caused by the pyrethroid insecticide supercypermethrine on functionality of cells of the immune system was carrie...