Jung sung Kim

• PhD.
• Professor at Chungbuk National University

Background The genus Chrysanthemum (Asteraceae) consists of a series of polyploids, ranging from diploid (2x) to decaploid (10x), with a basic chromosome number of x = 9. They vary widely within and among species owing to natural hybridization and polyploidization in natural habitats, and this has made recognizing the taxonomic boundaries among the...

The genus Vandenboschia exhibits significant diversity, driven by complex hybridization events and varying ploidy levels in the natural habitats of Korea, leads to misidentifications that obscure its true distribution and classification. To address this issue, the present study employed morphological, cytological, and genotype analyses to clarify t...

i>Chrysanthemum zawadskii Herbich is widely distributed from northern Europe to East Asia. It has been reported that various ploidy levels ranging from diploid (2 n = 2 x = 18) to decaploid (2 n = 10 x = 90) are distributed in Korea. The diploid populations of C. zawadskii , however, have limited habitats, with only four populations from two locati...

In this study, we determined the chloroplast genome sequence of the Austral king fern, Todea barbara (L.) Moore. The plastome of T. barbara is a typical circular form composed of 144,208 bp with two inverted repeats (IRs; 10,442 bp), a large single copy (LSC; 101,059 bp), and a small single copy (SSC; 22,265 bp). The complete sequence comprises 131...

Natural hybridization between Asplenium incisum and A. ruprechtii has been observed in Northeast Asia and its allotetraploid species, A. castaneoviride, was reported. However, the hybridization process between the parental species and the origin of the allotetraploid taxon remains obscure. Additionally, the systematic affinities of the recently des...

The complete chloroplast genome sequence of Crepidomanes latealatum (Bosch) Copel. was determined in the present study. The genome is 145,943 base pairs (bp) in length and comprised two inverted repeats (32,990 bp) between a large single copy (92,170 bp) and a small single copy (20,783 bp). It contains 88 coding genes, 8 rRNA genes, 34 tRNA genes,...

The complete chloroplast genome sequence of Acer pseudosieboldianum (Sapindaceae) was determined. The chloroplast genome of A. pseudosieboldianum is 157,053 bp in length with two inverted repeats (26,747 bp) between a large single-copy (85,391 bp) and a small single-copy (18,168 bp). The GC content was 37.8% and it was composed of 86 coding genes,...

COLE TCH, HILGER HH, SCHNEIDER H, KIM HT, KIM JS (2022) Pteridophytes 양치식물, 석송류와 고사리류 • Korean version of: COLE, HILGER, SCHNEIDER (2022) Pteridophytes Phylogeny Poster (PtPP) • 분자 계통 데이터를 기반으로 분석하여 가정한 계통수 • 계통수의 가지는 변형된 것으로 가지의 길이는 실제 시간 척도를 표현한 것이 아님 • 속/종수의 추정치는PPG 1 (Schuettpelz et al. 2016) 을 따름 © Cole, Hilger, Schneider, Kim, Kim 2022 (CC-BY...

A new allotetraploid species of the genus Asplenium, A. pseudocapillipes, originated from the hybridization between A. capillipes and A. tenuicaule, has been newly discovered in two limestone areas of South Korea. A molecular phylogenetic analysis using one chloroplast region (rbcL) and three single- or low-copy nuclear regions (AK1, gapCp, pgiC) a...

We have sequenced the Ziziphus jujuba cv. Bokjo chloroplast genome by de novo assembly using next-generation sequencing. The complete circular chloroplast genome consisted of 161,714 bp and contained four parts: a large single-copy (LSC) region of 89,323 bp, a small single-copy (SSC) region of 19,361 bp, and two inverted repeat regions (IRa and IRb...

Valeriana sambucifolia f. dageletiana (Nakai. ex Maekawa) Hara is a broad-leaved valerian endemic to Ulleung Island, a noted hot spot of endemism in Korea. However, despite its widespread pharmacological use, this plant remains comparatively understudied. Plant cells generally contain two types of organellar genomes (the plastome and the mitogenome...

The complete chloroplast genome sequence of Haplopteris flexuosa, a member of Vittarioideae (family Pteridaceae), was determined. The chloroplast genome of H. flexuosa was 165,664 bp in length with two inverted repeats (32,556 bp) between a large single copy (79,996 bp) and a small single copy (20,556 bp). The GC content was higher than that of rel...

Ferns have conspicuous sporophytes as the dominant phase in their life cycle; however, the gametophytes are completely separated from the sporophytes and supply their own nutrition, unlike in bryophytes and seed plants. Among the gametophytes, some maintain their populations in the gametophyte phase without progressing to sporophyte production and...

Fern gametophytes have often been neglected in research; however, studies on gametophytes are crucial for a better understanding of the evolution of ferns. During their life cycle, some gametophytes produce large and long‐lived populations without producing sporophytes and reproduce independently through asexual means, such as through the formation...

In this study, four plastomes of Hymenophyllum, distributed in the Korean peninsula, were newly sequenced and phylogenomic analysis was conducted to reveal (1) the evolutionary history of plastomes of early-diverging fern species at the species level, (2) the importance of mobile open reading frames in the genus, and (3) plastome sequence divergenc...

The complete chloroplast (cp) genome sequence of LHeucanthemella linearis was newly analyzed in this study. It was 151,395 bp in length and was a typical circular structure composed of a large single-copy region (LSC) (83,080 bp) and a small single-copy (SSC) region (18,391 bp) which were separated by two inverted repeat regions (24,962 bp). It was...

The complete chloroplast (cp) genome sequence of Ajania pacifica, called as golden and silver chrysanthemum, was newly analyzed in this study. It was 151,059 bp in length and was a typical circular structure composed of and comprised of a large single copy region (82,857 bp) and a small single copy region (18,294 bp) which were separated by two inv...

The complete chloroplast (cp) genome sequence of Thymus quinquecostatus var. japonicus, an endemic species to Ulleung Island of Korea and used as plant material for folk remedies, was firstly analyzed in this study. It showed a typical circular structure composed of 151,782 bp in length and comprised of a large single-copy region (82,903bp) and a s...

It is very important to confirm and understand the genetic background of cultivated plants used in multiple applications. The genetic background is the history of crossing between maternal and paternal plants to generate a cultivated plant. If the plant in question was generated from a simple origin and not complicated crossing, we can easily confi...

The genus Lilium L. is widely distributed in the cold and temperate regions of the Northern Hemisphere and is one of the most valuable plant groups in the world. Regarding the classification of the genus Lilium, Comber’s sectional classification, based on the natural characteristics, has been primarily used to recognize species and circumscribe the...

The complete chloroplast (cp) genome sequence of Acer takesimense, a member of maple tree and an endemic species to Ulleung Island of Korea, was firstly analyzed in this study. It was formed a typical circular structure of 157,023 bp in length and composed of a large single copy region (85,371 bp) and a small single copy region (18,160 bp) which we...

The characteristic of complete chloroplast (cp) genome sequence of Crepidiastrum lanceolatum, a member of the subfamily Cichorioideae with unresolved phylogenetic relationship among the related taxa, was firstly reported in this study. It was 152,748 bp and contained a large-single copy region (SSC) (84,022 bp) and a small-single copy region (LSC)...

Liliaceae sensu APG IV include c. 600 species, and have been circumscribed into three subfamilies, Lilioideae, Calochortoideae and Streptopoideae. Molecular phylogenetic approaches to this family have produced dynamic changes in the generic circumscriptions. We conducted molecular phylogenetic analyses, time estimations and biogeographical analyses...

Interspecific and intergeneric relationships of Prunus s.l. are still unclear due to low levels of genetic variation among species, and resulting partially unresolved phylogenetic inferences. Here we sequenced and compared six complete plastomes from two subgenera of Prunus in order to choose molecular markers to increase the amount of genetic vari...

The characteristic of complete chloroplast (cp) genome sequence of Chrysanthemum lucidum, one of famous insular plant and an endemic to Ulleung Island of Korea, was firstly introduced in the present study. It was 150,985 bp and contained a large single copy region (82,786 bp) and a small single copy region (18,281 bp) which were separated by two in...

The complete chloroplast (cp) genome sequence of Rheum wittrockii, an endangered and medicinal plant of Kazakhstan, was firstly determined in the present study. It was 159,051 bp and contained a large single copy region (84,750 bp) and a small single copy region (12,999 bp) which were separated by two inverted repeat regions (30,651 bp). In total,...

Hydrangea luteovenosa is a member of the family Hydrangeaceae. The complete chloroplast genome sequence of H. luteovenosa was characterized from MiSeq (Illumina Co.) pair-end sequencing data. The chloroplast genome of H. luteovenosa was 157,494 bp in length with a pair of inverted repeats (IRs) (25,126 bp) which were separated by a large single-cop...

Melanthiaceae (Liliales) comprise 17 genera of rhizomatous or bulbous perennials and are distributed across the Northern Hemisphere. The relationships among the five tribes in this family have been evaluated in many molecular and morphological studies. In this study, we performed a phylogenetic analysis of the 17 genera, including 106 species of Me...

Genomic events including gene loss, duplication, pseudogenization and rearrangement in plant genomes are valuable sources for exploring and understanding the process of evolution in angiosperms. The family Melanthiaceae is distributed in temperate regions of the Northern Hemisphere and divided into five tribes (Heloniadeae, Chionographideae, Xeroph...

Earlier research has revealed that the ndh loci have been pseudogenized, truncated, or deleted from most orchid plastomes sequenced to date, including in all available plastomes of the two most species-rich subfamilies, Orchidoideae and Epidendroideae. This study sought to resolve deeper-level phylogenetic relationships among major orchid groups an...

The phylogenetic positions of the families Campynemataceae and Corsiaceae within the order Liliales remains unclear. To date, molecular data from the plastid genome of Corsiaceae has been obtained exclusively from Arachnitis, for which alignment and phylogenetic inference has proved difficult. The extent of gene conservation among mycoheterotrophic...

Reliable and accurate species identification is essential to establish a strategy for monitoring and manipulation of plant populations for conservation biology. However, identification of nonflowering plants is difficult. Based on recent advances in molecular biological techniques, various molecular identification methodologies have been proposed a...

Main conclusion: The complete chloroplast genome of two colchicine medicinal plants is reported for the first time. Deletion of ycf 15 gene occurred only in Colchicum but not in Gloriosa and suggests this as a potential marker for delineating the two species. Colchicum autumnale L. and Gloriosa superba L. are well-known sources of colchicine, a ty...

Plastid genome studies on Orchidaceae, one of the largest families of flowering plants, have been limited to the subfamily Epidendroideae. To increase understanding of the plastid genome evolution in Orchidaceae, we performed a complete plastid genome analysis of the slipper orchid Cypripedium japonicum positioned in the basal clade of the family....

Abstract Bomarea, a member of the family Alstroemeriaceae, is distributed from Chile to Mexico and includes approximately 120 species. Recent molecular phylogenetic studies have clarified the monophyly of the family within the order Liliales and the sister relationship with the family Colchicaceae. At this time, five plastid genomes of Liliales hav...

Abstract The complete chloroplast (cp) genome sequence of Eustrephus latifolius was firstly determined in subfamily Lomandriodeae of family Asparagaceae. It was 159,736 bp and contained a large single copy region (82,403 bp) and a small single copy region (13,607 bp) which were separated by two inverted repeat regions (31,863 bp). In total, 132 gen...

The chloroplast is an essential plant organelle responsible for photosynthesis. Gene duplication, relocation, and loss in the chloroplast genome (cpDNA) are useful for exploring the evolution and phylogeny of plant species. In this study, the complete chloroplast genome of Paris verticillata was sequenced using the 454 sequencing system and Sanger...

The complete chloroplast genome of Chionographis japonica (Willd.) Maxim. (Melanthiaceae, Liliales) was mapped using polymerase chain reaction and the Sanger method. The circular double-stranded DNA was a typical quadripartite structure consisting of two inverted repeated regions (27,397 bp), a small single copy region (18,205 bp), and a large sing...

Primers used for the sequence mapping of C. japonica, separated by region (LSC, SSC, IR) and named according to the appropriate location within the Chionographis sequence. Primers compared with S. china and those with greater than 80 % similarity are listed along with nucleotide position compared with Chionographis

Colchicaceae (Liliales) comprise c. 250 species and 15–21 genera of rhizomatous or cormous perennials and are distributed through temperate and tropical Africa, Europe, Asia and North America. They have been traditionally divided into two subfamilies, but this division is still unclear and the phylogenetic relationships among members of the family...

Aligned matrix of combined 79 genes for the present study. (DOCX)

Substitution rates and sequence variations among 3 families of Liliales. (DOCX)

Electrophoretogram of isolated plastid DNA in the present study. M) lambda-HindIII digest, Lil) Liliumlongiflorum (268 ng/µl), Als) Alstroemeriaaurea (314 ng/µl). (TIF)

Substitution models for each gene used in the phylogenetic study. (DOCX)

Comparison of the sequence variation between two Lilium species. (DOCX)

Monocots are one of the most diverse, successful and economically important clades of angiosperms. We attempt to analyse the complete plastid genome sequences of two lilies and their lengths were 152,793bp in Liliumlongiflorum (Liliaceae) and 155,510bp in Alstroemeriaaurea (Alstroemeriaceae). Phylogenetic analyses were performed for 28 taxa includi...

Many molecular studies have shown the monocot order Liliales to be well supported; morphologically, it is defined by synapomorphies of tepalar nectaries and extrorse anthers, in contrast with septal nectaries and introrse anthers commonly found in other monocots, especially Asparagales, with which it was often confused in the past. It comprises c....

We performed a phylogenetic analysis of 13 families of Asparagales sensu APG III, including 59 Korean taxa representing 10 families based on five plastid regions sequences (matK, rbcL, rpoC1, rps3, and atpF-H intergenic spacer). Parsimony and Bayesian analyses were conducted to clarify the relationships among Korean Asparagales. Among the five regi...

Schisandra chinensis (Turcz.) Baill. is a climbing woody vine found over a wide geographical range in northeast Asia. Its red five-flavored fruits have been used as drugs and food. In China and Korea, the dried fruit of S. chinensis has been used for a long time as a traditional medicine. Three species of the family Schisandraceae, S. chinensis, S....

DNA barcoding involves sequencing a standard region of DNA as a tool for species identification. However, there has been no agreement on which region(s) should be used for barcoding land plants. To provide a community recommendation on a standard plant barcode, we have compared the performance of 7 leading candidate plastid DNA regions (atpF–atpH s...

Polyploidy, which is thought to have played an important role in plant evolution and speciation, is prevalent in Chrysanthemum (x = 9). In fact, polyploid series are known in C. zawadskii (2x, 4x, 6x, 8x, and 10x) and C. indicum (2x, 4x, and 6x), but the mechanism by which polyploidization occurs is unknown. Here we show that in diploid individuals...

DNA barcoding involves sequencing a standard region of DNA as a tool for species identification. However, there has been no agreement on which region(s) should be used for barcoding land plants. To provide a community recommendation on a standard plant barcode, we have compared the performance of 7 leading candidate plastid DNA regions (atpF–atpH s...

Diploid individuals (2n=18) of Chrysanthemum zawadskii, C. indicum and C. boreale, two or all three of which often occur together in the wild in East Asia, have diverse metaphase karyotypes. We hypothesised that the diverse karyotypes result from chromosome rearrangements. To test this, we investigated chromosomal behaviour during pollen mother cel...

Although the Dendranthema zawadskii complex has been known to comprise a series of polyploids (4x, 6x, 8x), we found diploid individuals (with 2 n=18) to occur in four populations of D. zawadskii var. latilobum in the southern region of Korea. Karyotypes of metaphase chromosomes were diverse because numbers of metacentric, submetacentric, and acroc...

This is a report of a natural hybrid between Pseudostellaria davidii and Pseudostellaria palibiniana. Mulitivariate analysis with 19 morphological characters demonstrated that an unidentified taxon was intermediate between P. davidii and P. palibiniana. Somatic chromosome numbers of these three taxa were all identical (2n = 32). Pollen sterility wa...