Junbo Xie

Junbo Xie
Tianjin University of Traditional Chinese Medicine · College of Traditional Chinese Pharmacy

Doctor of Pharmacy

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65
Publications
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919
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Publications

Publications (65)
Article
Neurological disorders generally have the characteristics of occult onset and progressive development, which can do some serious damage to human body in the aging process. It is worth noting that traditional medicine can potentially prevent and treat such diseases. Zizyphi Spinosae Semen (ZSS), the seeds of Ziziphus jujuba var. spinosa it has a lon...
Article
Platycodonis Radix (Jiegeng), the dried root of Platycodon grandiflorum, is a traditional herb used as both medicine and food. Its clinical application for the treatment of cough, phlegm, sore throat, pulmonary and respiratory diseases has been thousands of years in China. Platycodin D is the main active ingredient in Platycodonis Radix, which belo...
Article
The effect of flavonoid glucosides from seeds of Ziziphus jujuba var. spinosa(Bunge) Hu ex H.F. Chow, Rhamnaceae, on learning and memory was investigated in mice with scopolamine-induced dysmnesia. In Y-maze and passive avoidance test, the results showed that the flavonoid-rich extract could decrease the number of errors and increase the latency ti...
Article
In this work, a novel GABABR1a receptor biosensor was developed for the first time. The GABABR1a protein was obtained by liposome transfection with HEK‐293T cells. The GABA, jujuboside A and baclofen were detected by time‐current method with the biosensor. The results showed that the action regularity of GABA, jujuboside A and baclofen on GABABR1a...
Article
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This work aims to investigate antioxidant activities of saponins extracts from Zizyphus jujuba Mill var. spinosa leaves (ZSL). In this study, saponins were extracted by ultrasound-assisted method, Box-Behnken design (BBD) and response surface methodology (RSM) were applied to obtain the optimal extraction conditions were as follows: ratio of liquid...
Article
The transient receptor potential vanilloid 1 (TRPV1) is a key target for the spicy taste sensor and analgesic drug development. However, the human TRPV1-associated signaling remains to be obscure. In this study, we overexpressed human TRPV1 (hTRPV1) in HEK293T cells and explored its signaling activated by spicy substances. A cell membrane biosensor...
Article
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6ʹʺ‐p‐coumaroylspinosin (P‐CS) is a flavonoid isolated from Ziziphi Spinosae Semen (ZSS), whereas, the antioxidative activity has not been reported. Oxidative stress is believed to be one of the main causes of neurodegenerative disorders. In this study, the antioxidative effect of P‐CS on PC12 cells was determined. The cells were treated with acryl...
Article
In this study, the effect of Ziziphus jujuba Mill var. spinosa seeds (ZSS) polysaccharides on the bioavailability of spinosin in mice and its molecular mechanism were investigated. After continuously fed with ZSS polysaccharides 100 mg/kg·d⁻¹ for 28 consecutive days, the C57BL/6 mice absorbed spinosin at an obvious lower level compared with the con...
Article
Background Flavonoid compounds are one kind active ingredients isolated from a traditional Chinese herb Zizyphi Spinosae Semen (ZSS). Studies have shown that ZSS flavonoids have significant antioxidant effects. Methods In this study, Caco-2 cell monolayer model was constructed to investigate the intestinal absorption characteristics and mechanism...
Article
Although the bioactive compounds in goji have been thoroughly identified and quantified, little information is available on the bitter compounds in the berries, and no systematic works on the substances responsible for their bitterness have been performed. Herein, the substances contributing to the bitterness of berries were isolated and purified f...
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This study aims to investigate the neuroprotective effects of 6‴-feruloylspinosin (6-FS), one of the main active flavonoid components in Sour Jujube seeds, on beta-amyloid (Aβ) protein transgenic Caenorhabditis elegans (GMC101) and PC12 cells, and determine the molecular mechanism of its action. We found that 6-FS could ameliorate the progression o...
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In this study, an efficient HPLC method was developed and validated to determine seven polyphenols, i.e. chlorogenic acid, caffeic acid, protocatechuic acid, geniposide, rutin, quercetin and isorhamnetin, in different parts (leaves, barks and fruits) of Eucommia ulmoides. A Luna C18 column (150 mm × 3.9 mm, 5.0 μm) was applied and eluted with a gra...
Article
In the present study, a glucogalactomanan polysaccharide isolated from Agaricus bisporus named as TJ3 was investigated its immunomodulatory effects and molecular mechanisms in RAW 264.7 cells. Functional analysis showed TJ3 could inhibit the proliferation of RAW 264.7 cells in a certain concentration range. Moreover, TJ3 treatment increased the exp...
Article
Ethnopharmacological relevance: Sour Jujube seed from Ziziphus jujuba Mill. var. Spinosa (Bunge) Hu ex H. F. Chow is a traditional Chinese herb. It was demonstrated with significant activities in anti-depression and antioxidant by numerous pharmacological studies. Flavonoids is one of the main constituents in sour Jujube seed. Aim of the study:...
Article
Objectives The aim of this research was to investigate the intestinal absorption characteristics and mechanisms of spinosin (SPI), and a new dosage form was prepared to increase the intestinal absorption of SPI. Methods In this study, the intestinal absorption characteristics and mechanisms of SPI were first investigated using in situ absorption m...
Article
In this study, the polysaccharide was extracted from Ziziphus jujuba Mill var. spinosa seeds (ZSS) with ultrasound assisted extraction method (UAE). Box-Behnken design (BBD) and response surface methodology (RSM) were applied to obtain the optimal conditions for perfect yield of ZSS polysaccharide: extraction temperature 52.5 °C, extraction time 21...
Article
Full-text available
Zizyphi Spinosae Semen (ZSS) is an herbal medicine with a variety of biological activities. In this study, the oil fraction of ZSS was extracted using an ultrasonic assisted extraction (UAE) method and the value of this underutilized byproduct was explored. The effects of operational parameters of the UAE process (extraction temperature, extraction...
Article
In this study, a taste bud tissue biosensor was prepared by a starch-sodium alginate cross-linking fixation method. Capsaicin was used as a TRPV1 noxious ion channel activator to investigate the antagonism kinetics of six different substances on capsaicin. The results showed that capsazepine, AMG517, loureirin B, and tetrahydropalmatine were all co...
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A simple high performance liquid chromatography (HPLC) method was established for identification and quantification of nine polyphenols (gallic acid, chlorogenic acid, caffeic acid, myricetrin, hyperoside, luteoloside, quercitrin-3-rhamnoside, quercetin and kaempferol) in Polygoni Avicularis Herba. The analysis was performed on a Pinnacle II C18 co...
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Agaricus bisporus is one of the most important edible and medicinal mushrooms in the world. It has been well known that Agaricus bisporus has an immunoregulatory role, but its active ingredients have not been completely identified. In this study, a glucogalactomannan named as TJ3, was isolated and purified from Agaricus bisporus. TJ3 (827 KDa) is c...
Article
Ganoderma lucidum is one of the most commonly used mushrooms in traditional Chinese medicine, with significant immunomodulatory and antitumor effects. Triterpenoids are deemed to be the main bioactive components in G. lucidum. In this study, high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (MS) and high-performance...
Article
Jujuboside A (JuA), an active saponin, is responsible for the anxiolytic and sedative effects of Zizyphi Spinosae Semen (ZSS). In this study, the gastrointestinal absorption and metabolic dynamics of JuA were investigated in vivo and in vitro. The results showed that the bioavailability was 1.32% in rats, indicating only a trace amount of JuA was a...
Article
In this study, a novel galactomannoglucan named as TJ2 was isolated from Agaricus brasiliensis with microwave extraction, macroporous resin, ion exchange resin and high resolution gel chromatography. TJ2 is composed of glucose, mannose and galactose in the ratio 99.2:0.2:0.6. Infrared spectra (IR), methylation analysis and nuclear magnetic resonanc...
Article
6'''-p-coumaroylspinosin (P-CS), a bioactive flavonoid, is typically extracted from Semen Ziziphi Spinosae (SZS). In this study, a HPLC-MS/MS method was developed to determine P-CS for investigating the degradation characteristics of P-CS incubated with rat feces. The results showed that P-CS degraded rapidly and the degradation speeds varied depen...
Article
Full-text available
A novel and green electrochemical immunosensor for detection of Staphylococcal enterotoxin Q (SEQ), a toxic superantigen that can induce severe food poisoning and even fatal conditions, was developed by fixing double-layer gold nanoparticles (AuNPs), horseradish peroxidase, and thionin-chitosan composite membrane on the glassy carbon electrode surf...
Article
Full-text available
In the current study, a novel double-layer gold nanoparticles- electrochemical immunosensor electrode (DGN-EIE) immobilized with Salmonella plasmid virulence C (SpvC) antibody was developed. To increase the fixed quantity of antibodies and electrochemical signal, an electrochemical biosensing signal amplification system was utilized with gold nanop...
Article
At present, developing an efficient assay method for truly reflecting the real feelings of gustatory tissues is of great importance. In this study, a novel biosensor was fabricated to investigate the kinetic characteristics of the receptors in taste bud tissues sensing bitter substances for the first time. Porcine taste bud tissues were used as the...
Article
Zizyphi Spinosi Semen (ZSS) has a long history of sedative-hypnotic use in China. As a novel flavone C-glycoside, coumaroylspinosin is a main flavonoid only found in ZSS. Up to now, its pharmacokinetic information and tissue distribution in vivo are not available yet. With a simple, rapid and sensitive HPLC–MS/MS method, the pharmacokinetics and ti...
Article
A sensitive and reliable high-performance liquid chromatography with tandem mass spectrometry was developed and validated for the quantification of swertisin in rat plasma. The samples were prepared with methanol by protein precipitation. Swertisin was separated by using an Agilent Poroshell 120 EC-C18 column (4.6 mm × 50 mm, 2.7 μm) with the mobil...
Article
6‴-Feruloylspinosin, a C-glycoside flavonoid, is the active ingredient responsible for the anxiolytic and sedative effects of Zizyphi Spinosiae Semen. However, the degradation mechanism of 6‴-feruloylspinosin in vivo is largely unknown. In this study, we developed a simple and rapid method for determining the degradation of 6‴-feruloylspinosinin in...
Article
A novel non-enzymatic glucose sensor based on a glassy carbon electrode modified with Pt3Ru1 alloy nanoparticles (Pt3Ru1/GCE) was fabricated. Pt3Ru1 alloy nanoparticles were prepared by a reverse microemulsion method at room temperature. The X-ray diffraction (XRD) and energy dispersive X-ray spectroscopy (EDS) demonstrate that Pt3Ru1 nanoparticles...
Article
A sensitive, reliable and accurate HPLC-MS/MS method was developed and validated for the quantification of 6”'-feruloylspinosin in rat plasma and tissues with puerarin as the internal standard. The separation was performed on a Proshell 120 EC-C18 column (4.6 × 150 mm, 2.7 μm) with a mobile phase consisting of acetonitrile and 0.1% formic acid (20:...
Article
The royal sun mushroom, Agaricus brasiliensis is a widely consumed mushroom around the world. In this study, the immunoregulatory potential of A. brasiliensis polysaccharides was investigated in vitro and in vivo. In vivo, the polysaccharides remarkably increased the spleen and thymus indexes in mice, and this effect was influenced significantly by...
Article
Full-text available
Prostate cancer is the second most commonly diagnosed cancer among men in the United States. Prostate cancer therapy is severely hampered by lack of response and development of resistance to conventional chemotherapeutic drugs in patients. Therefore, the development and discovery of new drugs have become an urgent clinical need. Interferons (IFNs),...
Article
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FcRn (neonatal Fc receptor) plays an important role in IgG transportation, antigen presentation and signal transmission. In this study, the complement fixation test and flow cytometry test were performed to verify whether the heterologous antibody could be transmitted to the serum or leukocyte with FcγR (Fc gamma receptor) across the intestinal muc...
Article
A new HPLC–MS/MS method was developed and validated for simultaneous determination of stachydrine and leonurine (two main bioactive alkaloids) in Herba Leonuri and its succedaneum—Herba Lagopsis. Chromatographic separation was performed on an Agilent Eclipse Plus C18 (100 mm × 2.1 mm, 3.5 µm) and methanol–0.1% formic acid solution (20:80) was used...
Article
A sensitive and credible high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method was established and validated for the determination of isovitexin in rat plasma and various tissues (including heart, liver, lung, kidney, stomach, intestine, muscle, brain and cerebellum). The samples were prepared with methanol by liquid-l...
Article
Jujuboside A (JuA), a dammarane-type triterpene glycoside, is a main bioactive saponin in Zizyphi Spinosi Semen (a traditional Chinese herbal food). In this research, the distribution of JuA in Sprague-Dawley rats was investigated with a new and efficient HPLC–ESI-MS/MS method. The results showed that JuA distributed rapidly and widely in various r...
Article
A novel taste biosensor based on ligand-receptor interaction was developed through fixing taste-bud tissues of SD rats to a glassy carbon electrode. Using the sodium alginate-starch gel as a fixing agent, taste-bud tissues of SD rats were fixed between two nuclear microporous membranes to make a sandwich-type sensing membrane. With the taste biosen...
Article
Full-text available
The energy substances (mainly carbohydrates and fats) are the basis and guarantee of life activity, especially the oxidative phosphorylation for energy supply. However, excessive absorption and accumulation of these substances can lead to metabolic diseases such as obesity, hyperlipidemia, diabetes, and cancers. A large amount of studies demonstrat...
Article
The transport of heterogeneous IgG to Fc receptor-expressing leukocytes and serum was investigated by the complement fixation test in mice. The results showed that heterogeneous IgG could be transported into the blood by neonatal Fc receptor (FcRn) on the gut mucosal surface. The interactions between human FcRn and IgG of different animal species w...
Article
Jujuboside A (JuA) is a main active ingredient of semen ziziphi spinosae, which can significantly reduce spontaneous activity in mammals, increase the speed of falling asleep, prolong the sleeping time as well as improve the sleeping efficiency In this study, the mechanism and the pathway of the sedative and hypnotic effect of JuA were investigated...
Article
Full-text available
Ziziphi Spinosae Semen (ZSS), the seed of Ziziphus jujuba var. spinosa (Bunge) Hu ex H. F. Chow., is a traditional herb for insomnia and anxiety in eastern Asia. However, few researches have been concerned with its effect on ameliorating memory and learning performance. To investigate the constituents of ZSS water soluble extract and its ameliorati...
Article
Jujuboside A (JuA) is a representative saponin with significant sedative and hypnotic effects. Up to now, there were many arguments on its metabolic mechanism. In this study, a high performance liquid chromatography-tandem mass spectrometry (MS/MS) method was developed for investigating the degradation characteristics of JuA incubated with rat fece...
Article
A new liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated for simultaneous determination of glycyrrhizin, formononetin, glycyrrhetinic acid, liquiritin, isoliquiritigenin, and licochalcone A in licorice. An Eclipse Plus C-18 column (I.D. 4.6 x 100 mm, 3.5 mu m particle size; Agilent) was used in the analysis...
Article
Spinosin, a flavone-C-glycoside, is a bioactive ingredient isolated from a traditional Chinese herb Zizyphi Spinosi Semen. In this study, a new high-performance liquid chromatography–electrospray ionization–mass/mass spectrometry method was developed and validated to determine spinosin in brain tissues including olfactory region, hippocampus, corpus...
Article
Full-text available
Agaricus bisporus is one of the most consumed culinary mushrooms in the world. The white type of Agaricus bisporus has been demonstrated with various bioactivities beneficial to human health. However, little was known about its brown type. In this study, the anti-cancer and immunoregulatory potential of Agaricus bisporus (brown) polysaccharide were...
Article
Jujuboside B (JuB) is a main bioactive saponin constituent of Ziziphi Spinosae Semen, which is a traditional herb for the treatment of insomnia and anxiety. However, the detailed metabolic mechanism of JuB is poorly understood. In this study, a novel method of rapid resolution liquid chromatography-triple quadrupole mass spectrometry was developed...
Article
A rapid, sensitive, and selective liquid chromatography–tandem mass spectrometric (HPLC-MS/MS) method was developed and validated for the determination of spinosin, a pharmacologically active constituent isolated from Zizyphi Spinosi Semen (ZSS). This method was used to examine the degradation kinetics of spinosin by rat intestinal flora and to elu...
Article
Compound Danshen injection (CDSI, a traditional medicine) is an effective drug for the treatment of cardiovascular and cerebrovascular diseases. However, the research about its stability is absent. A new high-performance liquid chromatography method was developed to assay its main effective constituents, i.e., propanoid acid (PA), protocatechuic al...
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Full-text available
How do functional foods affect human health? To answer this question it is important to understand what happens when food is digested and taken up by the gastrointestinal (GI) tract. The gut is a selective nutrient absorption system and the most important signal transduction and information exchange system within the body. It acts as a signal trans...
Article
An HPLC method coupled with triple quadrupole mass spectrometry (MS/MS) was applied to the determination of tanshinone IIA (TanllA), salvianolic acid B (SA-B), protocatechuic acid (PA), protocatechuic aldehyde (PAH), paeoniflorin (PF), and ferulic acid (FA) in Guanxinll prescription. These six compounds were analyzed simultaneously with YMC ODS-AQ...
Article
A new and rapid method was developed for simultaneous identification and determination of 11 polyphenols in Herba lycopi, i.e. rosmarinic acid, protocatechuic aldehyde, protocatechuic acid, caffeic acid, ferulic acid, apigenin, luteolin, quercetin, isorhamnetin, chlorogenic acid and rutin, using high performance liquid chromatography (HPLC) coupled...
Article
In this study, the composition of fatty oil from Semen Ziziphi Spinosae and its cardiotonic activity on the heart isolated from a toad were studied. Oil-in-water (O/W) emulsions of fatty oil were prepared by the perfusion method. The fatty oil had a positive inotropic effect on isolated rat hearts at a concentration between 5 × 10(-3) and 2 × 10(-2...
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This study aims to explore and evaluate the effects of Semen Ziziphi Spinosae extracts on the serum levels of interleukin (IL)-6, IFN-γ, IL-1β, TNF-α, IL-10 and IL-4 in mice, and the regulative effect of Semen Ziziphi Spinosae on the cytokine system. Using an ELISA assay, the serum levels of IL-6, IFN-γ, IL-1β, TNF-α, IL-10 and IL-4 were examined i...
Article
A new and rapid method was developed for simultaneous determination of mangiferin, neomangiferin, timosaponin A-III, and C in Rhizoma Anemarrhenae using rapid resolution liquid chromatography coupled with triple quadrupole mass spectrometry. The analysis was performed on an Eclipse Plus C(18) column (I. D. 4.6 x 100 mm, 3.5 mu m). Electrospray ioni...
Article
Various methods have been published for the analysis of these compounds in licorice, including high-performance liquid chromatography (HPLC) [14–17], high-performance thin-layer chromatography (HPTLC) [18], and capillary electrophoresis [19, 20]. However, these published methods mainly involve Glycyrrhiza glabra and G. uralensis [21–22]. There are...
Article
A sensitive HPLC method with simple extraction was developed for simultaneous determination of huperzine A (HupA) and huperzine B (HupB) in Huperzia serrata, H. crispata, H. miyoshiana, and Lycopodiastrum casuarinoides. In order to avoid conventional multiple-step and time-consuming sample preparation methods, direct reflux extraction with alkaline...
Article
A reverse phase high performance liquid chromatographic (HPLC) method with evaporative light scattering detection (ELSD) was developed for simultaneous determination of jujuboside A, B and betulinic acid in semen Ziziphi spinosae. The analysis was performed by gradient elution, using an aqueous mobile phase (containing 0.1% acetic acid) modified by...
Article
A high performance liquid chromatography coupled with photodiode-array detection method was developed for simultaneous determination of glycyrrhizin, paeoniflorin, benzoic acid, quercetin, ferulic acid, formononetin, liquiritin and isoliquiritigenin in the Chinese proprietary medicine "Xiao Yao Wan" (XYW). The analysis was performed by reverse phas...

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