Jun Zhang

Jun Zhang
Texas Tech University Health Sciences Center | TTUHSC · Department of Biomedical Sciences

Ph.D., Sc.D.

About

116
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Publications

Publications (116)
Article
Full-text available
Current understanding of phosphotyrosine binding (PTB) domain is limited. Recently, we revealed a novel atypical phosphotyrosine binding (aPTB) domain in CCM2, making it a dual PTB domain-containing protein. Since aPTB domain is only 1/3 of the size of typical PTB domain, we explored the possibility to decrease the size of PTB domain and demonstrat...
Article
Somatic gain-of-function (GOF) mutations in phosphatidylinositol-4, 5-bisphosphate 3-kinase catalytic subunit alpha (PIK3CA), the catalytic subunit of phosphoinositide 3-kinase (PI3K), have been recently discovered in cerebral cavernous malformations (CCMs), raising the possibility that the activation of PI3K pathways is a possible universal regula...
Article
Breast cancer is the most diagnosed cancer worldwide and remains the second leading cause of cancer death. While breast cancer mortality has steadily declined over the past decades through medical advances, an alarming disparity in breast cancer mortality has emerged between African American women (AAW) and Caucasian American women (CAW). New evide...
Article
Objective: Triple-negative breast cancer (TNBC) constitutes ∼15% of all diagnosed invasive breast cancer cases with limited options for treatment since immunotherapies that target ER, PR, and HER2 receptors are ineffective. Progesterone (PRG) can induce its effects through either classic, nonclassic, or combined responses by binding to classic nucl...
Preprint
Background : Breast cancer, the most commonly diagnosed cancer, remains the second leading cause of cancer death in the United States, and excessive Progesterone (PRG) or Mifepristone (MIF) exposure, including females under Hormone Replacement Therapy or taking hormonal contraceptives during their reproductive ages, may be at an increased risk for...
Preprint
Background Breast cancer, the most commonly diagnosed cancer, remains the second leading cause of cancer death in the United States, and excessive Progesterone (PRG) or Mifepristone (MIF) exposure, including females under Hormone Replacement Therapy or taking hormonal contraceptives during their reproductive ages, may be at an increased risk for de...
Article
Full-text available
Purpose The objective of this study is to validate the existence of dual cores within the typical phosphotyrosine binding (PTB) domain and to identify potentially damaging and pathogenic nonsynonymous coding single nuclear polymorphisms (nsSNPs) in the canonical PTB domain of the CCM2 gene that causes cerebral cavernous malformations (CCMs). Metho...
Preprint
Purpose The objective of this study is to validate the existence of dual cores within the typical phosphotyrosine binding (PTB) domain and to identify potentially damaging and pathogenic nonsynonymous coding single nuclear polymorphisms (nsSNPs) in the canonical PTB domain of the CCM2 gene that causes cerebral cavernous malformations (CCMs). Metho...
Article
Background: Cerebral cavernous malformations (CCMs) are microvascular CNS lesions prone to hemorrhage leading to neurological sequela such as stroke and seizure. A subset of CCM patients have aggressive disease leading to multiple bleeding events, likely resulting multiple hospitalizations. Hospital admission rates are an important metric that has...
Preprint
Full-text available
BACKGROUND Cerebral cavernous malformations (CCMs) are microvascular CNS lesions prone to hemorrhage leading to neurological sequela such as stroke and seizure. A subset of CCM patients have aggressive disease leading to multiple bleeding events, likely resulting multiple hospitalizations. Hospital admission rates are an important metric that has d...
Preprint
Full-text available
Objective: Triple-negative breast cancer (TNBC) constitutes ~15 percent of all diagnosed invasive breast cancer cases with limited options for treatment since immunotherapies that target the ER, PR and HER2 receptors are ineffective. Progesterone (PRG) is capable of inducing its effects through either classic, non-classic, or combined responses by...
Article
Background Phosphotyrosine Binding (PTB) Domains, usually found on scaffold proteins, are pervasive in many cellular signaling pathways. These domains are the second-largest family of phosphotyrosine recognition domains and since their initial discovery, dozens of PTB domains have been structurally determined. Scope of review Due to its signature...
Preprint
We demonstrate that a novel signaling network among the CSC and mPRS is dynamically modulated and fine-tuned with intricate feedback regulations in PR negative cells, especially endothelial cells (ECs). Depletion of any of three CCMs (1, 2, 3) genes results in the disruption of non-classic mPRs -mediated signaling in-vitro as well as defective home...
Chapter
Molecular techniques allow for the rapid discovery of CCM-associated protein targets, crucial to understanding CCM pathogenesis. Here, we describe optimized protein extraction methods that allow for extraction from whole cell, and/or cellular sub-compartments, including nuclear, mitochondria, cytoplasmic, and membrane-bound proteins, from lysates....
Chapter
Cellular techniques allow researchers to discover underlying mechanisms of pathogenesis of CCMs in vitro before carrying over into in vivo models; optimization of these techniques facilitates the rapid discovery of CCM-associated gene and protein targets. Here, we describe optimized cell culture applications which are essential for successful molec...
Chapter
The use of vertebrate models allows researchers to investigate mechanisms of CCM pathogenesis in vivo, to investigate discrepancies between observations seen in the lab with in vitro experiments and how they translate into animal models; these in vivo models are more relevant in terms of CCM pathogenesis seen in humans than the in vitro counterpart...
Chapter
Omics research has garnered popularity recently to integrate in-depth analysis of alterations at the molecular level to elucidate observable phenotypes resulting from knockdown/knockout models. Genomics, performed through RNA-seq, allows the user to evaluate alterations at the transcription level, oftentimes more sensitive than other types of analy...
Preprint
Full-text available
Excessive progesterone (PRG) may increase breast cancer risk under hormone therapy during postmenopause or hormonal contraception. As a sex steroid hormone, PRG exerts its cellular responses through signaling cascades involving classic (genomic), non-classic (non-genomic), or both combined responses by binding to either classic nuclear PRG receptor...
Article
A numerical model for the temperature field calculation of high-strength concrete pavement is developed in this paper. Cement hydration, solar radiation and atmospheric temperature changes are taken into consideration in the modelling. Temperature field of high-strength concrete pavement made by concrete with a compressive strength of 100 MPa at 28...
Article
Full-text available
Cerebral cavernous malformations (CCMs) are microvascular anomalies in the brain that result in increased susceptibility to stroke. Three genes have been identified as causes of CCMs: cerebral cavernous malformations 1 [(CCM1) also termed Krev interaction trapped 1 (KRIT1)], cerebral cavernous malformation 2 [(CCM2) also termed MGC4607] and cerebra...
Article
Full-text available
Background: Cerebral cavernous malformations (CCMs), a major neurosurgical condition, characterized by abnormally dilated intracranial capillaries, result in increased susceptibility to stroke. KRIT1 (CCM1), MGC4607 (CCM2), and PDCD10 (CCM3) have been identified as causes of CCMs in which at least one of them is disrupted in most familial cases. O...
Article
Full-text available
Cerebral cavernous malformations (CCMs) are characterized by abnormally dilated intracranial capillaries that result in increased susceptibility to stroke. Three genes have been identified as causes of CCMs; KRIT1 (CCM1), MGC4607 (CCM2) and PDCD10 (CCM3); one of them is disrupted in most CCM cases. It was demonstrated that both CCM1 and CCM3 bind t...
Article
Full-text available
Cerebral cavernous malformations (CCMs) is a microvascular disorder in the central nervous system. Despite tremendous efforts, the causal genetic mutation in some CCM patients has not be identified, raising the possibility of an unknown CCM locus. The CCM2/MGC4607 gene has been identified as one of three known genes causing CCMs. In this report, we...
Preprint
Cerebral cavernous malformations (CCMs) are characterized by abnormally dilated intracranial capillaries that result in increased susceptibility to stroke. Three genes have been identified as causes of CCMs; KRIT1 (CCM1), MGC4607 (CCM2) and PDCD10 (CCM3); one of them is disrupted in most CCM cases. It was demonstrated that both CCM1 and CCM3 bind t...
Article
Full-text available
Alternatively translated from the ENO gene and expressed in an array of vertebrate and plant tissues, c-Myc binding protein 1 (MBP-1) participates in the regulation of growth in organisms, their development and their environmental responses. As a transcriptional repressor of multiple proto-oncogenes, vertebrate MBP-1 interacts with other cellular f...
Article
Full-text available
Cerebral cavernous malformations (CCM) are manifested by microvascular lesions characterized by leaky endothelial cells with minimal intervening parenchyma predominantly in the central nervous system predisposed to hemorrhagic stroke, resulting in focal neurological defects. Till date, three proteins are implicated in this condition: CCM1 (KRIT1),...
Article
Background: Three NPXY motifs and one FERM domain in CCM1 makes it a versatile scaffold protein for tethering the signaling components together within the CCM signaling complex (CSC). The cellular role of CCM1 protein remains inadequately expounded. Both phosphotyrosine binding (PTB) and pleckstrin homology (pH) domains were recognized as structur...
Article
Full-text available
Background: Stroke is the fourth leading cause of death in US. Amongst other factors such as age, sex, race, genetics, obesity, diabetes etc., hypertension continues to be the leading contributing factor towards stroke. Studies regarding stroke in Hispanics are sparse and inconclusive. Objectives: The objective of the present study is to investi...
Article
In this paper, shrinkage and internal relative humidity of internal cured with calcinated zeolite particles of high strength engineerined cementitious composite (HSECC) are experimentally investigated, in which the natural sand-like zeolite particles with average particle size of 0.18 mm were used as internal curing agent. Impacting factors, such a...
Article
Background: Isoflurane has been demonstrated to limit myocardial ischemic injury. This effect is hypothesized to be mediated in part via effects on mitochondria. We investigated the hypothesis that isoflurane maintains mitochondrial respiratory chain functionality, in turn limiting mitochondrial damage and mitochondrial membrane disintegration dur...
Article
Fracture mechanics based flexural model for layered engineered cementitious composite (ECC)-concrete beam is proposed. With the model, the flexural behavior of the layered beam is simulated. The model results show that flexural strength improvement depends on the strength of concrete and ECC, as well as the thickness of ECC. As the ECC thickness re...
Article
Metabolomics investigations hold promise for the characterization of small molecules, metabolites, which govern the ultimate manifestation of cardiac phenotypes. In this study, we employed a mass spectrometry-based metabolomics approach to identify metabolic marker(s), which dynamically reflect the cardiac performance of heart failure patients amid...
Article
The Hispanic population carries a disproportionate burden of stroke compared with the non-Hispanic White population. Most studies have been conducted on Caribbean Hispanics, indicating a need to better understand the characteristics of stroke and its prevalence among the Hispanic populations of Mexican descent. In this report, data were collected i...
Article
Textile reinforced cementitious composite board was produced by a sprinkling water hardening technique. The effect of textile ratio and location manner of textile on the flexural performance of the board is investigated. Experimental results show that the flexural behaviors of the board made with sprinkling water hardening technique are greatly imp...
Article
Experimental studies on shrinkage and interior humidity of three series of concretes with three aggregate content in each concrete under sealing and drying conditions were carried out. The results show that the development of interior humidity of concrete is influenced strongly by water-binder ratio and curing status of the specimen. Under drying c...
Article
In the paper, the moisture content, in terms of relative humidity, inside of high strength concrete slab, with and without addition of pre-soaked lightweight aggregate (PSLWA), exposed to normal indoor environment is investigated by continually measuring the interior humidity of concrete immediately after slab casting until 28 days. The effects of...
Article
Full-text available
Jojoba (Simmondsia chinensis) is mainly distributed in desert, and the molecular mechanisms of jojoba in response to abiotic stress still remain elusive. In this paper, we cloned and characterized a SOD gene from jojoba named as ScMnSOD, and introduced into Arabidopsis to investigate its functions of responding to drought stress. The transgenic Ara...
Article
Mitochondria are a common energy source for organs and organisms; their diverse functions are specialized according to the unique phenotypes of their hosting environment. Perturbation of mitochondrial homeostasis accompanies significant pathological phenotypes. However, the connections between mitochondrial proteome properties and function remain t...
Article
In this study, shrinkage induced cracking performance of low shrinkage engineered cementitious composite (LSECC) and traditional ECC is evaluated comparatively by ring tests. The development of free shrinkage and interior humidity of LSECC and traditional ECC under plastic film sealed and drying conditions were experimentally measured. The anti-cra...
Article
Influence of substituting volume fraction of pre-wetted light weight aggregate (LWA) on the early-age free shrinkage, internal relative humidity and elastic modulus of four high strength concrete (HSC), with induced curing water to cement ratios of 0, 0.04, 0.08, 0.12 and with a similar compressive strength, under plastic film sealing and environme...
Article
This paper presents an experimental study on the potential applications of the fiber reinforced engineered cementitious composite with characteristic of low drying shrinkage (LSECC) in concrete pavements for the purpose of eliminating joints that are normally used to accommodate temperature and shrinkage deformation. It is found that a composite sl...
Article
An adiabatic temperature rise (ATR) test was used to determine the degree of cement hydration in concrete. In the experiments, emphasis was placed on the influences of water to cementitious material ratio (w/b) and aggregate content on the ATR and the development of degree of cement hydration. A total of nine concrete mixtures with three w/b ratios...
Article
In this paper, the ring test is used to determine the stress relaxation parameters of concrete in tension at early-age. Through ring test, the steel–concrete interfacial compressive stress generated from shrinkage of concrete is obtained first. Meanwhile, the theoretical value of the interfacial compressive stress is precisely calculated by a model...
Article
To investigate the effect of volume fraction of coarse aggregate ( VFCA) on the developments of elastic modulus and prismatic compressive strength of early-age concrete,mixtures with different VFCA were designed for concretes with water to cementitious material ratio ( w /b) of 0. 62,0. 43 and 0. 3 respectively.Keeping the mortar composition and w...
Article
Full-text available
Background and Purpose. Tornwaldt's cysts are rare nasopharyngeal lesions that develop from remnants of the embryonic notochord. Summary of Case. We reported a twelve-year-old female stroke patient with Tornwaldt's cysts, whose father also suffered a stroke at age fifty two with the presence of an abdominal aortic aneurysm, suggesting a genetic inf...
Article
The dry–wet cycle is one of the aggressive environmental conditions suffered by concrete. This article focuses on the experimental study and theoretical simulation of shrinkage of concrete during dry–wet cycles. The experimental results show that the interior relative humidity of concrete is periodically changed during dry–wet cycles. As concrete u...
Article
Mitochondrial protein homeostasis is an essential component of the functions and oxidative stress responses of the heart. To determine the specificity and efficiency of proteome turnover of the cardiac mitochondria by endogenous and exogenous proteolytic mechanisms. Proteolytic degradation of the murine cardiac mitochondria was assessed by 2-dimens...
Article
Over-expression of Bcl-2 protects against myocardial ischemia/reperfusion (I/R) injury. Nevertheless, the participation of Bcl-2 in basal myocardium, and its subcellular targets under such conditions, remains elusive. Using a mouse line with an ablation of the Bcl-2 gene, we found that myocardial infarct size (IS) was exacerbated vs. wild type (WT)...
Article
The mitochondrial respiratory chain is a collection of five multi-protein complexes, whose unobstructed functionality represents a pivotal element at the crossroads of cell death or survival. However, its molecular composition and stochiometric information remains elusive and the adaptive abilities of the chain remain largely unknown. We employed a...
Article
Full-text available
Cerebral cavernous malformation is a clinically well-defined microvascular disorder predisposing to stroke; however, the major phenotype observed in zebrafish is the cardiac defect, specifically an enlarged heart. Less effort has been made to explore this phenotypic discrepancy between human and zebrafish. Given the fact that the gene products from...
Article
Mode I crack propagation in fiber reinforced cementitious composite is simulated based on fracture mechanics criteria. To analyze crack propagation, a superposition method is employed to calculate the stress intensity factor at the crack tip resulted from both the applied load and the crack bridging stress. Using the model, the effects of various m...
Article
Full-text available
This article reviews basic concepts, general applications, and the potential impact of next-generation sequencing (NGS) technologies on genomics, with particular reference to currently available and possible future platforms and bioinformatics. NGS technologies have demonstrated the capacity to sequence DNA at unprecedented speed, thereby enabling...
Article
Full-text available
This study evaluated changes in DNA methylation in Arabidopsis thaliana plants grown from seeds implanted with low-energy N(+) and Ar(+) ions. Methylation-sensitive amplified polymorphism (MSAP) testing revealed altered DNA methylation patterns after ion implantation at doses of 1 × 10(14) to 1 × 10(16) ions/cm(2). Comparison of the MSAP electropho...
Article
Polymer-based conductive adhesive materials have become widely used in many electronic packaging interconnect applications. Uniaxial ratchetting behavior of anisotropic conductive adhesive film (ACF) was studied by uniaxial cyclic tension experiments. The effects of mean stress, stress amplitude and loading history on the ratchetting response of AC...
Article
Full-text available
Cerebral cavernous malformations (CCMs) are characterized by abnormally dilated intracranial capillaries that have a propensity to bleed. The development of some CCMs in humans has been attributed to mutations in CCM1 and CCM2 genes. In animal models, major cardiovascular defects caused by both gene mutations have been observed. However, the effect...
Article
Hereditary CCMs have been found to be caused by a loss-of-function mutation in one of three CCM genes: KRIT1 (CCM1), MGC4607 (malcavernin, CCM2), and PDCD10 (CCM3). Mutations seen with KRIT1 in pedigrees with CCM1 were the first to be found [1–4]. Subsequently, MGC4607, a phosphotyrosine-binding protein, was identified linked to the CCM2 locus [5],...
Article
Full-text available
High salinity stress is a major environmental factor that limits plant’s distribution and productivity. An Arabidopsis-related halophyte, Thellungiella halophila, is an emerging model system used for plant abiotic stress tolerance research. Previous studies have suggested that protein phosphorylation has a crucial role in the high salinity response...
Article
In this study, an inverse analysis approach is developed to obtain the fracture parameters of concrete, including stress–crack opening relationship, cracking and tensile strength as well as fracture energy, from the results of a three-point bending test. Using this approach, the effects of coarse aggregate size (5–10, 10–16, 16–20 and 20–25mm) and...
Article
Full-text available
Cerebral cavernous malformations (CCM) are characterized by abnormal dilated intracranial capillaries that predispose to hemorrhage. The development of some CCMs in humans has been attributed to mutations in the CCM1 genes. Currently, contradictory results have been generated regarding the vascular endothelial cell population changes in Ccm1 defici...
Article
Essential tremor (ET), one of the most common neurological disorders, comprises uncontrollable tremor, usually in the upper extremities. Idiopathic normal pressure hydrocephalus (iNPH) features ventricular enlargement in the absence of elevated intracranial pressure; its three cardinal symptoms are gait impairment, incontinence, and dementia. iNPH...
Article
Full-text available
Cavernous malformations (CMs) consist of dilated vascular channels that have a characteristic appearance on MRI. CMs are usually found intracranially, although such lesions can also affect the spinal cord. Individuals with CMs can present with epilepsy and focal neurological deficits or acute intracranial hemorrhage. In many cases, however, patient...
Article
In order to study the impact of incident ions on organism mutation, positively and negatively charged low-energy oxygen and hydrogen ions were implanted into the dry seeds of Arabidopsis thaliana using three different dosages. The effects of ion irradiation on peroxidase abundance, activity, isozyme patterns, and transcription were analyzed. The re...
Article
Despite massive efforts, progress so far has been modest in isolating the genetic determinants for intracranial aneurysm (IA). More detailed epidemiology data might be essential for successful genome-wide association study. Here, we aimed to investigate epidemiology and identify the key risk factors associated with the pathogenesis of IA in a large...
Article
To evaluate and identify the risk factors associated with the pathogenesis of congenital hydrocephalus in a large specific population. An International Classification of Diseases (ICD)-9 database search of patients with congenital hydrocephalus treated at the University of Mississippi Medical Center between 1998 and 2007 was performed. All recruite...
Article
Intracranial aneurysms (IAs) are the dilatations of blood vessels in the brain and pose potential risk of rupture leading to subarachnoid hemorrhage. Although the genetic basis of IAs is poorly understood, it is well-known that genetic factors play an important part in the pathogenesis of IAs. Therefore, the identifying susceptible genetic variants...
Article
In this case report, we describe two siblings with a previously unreported partial monosomy 4q and partial trisomy 9q. The sibling karyotypes were determined to be 46,XX,der(4)t(4;9)(q33;q33)pat and 46,XY,der(4)t(4;9)-(q33;q33)pat. The siblings share several common pathological features, including VSD, PDA, low-set ears and digit anomalies as well...
Patent
There are disclosed therapeutic compositions and methods using isolated nucleic acid molecules encoding a human myeloid progenitor inhibitory factor-1 (MPIF-1) polypeptide (previously termed MIP-3 and chemokine β8 (CKβ8 or ckb-8)); a human monocyte-colony inhibitory factor (M-CIF) polypeptide (previously termed MIP1-γ and chemokine β1 (CKβ1 or ckb-...
Article
In order to study the workability and establish the optimum hot forming processing parameters for 42CrMo steel, the compressive deformation behavior of 42CrMo steel was investigated at the temperatures from 850 °C to 1150 °C and strain rates from 0.01 s−1 to 50 s−1 on Gleeble-1500 thermo-simulation machine. Based on these experimental results, an a...
Article
A method toward unsupervised segmentation of synthetic aperture radar (SAR) images is proposed. In this method, the distribution of SAR intensity image and the maximum a posteriori (MAP) algorithm is used to obtain an initial segmentation. Then according to the equivalence between the solid heat diffusion model and image scale-space, multiscale ani...
Article
Using ribonucleic acid interference on cultured cell lines, we examined the role of Krev interaction trapped 1 (krit1) and integrin cytoplasmic domain-associated protein-1 alpha (icap1alpha) in beta1-integrin-mediated cell proliferation. Upon depletion of either krit1 or icap1alpha in the HeLa cells, umbilical vein endothelial cells, and microvascu...
Article
New interconnect materials are always necessary as a result of evolving packaging technologies and increasing performance and environmental demands on electronic systems. Polymer-based conductive-adhesive materials have become widely used in many electronic packaging interconnect applications. Among all the conductive-adhesive materials, the anisot...
Article
In this report, we identified a large five-generation distinctive kindred with essential tremor (ET) presenting during the teen years and the consequent appearance of idiopathic normal pressure hydrocephalus (iNPH) when elderly (>65 years), in an autosomal dominant fashion. Through clinical and genetic analysis, we defined this kindred as a new ess...