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23
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Introduction
Current institution
Additional affiliations
January 2017 - January 2017
December 2013 - present
December 2012 - November 2013
Education
March 2005 - March 2007
July 2000 - January 2005
Publications
Publications (23)
Due to the recent taxonomic reclassification of the species Flavobacterium columnare into four new species—Flavobacterium columnare, Flavobacterium davisii, Flavobacterium covae, and Flavobacterium oreochromis—it is necessary to re-evaluate isolates of previous outbreaks to better understand the epidemiology related to this bacterial group. Therefo...
The Amazonian manatee (Trichechusinunguis) is an aquatic mammal threatened with extinction. However, few studies have investigated the pathogens in this species, which may affect both animal and human health. This study aimed to evaluate the frequency, distribution, and patterns of antimicrobial susceptibility of Staphylococcus spp. and Escherichia...
Lactococcus petauri is an emergent fish pathogen detected in mortality outbreaks in Nile tilapia and rainbow trout farms on the American continent. Diseased fish exhibits erratic swimming, eye disorders, dark skin pigmentation among other clinical signs. The multilocus sequence typing (MLST) is a well-established methodology for the study of bacter...
Piscine lactococcosis is a disease caused by Lactococcus garvieae, L. petauri and L. formosensis. It is considered to be an emerging disease and poses a risk to global aquaculture production. Studies of the genetic diversity of Lactococcus spp. strains have epidemiological importance since they provide information on the genetic relationships betwe...
Piscine lactococcosis is a disease caused by Lactococcus garvieae, L. petauri and L. formosensis. It is considered to be an emerging disease and poses a risk to global aquaculture production. Studies of the genetic diversity of Lactococcus spp. strains have epidemiological importance since they provide information on the genetic relationships betwe...
Lactococcosis in fish has been associated with Lactococcus garvieae and the recently described L. petauri. However, the relevance of these emerging fish pathogens to Nile tilapia still requires thorough understanding. This study investigated lactococcosis outbreaks in Nile tilapia on Brazilian farms and characterized the isolates through multilocus...
Lactococcosis in fish has been associated with Lactococcus garvieae and the recently described L. petauri . However, the relevance of these emerging fish pathogens to Nile tilapia still requires thorough understanding. This study investigated lactococcosis outbreaks in Nile tilapia on Brazilian farms and characterized the isolates through molecular...
Dengue virus (DENV) 1-4 is the etiological agent of dengue, the most important viral infection transmitted by Aedes spp mosquitoes to humans. Our goal was to identify the circulating DENV in Aedes aegypti collected in an area of Brazil where all four DENV serotypes had already been detected in humans, understand the epidemiology better, and to test...
In June 2020, an atypical fatal outbreak in a Brazilian Nile tilapia farm was investigated. Twenty-three animals were collected and different tissues were used for bacterial isolation, histopathological and electron microscopic examination, and viral detection using molecular methods. A large number of megalocytes were observed in the histopatholog...
One of the major challenges in Nile tilapia (Oreochromis niloticus L.) farming is the occurrence of bacterial infections, and the Francisella noatunensis subsp. orientalis (FNO) is an important pathogen that has emerged in last decades. Francisellosis outbreaks have been reported in the literature as occurring seasonally when water temperature is b...
Bela Vista Biological Sanctuary (RBV) is a protected area of Itaipu Binacional, a hydroelectric power company located on the border of Brazil and Paraguay. A captive population of Brazilian dwarf brocket deer (Mazama nana, Cervidae, Artiodactyla) is maintained for conservation purposes. Despite the reproductive success of the animals, outbreaks of...
Bluetongue (BT) is a vector-borne viral disease caused by the Bluetongue virus (BTV), an Orbivirus from the Reoviridae family, affecting domestic and wild ruminants. BTV circulation in Brazil was first reported in 1978, and several serological surveys indicate that the virus is widespread, although with varied prevalence. In 2014, BT outbreaks affe...
The complete genome sequence of Bluetongue virus (BTV) serotype 17 strain 17/BRA/2014/73, isolated from a sheep in Brazil in 2014, is reported here. All segments clustered with western topotype strains and indicated reassortment events with other BTV from the Americas. The strain 17/BRA/2014/73 represents a novel reference strain for BTV-17 from So...
In January 2013, an outbreak of Bluetongue (BT) affecting a Lacaune sheep flock occurred in Vassouras, Rio de Janeiro state, Brazil. From March to August 2013, blood samples collection and clinical examination were performed monthly, in order to monitor the epidemiological profile of Bluetongue virus (BTV) circulation and clinical disease in the fl...
Dengue is currently regarded as the major public health problem worldwide. In a hyperendemic region during an outbreak, we detected the co-circulation of all dengue virus (DENV) serotypes including two different genotypes DENV-3 and DENV-4, and concurrent infections with up to three serotypes were identified in symptomatic patients. A total of 49 a...
Yeast cells need to respond to a variety of stresses found in such different conditions as gastrointestinal tract after probiotic ingestion or fermentation vat during ethanol production. In the present study, H + neutralisation capacity, membrane fatty acid composition, H + -ATPase activity, and cytosolic Ca 2+ concentration were evaluated in yeast...
In several organisms used for recombinant protein production, integration of the expression cassette into the genome depends on site-specific recombination. In general, the yeast Kluyveromyces lactis shows low gene-targeting efficiency. In this work, two K. lactis
ku80
−
strains defective in the non-homologous end-joining pathway (NHEJ) were constr...
Yeast cells need to respond to a variety of stresses found in such different conditions as gastrointestinal tract after probiotic ingestion or fermentation vat during ethanol production. In the present study, H + neutralisation capacity, membrane fatty acid composition, H +-ATPase activity, and cytosolic Ca 2+ concentration were evaluated in yeast...
L-ascorbic acid (L-AA) is naturally synthesized in plants from D-glucose by 10 steps pathway. The pathway branch to synthesize L-galactose, the key intermediate for L-ascorbic acid biosynthesis, has been recently elucidated. Budding yeast produces an 5-carbon ascorbic acid analogue Dehydro-D-arabinono 1,4-lactone (D-DAL), which is synthesized from...
The high affinity interaction streptavidin-biotin provides an excellent system for industrial enzyme separation or immobilization involving a fused streptavidin-enzyme and a biotinylated support. In order to produce proteins with affinity to biotin, we have modified the commercial K. lactis system, pKLAC1 (New England Biolabs), a LAC4 promoter-driv...
