Julio Castaneda

Julio Castaneda
Osaka University | Handai · Department of Experimental Genome Research

Ph.D.

About

28
Publications
2,714
Reads
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717
Citations
Citations since 2016
14 Research Items
504 Citations
2016201720182019202020212022020406080100120
2016201720182019202020212022020406080100120
2016201720182019202020212022020406080100120
2016201720182019202020212022020406080100120
Additional affiliations
October 2017 - present
Osaka University
Position
  • Professor (Assistant)
July 2013 - July 2016
Baylor College of Medicine
Position
  • PostDoc Position
September 2012 - June 2013
Carnegie Institution for Science
Position
  • PostDoc Position
Education
July 2005 - October 2012
Johns Hopkins University
Field of study
  • Biology
September 2002 - March 2005
University of California, Davis
Field of study
  • Biotechnology

Publications

Publications (28)
Article
Full-text available
Meiosis has a principal role in sexual reproduction to generate haploid gametes in both sexes. During meiosis, the cell nucleus hosts a dynamic environment where some genes are transcriptionally activated, and some are inactivated at the same time. This becomes possible through subnuclear compartmentalization. The sex body, sequestering X and Y chr...
Article
Full-text available
Infertility afflicts up to 15% of couples globally each year with men a contributing factor in half of these cases. Globozoospermia is a rare condition found in infertile men that is characterized by defective acrosome biogenesis leading to the production of round shaped sperm. Here, we report a novel gene, Fam209 (Family with sequence similarity 2...
Article
Full-text available
The flagellum is essential for sperm motility and fertilization in vivo. The axoneme is the main component of the flagella, extending through its entire length. An axoneme is comprised of two central microtubules surrounded by nine doublets, the nexin-dynein regulatory complex, radial spokes, and dynein arms. Failure to properly assemble components...
Article
Full-text available
Developing a safe and effective male contraceptive remains a challenge in the field of medical science. Molecules that selectively target the male reproductive tract and whose targets are indispensable for male reproductive function serve among the best candidates for a novel non-hormonal male contraceptive method. To determine the function of thes...
Article
CRISPR/Cas9-based genome editing in mice uncovers 13 testis-or epididymis-enriched genes individually dispensable for male reproduction Running title: Genome editing reveals dispensable testis-and epididymis-enriched genes Summary sentence: Thirteen testis-or epididymis-enriched genes are individually dispensable for male fertility based on phenoty...
Article
Full-text available
Spermatogenesis is a complex developmental process that involves the proliferation of diploid cells, meiotic division, and haploid differentiation. Many genes are shown to be essential for male fertility using knockout (KO) mice; however, there still remain genes to be analyzed to elucidate their molecular mechanism and their roles in spermatogenes...
Article
Full-text available
In mammals, more than 2,000 genes are specifically or abundantly expressed in testis, but gene knockout studies revealed several are not individually essential for male fertility. Tesmin (Metallothionein-like 5; Mtl5) was originally reported as a testis-specific transcript that encodes a member of the CXC motif containing metallothionein family. La...
Article
Full-text available
The t-complex is defined as naturally occurring variants of the proximal third of mouse chromosome 17 and has been studied by mouse geneticists for decades. This region contains many genes involved in processes from embryogenesis to sperm function. One such gene, t-complex protein 11 (Tcp11), was identified as a testis-specific gene whose protein i...
Article
Full-text available
More than 1,000 genes are predicted to be predominantly expressed in mouse testis, yet many of them remain unstudied in terms of their roles in spermatogenesis and sperm function and their essentiality in male reproduction. Since individually indispensable factors can provide important implications for the diagnosis of genetically-related idiopathi...
Article
Full-text available
Significance Infertility is a global problem that afflicts 15% of couples, and in 50% of cases, the attributing factor is linked to men. Among these infertile men, 18% specifically exhibit decreased motility of sperm (asthenozoospermia). Sperm motility is dependent on the formation and functioning of the flagellum, a modified cilium used for locomo...
Article
Full-text available
Significance In the mouse genome, thousands of genes are predominantly expressed in the testis, where these genes are thought to play important roles in spermatogenesis and fertilization. However, in this study, we report that 54 evolutionarily conserved and testis-enriched genes are not essential individually for male mouse fertility. Because the...
Article
Full-text available
The population of our planet continues to rise at a rapid rate and is reaching unsustainable numbers. The Strategic Plan 2000 of the National Institute of Child Health and Human Development (NICHD) states that uncontrolled fertility “is one of the most pressing public health challenges facing the world today” ( 1 ). However, while women have had th...
Article
Full-text available
Pachytene piRNAs are a class of Piwi-interacting small RNAs abundant in spermatids of the adult mouse testis. They are processed from piRNA primary transcripts by a poorly understood mechanism and, unlike fetal transposon-derived piRNAs, lack complementary targets in the spermatid transcriptome. We report that immunopurified complexes of a conserve...
Article
Integrity of the germline genome is essential for the production of viable gametes and successful reproduction. In mammals, the generation of gametes involves extensive epigenetic changes (DNA methylation and histone modification) in conjunction with changes in chromosome structure to ensure flawless progression through meiotic recombination and pa...
Article
Epigenetic reprogramming of embryonic mouse germ cells involves DNA demethylation of the genome that is accompanied by derepression of transposable elements (TEs). Threatening the genome's integrity, TE activation is efficiently countered by the concerted action of de novo DNA methylation and PIWI-interacting small RNAs (piRNAs). Recent studies hav...
Data
Co-localization of P-body components in the Mael-mutant gonocytes. In Mael-deficient gonocytes, XRN-1, GW182, and DCP1a co-localization remains intact. (4.93 MB TIF)
Data
Analysis of gonocyte cell cycle arrest in wild-type and Mael-mutant animals by EdU labeling of replicating DNA. (A) Schematic outline of the experiment–timing of EdU injections in two groups of animals and their sacrifice. (B) Representative DAPI/EdU/MVH staining of processed gonadal tissues. No EdU labeling was observed in gonocytes. (C) Quantific...
Data
GW182 forms an outer shell of the piP-body. (2.16 MB TIF)
Data
Electron micrograph images of piP-bodies in wild-type and Mael-mutant gonocytes. Examples of piP-bodies in wild-type (A) and in Mael-mutant (B) gonocytes. Regions indicated in red boxes are shown magnified below the overview. (5.89 MB TIF)
Data
MILI localization in Mael-mutant gonocytes. Loss of MAEL does not affect the localization of MILI. (2.45 MB TIF)
Data
Associations of MVH and DCP1a in wild-type and Mael-mutant gonocytes. In wild-type gonocytes, 3 types of MVH-DCP1a localizations were observed: 1) overlapping, 2) associated granules, or 3) solitary granules. In the Mael mutant, virtually only associated or solitary DCP1a granules were present. (1.18 MB TIF)
Data
Size distributions of LINE and SINE small RNAs from E16.5 Mael-mutant testes. (0.46 MB TIF)
Data
Results of small RNA sequencing. (0.14 MB TIF)
Data
DNMT3L and DNMT3A2 expression in E16.5 Mael-mutant gonocytes. At day E13.5, no DNMT3L and DNMT3A2 staining was observed in wild-type and Mael-mutant gonocytes (data not shown). At day E16.5, all gonocytes showed prominent nuclear staining. (3.82 MB TIF)
Article
Full-text available
Derepression of transposable elements (TEs) in the course of epigenetic reprogramming of the mouse embryonic germline necessitates the existence of a robust defense that is comprised of PIWI/piRNA pathway and de novo DNA methylation machinery. To gain further insight into biogenesis and function of piRNAs, we studied the intracellular localization...

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