Julie Tottey

Julie Tottey
French National Institute for Agriculture, Food, and Environment (INRAE) | INRAE · Infectiologie et Santé Publique

PhD

About

23
Publications
2,781
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875
Citations
Introduction
Intracellular parasites are one of the most fascinating living creatures and I am interested in dissecting the mechanisms they use to adapt to their hosts. During my training in France and in the UK, I studied microsporidian and apicomplexan parasites. My work now focuses on the intestinal parasite Cryptosporidium parvum. My project aims at dissecting strategies developed by this parasite to achieve its life cycle inside epithelial cells using molecular biology and genetic manipulation.
Additional affiliations
February 2016 - present
French National Institute for Agriculture, Food, and Environment (INRAE)
Position
  • Researcher
February 2014 - December 2015
University of Glasgow
Position
  • Research Associate
September 2010 - December 2013
Université Clermont Auvergne
Position
  • PhD Student

Publications

Publications (23)
Article
Full-text available
Background: The honeybee, Apis mellifera, is undergoing a worldwide decline whose origin is still in debate. Studies performed for twenty years suggest that this decline may involve both infectious diseases and exposure to pesticides. Joint action of pathogens and chemicals are known to threaten several organisms but the combined effects of these...
Article
Full-text available
In ecosystems, a variety of biological, chemical and physical stressors may act in combination to induce illness in populations of living organisms. While recent surveys reported that parasite-insecticide interactions can synergistically and negatively affect honeybee survival, the importance of sequence in exposure to stressors has hardly received...
Article
Full-text available
Honeybees (Apis mellifera) are constantly exposed to a wide variety of environmental stressors such as parasites and pesticides. Among them, Nosema ceranae and neurotoxic insecticides might act in combination and lead to a higher honeybee mortality. We investigated the molecular response of honeybees exposed to N. ceranae, to insecticides (fipronil...
Article
Full-text available
The microsporidian parasite Nosema ceranae is a common pathogen of the Western honeybee (Apis mellifera) whose variable virulence could be related to its genetic polymorphism and/or its polyphenism responding to environmental cues. Since the genotyping of N. ceranae based on unique marker sequences had been unsuccessful, we tested whether a multilo...
Article
Genetic diversity of a host species is a key factor to counter infection by parasites. Since two separation events and the beginning of beekeeping, the Western honeybee, Apis mellifera, has diverged in many phylogenetically-related taxa that share common traits but also show specific physiological, behavioural and morphological traits. In this stud...
Article
Full-text available
Apicomplexan parasites cause diseases such as malaria and toxoplasmosis. The apicomplexan mitochondrion shows striking differences from common model organisms, including in fundamental processes such as mitochondrial translation. Despite evidence that mitochondrial translation is essential for parasites survival, it is largely understudied. Progres...
Preprint
Full-text available
Apicomplexan parasites cause diseases such as malaria and toxoplasmosis. The apicomplexan mitochondrion shows striking differences from common model organisms, including in fundamental processes such as mitochondrial translation. Despite evidence that mitochondrial translation is essential for parasites survival, it is largely understudied. Progres...
Article
Full-text available
Apicomplexan parasites are global killers, being the causative agents of diseases like toxoplasmosis and malaria. These parasites are known to be hypersensitive to redox imbalance, yet little is understood about the cellular roles of their various redox regulators. The apicoplast, an essential plastid organelle, is a verified apicomplexan drug targ...
Data
Sequence alignment of ATrx2 homologues. Apicomplexans (Api), chromerids (Chr), cryptophytes (Cry), haptophytes, (Hap), heterokonts (Het), and dinoflagellates (Din) including dinoflagellate taxa containing heterokont-derived endosymbionts (Din*). N-terminal protein presequences contain predicted bipartite targeting sequences consistent with plastid...
Data
Recombinant expression and isolation of ATrx2 from bacteria. A. His-tagged ATrx2 expression from the pET28a was induced with Isopropyl β-D-1-thiogalactopyranoside (IPTG) for 12 hours at 16°C and analysed by western blot. Wcl–whole cell lysate. Sol–soluble fraction. B. recombinant ATrx2 was isolated from inclusion bodies and refolding, re-solubilise...
Data
High-resolution microscopy of the co-staining of ATrx1 and ATrx2 with compartmental markers. ATrx1 (top 3 panels, yellow) and of ATrx2 (middle 3 panels, yellow) co-stained with the luminal marker CPN60 (i, iv); with the outer-membrane marker 201270 (ii, v); or with the PPC marker PPP1 (iii, vi) all in magenta. (vii) shows co-staining of ATrx1 (mage...
Data
Primers used in this study. The numbers, names and sequences of each primer used in this study are detailed. The purpose column summarizes what was amplified with each pair of primers. (PDF)
Data
Mass spectrometry data from the six ATrx2 substrate trap experiments. Each sheet contains the list of T. gondii proteins identified in each experiment. For each identified protein the peptides that were found are listed along with their scores. (XLSX)
Data
Super resolution microscopy of the ER retention of ATrx1CXXA. Three sections of the same image of a single parasite transiently expressing Ty1 tagged ATrx1cxxa (magenta) and the ER marker Der1-GFP (yellow). The merge image includes DAPI (blue). The inset and arrow highlight point of co-localization between ATrx1cxxa and Der1-GFP. (TIF)
Data
Analysis of apicoplast biogenesis pathways upon ATrx1 or ATrx2 depletion. A. Western blot analyses analyzing the maturation of the PPC (PPP1) or luminal (LytB) apicoplast proteins that are transiently expressed for 24 hours at each time point of ATrx1 (i, ii) or ATrx2 (iii, iv) depletion. The bar-graph under each western shows the calculated ratio...
Data
Includes detailed description of the in vitro insulin turbidity assay and the Recombinant protein expression and isolation. (PDF)
Article
Primers used in this study. The numbers, names and sequences of each primer used in this study are detailed. The purpose column summarizes what was amplified with each pair of primers. (PDF)
Article
Apis mellifera is a pollinator insect playing of major economical and ecological importance. For more than two decades, severe honeybee colony losses have been reported worldwide. The origin of this phenomenon is thought to involve numerous stressors that could interact with each other. The objective of this work was to evaluate the impact on honey...
Thesis
Apis mellifera est un insecte pollinisateur jouant un rôle économique et écologique majeur. Depuis plus d’une vingtaine d’années, d’importantes pertes de colonies d’abeilles ont été recensées à l’échelle mondiale. L’origine de ce phénomène impliquerait de nombreux facteurs de stress qui pourraient en outre interagir entre eux. Ce travail de thèse a...
Article
Full-text available
SUMMARY The microsporidian parasite Nosema ceranae is a common pathogen of the Western honeybee (Apis mellifera) whose variable virulence could be related to its genetic polymorphism and/or its polyphenism responding to environmental cues. Since the genotyping of N. ceranae based on unique marker sequences had been unsuccessful, we tested whether a...
Data
Full-text available
Supplementary Table S1

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