Juliane Sanft

• Universitätsklinikum Jena

Background COVID-19 now exists for more than 3 years and has caused almost 7 million deaths worldwide. At the beginning of this study only little was known on the patients’ characteristics and comparative autopsy studies are still rare. Material and methods Between 11 March 2020 and 10 March 2021, 55 consecutive and complete autopsies of individua...

Background: In patients diagnosed with cervical cancer, the purpose of lymphadenectomy is the removal of lymph nodes for diagnosis and potential treatment of metastasized tumor cells. It is unclear if afferent lymphatic vessels harbor tumor cells and, thus, may pose additional risk for recurrence or progression if not removed. Aim: In this feasibil...

Clinical observations indicate that COVID-19 is a systemic disease. An investigation of the viral distribution within the human body and its correlation with tissue damage can aid in understanding the pathophysiology of SARS-CoV-2 infection. We present a detailed mapping of the viral RNA in 61 tissues and organs of 11 deceased patients with COVID-1...

Introduction Research on human placental development and function lacks a conclusive in vivo model. To investigate the intracellular molecular mechanisms in trophoblast cells, different cell lines have been established during the last decades. So far, none of these accomplishes all features of primary trophoblast, thus their suitability as well as...

Aims Haemophagocytosis in the bone marrow of patients who have succumbed to COVID‐19 has not been widely studied. The aim of the present study was to perform morphological analyses and morphometry of haemophagocytosis in bone marrow of patients with severe COVID‐19 and to correlate the findings to the clinical course of the disease. Methods and Re...

Clinical observations indicate that COVID-19 is a systemic disease. An investigation of the viral distribution within the human body in correlation to tissue damage can help understanding the pathophysiology of SARS-CoV-2 infection. We present a detailed mapping of viral RNA in 61 tissues and organs of 11 deceased patients with the diagnosis COVID-...

The measurement of minimal residual disease after hematopoietic stem cell transplantation is crucial for the prevention of hematological relapses. But many methods are limited to certain groups of patients. We present a comparison of two markers which are universally applicable: WT1 expression and chimerism status. We analyzed 1,848 peripheral bloo...

Cadavers can be colonized by a wide variety of bacteria and fungi. Some of these microbes could change the concentration or the metabolic pattern of drugs present in postmortem samples. The purpose of this study was to identify fungi from human postmortem material and to further assess their potential role in the metabolism of drugs. Aliquots of 25...

PCR is a commonly used and highly efficient technique in biomolecular laboratories for specific amplification of DNA. However, successful DNA amplification can be very time consuming and troublesome because many factors influence PCR efficiency. Especially GC-rich DNA complicates amplification because of generation of secondary structures that hind...

Kinship relation and, in particular, paternity probability estimation using a Bayesian approach require the input of a priori probabilities of different hypotheses. In practical case work, a priori probabilities or priors, for short, must often be estimated using only common sense and symmetry arguments because in most cases, there is no evidence-b...

A large number of short tandem repeat (STR) markers spanning the entire human X chromosome have been described and established for use in forensic genetic testing. Due to their particular mode of inheritance, X-STRs often allow easy and informative haplotyping in kinship analyses. Moreover, some X-STRs are known to be tightly linked so that, in com...

The clinical relevance of both minimal residual disease (MRD) and chimerism after hematopoietic stem cell transplantation (HSCT) for the detection of impending relapse has not yet been extensively studied. We investigated MRD in 119 consecutive children (median age, 12 years) with ALL (n=56), AML (n=36), MDS (n=20), or CML (n=7) who underwent bone...