Juliana Risso Pariz

• MSc; PhD student
• PhD Student at University of São Paulo

Standard protocols for clinical in vitro fertilization (IVF) laboratories recommend incubating semen at 37°C in 5% CO2 without strictly specifying which medium should be used or for how long. This study aimed to test the most common different incubation media used in Latin American andrology and micromanipulation laboratories and verify which, if a...

Background: The current outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, named coronavirus disease 19 (COVID-19), is not the first well-known spillover of an animal originated virus to infect humans. However, one of the few to make such a fast jump in a powerful evolutionary shortcut. The incredible pattern of ag...

Objective: To compare cryosurvival rates of human spermatozoa in a prolonged period of cryopreservation. Methods: This retrospective study involved 33 cryopreserved semen samples from patients with cancer, between 2002 and 2011. The semen sample was obtained by masturbation and initial semen analysis was performed. The cryoprotectant solution wa...

Cryopreservation processes can damage spermatozoa and impair structural and functional cell characteristics. Plasma, nuclear membranes, and cellular organelles can suffer from the freeze and thaw process. This study evaluates the protective and stimulant effect of melatonin and caffeine supplementation on the functional characteristics of human spe...

Background: It remains challenging to determine which individuals are likely to benefit from microsurgical correction of subclinical varicocele, as basic semen parameters often do not improve postoperatively. We aimed to develop an easily accessible tool for prognostic stratification of infertile men indicated for microsurgical correction of bilat...

This study describes a new method of microcentrifugation as an improved, viable, cost-effective option to the classical Cytospin apparatus to confirm azoospermia. Azoospermic semen samples were evaluated for cryptozoospermia by a centrifugation method similar to that of World Health Organization guidelines (2010; entire specimen centrifuged at 3000...

This study aimed to evaluate the effects of caffeine supplementation in post-cryopreservation human semen over different incubation periods. After collection by masturbation, 17 semen samples were analysed according to World Health Organization criteria, processed and cryopreserved with TEST-yolk buffer (1 : 1) in liquid nitrogen. After a thawing p...

Electron microscopy of sperm is a complementary tool to semen analysis that can contribute to the clinical interpretation of severe astenozoospermia, teratozoospermia and idiopathic infertility investigation. We report a patient with varicocele, submitted to varicocelectomy, with seminal ultrastructural analysis by electron microscopy.

Intrauterine insemination (IUI) should be the first step when a couple is seeking methods to help and/or assist in the reproductive process, after clinical and microsurgical treatments for male infertility have not resulted in pregnancy. The role of an andrologist is to evaluate, treat and also to improve sperm quality/function in order to enhance...

Rosa Alice Monteiro, Juliana Risso Pariz, Patricia de Campos Pieri, Jorge Hallak. Introdution: The verification of azoospermia is indicated for appropriate orientation reproductive of infertile man. The centrifugation method of cell suspension on slides (Cytospin) is widely used for this purpose, although not always easy to identify isolated sperm...

Introduction: The effect of finasteride 1mg/daily on spermatogenesis and its increased use by young males to treat and to prevent male baldness is of concern for the proportion and widespread of prescriptions by dermatologists unaware of its potential reproductive effects. Methods: We studied 19 male subjectrs who came for an initial andrological e...

Introduction: Marijuana, the popular name for dried Cannabis sativa, is the most consumed drug worldwide. The main compound and most potent psychoactive agent, delta-9-tetrahydrocannabinol ( THC), acts as a negative competitor to the endogenous cannabinoid anandamide, by interacting with specific receptors CNR1 (GeneID 1268) and CNR2 (GeneID 1269)....

Unlabelled: What's known on the subject? and What does the study add? The relationship between high levels of BMI and changes in altered standard semen analysis parameters are described in the literature. However, the functional characteristics of the sperm are essential to complete the evaluation of male infertility. Thus, this study provides imp...

STUDY QUESTION What are the effects of smoking on the functional aspects of the sperm, the levels of lipid peroxidation and the protein profile of seminal plasma in patients with varicocele?

Metformin is an insulin sensitizing agent with beneficial effects in diabetic patients on glycemic levels and in the cardiovascular system. We examined whether the metabolic changes and the vascular dysfunction in monosodium glutamate-induced obese non-diabetic (MSG) rats might be improved by metformin. 16 week-old MSG rats were treated with metfor...