Julia Sudnitsyna

Julia Sudnitsyna
Center for Theoretical Problems of Physicochemical Pharmacology

PhD

About

21
Publications
2,030
Reads
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104
Citations
Citations since 2016
21 Research Items
103 Citations
20162017201820192020202120220102030
20162017201820192020202120220102030
20162017201820192020202120220102030
20162017201820192020202120220102030
Additional affiliations
November 2019 - present
Center for Theoretical Problems of Physicochemical Pharmacology
Center for Theoretical Problems of Physicochemical Pharmacology
Position
  • Senior Researcher
March 2019 - present
Sechenov Institute of Evolutionary Physiology and Biochemistry
Position
  • Researcher
Description
  • PostDoc
August 2013 - March 2019
Sechenov Institute of Evolutionary Physiology and Biochemistry
Position
  • Researcher
Education
September 2013 - September 2016
September 2011 - June 2013
September 2007 - June 2011

Publications

Publications (21)
Article
Full-text available
Nicotinamide riboside (NR) is an effective precursor of nicotinamide adenine dinucleotide (NAD) in human and animal cells. NR supplementation can increase the level of NAD in various tissues and thereby improve physiological functions that are weakened or lost in experimental models of aging or various human pathologies. However, there are also rep...
Article
Full-text available
Hypo- and hyperthermia affect both primary and secondary hemostasis; however, there are controversial data concerning platelet activation and the underlying mechanisms under hypo- and hyperthermia. The discrepancies in the data could be partly explained by different approaches to hemostatic reactions analysis. We applied a new LaSca-TMF laser parti...
Article
Full-text available
Activation of the cAMP pathway by β-adrenergic stimulation and cGMP pathway by activation of guanylate cyclase substantially affects red blood cell (RBC) membrane properties in mammals. However, whether similar mechanisms are involved in RBC regulation of lower vertebrates, especially teleosts, is not elucidated yet. In this study, we evaluated the...
Article
Full-text available
Curcumin is a natural bioactive component derived from the turmeric plant Curcuma longa, which exhibits a range of beneficial activities on human cells. Previously, an inhibitory effect of curcumin on platelets was demonstrated. However, it is unknown whether this inhibitory effect is due to platelet apoptosis or procoagulant platelet formation. In...
Article
Full-text available
Preconditioning is often used in medicine to protect organs from ischemic damage and in athletes to enhance the performances. We tested whether low-dose ammonium preconditioning (AMP) could have a beneficial effect on physical exercises (PE). We used Cardiopulmonary Exercise Testing (CPET) on a treadmill to investigate the effects of low-dose AMP o...
Article
Full-text available
Nicotinamide riboside (NR), a new form of vitamin B3, is an effective precursor of nicotinamide adenine dinucleotide (NAD+) in human and animal cells. The introduction of NR into the body effectively increases the level of intracellular NAD+ and thereby restores physiological functions that are weakened or lost in experimental models of aging and v...
Chapter
Toxic and mechanistic properties of nutraceuticals are not as extensively studied as those of pharmaceuticals. Flow cytometry is among the most popular techniques to investigate the modes and mechanisms of cytotoxic action of chemical compounds, though flow cytometry methods are not suitable for online registration of rapid changes in cell volumes....
Article
Full-text available
Extracellular vesicles (EVs) released by different cell types play an important role in many physiological and pathophysiological processes. In physiological conditions, red blood cell (RBC)-derived EVs compose 4-8% of all circulating EVs, and oxidative stress (OS) as a consequence of different pathophysiological conditions significantly increases...
Preprint
Full-text available
Extracellular vesicles (EVs) released by different cell types play a significant role in many physiological and pathophysiological processes. In physiological conditions, red blood cell (RBC)-derived EVs compose 4–8% of all circulating EVs, and oxidative stress (OS) as a consequence of different pathophysiological conditions significantly increases...
Article
End stage of chronic kidney disease (CKD-V) is associated with alterations in erythrocyte structure and functions which are the potential risk factors to interrupt erythrocyte proper activity however the mechanisms of such changes remain understudied. We used laser diffraction approach for investigation of CKD and hemodialysis (HD) effects on defor...
Article
Full-text available
Hypoxic conditions, in addition to effects on the CO2 and O2 transport, may have an impact on various functions of red blood cells (RBCs) including ion and metabolic regulation, oxidative stress induction, and transport of nitrites, ammonia, urea, etc. We used laser diffraction, spectrometry, and flow cytometry for evaluation of responses of RBCs o...
Article
Hypoxic conditions, in addition to effects on the CO 2 and O 2 transport, may have an impact on various functions of red blood cells (RBCs) including ion and metabolic regulation, oxidative stress induction , and transport of nitrites, ammonia, urea, etc. We used laser diffraction, spectrometry, and flow cytom-etry for evaluation of responses of RB...
Article
Ammonia is one of the main waste products of nitrogen metabolism that is transported from muscle and other peripheral tissues to the liver to be converted to urea in urea cycle in hepatocytes and excreted by kidneys in the form of urine. When nitrogen load exceeds the clearance capacity (“nitrogen imbalance”), the ammonia level exceeds 60 μM (hyper...
Chapter
Toxic and mechanistic properties of nutraceuticals are not as extensively studied as those of pharmaceuticals. Flow cytometry is among the most popular techniques to investigate the modes and mechanisms of cytotoxic action of chemical compounds, although flow cytometry methods are not suitable for online registration of fast changes of cell volumes...
Article
The ammonia/ammonium (NH3/NH4⁺) influx into red blood cells (RBCs) is mediated by surface glycoprotein RhAG that forms a structural complex with anion exchanger 1 (AE1, band 3). Owing to the activity of this complex, RBCs exposed to the isosmotic ammonium buffer swell and finally lyse. Isoosmotic NH4⁺-containing media alters the pH gradient in RBCs...
Article
Full-text available
Background Direct interaction between Red blood cells (RBCs) and platelets is known for a long time. The bleeding time is prolonged in anemic patients independent of their platelet count and could be corrected by transfusion of RBCs, which indicates that RBCs play an important role in hemostasis and platelet activation. However, in the last few yea...

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Projects

Project (1)
Project
Hyperammonemia is an important cause of cerebral edema in adults with liver failure, children with inborn errors of metabolism, in patients on hemodialysis, and even in adults under the strenuous physical exercises. In hyperammonemia the physiological level of ammonia in the bloodstream increases (>50mkM NH4+). Due to the chemical properties the uncharged ammonia molecule easily crosses the blood-brain barrier, thus increases the risk of hepatic encephalopathy, tremor, convulsions are observed and coma threat emerges in hyperammonemic state. Such condition obviously demands the fast medical correction, however the existing drugs lower the ammonia concentration in the bloodstream only slightly and time-consumingly. Overstressing ammonia/ammonium concentration has a bunch of targets and triggers various cytotoxic problems, which may lead to the cell death. Thus, in normal state the NH3/NH4+ level is tightly regulated in plasma and а the study of the mechanisms of such regulation becomes very important especially for the development and correction of the therapy for the patients with nitrogen metabolism abnormalities. Recently it was evaluated that erythroid RhAG glycoprotein facilitates ammonium transport, nevertheless the exact ligand as well as the transport mechanism are the questions of the debates yet. The investigations are complexified by the lack of the specific RhAG inhibitor. It was shown that ammonium transporters of the Rhesus family are expressed in the main sites (liver, kidneys, skin, gut), involved in generation, secretion and elimination of ammonia. The more interesting the fact of RhAG expression in red blood cells. Accordingly, the investigation of the physiological significance of the RhAG functions in red blood cells will let us estimate in greater detail the role of red blood cells in ammonia homeostasis. As a result of this project it’s intended to get the new original data on mechanisms of ammonium transport in human erythrocytes and the new data on the erythroid role in ammonium homeostasis. The obtained results will be the basis for the understanding of the nitrogen metabolism impairment regulation pathways and will lead to the formation of the new strategies of control and treatment in hyperammonemic patients. The investigation of the erythrocyte functional status in the following groups: (a) long term hemodialysis patients; (b) transient hyperammonemia of the newborns; (c) adults under the strenuous exercises, - will help us getting the new data on mechanisms that underlie these pathological conditions. Therefore, our data will elucidate the role of red blood cells in maintenance of the physiological NH3/NH4+ level in the bloodstream, and will help in understanding of the mechanism that are in charge of trapping and lodging of NH3/NH4+ by the red blood cells.