Julia Schückel

Julia Schückel
DKMS Life Science Lab GmbH · R&D

PhD

About

43
Publications
9,532
Reads
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831
Citations
Additional affiliations
September 2020 - present
DKMS Life Science Lab GmbH
Position
  • Senior Researcher
July 2017 - July 2020
GlycoSpot
GlycoSpot
Position
  • Project Manager
July 2016 - June 2017
GlycoSpot
GlycoSpot
Position
  • Senior Researcher
Education
October 2008 - February 2012
The University of York
Field of study
  • Biochemistry
October 2003 - August 2008
Technische Universität Dresden
Field of study
  • Chemistry
September 2001 - June 2003
Berufliches Schulzentrum Dippoldiswalde
Field of study
  • Chemistry and Biology

Publications

Publications (43)
Article
Background and aims: The necrotrophic fungus Botrytis cinerea infects a broad range of fruit crops including domesticated grapevine Vitis vinifera cultivars. Damages caused by this pathogen are severely detrimental to the table and wine grape industries and results in substantial crop losses worldwide. The apoplast and cell wall interface is an im...
Article
Full-text available
This study evaluates the capacity of four hydrolytic enzymes to limit the interactions between grape cell-walls and tannins and/or to favor tannin desorption. Adsorption and desorption tests were conducted by mixing a commercial seed tannin with purified skin cell-walls from Syrah grapes, in the presence or absence of hydrolytic enzymes, in a model...
Article
Phenolic composition of young red wines has been shown to play an important role in their ageing potential. Therefore, the modulation of phenolic extraction during maceration, may influence the subsequent phenolic evolution of these wines. The present work aimed to evaluate the impact of three different maceration times on the phenolic levels and e...
Article
To disentangle the role of polygalacturonase (PG) genes in strawberry softening, the two PG genes most expressed in ripe receptacle, FaPG1 and FaPG2, were down-regulated. Transgenic ripe fruits were firmer than wild type when PG genes were silenced individually. Simultaneous silencing of both PG genes by transgene stacking did not result in an addi...
Article
Full-text available
Cell cultures derived from strawberry fruit at different developmental stages have been obtained to evaluate their potential use to study different aspects of strawberry ripening. Callus from leaf and cortical tissue of unripe-green, white, and mature-red strawberry fruits were induced in a medium supplemented with 11.3 µM 2,4-dichlorophenoxyacetic...
Preprint
Tannins are present in grape skins and seeds from where they are transferred into the must-wine matrix during the maceration stages of winemaking. However, tannin transfer is often incomplete. This could be due, among other reasons, to tannins becoming bound to grape cell wall polysaccharides, including soluble polymers, which are released during v...
Article
Full-text available
Kluyveromyces marxianus Y885, an endopolygalacturonase (EPG) producing yeast, was evaluated for its capacity to hydrolyse the cell wall polymers of grape pomace as well as its general fermentation capabilities on this substrate. Small scale batch fermentations on autoclaved pomace inoculated with Y885 delivered 10 g/l ethanol and 7.7 g/l glycerol,...
Article
Full-text available
Because of their agricultural value, there is a great body of research dedicated to understanding the microorganisms responsible for rumen carbon degradation. However, we lack a holistic view of the microbial food web responsible for carbon processing in this ecosystem. Here, we sampled rumen-fistulated moose, allowing access to rumen microbial com...
Article
Full-text available
Since Saccharomyces cerevisiae strains display no to weak pectinase activity, the utilization of external pectinase is a common practice in winemaking to enhance the extraction of compounds located in the grape berry skins during maceration. In this study, the activity of the native endo-polygalacturonase of a Kluyveromyces marxianus strain, isolat...
Article
This study evaluated the relationship between cell wall breakdown, from Shiraz grapes harvested at three different ripeness levels and the colour and phenolics extracted during alcoholic fermentation into wines. Phenolic differences between the ripeness treatments were minimal after ¼ of the fermentation was completed. However, colour and phenolic...
Article
Phenolic compounds play an important role in colour stability and sensory properties of red wine. This study evaluated berry skin cell wall composition and how this influences grape and wine phenolics at different ripeness levels (21°Brix, 23°Brix, and 25°Brix) over two consecutive vintages. The vintage effect was highly significant, especially in...
Poster
Full-text available
Phenolic compounds are important quality indicators in red wines. These compounds are extracted during the alcoholic fermentation due to grape tissue breakdown and the degradation of the grape berry cell wall structure during maceration. However, there are lot of unknowns in the relationship between phenolic compounds and cell wall polysaccharides...
Chapter
In this chapter, we present a two-dimensional approach for high-throughput screening of endo-cellulases as well as other endo-acting enzymes. The method is based on chromogenic substrates, produced either from purified or complex material, providing valuable information about enzyme activity toward its target as well as that same target in a contex...
Article
Gute Qualität der Würze ist die Grundvoraussetzung für ein gutes Bier. Entscheidend dafür ist die Qualität der Rohstoffe. Dazu gehört auch die Enzymaktivität im Korn. Aktuelle Analysemethoden erfordern umfangreiche Vorbereitung, benötigen spezielles Laborequipment und Fachpersonal. Aber wäre es nicht revolutionär, wenn korneigene Enzyme direkt im M...
Article
Full-text available
The major nutrients available to human colonic Bacteroides species are glycans, exemplified by pectins, a network of covalently linked plant cell wall polysaccharides containing galacturonic acid (GalA). Metabolism of complex carbohydrates by the Bacteroides genus is orchestrated by polysaccharide utilization loci (PULs). In Bacteroides thetaiotaom...
Article
The major nutrients available to human colonic Bacteroides species are glycans, exemplified by pectins, a network of covalently linked plant cell wall polysaccharides containing galacturonic acid (GalA). Metabolism of complex carbohydrates by the Bacteroides genus is orchestrated by polysaccharide utilization loci (PULs). In Bacteroides thetaiotaom...
Article
Full-text available
Fungus-growing termites rely on the mutualistic fungus Termitomyces and gut microbes for plant biomass degradation. Due to a certain degree of symbiont complementarity, this tripartite symbiosis has evolved as a complex bioreactor, enabling decomposition of nearly any plant polymer, likely contributing to the success of the termites as the main pla...
Article
Full-text available
The adhesion of plant cells is vital for support and protection of the plant body and is maintained by a variety of molecular associations between cell wall components. In some specialized cases though, plant cells are programmed to detach and root cap-derived border cells are examples of this. Border cells (in some species known as border-like cel...
Article
Full-text available
A broad range of enzyme activities can be found in a wide range of different fruits and fruiting bodies but there is a lack of methods where many samples can be handled in a high-throughput and efficient manner. In particular, plant polysaccharide degrading enzymes-glycosyl hydrolases (GHs) play an important role in fruit development and ripening p...
Article
Full-text available
The selection of microbes by enrichment on plant biomass has been proposed as an efficient way to develop new strategies for lignocellulose saccharification. Here, we report an in-depth analysis of soil-derived microbial consortia that were trained to degrade once-used wheat straw (WS1-M), switchgrass (SG-M) and corn stover (CS-M) under aerobic and...
Article
Full-text available
Carbohydrates active enzymes (CAZymes) have multiple roles in vivo and are widely used for industrial processing in the biofuel, textile, detergent, paper and food industries. A deeper understanding of CAZymes is important from both fundamental biology and industrial standpoints. Vast numbers of CAZymes exist in nature (especially in microorganisms...
Article
Full-text available
The breakdown of plant cell wall (PCW) glycans is an important biological and industrial process. Noncatalytic carbohydrate binding modules (CBMs) fulfill a critical targeting function in PCW depolymerization. Defining the portfolio of CBMs, the CBMome, of a PCW degrading system is central to understanding the mechanisms by which microbes depolymer...
Article
Full-text available
Background Enzymes that degrade or modify polysaccharides are widespread in pro- and eukaryotes and have multiple biological roles and biotechnological applications. Recent advances in genome and secretome sequencing, together with associated bioinformatic tools, have enabled large numbers of carbohydrate-acting enzymes to be putatively identified....
Article
Full-text available
Carbohydrate-active enzymes have multiple biological roles and industrial applications. Advances in genome and transcriptome sequencing together with associated bioinformatics tools have identified vast numbers of putative carbohydrate-degrading and -modifying enzymes including glycoside hydrolases and lytic polysaccharide monooxygenases. However,...
Article
Full-text available
Recent metagenomic analyses have identified uncultured bacteria that are abundant in the rumen of herbivores and which possess putative biomass-converting enzyme systems. Here we investigate the saccharolytic capabilities of a Polysaccharide Utilization Locus (PUL) that has been reconstructed from an uncultured Bacteroidetes phylotype (SRM-1) that...
Article
Full-text available
Uncultured and therefore uncharacterized Bacteroidetes lineages are ubiquitous in many natural ecosystems which specialize in lignocellulose degradation. However, their metabolic contribution remains mysterious, as well-studied cultured Bacteroidetes have been shown to degrade only soluble polysaccharides within the human distal gut and herbivore r...
Article
Cytochromes P450 from plants have the potential to be valuable catalysts for industrial hydroxylation reactions, but their application is hindered by poor solubility, the lack of suitable expression systems and the requirement of P450s for auxiliary redox-transport proteins for the delivery of reducing equivalents from NAD(P)H. In the interests of...
Article
Full-text available
To date more than 15000 Cytochromes P450 have been identified and named so far, with one third belonging to the plant kingdom. This is a key biochemical resource, providing a wealth of biocatalysts covering a diverse range of chemistries. Characterisation, however, has been greatly hindered by the poor solubility of many P450s, a result of the memb...
Article
In the culture filtrate of a Marasmius sp. strain isolated in Indonesia during a screening for fungi with the ability to decolorize textile dyes, two laccase-related enzymes (laccase-related enzyme I and II) were detected. Laccase-related enzyme I was purified to homogeneity by ion exchange and hydrophobic interaction chromatography. The native enz...

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Projects

Projects (2)
Project
My main interest lies in method and assay development, finding new ways to elucidate and investigate different aspects of cellular, physiological and more generally, biological processes. I specialize in bio-organic chemistry and I have always striven to be on the interdisciplinary border of applied synthetic chemistry and different aspects of biology such as immunology, cell biology, biotechnology and many others. I find it fascinating and rewarding when I can synthesize a new synthetic compound that has the ability to reveal or elucidate an important biological process. Through the years and through collaboration with some excellent scientists, I have mainly focused on glycobiology and aspects of biology of different organisms that involve carbohydrates. By facing different biological problems, I have learned a lot about how to use synthetic chemistry to contribute to solving and elucidating biological processes. During my career, and with special focus on applied synthetic bioorganic chemistry, I have acquired significant experience in analytical, biochemical, immunological, microarray and automated data-processing techniques.
Project
In June 2011, the Marie Curie initial training network (ITN) WallTraC (the Plant Cell Wall Training Consortium), coordinated by Dr marie-Christine Ralet (Inra Angers-Nantes, France), brought together nine leading academic and private beneficiaries and two associated partners around two strategic objectives: • To develop new tools and concepts to advance knowledge on plant cell walls, • To provide high quality, inter-sectorial and trans-disciplinary training to young research fellows. The consortium has successfully trained 13 young researchers - 11 PhD students and 2 post-doctoral researchers - from all over the world. Together, they expanded knowledge in plant cell walls by exploring a wide range of research fields going from enzymology to food polymer processing, through biochemistry, immunolabelling, bioinformatics, textile processing and crystallography. Throughout the whole project, 50 articles were published in peer-reviewed journals.