Jürgen Rybak

Jürgen Rybak
Max Planck Institute for Chemical Ecology | ice · Department of Evolutionary Neuroethology

Dr. rer. nat.

About

101
Publications
22,799
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2,825
Citations
Citations since 2017
33 Research Items
1068 Citations
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2017201820192020202120222023050100150200
2017201820192020202120222023050100150200
Introduction
Jürgen Rybak works at the Department of Evolutionary Neuroethology, Max Planck Institute for Chemical Ecology. Jürgen does research in Neuroscience and Evolutionary Biology..
Additional affiliations
July 2009 - May 2022
Max Planck Institute for Chemical Ecology
Position
  • Group Leader
January 2009 - June 2009
Universität Konstanz
Position
  • Lecturer
Description
  • Brain Evolution
January 2004 - December 2008
Freie Universität Berlin
Position
  • Research Associate

Publications

Publications (101)
Chapter
Full-text available
For a comprehensive understanding of brain function, compiling data from a range of experiments is necessary. Digital brain atlases provide useful refer- ence systems at the interface of neuroanatomy, neurophysiology, behavioral biology and neuroinformatics. Insect brains are particularly useful because they constitute complete three-dimensional re...
Chapter
A fundamental objective in neurobiology is to understand the neuronal circuitry that underlies different aspects of behavior (sensory integration, decision making, motor control, learning, and memory formation). In invertebrates, neural circuitry is classically analyzed at the cellular level using sparse reconstruction based on single cell staining...
Article
Full-text available
Detecting danger is one of the foremost tasks for a neural system. Larval parasitoids constitute clear danger to Drosophila, as up to 80% of fly larvae become parasitized in nature. We show that Drosophila melanogaster larvae and adults avoid sites smelling of the main parasitoid enemies, Leptopilina wasps. This avoidance is mediated via a highly s...
Article
Full-text available
In Drosophila melanogaster olfactory sensory neurons (OSNs) establish synapses with projection neurons (PNs) and local interneurons within antennal lobe (AL) glomeruli. Substantial knowledge regarding this circuitry has been obtained by functional studies, while ultrastructural evidence of synaptic contacts is scarce. To fill this gap we studied se...
Chapter
In the vinegar fly (Drosophila melanogaster), the neuronal pathway that processes olfactory information is organized into multiple layers: a peripheral set of olfactory sensory neurons (OSNs); the primary olfactory center, or antennal lobe (AL); and two second-order neuropils, the mushroom body (MB) and lateral horn (LH). Odorants are detected by t...
Preprint
Full-text available
To manage the great complexity of detecting and identifying olfactory cues, the insect olfactory system has evolved two main strategies: combinatorial coding and specialized, narrowly tuned olfactory pathways. In combinatorial coding, odorants are encoded by activation of multiple, broadly tuned olfactory sensory neurons that innervate distinct set...
Article
Full-text available
Insect neuroscience generates vast amounts of highly diverse data, of which only a small fraction are findable, accessible and reusable. To promote an open data culture, we have therefore developed the InsectBrainDatabase ( IBdb ), a free online platform for insect neuroanatomical and functional data. The IBdb facilitates biological insight by enab...
Article
Full-text available
Changes in behaviour often drive rapid adaptive evolution and speciation. However, the mechanistic basis for behavioural shifts is largely unknown. The tephritid fruit fly Rhagoletis pomonella is an example of ecological specialization and speciation in action via a recent host plant shift from hawthorn to apple. These flies primarily use specific...
Preprint
Full-text available
Insect neuroscience generates vast amounts of highly diverse data, of which only a small fraction are findable, accessible and reusable, despite open data mandates by funding bodies. We have therefore developed the InsectBrainDatabase (IBdb), an open platform for depositing, sharing and managing a wide range of insect neuroanatomical and functional...
Article
Full-text available
The hawkmoth Manduca sexta and one of its preferred hosts in the North American Southwest, Datura wrightii , share a model insect–plant relationship based on mutualistic and antagonistic life-history traits. D. wrightii is the innately preferred nectar source and oviposition host for M. sexta . Hence, the hawkmoth is an important pollinator while t...
Article
Full-text available
Divergent populations across different environments are exposed to critical sensory information related to locating a host or mate, as well as avoiding predators and pathogens. These sensory signals generate evolutionary changes in neuroanatomy and behavior; however, few studies have investigated patterns of neural architecture that occur between s...
Data
Supplementary Figures and Figure Legends "Inverse resource allocation between vision and olfaction across the genus Drosophila" (Keesey et al. 2019 NCOMMS)
Data
Reviewers comments (and author responses) across all 3 revision stages (Keesey et al. 2019 NCOMMS)
Data
Description of Additional Supplementary Files (Keesey et al. 2019 NCOMMS)
Data
Accession numbers for molecular phylogeny (Excel table) (Keesey et al. 2019 NCOMMS)
Data
Reporting Summary (Keesey et al. 2019 NCOMMS)
Preprint
Full-text available
Plant volatile detection through olfaction plays a crucial role in insect behaviors. In vivo , the odorant receptor co-receptor orco is an obligatory component for the function of odorant receptors (ORs), a major receptor family involved in insect olfaction. We used CRISPR-Cas9 targeted mutagenesis to knock-out (KO) orco in a neurophysiological mod...
Article
Full-text available
Here we report on ultrastructural features of brain synapses in the fly Drosophila melanogaster and outline a perspective for the study of their functional significance. Images taken with the aid of focused ion beam-scanning electron microscopy (EM) at 20 nm intervals across olfactory glomerulus DA2 revealed that some synaptic boutons are penetrate...
Chapter
The antennal lobe (AL) of an insect is the functional analog of the olfactory bulb in mammals. The first-level synaptic interaction of large numbers of multiple types of olfactory receptor neurons (OSNs) with AL interneurons serves the function of reliably coding a vast range of odorants and their mixtures and the separation between odor identity a...
Chapter
The mushroom body (MB) in the insect brain is composed of a large number of densely packed neurons called Kenyon cells (KCs) (Drosophila, 2200; honeybee, 170,000). In most insect species, the MB consists of two caplike dorsal structures, the calyces, which contain the dendrites of KCs, and two to four lobes formed by collaterals of branching KC axo...
Article
Full-text available
Background: Odor information is processed through multiple receptor-glomerular channels in the first order olfactory center, the antennal lobe (AL), then reformatted into higher brain centers and eventually perceived by the fly. To reveal the logic of olfaction, it is fundamental to map odor representations from the glomerular channels into higher...
Article
Full-text available
Background Odor information is processed through multiple receptor-glomerular channels in the first order olfactory center, the antennal lobe (AL), then reformatted into higher brain centers and eventually perceived by the fly. To reveal the logic of olfaction, it is fundamental to map odor representations from the glomerular channels into higher b...
Article
Full-text available
A characterization of the bacterial community of the hindgut wall of two larval and the adult stages of the forest cockchafer (Melolontha hippocastani) was carried out using amplicon sequencing of the 16S rRNA gene fragment. We found that, in second-instar larvae, Caulobacteraceae and Pseudomonadaceae showed the highest relative abundances, while i...
Article
Full-text available
In the honeybee brain, two prominent tracts - the medial and the lateral antennal lobe tract 17 - project from the primary olfactory center, the antennal lobes, to the central brain, the 18 mushroom bodies, and the protocerebral lobe. Intracellularly stained uniglomerular 19 projection neurons (uPN) were reconstructed, registered to the 3D honeybee...
Chapter
The study of neuronal circuits that mediate an animal's behavior requires a multidisciplinary approach. The brains of arthropods, such as the fruit fly Drosophila melanogaster and the honey bee Apis mellifera, can be investigated at the level of single cells or small networks. These important model systems allow us to unravel neural correlates and...
Data
Raw data the figures of this manuscript are based on. (XLSX)
Data
Iridoid compounds produced by L. boulardi and L. heterotoma. Total ion current (TIC) chromatograms on a nonpolar (BPX5) GC column of an extract of females of (A) L. boulardi and (B) L. heterotoma. (C) Molecular structure of the iridoid compounds found in L. boulardi and L. heterotoma. Numbers correspond to the peaks in (A) and (B). (D–G) Identifica...
Data
Diagnostic set of odors used to identify OSNs during SSRs. OSNs that are expected to exhibit strong responses to a specific odor are given in brackets. (TIF)
Data
Electrophysiological recordings with Drosophila OSNs and wasp odours. (A–D) SSR responses of wildtype ab10B neurons tested with the headspace (A) or wash (B) of L. boulardi, or synthetic (-)-iridomyrmecin (C), (R)-actinidine (D), or nepetelactol (a mixture of 1S4aR7R7aS-Nepetalactol, 1R4aS7S7aS-Nepetalactol and their enantiomers) (E). (F) Dorsal-o...
Data
Odor panel used to screen Or49a and Or85f in the empty neuron system, color-coded by functional group (red, alcohols; blue, esters; gray, acids; brown, ketones; pink, aldehydes; light green, nitrogen-containing compounds; purple, terpenes; dark green, alkanes; black, other compounds). (TIF)
Data
Localization of OSNs expressing Or49a on the antenna of a female D. melanogaster. OSNs are visualized by expressing GCaMP3.0 under control of Gal4-Or49a driver line. (TIF)
Data
Response profiles of neurons paired with iridomyrmecin-, actinidine-, and nepetalactol -responsive neurons shown in Fig 4C (n = 3). Error bars represent standard error of the mean (SEM). (TIF)
Data
GC-SSR responses of a neuron misexpressing Or49a to different isomers of iridomyrmecin. Top line named with the compound depicts the flame ionization detector (FID) signal of the GC. (TIF)
Data
GC-SSR responses of a neuron misexpressing Or85f to different isomers of actinidine and nepetalactol. Top line named with the compound depicts the FID signal of the GC. (TIF)
Data
Behavioral avoidance of synthetic compounds. Larval choice assay and oviposition assay and resulting preference indices when exposed to the synthetic (-)-iridomyrmecin and (R)-actinidine. Deviation of the indices against zero was tested with Wilcoxon rank sum test. Asterisks, p < 0.05; error bars depict standard deviation. PI = (number of larvae, f...
Article
Full-text available
The Coenobitidae (Decapoda, Anomura, Paguroidea) is a taxon of hermit crabs that includes two genera with a fully terrestrial life style as adults. Previous studies have shown that Coenobitidae have evolved a sense of spatial odor localization that is behaviorally highly relevant. Here, we examined the central olfactory pathway of these animals by...
Article
Full-text available
To internally reflect the sensory environment, animals create neural maps encoding the external stimulus space. From that primary neural code relevant information has to be extracted for accurate navigation. We analyzed how different odor features such as hedonic valence and intensity are functionally integrated in the lateral horn (LH) of the vine...
Article
Full-text available
Intact function of the Forkhead Box P2 (FOXP2) gene is necessary for normal development of speech and language. This important role has recently been extended, first to other forms of vocal learning in animals and then also to other forms of motor learning. The homology in structure and in function among the FoxP gene members raises the possibility...
Article
Full-text available
We compared the morphology of the primary olfactory center, the antennal lobe (AL), in 2 homopteran insects, Hyalesthes obsoletus Signoret (Homoptera: Cixiidae) and Scaphoideus titanus Ball (Homoptera: Cicadomorpha). The comparison between the ALs of the 2 species is particularly interesting considering that, although both use volatile cues to loca...
Poster
Full-text available
Anetennal Lobe Drosophila melanogaster
Poster
Full-text available
Anetennal lobe Drosophila melanogaster
Conference Paper
Full-text available
Background / Purpose: In humans, mutations of the transcription factor Forkhead box protein P2 (FOXP2) cause a severe speech and language disorder. Down regulating the Zebrafinch FOXP2 orthologue in development, results in incomplete and inaccurate song imitation. Because both language and song learning can be seen as instances of operant trial-a...
Chapter
The honeybee mushroom bodies (MBs) are high order central brain neuropils that communicate with all sensory modalities via inputs at the calyces and mixed inputs and outputs along their two lobes. The dendrites of the intrinsic neurons (Kenyon cells) are either widefield (type KI) or narrow-banded (type KII), and participate in a microcircuit of th...
Article
Full-text available
The Drosophila antennal lobe (AL) has become an excellent model for studying early olfactory processing mechanisms. Local interneurons (LNs) connect a large number of glomeruli and are ideally positioned to increase computational capabilities of odor information processing in the AL. Although the neural circuit of the Drosophila AL has been intensi...
Data
Full-text available
Source: Supplemental Data: 3D-The-Digital-Bee-Brain.: Rybak et al., 2010,Front. Syst. Neurosci., 25 July 2010 | doi: 10.3389/fnsys.2010.00030. Download PDF, open in Acrobat Reader ,drag right-mouse mouse over 'interactive view' in Figure 2A, allows you using the register of the modelTree view all object in 3D view
Article
Full-text available
The honeybee standard brain (HSB) serves as an interactive tool for relating morphologies of bee brain neurons and provides a reference system for functional and bibliographical properties (http://www.neurobiologie.fu-berlin.de/beebrain/). The ultimate goal is to document not only the morphological network properties of neurons collected from separ...
Article
Full-text available
Many bioactive neuropeptides containing RFamide at their C terminus have been described in both invertebrates and vertebrates. To obtain insight into the functional logic of RFamide signaling, we investigate it here in the feeding system of Aplysia. We focus on the expression, localization, and actions of two families of RFamide peptides, the FRFam...
Article
Full-text available
We use the moth Heliothis virescens as model organism for studying the neural network involved in chemosensory coding and learning. The constituent neurons are characterised by intracellular recordings combined with staining, resulting in a single neuron identified in each brain preparation. In order to spatially relate the neurons of different pre...
Conference Paper
Full-text available
The HoneyBee Standard Brain (HSB) serves as an interactive tool for comparing morphologies of bee brain neurons and relates it to functional as well as biblographical properties [1]. Recent efforts by several labs have accumulated confocal image stacks from extra- and intracellular stained neurons in the bee central nervous system [2]. We present...
Article
Honeybees detect airborne vibration by means of Johnston's organ (JO), located in the pedicel of each antenna. In this study we identified two types of vibration-sensitive interneurons with arborizations in the primary sensory area of the JO, namely, the dorsal lobe-interneuron 1 (DL-Int-1) and dorsal lobe-interneuron 2 (DL-Int-2) using intracellul...