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Publications (181)
Cool-season grain legumes are mostly grown over spring and summer due to poor frost tolerance. However, fall-sown varieties often provide higher yields, earlier harvests and avoid late-season drought and heat. Understanding the genetic determinism and molecular basis of frost tolerance is therefore crucial for developing high-performing winter vari...
The taxonomy of the tribe Fabeae (Vicieae) has long been problematic, but an analysis by Schaefer et al. in 2012 gave an exceptionally clear view of the tribe and noted the possibility that some nomenclatural adjustments may be required at some future date. These authors suggested several options, expressing some preferences. However, there has bee...
The taxonomy of the Fabeae (Vicieae) has long been problematic, but an analysis by Schaefer et al in 2012 gave an exceptionally clear view of the tribe and noted the possibility that some nomenclatural adjustments may be required at some future date. These authors suggested several options, expressing some preferences. However, there has been a rec...
The afila (af) mutation causes the replacement of leaflets by a branched mass of tendrils in the compound leaves of pea – Pisum sativum L. This mutation was first described in 1953, and several reports of spontaneous af mutations and induced mutants with a similar phenotype exist. Despite widespread introgression into breeding material, the nature...
Improving the seed protein concentration (SPC) of pea (Pisum sativum L.) has turned into an important breeding objective because of the consumer demand for plant-based protein and demand from protein fractionation industries. To support the marker-assisted selection (MAS) of SPC towards accelerated breeding of improved cultivars, we have explored t...
Genomic resources are becoming available for Pisum but to link these to phenotypic diversity requires well marked populations segregating for relevant traits. Here we describe two such resources. Two recombinant inbred populations, derived from wide crosses in Pisum are described. One high resolution mapping population involves cv Caméor, for which...
While the continuing decline in genotyping and sequencing costs has largely benefited plant research, some key species for meeting the challenges of agriculture remain mostly understudied. As a result, heterogeneous datasets for different traits are available for a significant number of these species. As gene structures and functions are to some ex...
The afila ( af ) mutation of Pisum sativum L. (pea) is characterised by leaves that are composed of a basal pair of stipules, a petiole and a branched mass of tendrils. These are bipinnate leaves in which the leaflet primordia are replaced by midrib-like, or terminal tendril, primordia. The phenotype was first reported as a spontaneous mutation in...
Centromeres in the legume genera Pisum and Lathyrus exhibit unique morphological characteristics, including extended primary constrictions and multiple separate domains of centromeric chromatin. These so-called metapolycentromeres resemble an intermediate form between monocentric and holocentric types, and therefore provide a great opportunity for...
Centromeres in the legume genera Pisum and Lathyrus exhibit unique morphological characteristics, including extended primary constrictions and multiple separate domains of centromeric chromatin. These so-called metapolycentromeres resemble an intermediate form between monocentric and holocentric types, and therefore provide a great opportunity for...
Frost is a major abiotic stress of winter type faba beans (Vica faba L.) and has adverse effects on crop yield. Climate change, far from reducing the incidence of frost events, is making these phenomena more and more common, severe, and prolonged. Despite the important interaction that the environment has in the tolerance of faba bean to frost, thi...
Background & Summary
In addition to being the model plant used by Mendel ¹ to establish genetic laws, pea ( Pisum sativum L.) is a major pulse crop cultivated in many temperate regions of the world. In order to face new challenges imposed particularly by global climate change and new regulations targeted at reducing chemical inputs, pea breeders ha...
Key message
A genome-wide association study for pea resistance against a pea-adapted biotype and a non-adapted biotype of the aphid, Acyrthosiphon pisum, identified a genomic region conferring resistance to both biotypes.
Abstract
In a context of reduced insecticide use, the development of cultivars resistant to insect pests is crucial for an inte...
Rhizobium-legume nitrogen-fixing symbiosis involves the formation of a specific organ, the root nodule, which provides bacteria with the proper cellular environment for atmospheric nitrogen fixation. Coordinated differentiation of plant and bacterial cells is an essential step of nodule development, for which few transcriptional regulators have bee...
The timing of developmental phase transitions is crucial for plant reproductive success, and two microRNAs (miRNA), miR156 and miR172, are implicated in the control of these changes, together with their respective SQUAMOSA promoter binding–like (SPL) and APETALA2-like (AP2) targets. While their patterns of regulation have been studied in a growing...
Agroecological principles are to value the ecosystemic services delivered by enlarged planned and natural biodiversity to sustain regulation and support services for agricultural production. Maximizing the biological functions provided by biodiversity requires to profoundly re-design agricultural systems, considering the management of both cropping...
The timing of developmental phase transitions is crucial for plant reproductive success, and two microRNAs (miRNA), miR156 and miR172, are implicated in the control of these changes, together with their respective SQUAMOSA promoter binding like (SPL) and APETALA2 (AP2) like targets. While their patterns of regulation have been studied in a growing...
The French government has supported as part of its “Investments for the Future” program a 9-year research project, PeaMUST, devoted to pea and to a lesser extent, faba bean improvement. Focusing on the main causes of yield irregularity that limit pea and faba bean cultivation, an integrated approach, including molecular exploitation of the pea geno...
The French government has supported as part of its“Investments for the Future” program a 9-year research project, PeaMUST, devoted to pea and to a lesser extent, faba bean improvement. Focusing on the main causes of yield irregularity that limit pea and faba bean cultivation, an integrated approach, including molecular exploitation of the pea genom...
Plant-plant associations, notably cereal-legume intercropping, have been proposed in agroecology to better value resources and thus reduce the use of chemical inputs in agriculture. Wheat-pea intercropping allows to decreasing the use of nitrogen fertilization through ecological processes such as niche complementarity and facilitation. Rhizosphere...
Pea is one of the most important grain legume crops in temperate regions worldwide. Improving pea yield is a critical breeding target. Nine inter-connected pea recombinant inbred line populations were evaluated in nine environments at INRAE Dijon, France and genotyped using the GenoPea 13.2 K SNP array. Each population has been evaluated in two to...
Le développement des légumineuses dans la sole française, facteur dedurabilité, doit faire face à des contraintes de production qui peuventpénaliser leur rentabilité. Des avancées significatives, obtenues en poisd’hiver, devraient accélérer le progrès génétique et favoriser cette culture.
Background:
Frost is a limiting abiotic stress for the winter pea crop (Pisum sativum L.) and identifying the genetic determinants of frost tolerance is a major issue to breed varieties for cold northern areas. Quantitative trait loci (QTLs) have previously been detected from bi-parental mapping populations, giving an overview of the genome region...
Faba bean (Vicia faba L.) is a pulse crop of high nutritional value and high importance for sustainable agriculture and soil protection. With the objective of identifying gene-based SNPs, transcriptome sequencing was performed in order to reduce faba bean genome complexity. A set of 1,819 gene-based Snp markers polymorphic in three recombinant line...
Faba bean (Vicia faba L.) is a pulse crop of high nutritional value and high importance for sustainable agriculture and soil protection. With the objective of identifying gene-based SNPs, transcriptome sequencing was performed in order to reduce faba bean genome complexity. A set of 1,819 gene-based SNP markers polymorphic in three recombinant line...
Genome-wide association study (GWAS) was conducted to identify loci associated with agronomic (days to flowering, days to maturity, plant height, seed yield and seed weight), seed morphology (shape and dimpling), and seed quality (protein, starch, and fiber concentrations) traits of field pea (Pisum sativum L.). A collection of 135 pea accessions f...
We report the first annotated chromosome-level reference genome assembly for pea, Gregor Mendel’s original genetic model. Phylogenetics and paleogenomics show genomic rearrangements across legumes and suggest a major role for repetitive elements in pea genome evolution. Compared to other sequenced Leguminosae genomes, the pea genome shows intense g...
Pea (Pisum sativum L.) is an important source of dietary proteins. Nutrient recycling from leaves contributes to the accumulation of seed proteins and is a pivotal determinant of protein yields in this grain legume. The aim of this study was to unveil the transcriptional regulations occurring in pea leaves before the sharp decrease in chlorophyll b...
The recently generated pea genome sequence is a significant step for the pea research community towards unravelling functional diversity and establishing genome-enabled breeding. Re-sequencing data reveal the considerable diversity present in the Pisum genus. High-throughput genotyping is now available to explore large collections using the exome c...
Whole genome profiling (WGP) is a sequence-based physical mapping technology and uses sequence tags generated by next generation sequencing for construction of bacterial artificial chromosome (BAC) contigs of complex genomes. The physical map provides a framework for assembly of genome sequence and information for localization of genes that are dif...
Unique sequence tags identified by sequencing ends of restriction fragments of 295,680 BAC clones.
Number of BAC clones in each contig built based on the sequence similarities of unique sequence tags.
Distribution of BAC clones in contigs built based on the sequence similarities of unique sequence tags.
Fingerprinted Contig output file to visualize all the BAC contigs and overlap of each BAC in the reported contigs.
BAC clones corresponding to the unique sequence tags identified by sequencing ends of restriction fragments of 295,680 BAC clones.
Published in Grain Legumes, 52, 16-17, 2009
Whole genome profiling (WGP) is a sequence-based physical mapping technology and uses sequence tags generated by next generation sequencing for construction of bacterial artificial chromosome (BAC) contigs of complex genomes. The physical map provides a framework for assembly of genome sequence and information for localization of genes that are dif...
Pea is the most widely cultivated grain legume crop in Europe. In the French research project PeaMUST, a large public and private sector partnership has been set up to undertake complementary strategies towards the development of high and stable yielding cultivars. These different strategies will contribute to the definition of a pea ideotype based...
The French National Institute for Agricultural Research (INRA) has established an ambitious, multi-scale, agricultural experimental infrastructure (the CA-SYS platform). CA-SYS covers an area of 120 ha and will be initiated in autumn 2018. The aims of CA-SYS are to: i) design and evaluate new agroecological systems; ii) study the transition from cu...
Pea forms symbiotic nodules with Rhizobium leguminosarum sv. viciae (Rlv). In the field, pea roots can be exposed to multiple compatible Rlv strains. Little is known about the mechanisms underlying the competitiveness for nodulation of Rlv strains and the ability of pea to choose between diverse compatible Rlv strains. The variability of pea-Rlv pa...
Combining plant genetic resistance with architectural traits that are unfavorable to disease development is a promising strategy for reducing epidemics. However, few studies have identified root system architecture (RSA) traits with the potential to limit root disease development. Pea is a major cultivated legume worldwide and has a wide level of n...
Frequency distribution of least square means obtained for root, shoot and overall plant architecture variables, and for Aphanomyces root rot resistance in the 266-pea-line collection. Least square means were obtained from analysis of variance for six root system architecture variables (total projected area, average root diameter, total root length,...
Clustered heatmap of the Kinship matrix of the 266-pea-line collection.Kinship matrix from the GAPIT R package (Lipka et al., 2012) based on 2,937 SNP markers. Clustering is based on the UPGMA method. The color gradient represents the degree of relationship between two lines shown. Kinship matrix from the GAPIT R package (Lipka et al., 2012) based...
Linkage disequilibrium (LD) decay in the 266-pea-line collection. Colored curves represent the estimated LD decay for each linkage group (LG). Dashed vertical lines represent the LD threshold (maximum r2/2) and arrows the LD decay rate, as the estimated genetic distance (cM) to reach this LD threshold on each LG.
Regression line between disease severity score and percentage of brown projected area measured in Exp#3. LSMeans of disease severity (DS) score was plotted against LSMeans of percentage of brown projected area (Br:TProjArea). Function of the regression line (y = 0.25804x + 0.06497) was calculated through the linear model Br:TProjArea DS. The regres...
Comparative genetic map of genome-wide association (GWA) and previously detected linkage quantitative trait loci (QTL) for resistance to A. euteiches and plant architecture on LGI to VI. The comparative genetic map was constructed from the projection of the 953 markers from Boutet et al. (2016) onto the consensus THMap from Desgroux et al. (2016)....
Statistical analysis results of plant architecture and Aphanomyces root rot resistance data in the 266-pea-line collection.
Genetic markers associated with plant architecture and Aphanomyces root rot resistance variables detected by genome-wide association mapping.
Pea (Pisum sativum, L.) is a major pulse crop used both for animal and human alimentation. Owing to its association with nitrogen-fixing bacteria, it is also a valuable component for low-input cropping systems. To evaluate the genetic diversity and the scale of linkage disequilibrium (LD) decay in pea, we genotyped a collection of 917 accessions ga...
