Juana Frias

Spanish National Research Council | CSIC · Institute of Food Science, Technology and Nutrition

The impact of different pressure levels in the HHP-assisted hydrolysis by Alcalase of quinoa proteins on the catalytic efficiency, peptide release, phenolic compounds content, and biological activities was investigated. The protein profile (SDS-PAGE) showed a more extensive peptide breakdown for the HHP-assisted proteolysis at 300–400 MPa, which wa...

The impact of different pressure levels in the HHP-assisted hydrolysis by Alcalase of quinoa proteins on the catalytic efficiency, peptide release, phenolic compounds content, and biological activities was investigated. The protein profile (SDS-PAGE) showed a more extensive peptide breakdown for the HHP-assisted proteolysis at 300–400 MPa, which wa...

Germination time is a critical factor that influences the digestibility and bioactivity of proteins in pulses. The objective was to understand the effect of sprouting time on protein hydrolysis (PH) and the release of bioactive peptides during digestion of lupin (Lupinus angustifolius L.) to provide recommendations on the optimum germination time f...

Previous studies demonstrated that enzymatic hydrolysis enhances wheat bran (WB) biological properties. This study evaluated the immunostimulatory effect of a WB hydrolysate (HYD) and a mousse enriched with HYD (MH) before and after in vitro digestion on murine and human macrophages. The antiproliferative activity of the harvested macrophage supern...

Objeto: la crisis de la COVID-19 ha causado un cambio profundo en el estilo de vida de la ciudadanía. Las olas previas han demostrado que en régimen de confinamiento se pueden desarrollar problemas de salud mental y hay efectos sobre la actividad física y cambios en los hábitos dietéticos. Tema y metodología: se envió a los trabajadores del Consejo...

In this study, the comprehensive chemical characterization of red lentil hulls obtained from the industrial production of football and split lentils was described. The lentil hulls were rich in dietary fiber (78.43 g/100 g dry weight with an insoluble to soluble fiber ratio of 4:1) and polyphenols (49.3 mg GAE/g dry weight, of which 55% was bound p...

The tailored formulation of raw materials and the combination of grain germination and extrusion processes could be a promising strategy to achieve the desired goal of developing healthier expanded extrudates without compromising sensory properties. In this study, modifications in the nutritional, bioactive profile and physicochemical properties of...

Bioactive compounds, such as phenolic compounds, are phytochemicals found in significant amounts in cereals and pseudocereals and are usually evaluated by spectrophotometric (UV-VIS), HPLC, and LC-MS techniques. However, their bioavailability in grains is quite limited. This restriction on bioavailability and bioaccessibility occurs because they ar...

To enlarge the applications of whole wheat grain (WWG) and wheat bran (WB) as functional ingredients in foodstuffs that can promote human health, researchers have explored bioprocessing approaches to improve the bioaccessibility of phenolic compounds from these food matrices and, subsequently, their biological effects. The objective of this study w...

Germination is an efficient and natural strategy that allows the modification of the nutritional value and the nutraceutical properties of seeds, enabling one to tailor the process according to its final use. This study aimed at optimization of germination conditions to produce novel lentil flours with improved nutritional and functional features....

The present study focuses on the utilization of moringa sprout powder (MSP) for development of healthy and ready-to-eat snack bars. Different MSP proportions (9–26%) were evaluated for snack bar formulation and compared with control formulation (0% MSP). 18% MSP-containing prototype (18MSP) was selected as the optimal formulation based on the resul...

Germination offers advantages to improve legume protein digestibility as it disintegrates seed structure and hydrolyzes proteins and anti-nutrients. Seed permeability (related to polyphenol content of seed coats) is an important factor affecting the duration of seed germination and its impact on protein digestibility and bioactivity. The objective...

The safety and health effects for celiac people of a novel beverage (SOFB) developed from sprouted oat flour by fermentation with Lactobacillus plantarum was explored. In vitro reactivity against anti-gliadin antibodies (AGA) and antioxidant/anti-inflammatory potential of SOFB in RAW 264.7 macrophages and Caco-2 cells were evaluated. Immunoreactivi...

Wheat bran (WB) is a byproduct from the milling industry that contains bioactive compounds beneficial to human health. The aim of this work was on the one hand, increasing extracta-bility of antioxidant and anti-inflammatory compounds (specifically ferulic acid, FA), through en-zymatic hydrolysis combined with hydrothermal treatment (HT) and high h...

Five fettuccini formulations containing 5% (5MSP), 10% (10MSP), 15% (15MSP), 20% (20MSP), and 30% (30MSP) of moringa sprout powder (MSP) were produced aimed at improving the nutritional and bioactive profile of conventional pasta. A gradual increase of protein, lipids, fiber and mineral content was observed in fettuccine as the MSP amount increased...

This study investigates the use of sprouted oat flour as a substrate to develop a novel gluten-free beverage by fermentation with a probiotic (Lactobacillus plantarum WCFS1) starter culture. Physicochemical, microbiological, nutritional and sensory properties of sprouted oat fermented beverage (SOFB) were characterized. After fermentation for 4 h,...

Bran, a byproduct still mainly used for animal feed, is receiving increased attention as potential ingredient for a healthier diet. The aim of this study was to characterize and evaluate the nutritional and antioxidant properties of wheat and oat bran in order to promote their use as nutraceutical ingredients in flour and/or other products. The eff...

Moringa oleifera L. is greatly appreciated for its high content of phytochemicals. Although most parts of moringa tree have been widely studied, seeds remained scarcely explored. The first goal of this study was to investigate the effectiveness of germination to improve the nutritional composition (proximate composition and levels of vitamins B1 an...

Legumes are alternative protein sources that have been successfully used to develop diverse meatless foods. Although these plant-based products have a lower impact on the environment than equivalent animal-based products, they have lower protein digestibility. Germination could be a useful bioprocess to enhance protein digestibility in legumes, alt...

The world population grows with the tendency to concentrate in urban areas. Having food for everyone and correct information on nutrients and diet for everyone is included in the global scope of the United Nations Millennium Sustainable Development Goals (SDGs). Science and technology play a key role. Recently the term ‘Ultra-processed’ has become...

Valorization of wheat bran (WB) into new high-value products is of great interest within the framework of sustainability and circular economy. In the present study, we utilized a multi-step approach to extract nutraceutical compounds (phenolic acids) from WB and improved its antioxidant and anti-inflammatory properties through using sequential hydr...

This study is aimed to produce and characterize a novel gluten-free ingredient from oat through sprouting at 18 °C for 96 h. The nutritional and bioactive properties as well as key enzymatic activities were studied in sprouted oat powder and compared with those of oat grain powder (control). Sprouted oat powder was an excellent source of protein (1...

There is a growing interest in the development of flours from sprouted cereals due to their enhanced nutritional and bioactive potential, but this research field has been scarcely explored. This study aimed to investigate the influence of germination conditions on protein profile, bioactive potential and enzymatic activities related with quality in...

The study evaluated the effect of autoclaving as a hydrothermal treatment on the quality and bioactivity of wheat bran (WB) with the objective of producing a natural ingredient with enhanced healthy properties. Nutritional, antioxidant, techno-functional and sensorial parameters were studied, and temperatures of 100, 115 and 130 °C were explored. O...

The polyphenolic content of dehydrated maca (MA) and mashua (M) powders, promising functional ingredients, and their antioxidant activity and inhibition of carbohydrate digestion was evaluated following in vitro gastrointestinal digestion (GID). Moreover, the impact of steaming on bioaccessibility and bioactivity of the M phenolic fraction was inve...

Knowledge on the specific variation in the phenolic composition of wheat defined by germination conditions and its relationship with antioxidant and anti-inflammatory properties of sprouts would be useful to improve the functional value of wheat-derived products. Variation in soluble phenolic composition, antioxidant and anti-inflammatory potential...

The increasing demand for healthy food products has promoted the use of germinated seeds to produce functional flours. In this study, germination conditions were optimized in barley grains with the aim to produce flours with high nutritional and biofunctional potential using response surface methodology (RSM). The impact of germination time (0.8–6...

Grain legumes are widely recognized as staple sources of dietary protein worldwide. Lentil seeds are an excellent source of plant-based proteins and represent a viable alternative to animal and soybean proteins for food processing formulations. Lentil proteins provide not only dietary amino acids but are also a source of bioactive peptides that pro...

The aim of this study was to determine the impact of three different technological processes and in vitro digestion on the profile and content of betalains and inhibition of angiotensin I converting enzyme (ACE) in red beet. Betalains were analysed by the micro-HPLC-TOF-MS/MS method, while ACE inhibition was determined by an in vitro assay. In the...

Outline LWT Volume 99, January 2019, Pages 306-312 Development of a multifunctional yogurt-like product from germinated brown rice Author links open overlay panelPatricio J.CáceresaJuanaFríasb Show more https://doi.or The suitability of germinated brown rice (GBR) for developing novel multifunctional yogurt-like products was evaluated. Crude brown...

This work evaluated the feasibility of pH-controlled fermentation by Lactobacillus plantarum CECT 748 combined with Savinase-hydrolysis (LPHS) for producing multifunctional lentil ingredients targeted for metabolic syndrome (MetS) relieving. LPHS process was compared with L. plantarum-fermentation (LP) and Savinase-hydrolysis (HS), applied separate...

Fermented lentil (FL) produced using L. plantarum and Savinase in alkaline conditions relieves metabolic alterations and oxidative stress in Zucker rats with metabolic syndrome. Here, we investigated the effect of up scaling the fermentation process on chemical composition and biological activity of FL. Moreover, we studied the molecular mechanisms...

The impact of puffing on nutritional composition and phenolic profiles of kiwicha ( Amaranthus caudatus L.) and quinoa ( Chenopodium quinoa Willd.) was investigated. Popped kiwicha showed increased protein and lipid contents and lower contents of carbohydrates compared to the untreated grains. Higher lipid, ash and carbohydrates contents and a decr...

Legumes offer the possibility to develop multifunctional foods with benefits for metabolic syndrome. Our objective was to study the effect of alkaline fermentation by Lactobacillus plantarum and Savinase (FLPS) as well the individual effects of both processes on peptides, phenolics and bioactivity of lentil. FLPS increased peptides and some flavono...

Fermented foods are one of the indispensable components of the dietary culture of every community and among them fermented legumes have been a part of the human diet for centuries. As a staple food, legumes provide nutrients, vitamins and essentials minerals but also phytochemicals associated with non-nutritive attributes that are of interest for h...

Lentil fermentation has a promising potential as a strategy for development of multifunctional ingredients targeting metabolic syndrome (MetS). Response surface methodology was applied to optimize lentil fermentation and study its effects on generation of peptides, soluble phenolics and bioactivities. Fermentation using Lactobacillus plantarum and...

Broccoli consumption is rising worldwide and fertilization is a tool to increase its production. However, little is known about the effect of mineral supplementation to the soil on the bioactive compounds. Therefore, the aim of this investigation was to analyze the content of vitamin C, total phenolic compounds and the antioxidant capacity of brocc...

Germination has been proposed as an economic approach to improve the content of bioactive compounds in pseudocereals. In this work, response surface methodology (RSM) was employed to investigate the impact of germination conditions on the phytochemical content and antioxidant activity of quinoa. The use of desirability methodology showed that the o...

Bioactive peptides are low molecular weight protein fragments of 2–20 amino acids residues that exhibit beneficial physiological effects in vivo. The generation of bioactive peptides during the manufacture of fermented foods is well documented. These peptides are released from the precursor proteins during fermentation or ripening processes by endo...

A wide variability of pulse-derived fermented foods has been available for centuries. The microorganisms involved in legume fermentation hydrolyze and metabolize pulses constituents resulting in the production of valuable products. Pulses fermentation causes desirable changes including the improvement of nutritional value and sensorial properties a...

Sauerkraut is a traditional vegetable product usually produced by spontaneous fermentation that relies on lactic acid bacteria (LAB) naturally present in white cabbage. However, current trends on sauerkraut production propose the application of starter cultures in order to ensure a uniform quality of the product. Besides its high nutritional value,...

Germinated brown rice (GBR) has been suggested as an alternative approach to mitigate highly prevalent diseases providing nutrients and biologically active compounds. In this study, the content of γ-oryzanol, γ-aminobutyric acid (GABA), total phenolic compounds (TPC) and antioxidant activity of soaked (for 24 h at 28°C) and GBR (for 48 and 96 h at...

The objective was to identify peptides with dual antioxidant and angiotensin I converting enzyme (ACE) inhibitory activities released from lentil proteins by Savinase®. The influence of gastrointestinal digestion on peptide bioactivity was also assayed. Fragments from vicilin, convicilin and legumin were the most abundant peptides identified. Pepti...

Fermented Foods in Health and Disease Prevention is the first scientific reference that addresses the properties of fermented foods in nutrition by examining their underlying microbiology, the specific characteristics of a wide variety of fermented foods, and their effects in health and disease. The current awareness of the link between diet and he...

Controlled grain germination is a method of emerging interest to naturally improve nutritional and nutraceutical profile of grains. The aim of this study was to optimize germination temperature and time using response surface methodology to maximize the concentration of γ(phonetic)-amminobutyric acid (GABA), phenolic compounds (PC) and the antioxid...

The objective of this study was to optimize germination time and temperature for maximizing the content of γ-aminobutyric acid (GABA), total phenolic compounds (TPC), and antioxidant activity in purple corn sprouts by using Response Surface Methodology (RSM). The phenolic composition of purple corn sprouts obtained in the optimal conditions was ana...

La presente invención se refiere a un procedimiento sencillo y económico de germinación que, utilizando luz, permite obtener granos de arroz integral germinado y/o productos derivados de los mismos con altos contenidos de compuestos biológicamente activos, preferentemente γ-orizanol y GABA. Los granos de arroz integral germinado obtenidos o los pro...

The aim of this study was to investigate the application of elicitors (500 μM ascorbic acid, 50 μM folic acid, 5 mM glutamic acid and 50 ppm chitosan in 5 mM glutamic acid) during lentil germination up to 8 days as a strategy to increase germination rate and to enhance the accumulation of γ-aminobutyric acid (GABA) and phenolic compounds. The effec...

Europe has become heavily dependent on soya bean imports, entailing trade agreements and quality standards that do not satisfy the European citizen’s expectations. White, yellow, and narrow-leafed lupins are native European legumes that can become true alternatives to soya bean, given their elevated and high-quality protein content, potential healt...

Dear colleagues, It is a pleasure for me to invite you to the next EURO FOOD CHEM XVIII to be held in Madrid, Spain, on the 13 - 16 October 2015. EURO FOOD CHEM XVIII conference will bring together experts from academia, research centres, administration and industries to debate on the latest scientific advances in the field of Food Chemistry cove...

The aim of this work was to investigate the influence of storage at 4 °C in conventional or nitrogen (N2)-enriched atmospheres for 3 months on the microbial status of sauerkraut obtained by natural fermentation or by Leuconostoc mesenteroides inoculation. The content of vitamin C, glucosinolate derivatives and the antioxidant activity of stored sau...

The influence of solid (SSF) or liquid state fermentation (LSF) for 48 and 96 h on the production of water soluble extracts from kidney beans was investigated. SSF was carried out by Bacillus subtilis, whilst LSF was performed either by natural fermentation (NF) or by Lactobacillus plantarum strain (LPF). SSF extracts showed high soluble phenolic c...

Rising prevalence of hypertension is pushing food industry towards the development of innovative food products with antihypertensive effects. The aim was to study the effect of reduced sodium content and 21 % addition of wholemeal wheat sourdough (produced by Lactobacillus brevis CECT 8183 and protease) on proximate composition, γ-aminobutyric acid...

Ready-to-eat sprouts are becoming popular healthy fresh foods. Germination and elicitation may be used to improve phytochemical and functional quality of sprouts. The aim of this study was to unveil the efficacy of germination and the use of elicitors (500 μM ascorbic, 50 μM folic and 5 mM glutamic acid) to enhance the phenolic composition and anti...

The use of enriched Se isotopes as tracers has provided important information on Se metabolism. However, selenium isotopes are expensive and difficult to obtain. A simple and cheap strategy based on the production of [77Se]-methylselenocysteine ([77Se]-MeSeCys) when preparing sauerkraut in the presence of [77Se]-selenite was developed. The resultin...

Germinated brown rice (GBR) is considered healthier than brown rice (BR) but its nutritive value has been hardly studied. Since nutritive quality of GBR depends on genetic diversity and germination conditions, six Ecuadorian BR varieties were germinated at 28 and 34 ºC for 48 and 96 h in darkness and proximate composition, dietary fiber fractions,...

The consumption of seeds of the Fabaceae family is distributed worldwide mainly due to their high content of proteins, carbohydrates, dietary fiber and polyunsaturated fatty acids, jointly with vitamins and minerals. However, they contain several non-nutritive compounds (NNCs) that can exert adverse or beneficial actions upon ingestion depending on...

The influence of germination of kidney beans for 4, 6 and 8 days in different elicitors (ascorbic acid, folic acid, glutamic acid, glutamic acid/chitosan and lactic acid/chitosan) on the production of health-promoting ingredients was studied. Sprouts were characterized according to their protein profile, soluble phenolic compounds (SPC) and γ-amino...

The aim of this study was to produce multifunctional hydrolyzates from lentil protein concentrates. Four different proteases (Alcalase, Savinase, Protamex and Corolase 7089) and different hydrolysis times were evaluated for their degree and pattern of proteolysis, angiotensin I converting enzyme (ACE)-inhibitory and antioxidant activities. Alcalase...

Oligosaccharides are low-molecular-weight carbohydrates widely distributed in nature as plant storage carbohydrates. Fructan-type oligosaccharides (FTO) are widely present in vegetables and cereals while legumes are natural sources of α-galactooligosaccharides (α-GOS) among which raffinose family oligosaccharides (RFO) and galactosyl cyclitols are...

Human consumption of flaxseed is increasing due to its health benefit properties and extrusion processes can enhance its nutritional quality. Extruded flaxseed meal (EFM) obtained in a pilot plant was characterized and incorporated in flour mixes and cereal-based bars to demonstrate its nutritious usefulness. Amino acid content was not affected by...

The aim of this study was to evaluate the effect of rye flour extraction rate on the protein amino acids content and protein quality indexes (chemical score, CS, protein efficiency ratio, PER) of traditional rye bread and ginger cake and to compare them with conventional wheat bread. Rye flour with extraction rates of 1000 g/kg and 920 g/kg (F-1000...

The effect of liquid (LSF) and solid state fermentation (SSF) of lentils for production of water-soluble fractions with antioxidant and antihypertensive properties was studied. LSF was performed either spontaneously (NF) or by Lactobacillus plantarum (LP) while SSF was performed by Bacillus subtilis (BS). Native lactic flora in NF adapted better th...