Juan Arechaga

Juan Arechaga
Universidad del País Vasco / Euskal Herriko Unibertsitatea | UPV/EHU · Laboratory of Stem Cells, Development & Cancer- Department of Cell Biology & Histology- Faculty of Medicine & Dentistry

36.25
 · 
MD-PhD

About

170
Publications
14,879
Reads
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1,601
Citations
Research Experience
March 1988 - present
Universidad del País Vasco / Euskal Herriko Unibertsitatea
Position
  • Professor of Cell Biology
January 1988 - present
Universidad del País Vasco / Euskal Herriko Unibertsitatea
Position
  • Professor of Cell Biology, MD-PhD
July 1984 - December 1985
University of Colorado Medical School, Denver
Position
  • Research Fellow

Publications

Publications (170)
Article
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Abstract Persistent organic pollutants are a group of chemicals that include polychlorinated biphenyls (PCBs). PCBs exposure during adult life increases incidence and severity of cardiomyopathies, whereas in utero exposure determines congenital heart defects. Being fat-soluble, PCBs are passed to newborns through maternal milk, impairing heart func...
Chapter
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Isolation of pools of spermatogenic cells at specific developmental stages is essential for the investigations of molecular events controlling critical transitions during spermatogenesis. Large-scale cell purification techniques allow for combined proteomics, genomics, and transcriptomics studies. Herein, we describe a procedure for the purificatio...
Article
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Nucleoporins are the main components of the nuclear-pore complex (NPC) and were initially considered as mere structural elements embedded in the nuclear envelope, being responsible for nucleocytoplasmic transport. Nevertheless, several recent scientific reports have revealed that some nucleoporins participate in nuclear processes such as transcript...
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The mechanisms implicated in the differentiation of fibroblastic precursors into adipocytes can be analyzed in vitro using cell models, such as the 3T3-L1 cell line. Since cell differentiation involves an exit from the cell cycle, it is likely that molecules that inhibit proliferation participate in the control of adipogenesis. This study was aimed...
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Background Histone deacetylase inhibitors (HDACi) exert multiple cytotoxic actions on cancer cells. Currently, different synthetic HDACi are in clinical use or clinical trials; nevertheless, since both pro-invasive and anti-invasive activities have been described, there is some controversy about the effect of HDACi on melanoma cells. Methods Matrig...
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Today cancer research studies have highlighted the role of the cancer-stroma interaction in the regulation of invasive processes. However, very little is known about cell-to-cell relationships between germinal cancer cells and the somatic ones belong to their close environment, particularly at early invasion stages. Here, we have studied the potent...
Chapter
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Adult stem cells, whose plasticity is variable among different tissues, are essential players in the continued maintenance of tissues and organs. In most cases, adult stem cells are unipotent (i.e. can only originate one specific cell lineage). However, a number of adult stem cell types, such as the hematopoietic ones, show multipotency, and are ab...
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Bordetella pertussis causes whooping cough, a respiratory infectious disease that is the fifth largest cause of vaccine-preventable death in infants. Though historically considered an extracellular pathogen, this bacterium has been detected both in vitro and in vivo inside phagocytic and non-phagocytic cells. However the precise mechanism used by B...
Article
Testicular germ cell tumors (TGCTs) comprise the vast majority of all testicular malignancies and are the most common type of cancer among young male adults. The nonseminomatous variant of TGCTs is characterized by the presence of embryonic and extraembryonic tissues together with a population of pluripotent cancer stem cells, the so-called embryon...
Article
Understanding the mechanisms that enable migrating cells to reach their targets is of vital importance, as several pathologies, including cardiac defects and some tumours, are consequences of altered cell migration. With a view to evaluating if matrix metallo-proteinases (MMPs) and their tissue inhibitors (TIMPs) play a role in the active migration...
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Controversy regarding Open Access movement
Article
Understanding the mechanisms that enable migrating cells to reach their targets is of vital importance, as several pathologies, including cardiac defects and some tumours, are consequences of altered cell migration. With a view to evaluating if matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMPs) play a role in the active migration...
Article
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Spermatogonial stem cells (SSCs) are pluripotent elements found in the adult seminiferous epithelium between Sertoli cells and a basal lamina which covers the multilayered external wall of peritubular myoid cells. The microenvironment of this pluripotent stem cell niche creates the complex and dynamic system that is necessary for the initiation of...
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This interview with a leading figure in human pathology and embryology of mammals is to highlight the close links between the biological interpretation of neoplasms and differentiation processes that normally occur during development, particularly in the case of teratomas. On the other hand, tries to emphasize how work capacity, the firm will to pr...
Article
Cell transplantation into the seminiferous tubules is a useful technique for the study of physiological and pathological conditions affecting the testis. However, the precise three-dimensional organization and, particularly, the complex connectivity of the seminiferous network have not yet been thoroughly characterized. To date, the technical appro...
Article
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Cell transplantation into the seminiferous tubules is a useful technique for the study of physiological and pathological conditions affecting the testis. However, the precise three-dimensional organization and, particularly, the complex connectivity of the seminiferous network have not yet been thoroughly characterized. To date, the technical appro...
Chapter
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Selección de buenas prácticas de revistas españolas: El caso de "the International Journal of Developmental Biology
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The tumour surrounding stroma, known as reactive stroma, is a crucial factor to understand cancer cell growth and invasion. In the normal adult testis, the stroma contains extracellular matrix components, fibroblasts, infiltrating leucocytes, lymphocytes, macrophages and capillaries, as well as other specific cell populations, like Leydig cells and...
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Over the last 15 years, cell transplantation into seminiferous tubules has become a valuable tool to study germinal cell biology and related matters. This is particularly so, because the blood-testis permeability barrier establishes a sealed compartment which protect against certain influences such as immunological rejection. In the light of the fu...
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BILBAO. La mejor revista cientí-fica española lleva el sello de la Uni-versidad del País Vasco (UPV). Es 'The International Journal of De-velopmental Biology' (IJDB). En-cabeza la lista de las publicaciones científicas nacionales a distancia del resto y está entre las diez me-jores de su especialidad, la biología del desarrollo. Su director es el c...
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Background information. The execution phase of apoptosis is characterized by extensive blebbing of the plasma membrane, which usually results in secondary lysis in vitro. To analyse the permeability of cellular membranes during this process, we induced apoptosis in human melanoma A375 cells that had been transfected with fluorescently tagged protei...
Article
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Testicular germ cell tumors (TGCTs) are the most frequent malignancies in adolescents and young adults. The incidence of TGCTs has doubled over the last few decades and the mechanisms underlying their pervasive growth are still poorly understood. Among them, seminomatous and non-seminomatous tumors have carcinoma in situ of the testis (CIS) as a co...
Article
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Testicular germ cell tumours (TGCTs) represent about 2% of male malignancies, being the most common cancer among adolescents and young adults. As in most neoplasias, TGCTs show a chaotic vascular architecture, altered blood supply and over-expression of pro-angiogenic factors, aspects closely related to tumour overgrowth and metastasis. Following t...
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Low oxygen availability (hypoxia) is a hallmark of rapidly proliferating tumors and has been suggested to be a characteristic of the embryonic and adult stem cell niche. The idea of relating cancer to stem cells is increasingly popular due to the identification of specific cancer stem cells sharing the typical plasticity and motility of pluripotent...
Article
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The execution phase of apoptosis is characterized by extensive blebbing of the plasma membrane, which usually results in secondary lysis in vitro. To analyse the permeability of cellular membranes during this process, we induced apoptosis in human melanoma A375 cells that had been transfected with fluorescently tagged proteins which were targeted t...
Article
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Carcinoma in situ (CIS) of the testis is considered to be a precancerous germinal cell lesion, but the precise cellular and molecular mechanisms underlying transformation of CIS into invasive pluripotent cancer cells remain to be elucidated. Moreover, a satisfactory animal model for the experimental study of germinal tumours has not been developed...
Chapter
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Studies on biological development and cancer have pointed out the importance of specific epigenetic environments to maintain the equilibrium between repressed and activated genes. It has been possible to establish that this kind of environment induces chromatin structure modification and heritable changes in gene functions without altering primary...
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If we identify Science and Scientific Research with what has been called Modern Western European Science, it is evident that the Kingdoms of the Iberian Peninsula were not involved significantly in its inception and spread (Basalla, 1967). This might appear to be paradoxical, since the huge Hispanic Empire (which included Portugal and its ultramari...
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We present a survey of the introduction and evolution of microscopy techniques in Spain, and the concepts and lines of research developed around this instrument, particularly in the field of Biomedical research. We cover in our article the long period from the XVII Century to the arrival of the great figure of Santiago Ramon y Cajal (1853-1934). We...
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Francisco Ort-Llorca (1905-1993) was one of the most outstanding Spanish embryologists of the XX century. He was disciple of Henri Rouvire in Paris (France), Alfred Fischel in Vienna (Austria), Walther Vogt in Munich (Germany) and Pedro Ara in Madrid (Spain). From 1935, he was professor of Human Anatomy at the Faculty of Medicine of Cadiz, belonged...
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We revise the historical evolution of the societies devoted to Developmental Biology from the early activities of the Institut International dEmbryologie (IIE), founded in 1911, with particular emphasis on the more recent constitution of the Spanish Sociedad Española de Biología del Desarrollo (SEBD), founded in 1994, and the Portuguese Sociedade P...
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In recent years, the reversion of the cancer phenotype of human melanoma cells in developing zebrafish and chick embryos has been reported. The aim of this review is to revise these and other related contributions regarding the regulation of embryonic cancer and to provide a framework with which to understand results from our laboratory on the inte...
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Epigenetic modifications such as DNA methylation and alterations to chromatin structure have been proposed as hallmarks of imprinting in somatic cells after fertilization. In the germ cell line, gene imprinting needs to be reset in order to transmit the correct sex-specific imprinting pattern to the next generation. The precise timing of imprint er...
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ResumenEn los últimos días hemos sido testigos de la entrega del Premio Príncipe de Asturias de Comunicación y Humanidades a las revistas científicas Nature y Science, auténticos iconos demiúrgicos de una nueva religión intelectual a los que parece que todos los investigadores debemos mostrar adoración y pleitesía. Un aldeanismo más propio de otros...
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Primordial germ cells (PGCs) are the stem cell precursors of the germ line. Several growth factors contribute to enlarging the PGC population by acting as mitogens, survival factors or both. Interleukin-2 (IL-2) has a growth-promoting activity for T and B-lymphocytes, but its role in PGCs had not yet been studied. Here, we show that PGCs isolated f...
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La generalización del uso del inglés como lenguaje científico internacional, la edición electrónica avanzada e interactiva, la venta de paquetes de suscripciones (negociados con universidades, redes de centros o incluso países) y el movimiento Open Access son nuevas estrategias de comunicación, edición, visibilidad y venta de las revistas científic...
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Epigenetic modifications such as DNA methylation and changes in chromatin structure are changes in the chemical composition or structure of DNA that work by regulating gene expression. Their mechanisms of action have been generally studied in imprinted genes. The present work analyzes the involvement of these mechanisms in the expression of the U2a...
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Retinoic acid-induced apoptosis of embryonic stem (ES) cells is an experimental system which resembles the physiological programmed cell death that occurs during differentiation in embryonic development. Our aim was to analyze the involvement of epigenetic modifications such as DNA methylation and chromatin structure in the apoptotic process and to...
Article
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Normalmente, el "producto final" de cualquier tipo de trabajo investigador ¿al menos en las áreas científicas, técnicas o médicas¿ es un artículo original en una revista profesional. Sin embargo, su importancia es algo que no se valora de manera adecuada en nuestro país. Por otra parte, los agentes financiadores de la ciencia en España, tanto públi...
Article
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Testicular germ cell tumors (TGCTs) are the most frequent neoplasia among young people and their incidence has grown very quickly during recent decades in North America and Europe. Many studies have been carried out in order to elucidate the factors involved in the appearance and progression of these tumors. Little is known about the role of cancer...
Article
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Half a century ago, Rupert A. Willis wrote I felt sure that embryologists and pathologists have much to gain by a better knowledge of each other's subjects... pathologists need to know more embryology and embryologists more pathology. Today, in the Molecular Era, we can ratify this idea and say that, in many ways, Oncology and Developmental Biology...
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Rch1 belongs to the importin alpha subfamily and works as an adapter between karyophilic proteins and the nuclear import machinery. Its level of expression varies among species and tissues, and depends on the state of cellular metabolism. In the present study we examined the level of expression of nuclear envelope and nuclear transport proteins (Rc...
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We have studied the effect of N-(4-hydroxyphenyl)retinamide on either malignant human leukaemia cells or normal cells and investigated its mechanism of action. We demonstrate that 4HPR induces reactive oxygen species increase on mitochondria at a target between mitochondrial respiratory chain complex I and II. Such oxidative stress causes cardiolip...
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We have studied the expression of FGF1 and FGF2 during mouse odontogenesis by immunohistochemistry. FGF1 was detected in differentiated odontoblasts and at the secretory pole of ameloblasts. Localization of FGF2 was mainly observed within the basement membrane interposed between dental epithelium and dental mesenchyme. These findings indicate that...
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In the present work, flow cytometry techniques together with morphologic studies were used to perform multiparametric analyses in cell cultures derived from CE44 teratocarcinoma embryoid bodies. The intrinsic cell parameters studied by flow cytometry were size (FALS), cytoplasmic complexity (ISS) and autofluorescence, expressed as LIGFL/FALS (green...
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Nucleoplasmin is a karyophilic protein that is involved in nucleosome formation and decondensation of chromatin, although other precise functions and modes of action of this molecule are still poorly understood. In the present paper we describe a novel nucleocytoplasmic transport assay that has enabled us to study the nuclear distribution of nucleo...
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Spermatogenesis occurs within the testis of adult males by a complex and very well organized process. Breakthroughs in techniques such as cryopreservation and culture of spermatogenic cells and the maturation of these cells in exogenous testes after transplantation renewed the interest in this process. Transplantation of spermatogenic cells from a...