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Introduction
I am a PhD student training in biophysics. Specifically, I'm interested in using cryo-EM to study prokaryotic appendages in pathogenic (Vibrio cholerae) and non-pathogenic bacteria (Caulobacter crescentus). During my training, I hope to better understand workflows and structure determination by cryo-EM.
In my previous research experience at NEIU, I used serial femtosecond x-ray crystallography to study a red-light photoreceptor, called bacteriophytochrome.
Current institution
Publications
Publications (22)
The physiological role of a-synuclein (a-syn), an intrinsically disordered presynaptic neuronal protein, is believed to impact the release of neurotransmitters through interactions with the SNARE complex. However, under certain cellular conditions that are not well understood, a-syn will self-assemble into β-sheet-rich fibrils that accumulate and f...
The physiological role of α-synuclein (α-syn), an intrinsically disordered presynaptic neuronal protein, is believed to impact the release of neurotransmitters through interactions with the SNARE complex. However, under certain cellular conditions that are not well understood, α-syn will self-assemble into β-sheet rich fibrils that accumulate and f...
A number of extracellular helical protein polymers are crucial for supporting bacterial motility. The bacterial flagellum is a polymeric appendage used to support cellular motility. Historically, structural studies of flagellar and other filaments were limited to those present as or locked into straightened states. Here, we present a robust workflo...
Type IV pili (T4P) are ubiquitous in both bacteria and archaea. They are polymers of the major pilin protein, which has an extended and protruding N-terminal helix, α1, and a globular C-terminal domain. Cryo-EM structures have revealed key differences between the bacterial and archaeal T4P in their C-terminal domain structure and in the packing and...
Imaging large fields of view while preserving high-resolution structural information remains a challenge in low-dose cryo-electron tomography. Here we present robust tools for montage parallel array cryo-tomography (MPACT) tailored for vitrified specimens. The combination of correlative cryo-fluorescence microscopy, focused-ion-beam milling, substr...
Flagella are dynamic, ion-powered machines with assembly pathways that are optimized for efficient flagella production. In bacteria, dozens of genes are coordinated at specific times in the cell lifecycle to generate each component of the flagellum. This is the case for Caulobacter crescentus, but little is known about why this species encodes six...
Imaging large fields of view while preserving high-resolution structural information remains a challenge in low-dose cryo-electron tomography. Here, we present robust tools for montage electron tomography tailored for vitrified specimens. The integration of correlative cryo-fluorescence microscopy, focused-ion beam milling, and micropatterning prod...
Titel:
"On the Role of the Conserved Histidine at the Chromophore Isomerization Site in Phytochromes" -
Anastasia Kraskov, David Buhrke, Patrick Scheerer*, Ida Shaef, Juan C. Sanchez, Melissa Carrillo, Moraima Noda, Denisse Feliz, Emina A. Stojković, and Peter Hildebrandt* -
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Phytochromes use light to drive protein structural changes, which i...
Phytochromes are red/far-red light photoreceptors in bacteria to plants, which elicit a variety of important physiological responses. They display a reversible photocycle between the resting Pr state and the light-activated Pfr state. Light signals are transduced as structural change through the entire protein to modulate its activity. It is unknow...
Caulobacter crescentus is a Gram-negative alpha-proteobacterium that commonly lives in oligotrophic fresh and salt-water environments. C. crescentus is a host to many bacteriophages, including ϕCbK and ϕCbK-like bacteriophages, which require interaction with the bacterial flagellum and pilus complexes during adsorption. It is commonly thought that...
29 Phytochromes are red/far-red light photoreceptors in bacteria to plants, which elicit a variety of 30 important physiological responses. They display a reversible photocycle between the resting (dark) 31 Pr state and the light activated Pfr state, in which light signals are received and transduced as 32 structural change through the entire prote...
Cryo-EM and Molecular Biology Approaches for Characterizing Flagellar Filament Structures of Caulobacter Crescentus - Juan Sanchez, Nicoleta Ploscariu, Eric Montemayor, Daniel Parrell, Gan Li, Rebecca Dillard, Conrad Shebelut, Elizabeth Wright
Caulobacter crescentus is a gram-negative alpha-proteobacterium that commonly lives in oligotrophic fresh and salt-water environments. C. crescentus is a host to many bacteriophages, including ϕCbK and ϕCbK-like bacteriophages, which first adsorb to cells by interaction with the bacterial flagellum. It is commonly thought that the six paralogs of t...
Phytochromes (PHYs) are photoreceptor proteins first discovered in plants, where they control a variety of photomorphogenesis events. PHYs as photochromic proteins can reversibly switch between two distinct states: a red light (Pr) and a far-red light (Pfr) absorbing form. The discovery of Bacteriophytochromes (BphPs) in nonphotosynthetic bacteria...
