Juan J Romero

Juan J Romero
National Scientific and Technical Research Council | conicet · INIFTA Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas

PhD in Chemistry

About

16
Publications
1,507
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211
Citations
Introduction
Research associate at INIFTA - CONICET. Our current projects are focused on mechanotransduction in embryonic stem cells and biophysical regulation through external stimulus, using smart materials based on supramolecular chemistry. To understand the underlying chemical and biophysical processes we use fluorescence microscopy, correlation techniques and diverse materials characterization approaches.
Additional affiliations
March 2020 - present
National Scientific and Technical Research Council
Position
  • Research Associate
Description
  • Our projects focus on the regulation of cell differentiation process in embryonic stem cells through changes in the properties of "smart" culture substrates, particularly sensitive to remote stimulus, within the physiological range, and study the mechanisms of cell response to that pertubation. The strategy of construction and use of biomaterials with adjustable properties constitutes an interesting alternative for the extended application of stem cells in regenerative medicine.
April 2017 - March 2020
National Scientific and Technical Research Council
Position
  • PostDoc Position
Description
  • I got a potdoc position at intracellular dyanamics laboratory, under the supervision of prof. Dr. Valeria Levi. Our projects were focused on mechanotransduction in embryonic stem cells, particularly on cytoskeleton coupling to nucleus impacting on pluripotency transcription factors dynamical organization.
March 2016 - April 2016
University of California, Riverside
Position
  • Excange Scholar
Description
  • I was honored with a Fulbright scholarship at the Department ofBioengineering of UCR, under the supervision of prof. Dr. Val Vullev. Our projects were focused on the synthesis and characterization supramolecular systems based on silicon quantum dots for charge separation processes.
Education
March 2012 - March 2017
Universidad Nacional de La Plata
Field of study
  • Chemistry
March 2007 - March 2012
Universidad Nacional de La Plata
Field of study
  • Chemistry

Publications

Publications (16)
Article
Full-text available
Background The cytoskeleton is a key component of the system responsible for transmitting mechanical cues from the cellular environment to the nucleus, where they trigger downstream responses. This communication is particularly relevant in embryonic stem (ES) cells since forces can regulate cell fate and guide developmental processes. However, litt...
Article
Microtubule-dependent motors usually work together to transport organelles through the crowded intracellular milieu. Thus, transport performance depends on how motors organize on the cargo. Unfortunately, the lack of methodologies capable of measuring this organization in cells determines that many aspects of the collective action of motors remain...
Article
Full-text available
In the present study we report the photophysical properties of colloidal ensembles of silicon quantum dots (SiD) and gold nanoparticles (AuNP), particularly focused on investigating metal-enhanced photoluminescence effects. AuNP with different sizes, (27 ± 10) and (88 ± 12) nm, and ca. 3.4 nm size SiD with different surface groups, either covered w...
Article
Pluripotent stem cells (PSCs) are capable of self-renewing and producing all cell types derived from the three germ layers in response to developmental cues, constituting an important promise for regenerative medicine. Pluripotency depends on specific transcription factors (TFs) that induce genes required to preserve the undifferentiated state and...
Article
Grafting of polyethylene glycol (PEG) to ultrasmall photoluminescent silicon dots (SiDs) is expected to improve and expand the applications of these particles to aqueous environments and biological systems. Herein we report a novel one-pot synthesis of robust, highly water compatible PEG-coated SiDs (denoted as PEG-SiDs) of (3.3 ± 0.5) nm size. The...
Chapter
Silicon nanoparticles (including silicon clusters and 1–4 nm size silicon nanocrystals, SiCs) combine size - dependent photoluminescence, the capacity for singlet oxygen and superoxide radical anion generation, and the richness of silicon surface derivatization. Surface modifications as coating/linking with folate, antibodies, adjuvants, and a plet...
Article
Full-text available
Suppressing the charge recombination (CR) that follows an efficient charge separation (CS) is of key importance for energy, electronics and photonics applications. We focus on the role of dynamic gating for impeding CR in a molecular rotor, comprising an electron donor and acceptor directly linked via a single bond. The media viscosity has an unusu...
Article
Metal-enhanced singlet oxygen, O2(¹Δg), generation is a desirable effect to augment the amount of O2(¹Δg) produced upon irradiation of organic sensitizers. Herein, we employed pectin for stabilizing 9 nm silver nanoparticles and showed that these particles are able to form a ground-sate complex with riboflavin (Rf). Pump-probe transient absorption...
Article
Full-text available
The effect of isomeric substitutions on the transmitter for triplet energy transfer (TET) between nanocrystal (NC) donor and molecular acceptor is investigated. Each isomeric acceptor is expected to bind in a unique orientation with respect to the NC donor. We see that this orbital overlap drastically affects the transmission of triplets. Here, two...
Article
Iron-containing silicon nanoparticles were synthesized in an attempt to understand the effect of iron on the silicon nanoparticle (SiNP) photoluminescence and singlet-oxygen generation capacity. A wet chemical oxidation procedure of the sodium silicide precursor, obtained from the thermal treatment in anaerobic conditions of a mixture of sodium, si...
Article
Photoluminescent silicon nanoparticles 1–2 nm in size were synthesized by a wet chemical procedure and derivatized with propylamine (NH2SiNP). Surface NH2 groups were used as linkers for additional poly(ethylene glycol) (PEG) and folic acid (Fo) attachment (PEG-NHSiNP and Fo-NHSiNP, respectively) to enable efficient targeting of the particles to tu...
Article
The effect of derivatization and temperature on the photoluminescence of 1-2 nm size silicon particles of different origin is investigated in an attempt to understand the effect of surface on the particles photoluminescence. To this purpose, silicon nanoparticles were synthesized by electrochemically (top-down) and wet chemical (bottom-up) procedur...
Article
Silicon nanoparticles of 1–5 nm size (SiNPs) were synthesized by a bottom-up (BU) approach involving a chemical wet method. The contribution of different emitters to the overall excitation–emission matrix was analyzed on the assumption that pure substances existing in a unique form show an excitation wavelength-invariant emission spectrum. The occu...

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Project (1)
Project
The general objective of this project is to study the regulation of cell differentiation process in embryonic stem cells through changes in the properties of "smart" culture substrates, particularly sensitive to remote stimulus (pH, temperature, ionic strength, radiation, release of analytes) within the physiological range, and study the mechanisms of cell response to that pertubation. The strategy of construction and use of biomaterials with adjustable properties constitutes an interesting alternative to extend the application of stem cells in regenerative medicine.