Juan L. Pérez-Rodríguez

• Doctor of Philosophy
• Curator of the Nicotiana germplasm bank at Tobacco Research Institute, Cuba

Maturity of the seeds can affect their potential longevity. It has been shown that components related to seed quality develop after the moment when the seed has reached its maximum dry mass, known as harvest maturity. In this state seeds have already developed critical adaptation mechanisms, such as detoxification of reactive oxygen species (ROS),...

The examination of genetic stability is one of the most important issue in the evaluation of cryopreservation techniques. This research was aimed at determining if there are changes at the phenotypic and molecular level in tobacco plants derived from cryopreserved seeds. The seeds of the cultivar "Sancti Spíritus 96" were harvested 35 d after anthe...

EXCELENTE TRABAJO METODOLOGICO

Cryopreservation is a valuable technique for the long-term conservation of plant germplasm and complementary to traditional seed storage methods. However, critical factors such as seed moisture content should be optimized before using this technique as a safe strategy for storing seeds such as those of Nicotiana spp. This study aimed to determine t...

There have been carried out several investigations dealing with criopreservation as an alternative to seeds storage traditional methods all around the world. Seed cryopreservation humidity has been identified as a decisive factor in its longevity. Nevertheless, there is not enough information about the effect of the drying on physiologic and bioche...

The main objective of this investigation was to determine the effect of the ripening in the cryopreservation of the Nicotiana tabacum L seeds. cv Sancti Spíritus 96 (SS-96). This study was done between the years 2012 and 2014, at the Tobacco experimental station located in Cabaiguan, Sancti Spiritus. SS-96 seeds were harvest at the 14, 21, 28, 35,...

In Cuba, conservation of Nicotiana tabacum is vital because it is one of the main items on the export and sustainability of the economy. The experimental station of Cabaiguán in Sancti Spiritus, whose functions include the conservation of genetic material from Nicotiana tabacum. Due to the small size of these seeds (0,1-0,5 mm), quantification has...

El falso Orobanche afecta a la producción tabacalera de Cuba. Las plantas afectadas desarrollan abundante proliferación de brotes y tumores en las raíces, muestran enanismo y raquitismo. Los cultivares de tabaco Negro cubano son susceptibles a Nocardia sp., agente causal de esta enfermedad. L a incorporación de genes de resistencia a este agente fi...

In the germplasm bank of the Tobacco Research Institute of Cuba the germinative power was determined in 11 species belonging to the genus Nicotiana. All the evaluated species presented high dormancy degree which obtained less than 40% of germination. For the species Nicotianamegalosiphon Heurck and Mueller the effect of potassium nitrate (3.0 g/L)...