Juan Pablo PavissichUniversidad Adolfo Ibáñez · Facultad de Ingeniería y Ciencias
Juan Pablo Pavissich
PhD
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21
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Publications (21)
A novel biofilm model is described which systemically couples bacteria, extracellular polymeric substances (EPS) and solvent phases in biofilm. This enables the study of contributions of rheology of individual phases to deformation of biofilm in response to fluid flow as well as interactions between different phases. The model, which is based on fi...
A novel continuous flow system with "flat geometry" composed by two completely mixed aerobic tanks in series and a settler was used to promote the formation of aerobic granular sludge. Making similarities of this system with a typical sequencing batch reactor (SBR), for aerobic granules cultivation, the value of the tank 1/tank 2 vol ratio and the...
The membrane-aerated biofilm reactor (MABR) is a novel wastewater treatment technology based on oxygen-supplying membranes. The counter diffusion of oxygen and electron donors in MABRs leads to unique behavior, and we hypothesized it also could impact predation. We used optical coherence tomography (OCT), microsensor analyses, and mathematical mode...
The mechanical properties of biofilms can be used to predict biofilm deformation under external forces, for example, under fluid flow. We used magnetic tweezers to spatially map the compliance of Pseudomonas aeruginosa biofilms at the microscale, then applied modeling to assess its effects on biofilm deformation. Biofilms were grown in capillary fl...
This study used culture-dependent and culture-independent approaches to characterize bacterial communities in copper plumbing corrosion and to assess biofilm formation and copper resistance of heterotrophic bacteria isolated from copper pipes.
Water and copper pipes were collected from a cold-water household distribution system affected by 'blue wa...
We used bench-scale tests and mathematical modeling to explore chemical oxygen demand (COD) removal rates in a moving-bed biofilm reactor (MBBR) for winery wastewater treatment, using either urea or nitrate as a nitrogen source. With urea addition, the COD removal fluxes ranged from 34 to 45 gCOD/m2-d. However, when nitrate was added, fluxes increa...
Retrofitting wastewater treatment plants with continuous aerobic granular sludge reactors is a promising alternative to enhance treatment capacities and reduce footprint. This study investigates the main variables influencing granulation and microbial dynamics in two reactor configurations (25 L): stirred tanks in series (R1) and a plug-flow-like s...
Cyclic episodes of granules formation and disintegration took place in two lab-scale aerobic granular sludge sequencing batch reactors, one fed with synthetic wastewater (COD: 0.6 g L-1 and NH4+-N: 0.06 g L-1) and operated at a constant organic loading rate (2.5 g COD per L d), and the other fed with real wastewater (soluble COD: 0.27-1.37 and NH4+...
Nitrification and sulfur-based autotrophic denitrification processes can be used to remove ammonia from wastewater in an economical way. However, under certain operational conditions, these processes accumulate intermediate compounds, such as elemental sulphur, nitrite, and nitrous oxide, that are noxious for the environment. In order to predict th...
Anaerobic digestion (AD) is commonly used for the stabilization of agro-food wastes and recovery of energy as methane. Since AD removes organic C but not nutrients (N and P), additional processes to remove them are usually applied to meet the stringent effluent criteria. However, in the past years, there was a shift from the removal to the recovery...
Peracetic acid (PAA) is an alternative disinfectant that may be effective for combined sewer overflow (CSO) disinfection, but little is known about the effect of particle size on PAA disinfection efficiency. In this work, PAA and hypochlorite were compared as disinfectants, with a focus on the effect of wastewater particles. Inactivation experiment...
Corrosion of copper pipes may release high amounts of copper into the water, exceeding the maximum concentration of copper for drinking water standards. Typically, the events with the highest release of copper into drinking water are related to the presence of biofilms. This article reviews this phenomenon, focusing on copper ingestion and its heal...
Microbially influenced corrosion (MIC) is recognized as an unusual and severe type of corrosion that causes costly failures around the world. A microbial biofilm could enhance the copper release from copper plumbing into the water by forming a reactive interface. The biofilm increases the corrosion rate, the mobility of labile copper from its matri...
We present a novel approach, based on image analysis and modelling, to study the impact of morphological variability (roughness) and fluid dynamics on substrate mass fluxes in biofilms. Specifically, we used this method to assess substrate fluxes in counter-diffusional autotrophic biofilms in a hydrogen-based membrane biofilm reactor. The physical...
We studied the abundance and diversity of the sulfate-reducing prokaryotes (SRPs) in two 30-cm marine chilean sediment cores, one with a long-term exposure to copper-mining residues, the other being a non-exposed reference sediment. The abundance of SRPs was quantified by qPCR of the dissimilatory sulfite reductase gene β-subunit (dsrB) and showed...
Many utilities are seeking more cost effective, safe, and reliable electron donors for biological nutrient removal (BNR). Elemental sulfur (S°) may be an attractive option, but little is known about the feasibility of S°-based denitrification and its kinetics. In this research,
we used bench–scale, S° packed–bed reactors to determine denitrificatio...
Standard measurements of dissolved copper are made by filtering water samples through 0.45 μm pore-size membranes. However, the surface of corroding metallic surfaces may be covered by topographic features < 0.2 μm and structures that can be detached into the bulk water as nano-sized particles. A SEM, EDX, and AFM characterization of a corroding pi...
In the last decades, the worldwide increase in copper wastes release by industrial activities like mining has driven environmental metal contents to toxic levels. For this reason, the study of the biological copper-resistance mechanisms in natural environments is important. Therefore, an appropriate molecular tool for the detection and tracking of...
Sulfate-reducing bacterial communities from coastal sediments with a long-term exposure to copper (Cu)-mining residues were studied in lactate enrichments. The toxicity of excess copper may affect sulfate-reducing bacterial communities. Sulfate reduction was monitored by sulfate and organic acid measurements. Molecular diversity was analyzed by 16S...