Juan Mosqueda

Juan Mosqueda
Autonomous University of Queretaro · Natural Sciences College

Professor

About

240
Publications
46,666
Reads
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2,052
Citations
Citations since 2017
53 Research Items
1190 Citations
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2017201820192020202120222023050100150200250
2017201820192020202120222023050100150200250
2017201820192020202120222023050100150200250

Publications

Publications (240)
Article
Full-text available
Bovine babesiosis is a tick-borne disease caused by protozoan parasites of the genus Babesia . Babesia bigemina is one of the most prevalent and economically important parasite species that infects cattle because of its impact on the meat and milk production industry. Effective disease control strategies should include detection of reservoir animal...
Article
Full-text available
The tick-transmitted disease bovine babesiosis causes significant economic losses in many countries around the world. Current control methods include modified live-attenuated vaccines that have limited efficacy. Recombinant proteins could provide effective, safe, and low-cost alternative vaccines. We compared the expression of the Babesia bovis thr...
Article
Full-text available
We aim to provide a harmonized view of the factors that affect the survival and promote the spread of R. microplus in the Neotropics, approaching its different facets of biology, ecology, distribution, and control. We review the interactions among environmental niche, landscape fragmentation, vegetal coverage (abiotic traits), and the biotic aspect...
Article
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En marzo 2020, la Organización Mundial de la Salud declaró la pandemia de la enfermedad por Coronavirus-2019 (COVID-19), la cual es ocasionada por el Coronavirus tipo 2 que ocasiona el síndrome respiratorio agudo (SARS-CoV�2). Hasta mayo 2022, esta enfermedad ha ocasionado 5,725,075 casos acumulados y 323,891 muertos, reportando en promedio 464 cas...
Article
Full-text available
In B. bigemina, the 45 kilodaltons glycoprotein (GP-45) is the most studied. GP-45 is exposed on the surface of the B. bigemina merozoite, it is believed to play a role in the invasion of erythrocytes, and it is characterized by a high genetic and antigenic polymorphism. The objective of this study was to determine if GP-45 contains conserved B-cel...
Article
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This study aimed to investigate the effect of feeding insoluble fiber on the microbiota and metabolites of the caecum and feces of rabbits recovering from epizootic rabbit enteropathy relative to non-infected rabbits. Rabbits that had either recovered from epizootic rabbit enteropathy or ones that had never had epizootic rabbit enteropathy were fed...
Preprint
This study addresses the variability of the mitochondrial cytochrome oxidase subunit I (COI) and 16S rDNA (16S), and nuclear internal transcriber spacer ITS2 (ITS2) genes in a set of purposely collected samples of the cattle tick, Rhipicephalus microplus (Canestrini, 1888) introduced in the Nearctic-Neotropical range (Mexico to Argentina), and in g...
Article
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Equine piroplasmosis is a disease of horses, mules and donkeys, caused by the hemoprotozoans Babesia caballi and Theileria equi and transmitted by ticks of tropical and subtropical regions. Because the clinical signs are not specific, the diagnosis of equine piroplasmosis is difficult. In Mexico, where the environmental factors are conducive to the...
Article
Full-text available
Babesia and Theileria are apicomplexan parasites that cause established and emerging diseases in humans, domestic and wild animals. These protozoans are transmitted by Ixodid ticks causing babesiosis or theileriosis, both characterized by fever, hemolytic anemia, jaundice, and splenomegaly. In North America (NA), the most common species affecting h...
Article
Vaccines against bovine babesiosis must, ideally, induce a humoral immune response characterized by neutralizing antibodies against conserved epitopes and a cellular Th1 immune response. In Babesia bovis, proteins such as AMA-1, MSA-2c, and RAP-1 have been characterized and antibodies against these proteins have shown a neutralizing effect, demonst...
Preprint
Full-text available
Background: This study aimed to investigate the effect of feeding insoluble fibre on the microbiome and metabolites of the caecum and faeces of rabbits recovering from epizootic rabbit enteropathy relative to non-infected animals. Methods: Animals which had either recovered from epizootic rabbit enteropathy, or ones which had never had epizootic ra...
Article
Full-text available
The objective of this study was to evaluate the effectiveness of the main anthelmintics used for the control of trematodes in cattle in an endemic area in the humid Mexican tropics. A diagnostic study was carried out in nine cattle farms to determine the prevalence of flukes through faecal examination. Only three farms with more than 20 cows positi...
Article
Full-text available
With the worldwide development of anthelmintic resistance, new alternative approaches for controlling gastrointestinal nematodes in sheep are urgently required. In this work, we identified and characterized native nematode-trapping fungi. We collected seven isolates of fungi with the capacity to form adhesive, three-dimensional networks as the main...
Conference Paper
Full-text available
Tepary bean (Phaseolus acutifolius) lectins exhibit differential in vitro cytotoxicity and in vivo antitumorigenic effects against colon cancer by oral administration, but their gastrointestinal interactions and digestion have not yet been assessed. This work evaluated the changes of a recombinant Tepary bean lectin (rTBL-1) through an in vitro/ex...
Article
Full-text available
Tepary bean (Phaseolus acutifolius) lectins exhibit differential in vitro and in vivo biological ef-fects, but their gastrointestinal interactions and digestion have not yet been assessed. This work aimed to evaluate the changes of a recombinant Tepary bean lectin (rTBL-1) through an in vitro and ex vivo gastrointestinal process. A polyclonal antib...
Article
Full-text available
Tick vaccination is an environmentally friendly alternative for tick control, pathogen infection, and transmission. Tick vaccine protection is sometimes incomplete, which may be due to problems in the stability, conformation, and activity of antibodies. This might be related to oxidative stress, but more studies are needed about the possible relati...
Article
Full-text available
Bovine babesiosis is a reportable transboundary animal disease caused by Babesia bovis and Babesia bigemina in the Americas where these apicomplexan protozoa are transmitted by the invasive cattle fever ticks Rhipicephalus (Boophilus) microplus and Rhipicephalus (Boophilus) annulatus. In countries like Mexico where cattle fever ticks remain endemic...
Article
Theileria cervi is a tick-borne protozoan that infects different deer species around the world. Clinical diseases due to Theileria cervi have been reported in white-tailed deer (Odocoileus virginianus) and mule deer (Odocoileus hemionus) in the USA, however, information about this parasite has not been documented in Mexico. Here, blood samples from...
Article
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The invasive cattle fever tick, Rhipicephalus (Boophilus) microplus (Canestrini) (Acari: Ixodidae), occurs in the United States only along the Texas border as incursions from Mexico. Intensive acaricide treatment of cattle herds in Mexico to reduce tick populations has resulted in the development of resistance to an array of acaricides. Resistance...
Article
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Cinnamomum verum is a commonly used herbal plant that has several documented properties against various diseases. The existing study evaluated the inhibitory effect of acetonic extract of C. verum (AECV) and ethyl acetate extract of C. verum (EAECV) against piroplasm parasites in vitro and in vivo. The drug-exposure viability assay was tested on Ma...
Article
Climatic changes have influenced the temporal and spatial distribution of diseases. In livestock‐grazing areas, rodents are reservoirs of zoonotic pathogens; therefore, they play an important role in the transmission of diseases affecting domestic animals and humans. The objective of this study was to investigate the presence of the zoonotic agents...
Article
Full-text available
Ticks and transmitted pathogens constitute a major burden for cattle industry in the Neotropics. To address this limitation, the Spanish Ibero-American Program of Science and Technology in Development office (CYTED) supported from 2018 a network of scientists named "LaGar" (CYTED code 118RT0542) aimed at optimizing the control strategies of cattle...
Article
Absence of an effective high-throughput drug-screening system for Babesia parasites is considered one of the main causes for the presence of a wide gap in the treatment of animal babesiosis when compared with other hemoprotozoan diseases, such as malaria. Recently, a simple, accurate, and automatic fluorescence assay was established for large-scale...
Article
Full-text available
The datasets of records of the distribution of ticks and their hosts are invaluable tools to understand the phylogenetic patterns of evolution of ticks and the abiotic traits to which they are associated. Such datasets require an exhaustive collection of bibliographical references. In most cases, it is necessary the confirmation of reliable identif...
Article
Full-text available
Introducción: Las garrapatas Rhipicephalus microplus son ectoparásitos hematófagos que impactan económicamente la ganadería a nivel mundial. Los acaricidas han sido una estrategia de control efectiva pero que generan la resistencia a estos químicos y la contaminación del medio ambiente. Una alternativa para contrarrestar los daños adversos de los q...
Article
As an emerging disease, the porcine epidemic diarrhea virus has caused substantial economic losses to the pork industry in Mexico, leading to piglet mortality rates of up to 100%. For detection, sequencing, and genetic characterization of the virus, 68 samples of one‐week‐old piglets from pork farms in 17 states of Mexico were analyzed. In total, 5...
Article
Full-text available
Bovine babesiosis is the most important protozoan disease transmitted by ticks. In Plasmodium falciparum, another Apicomplexa protozoan, the interaction of rhoptry neck protein 2 (RON2) with apical membrane antigen-1 (AMA-1) has been described to have a key role in the invasion process. To date, RON2 has not been described in Babesia bigemina, the...
Article
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Sexual stage induction under in vitro conditions is useful for biological and molecular studies of Babesia parasites. Therefore, in the present study, we induced B. ovata tick stages using the chemical inducers: xanthurenic acid (XA), dithiothreitol (DTT) and tris (2-carboxyethyl) phosphine (TCEP) at 27 °C or 37 °C conditions. Cultures at low tempe...
Article
Full-text available
This randomized, double-blind, parallel and placebo-controlled study aimed to evaluate the effect of Bacillus coagulans GBI-30, 6086® probiotic (GanedenBC30®) against upper respiratory tract infections (URTI) and gastrointestinal tract infections (GITI) in eighty healthy school-aged children (6–8 years old). The participants received daily a sachet...
Article
Full-text available
The apical membrane antigen 1 (AMA‐1) is a protein of the micronemes that is present in all organisms of the phylum Apicomplexa; it has been shown that AMA‐1 plays an essential role for parasite invasion to target cells. It has been reported that AMA‐1 is conserved among different isolates of Babesia; however, it is unknown whether the protein cont...
Article
Full-text available
The fecal virome comprises a complex diversity of eukaryotic viruses, phages and viruses that infect the host. However, little is known about the intestinal community of viruses that is present in wild waterfowl, and the structure of this community in wild ducks has not yet been studied. The fecal virome compositions of six species of wild dabbling...
Data
Virus species identified in feces of wild ducks. Sequences classified as viruses were compared to a viral protein database using BLASTx. (DOCX)
Data
Rarefaction curves for 12 fecal samples. Rarefaction curves from the BLASTn search were created with MEGAN at the family level. (TIF)
Article
Full-text available
Background Babesia bovis belongs to the phylum Apicomplexa and is the major causal agent of bovine babesiosis, the most important veterinary disease transmitted by arthropods. In apicomplexan parasites, the interaction between AMA1 and RON2 is necessary for the invasion process, and it is a target for vaccine development. In B. bovis, the existence...
Conference Paper
Full-text available
By using bioinformatics, we developed an accurate, fast and cheap methodology to generate antibodies against the bovine receptor CD205 with detection levels matching those of commercial monoclonal antibodies.
Article
Full-text available
Babesia bigemina is one of the most prevalent species causing bovine babesiosis around the world. Antigens involved in host cell invasion are vaccine targets for this disease but are largely unknown in this species. The invasion process ofBabesiaspp. into erythrocytes involves membrane proteins from the apical complex. A protein stored in the micro...
Article
Full-text available
The cattle industry is one of the most important agroeconomic activities in Mexico. The national herd is estimated to include approximately 33.5. million head of cattle. Ticks and tick-borne diseases are principal factors with a negative impact on cattle health and production. The most economically important tick species parasitizing cattle in Mexi...
Article
Full-text available
Ixodicides resistance of ticks is one of the most important problems for the livestock industry in tropical and subtropical regions, mainly due to the increase in cases of multiple resistance in all families of the ixodicides used. Molecular markers such as single nucleotide polymorphisms (SNPs) has been proposed to identify the resistance to ixodi...
Article
Full-text available
Ixodicides resistance of ticks is one of the most important problems for the livestock industry in tropical and subtropical regions, mainly due to the increase in cases of multiple resistance in all families of the ixodicides used. Molecular markers such as single nucleotide polymorphisms (SNPs) has been proposed to identify the resistance to ixodi...
Article
Full-text available
Background Bovine babesiosis is a tick-borne disease caused by the protozoan parasites of the genus Babesia. In their host vector, Babesia spp. undergo sexual reproduction. Therefore, the development of sexual stages and the subsequent formation of the zygote are essential for the parasite to invade the intestinal cells of the vector tick and conti...
Article
Vector-borne hemoparasitic infections are a major problem that affects livestock industries worldwide, particularly in tropical and subtropical regions. In this work, a reverse line blot (RLB) hybridization assay was developed for the simultaneous detection and identification of Anaplasma, Babesia and bovine trypanosomes, encompassing in this way t...
Poster
Full-text available
Vector-borne hemoparasitic infections are a major constraint for livestock production worldwide, particularlly in tropical and subtropical regions. Given the importance of epidemiological surveilliance of these pathogens, a reverse line blot (RLB) hybridization assay for detection of trypanosomes of veterinary relevance was developed and further co...
Conference Paper
Full-text available
RESUMEN: El presente estudio describe el caso de varios terafósidos criados en cautiverio en Querétaro, infestados por tres grupos de parásitos: nematodos (Rhabditida), moscas Megaselia scalaris (Diptera: Phoridae) y un tipo de bacteria Gram (+). Se analizaron tarántulas con diferente signos dependiendo del tipo de parásito o microorganismo, alguna...
Article
Full-text available
Mexico has a long history of parasitological studies in communities of vertebrates. However, the mega diversity of the country makes fauna inventories an ongoing priority. Presently, there is little published on the parasite fauna of gray foxes (Urocyon cinereoargenteus Schereber, 1775) and this study provides new records of parasites for gray foxe...
Conference Paper
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A meeting sponsored by the Bill & Melinda Gates Foundation was held at the Avanti Hotel, Mohammedia, Morocco, July 14–15, 2015. The meeting resulted in the formation of the Cattle Tick Vaccine Consortium (CATVAC).
Article
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The molecular mechanisms involved during the infection of Rhipicephalus microplus midgut cells by Babesia bigemina are of great relevance and currently unknown. In a previous study, we found a voltage-dependent anion channel (VDAC)-like protein (BmVDAC) that may participate during parasite invasion of midgut cells. In this work, we investigated BmV...
Conference Paper
Full-text available
2 Phileo Lesaffre Animal Care, Mexico Babesia bigemina is one of the causative agents of Bovine babesiosis in temperate regions of the world, including the Americas. It has been shown that a Th1 response correlates with protection in infected cattle. Immunodominant antigens of B. bigemina are highly variable, however conserved, subdominant epitopes...
Article
Full-text available
Strain superinfection occurs when a second strain infects a host already infected with and having mounted an immune response to a primary strain. The incidence of superinfection with Anaplasma marginale, a tick-borne rickettsial pathogen of domestic and wild ruminants, has been shown to be higher in tropical versus temperate regions. This has been...
Article
Full-text available
Strain superinfection occurs when a second strain infects a host already infected with and having mounted an immune response to a primary strain. The incidence of superinfection with Anaplasma marginale, a tick-borne rickettsial pathogen of domestic and wild ruminants, has been shown to be higher in tropical versus temperate regions. This has been...
Article
Full-text available
We harvested 21 fallow deer (Dama dama) and 17 axis deer (Axis axis) in northern Mexico. Two fallow deer were positive for Babesia bigemina and one for Babesia bovis. Amplicons had the expected 170 and 291 base pairs and were identical to B. bigemina (S45366) and B. bovis (M38218), respectively.
Conference Paper
Rhipicephalus microplus is an invasive tick that vectors Babesia bovis and B. bigemina, the protozoans that cause cattle fever. Acaricide resistant R. microplus populations are a major problem for many cattle producing areas of the world. Pyrethroid resistance in arthropods is typically based on mutations in domains I, II, and III of voltage-gated...
Article
Full-text available
Strain superinfection occurs when a second pathogen strain infects a host already infected with a primary strain. The selective pressures that drive strain divergence, which underlies superinfection, and allow penetration of a new strain into a host population are critical knowledge gaps relevant to shifts in infectious disease epidemiology. In reg...
Article
Full-text available
Background Acaricide resistant Rhipicephalus microplus populations have become a major problem for many cattle producing areas of the world. Pyrethroid resistance in arthropods is typically associated with mutations in domains I, II, III, and IV of voltage-gated sodium channel genes. In R. microplus, known resistance mutations include a domain II c...
Article
Ticks are obligate haematophagous ectoparasites considered the principal vectors of disease among animals. Rhipicephalus microplus and R. annulatus ticks are the most important vectors for Babesia bigemina and B. bovis, two of the most important intraerythrocytic protozoan parasites species in cattle, responsible for babesiosis which together with...