Juan G. Maldonado-EstradaUniversity of Antioquia | UdeA · Facultad de Ciencias Agrarias
Juan G. Maldonado-Estrada
Professor
Since October 18, 2024 I am the University of Antioquia Veterinary Hospital Medical Director (Small Animal Practice).
About
133
Publications
225,269
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941
Citations
Introduction
I obtained my Veterinarian degree in 1989—my Master of Science degree in 1993, and my Ph.D. in 2002. I started my work as an assistant professor in Theriogenology (October 1995) at the College of Veterinary Medicine, University of Antioquia (Colombia). I have worked in undergraduate and graduate courses, research, and community services. I held my Professor status in December 2010. Our research, teaching, and outreach activities focus on large and small animal Theriogenology.
Additional affiliations
October 2024 - present
Position
- Medical Director of the Veterinary Hospital
Description
- Chief Veterinary Medical Officer (Small Animals)
January 2019 - June 2019
Position
- Professor
Description
- Teaching bovine and canine theriogenology
January 2018 - January 2018
Position
- Master's Student
Description
- Teaching activities in the Small Animal Obstetrics and Gynecology module (Unit).
Education
September 1999 - November 2002
August 1990 - May 1993
August 1990 - May 1993
Publications
Publications (133)
There is poor agreement between clinical findings and results of diagnostic tests in postpartum uterine diseases that may be explained at least in part by the presence of inflammation and infection at sites other than the endometrium. It was hypothesized that clinical cervicitis and vaginitis are components of reproductive tract inflammatory disord...
No studies have evaluated the peripartum follicular dynamics resulting in foal heat under tropical environments. We aimed to assess retrospectively the peripartum follicular dynamics in Colombian Paso Fino mares that were inseminated at the foal heat, becoming pregnant or not. Records including follicular dynamics of pregnant mares prepartum and fr...
Uterine involution, ovarian activity, and incidence of postpartum uterine disease (PUD) were assessed in forty-eight dairy cows from calving until the 10th postpartum week. Postpartum follow-up included evaluation of uterine involution and ovarian structures by B-mode, Doppler color, and Doppler spectral ultrasound of the right uterine artery in co...
This study aims to assess the acquisition of surgical skills for laparoscopic ovariectomy (LOE) in dogs by veterinary surgeons with no experience in minimally invasive surgery using the CALMA Veterinary Lap-trainer simulator (CVLTS) in an experimental and analytical setting. Veterinary surgeons with no experience in minimally invasive surgery (MIS)...
Preeclampsia is a pregnancy-related multisystem disorder characterized by altered trophoblast invasion, oxidative stress, exacerbation of systemic inflammatory response, and endothelial damage. The pathogenesis includes hypertension and mild-to-severe microangiopathy in the kidney, liver, placenta, and brain. The main mechanisms involved in its pat...
The validity of the CALMA Veterinary Lap-trainer simulator (CVLTS) for training basic veterinary laparoscopic skills was assessed and compared to a simple collapsible mobile box trainer. Ten veterinarian surgeons with no experience in laparoscopic surgery and four experts with at least two years of experience in minimally invasive surgery (MIS) wer...
Background. Currently, legal limitations avoid repetition and deliberate practice on cadavers and experimental models, which are fundamental for minimally invasive surgery skills acquisition. The CALMA Veterinary Lap-trainer (CVLTS) simulator is an ergonomic canine abdominal model developed to allow training of basic and advanced laparoscopic skill...
The development of innovative simulation models for veterinary laparoscopic surgery training is a priority today. This study aimed to describe a didactic simulation tool for the training of total laparoscopic gastropexy (TLG) with intracorporeal sutures in dogs. CALMA Veterinary Lap-trainer composite simulator (CLVTS) was developed from a plaster c...
El presente marco de referencia del módulo Salud y Bienestar Animal del examen Saber Pro fue elaborado por el profesor Juan Guillermo Maldonado Estrada. Su construcción se
llevó a cabo con base en una versión de 2016. Esta última revisión se ajusta a los lineamientos para la elaboración y revisión de marcos de referencia y busca actualizar la infor...
The field of Reproductive Immunology has evolved from the paradigms of transplantation immunology to an integrated concept of interactions between the endocrine, immune, nervous, and reproductive systems as critical components of the physiology of reproduction. The origin of reproductive immunology was the Medawar paradox when he proposed several t...
Sperm motility is one of the most relevant parameters related to the fertilizing capability of the spermatozoa. Understanding the anatomy and physiology of sperm flagellum allows for the use of diverse molecules for improving sperm motility, such as metformin. Metformin is a molecule known to stimulate the capability of the cell to uptake and metab...
Sperm motility is one of the most relevant parameters related to the fertilizing capability of the spermatozoa. Understanding the anatomy and physiology of sperm flagellum allows for the use of diverse molecules for improving sperm motility, such as metformin. Metformin is a molecule known to stimulate the capability of the cell to uptake and metab...
Sperm motility is one of the most relevant parameters related to the fertilizing capability of the spermatozoa. Understanding the anatomy and physiology of sperm flagellum allows for the use of diverse molecules for improving sperm motility, such as metformin. Metformin is a molecule known to stimulate the capability of the cell to uptake and metab...
Early embryo development is driven first by the maternal RNAs and proteins accumulated during the oocyte’s cytoplasmic maturation and then after the embryo genome activation. In mammalian cells, ATP generation occurs via oxidative pathways or by glycolysis, whereas in embryonic stem cells, the consumption of glucose, pyruvate, lipids, and amino aci...
The cow's uterus sustains bacterial contamination and active inflammation after calving as part of the normal physiological process of uterine involution. Although the definition, incidence, and risk factors for postpartum uterine diseases (PUD) have been documented in annual calving systems with high-producing cows on total mixed ration, the liter...
Introducción
En todo proceso de enseñanza-aprendizaje la evaluación de la formación conceptual y la adquisición de habilidades y destrezas de un aprendiz debe estar encaminada a verificar si con la educación recibida se adquirieron los conocimientos y destrezas requeridos para asumir su desempeño profesional.
El módulo Salud y Bienestar Animal está...
Infertility is a common consequence of cows suffering from postpartum uterine diseases. Diseases from a uterine origin in early lactation affect both uterine and ovarian tissues decreasing the probability of pregnancy. The objective of the present study was to determine the impact of clinical metritis, clinical endometritis, and subclinical endomet...
Infertility is a common consequence of cows suffering from postpartum uterine diseases. Diseases from a uterine origin in early lactation affect both uterine and ovarian tissues decreasing the probability of pregnancy. The objective of the present study was to determine the impact of clinical metritis, clinical endometritis, and sub-clinical endome...
New evidence suggests that glycan expression in placental cells of women with invasive disorders of pregnancy differs from that in normal pregnant women. Hypothesizing that modifications of glycan expression could account for the course of preeclampsia, we established placental villous histocultures and compared glycan expression in women with pree...
The in vitro embryo production industry in the actual world presents some difficulties related to low embryonic production rates, a problem that could be associated with in vitro culture conditions that differed from the in vivo (oviductal) conditions, mainly related to cytoplasmic lipid accumulation. L-carnitine is known as a modulator of β-oxidat...
l-carnitine is a potent antioxidant used for in vitro culture systems. Controversial results have been reported using l-carnitine in culture medium at different stages of in vitro bovine embryo production. Cumulus-oocyte complexes (n = 843) were in vitro-fertilized and cultured and added (treatment group) or not added (control group) with l-carniti...
At present, veterinary laparoscopic surgery training is lacking in experiences that provide a controlled and safe environment where surgeons can practice specific techniques while receiving experts' feedback. Surgical skills acquired using simulators must be certified and transferable to the operating room. Most models for practicing laparoscopic s...
Improving the early detection of animals or herds at increased risk for diseases, reduced milk yield or impaired reproductive performance should be an essential component of herd health programs. The present study aimed to describe the findings of hematological, biochemical, and milk analytes of grazing cows from tropical dairy herds during the ear...
Subclinical Ruminal Acidosis (SARA) and Subclinical Ketosis (SCK) are two of the most prevalent metabolic diseases of dairy cows, with impacts on reproductive performance. There is scarce literature about these diseases in dairy regions in Colombia. In 29 randomly selected herds in Pasto, Colombia, 249 dairy cows were followed weekly for two months...
Background: The diagnosis of ovarian tumors in dogs is usually complicated because the clinical signs can be very discrete and can be easily confused with other diseases. There are few reports of ovarian tumors with different cellular characteristics in the same dog. Our objective was to describe an unusual case of the concomitant presence of ovari...
Skin prick-test is the first choice for the detection of type I hypersensitivity in human atopic dermatitis. Canine atopic dermatitis resembles several symptoms of the disease in the human counterpart. In canine atopic dermatitis, intradermal testing is the test of choice, and there are few reports on the use of skin prick test (SPT) in dogs. The p...
Aim: This study aimed to evaluate the pregnancy rates in hair ewes using an Ovsynch synchronization protocol under a breeding system that combines fixed-time insemination plus a 10-day mating period as an alternative.
Materials and Methods: Through an experimental study (n=27), ewes were randomly located into one of three treatments: (1) Pre-synch...
Objective: To assess the effect of L-carnitine supplementation during in vitro oocyte maturation and in vitro culture process of bovine oocytes. Methods: L-carnitine (3.8 mM) was added to maturation medium and the effect was assessed in the quality (Experiment 1) and in the cleavage and 4-cells stage (Experiment 2). Besides, the effect of L-carniti...
Presentación El enfoque terapéutico de las entidades normales y patológicas de la reproducción es uno de los retos de la práctica veterinaria de rutina. Los estudiantes de último año de los programas de medicina veterinaria o medicina veterinaria y zootecnia, así como los profesionales dedicados a la práctica de campo, se enfrentan de manera contin...
1-Terapéutica de problemas de la pubertad y el ciclo estral frecuentes en novillas y vacas. 2019-1 Docentes: Andrés F. Mejía-Tamayo, Juan Maldonado-Estrada. Presentación La terapéutica reproductiva de la que trata este documento hace referencia a la intervención y los planes terapéuticos que debe manejar el veterinario, para la formulación de proto...
Presentación El enfoque terapéutico de las entidades normales y patológicas de la reproducción es uno de los retos de la práctica veterinaria de rutina. Los estudiantes de último año de los programas de medicina veterinaria o medicina veterinaria y zootecnia, así como los profesionales dedicados a la práctica de campo, se enfrentan de manera contin...
1-Terapéutica de problemas de la pubertad y el ciclo estral frecuentes en novillas y vacas. 2019-1 Docentes: Andrés F. Mejía-Tamayo, Juan Maldonado-Estrada. Presentación La terapéutica reproductiva de la que trata este documento hace referencia a la intervención y los planes terapéuticos que debe manejar el veterinario, para la formulación de proto...
Presentación. La farmacología reproductiva hace referencia el uso de medicamentos para el tratamiento de problemas de los ciclos reproductivos en los animales. De igual modo, paraoptimizar la reproducción a través de la biotecnología de la reproducción. El tratamiento farmacológico de los problemas de la reproducción de origen hormonal obedece al u...
Subclinical Ruminal Acidosis (SARA) and Subclinical Ketosis (SCK) are two of the most prevalent metabolic diseases of dairy cows, with impacts on reproductive performance. There is scarce literature about these diseases in dairy regions in Colombia. In 29 randomly selected herds in Pasto, Colombia, 249 dairy cows were followed weekly for two months...
New evidence suggests that glycan expression in placental cells of women with invasive disorders of pregnancy differs from that in normal pregnant women. Hypothesizing that modifications of glycan expression could account for the course of preeclampsia, we established placental villous histocultures and compared glycan expression in women with pree...
Objective: To assess the effect of L-carnitine supplementation during in vitro oocyte maturation and in vitro culture process of bovine oocytes.
Methods: L-carnitine (3.8 mM) was added to maturation medium and the effect was assessed in the quality (Experiment 1) and in the cleavage and 4-cells stage (Experiment 2). Besides, the effect of L-carniti...
Background: Maternal iron deficiency negatively affects the iron status of the newborn and could have adverse effects during childhood and adult life, particularly on cognitive and productive processes. Because few studies have related the level of iron receptor expression in the placenta with maternal and/or newborn iron status, the objective of t...
Study background: The study was designed to evaluate if the addition of Dexamethasone, IFN-g, or LPS into culture media of primary bovine Monocyte-Derived Macrophages (MDMs), could support in vitro infection with Mycobacterium avium subspecies paratuberculosis.
Methods: Primary bovine Monocyte-Derived Macrophages (MDMs) were infected in vitro with...
Opinions on ovariohysterectomy (OHE) of bitches vary depending on region and country. In this descriptive, prospective cross-sectional study, uterine tracts and ovaries exhibiting gross pathologic findings (n = 76) were collected post-surgery from a reference population of 3,600 bitches (2.11% incidence) that underwent elective OHE during September...
Polyamines are polycationic molecules that contain two or more amino groups (–NH 3⁺ ) and are present in all eukaryotic and prokaryotic cells. Polyamines are synthesized from arginine, ornithine, and proline, and from methionine as the methyl-group donor. In the traditional pathway for polyamine synthesis, arginase converts arginine into ornithine,...
Introducción: la formación en lengua inglesa se incluyó en el cambio curricular del programa de medicina veterinaria (Universidad de Antioquia), con cinco asignaturas de inglés ofrecidas por la Escuela de Idiomas de la Universidad y una asignatura tipo “content-based English” ofrecida por los docentes del Programa (12 créditos en un programa de 200...
Introducción: el nuevo currículo (versión 4) del programa de medicina veterinaria de la Universidad de Antioquia, implementado en el año 2014, requiere de un seguimiento que acompañe su desarrollo en forma permanente; de esta manera, a medida que se implemente la propuesta se establece en forma paralela un plan de mejoramiento. Para ello se acoge l...
Introducción: la integración curricular se concibe en el programa de medicina veterinaria (Universidad de Antioquia) en los siguientesaspectos: articulación del proceso de aprendizaje con la prácticaerinaria; abordaje de los problemas de salud animal de lareal y promoción de la interacción entre disciplinas; orientación estudiantes para promover co...
Introducción: la autoevaluación del programa de medicina veterinaria (Universidad de Antioquia), revelaba que los estudiantes solicitaban mayor oportunidad de trabajo práctico con las condiciones reales del ejercicio médico veterinario (praxis veterinaria); asimismo, la Universidad de Antioquia tenía espacios de aprendizaje de dicha praxis que eran...
Inclusion of English Language in the veterinary medicine curricullum, at the University of Antioquia Colombia
Background: Cell invasion and placenta development involve variations of oxygen supply that supports phenotypic changes of tumoral and trophoblast cells, including the glycocalix and membrane glycoprotein expression. Methods: JEG-3 and the extravillous trophoblastic cell line HTR8/Svneo were incubated for 12, 24 and 48 h with 150 μM CoCl2 (cobalt c...
Durante la gestación ocurren grandes cambios en el organismo de la vaca, tanto anatómicos como fisiológicos y ésta debe garantizar la seguridad física del feto y que además reciba los nutrientes y metabolitos que necesita para su desarrollo y crecimiento. Este proceso está acompañado de cambios sin precedentes en el organismo de la vaca y del funci...
El Mycobacterium avium subespecie paratuberculosis (MAP) es el agente causal de una enfermedad
granulomatosica crónica, que afecta el tracto gastrointestinal de rumiantes domesticos y salvajes,
conocida como la enfermedad de Johne o paratuberculosis. MAP es un microorganismo de crecimiento
lento en cultivo, no obstante sobrevive in vivo en célul...
Anamnesis: a six-year-old milking Holstein cow (Bos taurus taurus) was diagnosed with stage IV Johne´s
Disease (JD). Clinical and Laboratory findings: the cow suffered intermittent diarrhea during 6 months
with no response to antibiotic treatment. Consequently, the cow was subjected to euthanasia. Treatment
approach: antemortem milk and peripheral...
Mycobacterium avium subspecies paratuberculosis (MAP) is the causing agent of a chronic granulomatous disease affecting intestinal tract of domestic and wild ruminants known as Johne's disease or paratuberculosis. MAP behaves as low growing mycobacteria in vitro. However, it can survive into the phagocytic vacuole of macrophages of a susceptible in...
Preeclampsia involves an exacerbated maternal inflammatory response that suggests a possible role of innate immunity. NK cells can promote this kind of response through cytokine production and the expression of activating or inhibitory receptors. The aims of the present study were to explore cytokine production by peripheral blood mononuclear cells...
Objective. To define the presence of lung metastasis in bitches with malignant mammary neoplasms. Materials and methods. Thirty female dogs that were attended at Veterinary Hospital (University of Antioquia, Medellin, Colombia) were selected for the study. At consultation clinical variables and grade of mammary and inguinal lymph node compromise we...
Objectives. To define the presence of lung metastasis in bitches with malignant mammary neoplasms. Material and methods. Thirty female dogs that were attended at Veterinary Hospital (University of Antioquia, Medellin, Colombia) were selected for the study. At consultation clinical variables and grade of mammary and inguinal lymph node compromise we...
La adecuada transferencia placentaria de hierro es crucial para satisfacer los altos requerimientos del feto y promover su adecuado crecimiento y desarrollo intrauterino; de otra parte, contribuye a prevenir la ferropenia y la anemia, entidades altamente prevalentes en los lactantes durante los primeros dos años de edad, que se asocian con mayor mo...
La transferencia placentaria adecuada de hierro es crucial para satisfacer los altos requerimientos que el feto tiene de este mineral y para promover su crecimiento y desarrollo intrauterinos apropiados; de otra parte, contribuye a prevenir la ferropenia y la anemia, entidades muy frecuentes durante los dos primeros años de vida, y que se asocian c...
The great omentum plays an important role in protecting the peritoneal cavity from bacteria and contaminating material and providing the peritoneum with leukocytes from the omental milky spots (OMS). However, there are no reports on the existence of OMS in dogs. In this report an unusual case of asymptomatic uterine rupture (UR) is described in a 1...
The great omentum plays an important role in protecting the peritoneal cavity from bacteria and contaminating material and providing the peritoneum with leukocytes from the omental milky spots (OMS). However, there are no reports on the existence of OMS in dogs. In this report an unusual case of asymptomatic uterine rupture (UR) is described in a 1...
The aim of this study was to establish the protocol of conventional and real time PCR for amplification of Mycobacterium avium subsp. paratuberculosis (MAP) genome from bovine fecal samples, as a way to define strategies for establishing a prevention and control program in a dairy herd at the Universidad de Antioquia (Medellín, Colombia). Fecal sam...
Las anormalidades fetales afectan alrededor del 1% de los recién nacidos en todo el mundo y están asociadas con cerca del 20% de la mortalidad infantil durante el primer año de vida. El diagnóstico prenatal permite la detección temprana tanto de enfermedades del feto como de la madre, con el fin de brindar una mejor manera de afrontar la situación...
Ovulation failure is one of the most frequent causes of infertility in mares. In the present case we report a six-year-old Colombian Paso Fino maiden Mare that was attended for breeding purposes with a previous history of ovulation failure. At ultrasound (US) examination of the reproductive tract and ovaries the left ovary measured 15 x 13 cm and a...
The non-classical Major Histocompatibility Complex (MHC) molecules HLA-G and HLA-E are selectively expressed by human cytotrophoblasts during human placental development, and they are presumed to be involved in functionally modulating the immune system at the fetal-maternal interface. These non-classical MHC molecules are also expressed by JEG-3 ch...
In this work an estimated calculation of the cost of three of the most common protocols for superovulation and embryo trasnsfer in Colombia is presented. Estimate costs were defined according to commercial avalilable products at december 2005, taking into account the following sources of costs: 1) Superovulation of donor cows, 2) sinchronization of...
Desde el inicio del uso intensivo de la transferencia de embriones (TE) en Colombia, los esquemas hormonales para la inducción de la superovulación (SOV) en las donadoras y la sincronización de las receptoras (SR), se han aplicado de conformidad con los estudios realizados en otras partes del mundo. Una gran cantidad de variaciones en los esquemas...
