Juan Jose Gonzalez Plaza

Juan Jose Gonzalez Plaza
Universidad de Burgos | UBU

Ph.D. Advanced Biotechnology

About

46
Publications
9,779
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Citations
Introduction
Main interest in transcriptomics as a tool to understand biological communication (mediated by RNA). Additional interest and research lines in metagenomics and functional metagenomics, for mining key prokaryotic genes used at microbial warfare/defence, including resistance to antimicrobials.
Additional affiliations
May 2021 - September 2022
Universidad de Burgos
Position
  • PostDoc Position
August 2018 - April 2021
Czech University of Life Sciences Prague
Position
  • PostDoc Position
Description
  • I study the interaction termites-allied microbiome: how does it shape lignocellulose degradation and control of detrimental bacteria? The main studied aspects: host and microbiome transcriptome , and mining key prokaryotic genes for catabolism or warfare.
April 2017 - July 2017
Julius Kühn-Institut
Position
  • Researcher
Description
  • Short research stay (EMBO Short-Term Fellowship). The main objective of this research stay was to carry out exogenous plasmid capture assays from environmental bacteria to pathogen models. Part of WINAR project. Laboratory of Prof. Dr. Kornelia Smalla.
Education
October 2010 - May 2013
University of Malaga
Field of study
  • Plant Genomics

Publications

Publications (46)
Article
Full-text available
Plant architecture is a critical trait in fruit crops that can significantly influence yield, pruning, planting density and harvesting. Little is known about how plant architecture is genetically determined in olive, were most of the existing varieties are traditional with an architecture poorly suited for modern growing and harvesting systems. In...
Article
Full-text available
Environments polluted by direct discharges of effluents from antibiotic manufacturing are important reservoirs for antibiotic resistance genes (ARGs), which could potentially be transferred to human pathogens. However, our knowledge about the identity and diversity of ARGs in such polluted environments remains limited. We applied functional metagen...
Article
Background: The changes that are produced in the gene expression of subcutaneous adipose tissue (SAT) following a Roux-en-Y gastric bypass (RYGB) are not yet fully known. Objective: To identify the changes in the SAT gene expression of morbidly obese women with low insulin resistance (MO-low-IR) and high insulin resistance (MO-high-IR) in order to...
Article
Full-text available
High antibiotic releases from manufacturing facilities have been identified as a risk factor for antibiotic resistance development in bacterial pathogens. However, the role of antibiotic pollution in selection and transferability of antibiotic resistance genes (ARGs) is still limited. In this study, we analyzed effluents from azithromycin-synthesis...
Article
Full-text available
Communication shapes life on Earth. Transference of information has played a paramount role on the evolution of all living or extinct organisms since the appearance of life. Success or failure in this process will determine the prevalence or disappearance of a certain set of genes, the basis of Darwinian paradigm. Among different molecules used for...
Data
Supplementary Figures to the preprint "Termite diet rather than geographical origin determines the microbiome composition and functional genetic structure of nests from South American and African representatives, as revealed by a multiomics approach". 10.1101/2022.08.13.503768
Preprint
Full-text available
Termites represent one of the most important insect groups worldwide due to their key role as plant decomposers and proxy of carbon recycling in the tropical rainforest ecosystems. Besides, high relevance in research has been given to these social insects due to a prominent role as urban pests. However, one of the most fascinating aspects of termit...
Article
Full-text available
Termites are major decomposers in terrestrial ecosystems and the second most diverse lineage of social insects. The Kalotermitidae form the second-largest termite family and are distributed across tropical and subtropical ecosystems, where they typically live in small colonies confined to single wood items inhabited by individuals with no foraging...
Preprint
Full-text available
In the present study, a comparative human toxicity assessment between newly developed Mn3O4 nanoparticles with enhanced electrochemical properties (GNA35) and their precursor material (Mn3O4) was performed, employing different in vitro cellular models representing alveolar, oral and dermal exposure routes, namely the human alveolar carcinoma epithe...
Preprint
Full-text available
Termites are major decomposers of organic matter in terrestrial ecosystems and the second most diverse lineage of social insects. The Kalotermitidae, the second-largest termite family, are widely distributed across tropical and subtropical ecosystems, where they typically live in small colonies confined to single wood items inhabited by individuals...
Article
Full-text available
Simple Sequence Repeat (SSR) or microsatellite markers have become extremely useful tools in genetic identification and variability studies. To date around one hundred of these markers have been developed in the olive tree (Olea europaea L. subsp. europaea) and the majority of them are di- or tri-nucleotide. The analysis of the numerous articles re...
Article
Environments polluted with excessively high levels of antibiotics released from manufacturing sites can act as a source of transferable antibiotic resistance (AR) genes to human commensal and pathogenic bacteria. The aim of this study was to evaluate AR of bacteria isolated from the Sava river sediments (Croatia) at the discharge site of effluents...
Article
Environmental discharges of very high (mg/L) antibiotic levels from pharmaceutical production contributed to the selection, spread and persistence of antibiotic resistance. However, the effects of less antibiotic-polluted effluents (μg/L) from drug-formulation on exposed aquatic microbial communities are still scarce. Here we analyzed formulation e...
Article
Effluents from antibiotic manufacturing may contain high concentrations of antibiotics, which are the main driving force behind the selection and spread of antibiotic resistance genes in the environment. However, our knowledge about the impact of such effluent discharges on the antibiotic resistome and bacterial communities is still limited. To gai...
Data
The NJ dendrogram including all cultivated plant material analyzed in the study.
Article
Full-text available
Germplasm collections are basic tools for conservation, characterization, and efficient use of olive genetic resources. The identification of the olive cultivars maintained in the collections is an important ongoing task which has been performed by both, morphological and molecular markers. In the present study, based on the sequencing results of p...
Article
Intercellular communication is a widespread phenomenon in all domains of Life. Bacteria have developed many ways of communicating with one another and with other species, either prokaryotic or eukaryotic. RNA has been a key molecule since beginning of Life on Earth, and is one of the carriers of information. Given the current antibiotic crisis, und...
Article
Effluents from pharmaceutical industries are recognized as significant contributors to aquatic pollution with antibiotics. Although such pollution has been mostly reported in Asia, knowledge on industrial discharges in other regions of the world, including Europe, and on the effects associated with such exposures is still limited. Thus, we performe...
Article
Full-text available
Viruses have been for long polemic biological particles which stand in the twilight of being living entities or not. As their genome is reduced, they rely on the metabolic machinery of their host in order to replicate and be able to continue with their infection process. The understanding of their metabolic requirements is thus of paramount impo...
Article
Full-text available
Fever or pyrexia is a process where normal body temperature is raised over homeostasis conditions. Although many effects of fever over the immune system have been known for a long time, it has not been until recent studies when these effects have been evaluated in several infection processes. Results have been promising, as they have reported ne...
Article
Full-text available
Among all new emerging RNA species, microRNAs (miRNAs) have attracted the interest of the scientific community due to their implications as biomarkers of prognostic value, disease progression, or diagnosis, because of defining features as robust association with the disease, or stable presence in easily accessible human biofluids. This field of res...
Article
Full-text available
Pseudomonas syringae is a bacterial plant pathogen that can lead to heavy losses in crop production. This bacteria is a very good model to study the infection processes, as it can cause disease in Arabidopsis thaliana, a well-studied plant model. This text presents an overview of the bacterial pathogenesis from a molecular biology perspective, and...
Article
Full-text available
For centuries olive tree is an important crop in many Mediterranean countries because it provides appreciated oil with healthy properties. The lack of genomic tools, such as molecular markers or sequence information, has hindered the development of new cultivars adapted to the challenges that this species faces due to the change in modern cultivati...
Article
Full-text available
Oesophageal Squamous Cell Carcinoma (ESCC) is one of the two main subtypes of oesophageal cancer, affecting mainly populations in Asia. Though there have been great efforts to develop methods for a better prognosis, there is still a limitation in the staging of this affection. As a result, ESCC is detected at advances stages, when the interventions...
Article
Background The changes in the transcriptomic profiling of subcutaneous adipose tissue (SAT) when weight loss stabilizes after a Roux-en-Y gastric bypass (RYGB) are still largely unknown. Objectives To investigate the changes produced in SAT gene expression of morbidly obese women when their weight loss stabilizes 2 years after RYGB. Methods SA...
Article
In the present work, a set of eight new hexa-nucleotide simple sequence repeats (SSRs) is reported in olive (Olea europaea L). These SSRs loci were generated on the basis of expressed sequence tag (EST) sequences in the frame of an olive genomic project. The markers showed a high level of polymorphism when tested on a set of cultivars used as genit...
Thesis
Introducción La arquitectura en plantas se define como la organización espacial de las mismas, y ha sido el criterio principal para la clasificación taxonómica de plantas durante mucho tiempo. De forma adicional es importante como carácter agronómico, ya que influye en la idoneidad de una planta para su cultivo, incluyendo el proceso de cosechado y...
Article
Full-text available
Olive breeding programmes are focused on selecting for traits as short juvenile period, plant architecture suited for mechanical harvest, or oil characteristics, including fatty acid composition, phenolic, and volatile compounds to suit new markets. Understanding the molecular basis of these characteristics and improving the efficiency of such bree...
Article
Full-text available
Several reports have recently contributed to determine the effector inventory of the sequenced strain Pseudomonas syringae pv. phaseolicola (Pph) 1448a. However, the contribution to virulence of most of these effectors remains to be established. Genetic analysis of the contribution to virulence of individual P. syringae effectors has been tradition...
Data
Cancelled-out index (COI) analysis of Pph 1448a effectors HopAB1 and HopQ1. The double mutants strains were co-infiltrated with each single mutant strain and the corresponding COI was determined at either at 7 dpi. Each relevant CIs is included in the figure (grey) for comparison purposes. Each COI corresponds to the mean of at least three independ...
Data
Theoretical representation of COI analysis of the interaction between two hypothetical genes, a and b. A. CI is defined as the mutant-to-wt output ratio divided by the mutant-to-wt input ratio. COI is defined as the double mutant-to-single mutant output ratio divided by the double mutant-to-single mutant input ratio. B. Determination and analysis o...
Data
Sequence analysis of effectors HopAB1 and HopI1. A. Comparison of HopAB1Pph1448a and AvrPtoBPtoDC3000 amino acid sequences. Identical amino acids are highlighted in blue. Sequences display 55% overall identity, while the C-terminal region predicted to comprise the E3 ligase domain (Pfam ID: PF09046, underlined), displays 77% identity. Position of c...
Data
Numerical values corresponding for the CIs and COIs displayed in figure 3, 4, 5, 6, 7 and S1. (DOC)
Article
Full-text available
The effect of salinity on plants of Colocasia esculenta (taro) has been investigated through the determination of polyamines content and peroxidase activity. Micropropagated plants were subbmitted to three treatments : 0, 50, and 100 mmol L-1 NaCl, for 30 days, and the putrescine, spermidine and spermine levels, peroxidase acitivity and Na conce...

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Projects

Projects (2)
Archived project
An exploratory project aimed to mine the genetic functional potential of microbial communities associated to termites.
Archived project
This project is directed by Dr. Nikolina Udiković Kolić. The increasing prevalence of antibiotic resistant bacteria is currently one of the most serious health threats. There is growing evidence that continuous environmental discharge of antibiotics and heavy metals contributes to this issue. The selection pressure imposed by these pollutants has promoted the development and spread of antibiotic resistance among commensal and pathogenic bacteria. Although pharmaceutical waste is recognized as the most important point source of these pollutants in the receiving aquatic environment, its impact on the composition and antibiotic resistance profile of exposed microbial communities is not known. To fill this important research gap, we propose to take an interdisciplinary approach focusing on freshwater sediments impacted by wastewaters of two local pharmaceutical industries. We will assess the prevalence of antibiotics and heavy metals in these sediments and identify potential hot spots for resistance evolution. Antibiotic resistance genes from hot spots and reference sites will be discovered using functional metagenomics. This will lay the groundwork for a quantitative study that will establish spatio-temporal relationships between industrial discharge and the occurrence of antibiotic resistance. This, in combination with direct capturing of resistance plasmids from sediment bacteria to a model pathogens will be critical to draw conclusions about the spread of resistance genes among bacteria. Complete sequencing of transferable plasmids will furthermore assist in identifying novel plasmids that carry clustered antibiotic and heavy metal resistance gene loci. Finally, the impact of discharge on dynamics of sediment community composition will be analyzed by Illumina sequencing of 16S rRNA genes. We believe that the obtained knowledge will have vital implications for the development of effective management strategies to reduce the spread of antibiotics and antibiotic resistance determinants via environmental pathways.