Juan Fernández García

Juan Fernández García
Vlaams Instituut voor Biotechnologie | VIB · VIB-KU Leuven Center for Cancer Biology

PhD

About

24
Publications
6,955
Reads
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763
Citations
Additional affiliations
April 2016 - present
Vlaams Instituut voor Biotechnologie
Position
  • PostDoc Position
May 2015 - February 2016
Yale University
Position
  • PostDoc Position

Publications

Publications (24)
Article
Full-text available
Cancer metastasis requires the transient activation of cellular programs enabling dissemination and seeding in distant organs1. Genetic, transcriptional and translational heterogeneity contributes to this dynamic process2,3. Metabolic heterogeneity has also been observed4, yet its role in cancer progression is less explored. Here we find that the l...
Article
Full-text available
Macrophages are often prominently present in the tumor microenvironment, where distinct macrophage populations can differentially affect tumor progression. Although metabolism influences macrophage function, studies on the metabolic characteristics of ex vivo tumor-associated macrophage (TAM) subsets are rather limited. Using transcriptomic and met...
Preprint
Cytotoxic T cells dynamically rewire their metabolism during the course of an immune response. While T cell metabolism has been extensively studied at phenotypic endpoints of activation and differentiation, the underlying dynamics remain largely elusive. Here, we leverage on single-cell RNA-sequencing (scRNA-seq) measurements of in vitro activated...
Article
Full-text available
Hepatic fat accumulation is associated with diabetes and hepatocellular carcinoma (HCC). Here we characterize the metabolic response that high fat availability elicits in livers prior to disease development. After a short term on a high fat diet, otherwise healthy mice showed elevated hepatic glucose uptake and increased glucose contribution to ser...
Preprint
Cancer metastasis requires the transient activation of cellular programs enabling dissemination and seeding in distant organs. Genetic, transcriptional and translational intra-tumor heterogeneity contributes to this dynamic process. Beyond this, metabolic intra-tumor heterogeneity has also been observed, yet its role for cancer progression remains...
Article
Full-text available
The risk of cancer and associated mortality increases substantially in humans from the age of 65 years onwards1–6. Nonetheless, our understanding of the complex relationship between age and cancer is still in its infancy2,3,7,8. For decades, this link has largely been attributed to increased exposure time to mutagens in older individuals. However,...
Preprint
Full-text available
From age 65 onwards, the risk of cancer incidence and associated mortality is substantially higher1-3. Nonetheless, our understanding of the complex relationship between age and cancer is still in its infancy4. For decades, the link has largely been attributed to increased exposure time to mutagens in older individuals. However, this view does not...
Article
Full-text available
Depleting regulatory T cells (Treg cells) to counteract immunosuppressive features of the tumor microenvironment (TME) is an attractive strategy for cancer treatment; however, autoimmunity due to systemic impairment of their suppressive function limits its therapeutic potential. Elucidating approaches that specifically disrupt intratumoral Treg cel...
Article
Full-text available
Metabolism is at the cornerstone of all cellular functions and mounting evidence of its deregulation in different diseases emphasizes the importance of a comprehensive understanding of metabolic regulation at the whole-organism level. Stable-isotope measurements are a powerful tool for probing cellular metabolism and, as a result, are increasingly...
Chapter
Full-text available
Metastasis formation is the leading cause of death in cancer patients. It has recently emerged that cancer cells adapt their metabolism to successfully transition through the metastatic cascade. Consequently, measuring and analyzing the in vivo metabolism of metastases has the potential to reveal novel treatment strategies to prevent metastasis for...
Article
Electrospraying (ES) dissolved viral particles, followed by charge reduction and size analysis with a differential mobility analyzer (DMA), offers a flexible size-analysis tool for small particles in solution. The technique relies on pioneering work by Kaufman and colleagues, commercialized by TSI, and often referred to as GEMMA. However, viral stu...
Article
Full-text available
Most tumours have an aberrantly activated lipid metabolism1,2 that enables them to synthesize, elongate and desaturate fatty acids to support proliferation. However, only particular subsets of cancer cells are sensitive to approaches that target fatty acid metabolism and, in particular, fatty acid desaturation³. This suggests that many cancer cells...
Chapter
Full-text available
Immune cell function is tightly regulated by cellular metabolism, which in turn is strongly linked to the nutrient availability in the microenvironment surrounding the cells. This link is critical for effector CD8⁺ T cells which, after activation, must migrate from nutrient-rich environments into nutrient-scarce regions such as the tumor microenvir...
Article
Full-text available
A pending issue in linking ion mobility measurements to ion structures is that the collision cross section (CCS, the measured structural parameter in ion mobility spectrometry) of an ion is strongly dependent upon the manner which gas molecules effectively impinge and are reemitted from ion surfaces (when modeling ions as fixed structures). To dire...
Article
Prior work with electrosprays in vacuum of mixtures of ionic liquids (ILs) and the moderately high boiling point (Tb) solvents formamide (FM) and propylene carbonate (PC) (Tb of 210 and 241 °C) has shown that the charged drops produced have reasonably narrow charge/mass distributions, controllable over a wide mass/charge range. This enables their u...
Article
The electrical mobilities of hundreds of mass-selected, multiply charged nanodrops (2-6 nm in diameter) of the ionic liquid EMI-BF4 have been measured in air and CO2 at temperatures, T, ranging from 20 to 100 °C, extending previous studies, based on EMI-N(CN)2 nanodrops in air at 20 °C, to other temperatures and drift gases. The known compressibili...
Article
The electrical mobilities of multiply-charged nanodrops of the ionic liquid 1-ethyl, 3-methylimidazolium dicyanamide (EMI-N[CN]2) were accurately measured in air at 20 °C for mass-selected clusters of composition [EMI-N[CN]2] n [EMI(+)] z , with 2 ≤ n ≤ 369 and 1 ≤ z ≤ 10. We confirm prior reports that the mobility Z of a globular ion of mass m is...
Article
The charge distribution of polystyrene nanoparticles electrosprayed from l-methyl-2-pyrrolidone (NMP) dimethylammonium formate (10/1 vol.) is studied by a continuous tandem differential mobility analyzer (TDMA) technique. Two DMAs operated in series as narrow band mobility filters are scanned in a quasi-continuous fashion providing highly informati...
Article
The mass spectrometric (MS) complexity associated with the quasi-continuous distribution of mass and charge (m, z) of electrosprayed industrial polymers may be moderated by use of ion mobility spectrometry (IMS) and MS in series. However, when the high charge levels typical of polar polymers stretch the gas phase ions into linear configurations, th...
Article
The chargedistributionofpolystyrenenanoparticleselectrosprayedfroml-methyl-2- pyrrolidone(NMP)dimethylammoniumformate(10/1vol)isstudiedbya continuous tandem differentialmobilityanalyzer(CTDMA)technique.TwoDMAsoperatedinseries as narrowbandmobilityfiltersarescannedinaquasi-continuousfashionproviding highlyinformativetwo-dimensional(2D)spectra.Thefir...
Article
Full-text available
The validity of the Stokes-Millikan equation is examined in light of mass and mobility measurements of clusters of the ionic liquid 1-ethyl-3-methyl-imidazolium tetrafluoroborate (EMI-BF4) in ambient air. The mobility diameter dZ based on the measured mobility and the Stokes-Millikan law is compared with the volume diameter dv, which generalizes th...

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