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Publications (297)
Primers used in the qPCR analysis study.
(XLSX)
Statistical analysis comparing ion relative abundances of Grazed vs. Control Lessonia spicata samples, collected after 6, 12, 24 and 48h (n = 3, except for Control-48h n = 2).
Peak assignation, ion identification and relative abundance calculation were processed by XCMS analysis. Each ion is identified by a unique ID (MxTy) indicating its mass (Mx,...
List of 122 putative differentially expressed unigenes under grazing pressure in Laminaria digitata, identified by 454 sequencing and EST analysis.
(XLSX)
Detailed list of identified ions by LC/MS analysis and their relative abundance from Laminaria digitata samples, under control and grazed conditions, collected after 6, 12, 24 and 48h (n = 4, except for Grazed-48h n = 2).
Peak assignation, ion identification and relative abundance calculation were processed by XCMS analysis. Each ion is identified...
Detailed list of identified ions by LC/MS analysis and their relative abundance from Lessonia spicata samples, under control and grazed conditions, collected after 6, 12, 24 and 48h (n = 3, except for Control-48h n = 2).
Peak assignation, ion identification and relative abundance calculation were processed by XCMS analysis. Each ion is identified b...
List of 132 putative differentially expressed unigenes under grazing pressure in Lessonia spicata, identified by 454 sequencing and EST analysis.
(XLSX)
Representative pictures of kelps with their respective specialist herbivores.
(A) Laminaria digitata and Patella pellucida Linnaeus. (B) Lessonia spicata and Scurria scurra, on Algal tissues showing typical grazing damages are presented. Photos by LC, FT, CF, SF and CL.
(TIF)
Fold-Change and statistical analysis comparing qPCR transcript levels of selected unigenes between grazed and control individuals of L. digitata.
(XLSX)
Statistical analysis comparing ion relative abundances of Grazed vs. Control Laminaria digitata samples, collected after 6, 12, 24 and 48h (n = 4, except for Grazed-48h n = 2).
Peak assignation, ion identification and relative abundance calculation were processed by XCMS analysis. Each ion is identified by a unique ID (MxTy) indicating its mass (Mx...
Fold-Change and statistical analysis comparing amino acid, free fatty acids and oxylipin relative abundances of Grazed vs. Control Lessonia spicata samples, collected after 6, 12, 24 and 48h.
For each metabolite and sampling time, fold-change was calculated by dividing the average of ion relative abundance in Grazed samples by that from Control sam...
Fold-Change and statistical analysis comparing qPCR transcript levels of selected unigenes between grazed and control individuals of L. spicata.
(XLSX)
Lessonia spicata is a key dominant species along the Pacific coast of South America, providing a habitat for many organisms. However, this role can be affected by abiotic stress, such as metals. To counteract the toxic effect, L. spicata, among other seaweeds, releases exudates that bind metals. In this study, tolerances to copper of organisms rela...
Main conclusion:
The hormone ABA regulates the oxidative stress state under desiccation in seaweed species; an environmental condition generated during daily tidal changes. Desiccation is one of the most important factors that determine the distribution pattern of intertidal seaweeds. Among most tolerant seaweed is Pyropia orbicularis, which colon...
Associated microbiota play crucial roles in health and disease of higher organisms. For macroalgae, some associated bacteria exert beneficial effects on nutrition, morphogenesis and growth. However, current knowledge on macroalgae-microbiota interactions is mostly based on studies on green and red seaweeds. In this study, we report that when cultur...
Extreme reduction in cellular water content leads to desiccation, which, if persistent, affects the physiology of organisms, mainly through oxidative stress. Some organisms are highly tolerant to desiccation, including resurrection plants and certain intertidal seaweeds. One such species is Pyropia orbicularis, a rhodophycean that colonizes upper i...
Point 1:
Management of crops, commercialized or protected species, plagues or life-cycle evolution are subjects requiring comparisons among different demographic strategies. The simpler methods fail in relating changes in vital rates with changes in population viability whereas more complex methods lack accuracy by neglecting interactions among vi...
The link between life history traits and mating systems in diploid organisms has been extensively addressed in the literature, whereas the degree of selfing and/or inbreeding in natural populations of haploid-diploid organisms, in which haploid gametophytes alternate with diploid sporophytes, has been rarely measured. Dioecy has often been used as...
Adaptation to marginal habitats at species range-limits has often been associated with parthenogenetic reproduction in terrestrial animals and plants. Laboratory observations have shown that brown algae exhibit a high propensity for parthenogenesis by various mechanisms. The kelp Laminaria digitata is an important component of the ecosystem in Nort...
Background
Brown algae are sessile macro-organisms of great ecological relevance in coastal ecosystems. They evolved independently from land plants and other multicellular lineages, and therefore hold several original ontogenic and metabolic features. Most brown algae grow along the coastal zone where they face frequent environmental changes, inclu...
In rocky shores, desiccation is triggered by daily tide changes, and experimental evidence
suggests that local distribution of algal species across the intertidal rocky zone is related to their capacity to
tolerate desiccation. In this context, the permanence of Pyropia columbina in the high intertidal rocky zone is
explained by its exceptional...
Tolerance to air exposure should be an important
feature in determining the geographic distribution of
seaweeds. Two sibling kelp species with contrasting latitudinal
distributions were selected to test the relationship
between their distribution and air exposure tolerance:
Lessonia berteroana distributed between 18° and 30°S and
Lessonia spicata,...
Among thiol-dependent peroxidases (TDPs) peroxiredoxins (PRXs) standout, since they are enzymes capable of reducing hydrogen peroxide, alkylhydroperoxides and peroxynitrite, and have been detected in a proteomic study of the copper-tolerant species Scytosiphon gracilis. In order to determine the importance of these enzymes in copper-stress toleranc...
In order to better understand the alternation of generations that characterizes haploid–diploid life cycles, we assessed the existence of ecological differences between the two phases (haploid gametophyte and diploid tetraspor-ophyte) in Gracilaria chilensis, a rhodophyte with a typical Polysiphonia-type life cycle. We investigated the effect of li...
A major determinant of the geographic distribution of a species is expected to be its physiological response to changing abiotic variables over its range. The range of a species often corresponds to the geographic extent of temperature regimes the organism can physiologically tolerate. Many species have very distinct life history stages that may ex...
The kelp Lessonia nigrescens Bory is the most ecologically and economically important seaweed in rocky intertidal and shallow subtidal habitats along the temperate Pacific South American coasts. Recent molecular studies suggest the existence of two lineages, one (northern lineage) from 17 degrees S to 30 degrees S and a second (central lineage) fro...
Different propagation techniques for cultivation of vegetative Gigartina skottsbergii fronds were tested using a system of suspended ropes, to which inoculants were attached. Our results showed that triangular fragments obtained from the circular G. skottsbergii thalli produced harvestable frond of 800 cm 2 after 8 months. In contrast, inoculants o...
Copper has a dual role for organisms, both as micronutrient and toxic element. Copper mining activities have an enormous ecological impact because of the extraction process and the consequent release of copper-containing waste materials to the environment. In northern Chile, mainly in the Chañaral coastal area, this phenomenon is clearly evident. T...
The red alga Gracilaria chilensis is commercially farmed for the production of agar hydrocolloids, but some susceptible algae in farms suffer from intense epiphyte growth. We investigated the induced chemical defense response of G. chilensis against epiphytes and demonstrated that an extract of an epiphyte-challenged alga can trigger a defense resp...
The effects of desiccation on the early development stages of Mazzaella laminarioides, Scytosiphon lomentaria and Lessonia nigrescens, algal species with different patterns of distribution across the intertidal zone, were examined in the laboratory. In addition, the protective effect against desiccation was evaluated using algal extracts, including...
Little is known about variation of sex ratio, the proportion of males to females, in natural populations of seaweed, though it is a major determinant of the mating system. The observation of sexual chromosomes in kelps suggested that sex is partly genetically determined. However, it is probably not purely genetic since the sex ratio can be modified...
Culture studies of Gomontia polyrhiza from Canada and Denmark verify the presence of this species in the two countries and confirm the life history. Chlorojackia pachyclados gen. et sp. nov. has some features in common with Gomontia, but the presence of a parenchymatous thallus, vegetative cells with lobed chloroplast, different pattern of germinat...
Tolerance to air exposure should be an important feature in determining the geographic distribution of seaweeds. Two sibling kelp species with contrasting latitudinal distributions were selected to test the relationship between their distribution and air exposure tolerance: Lessonia berteroana distributed between 18°and 30°S and Lessonia spicata, w...
Unravelling the mechanisms underlying desiccation tolerance is crucial in order to understand the position of algal species in the intertidal zone. The alga Porphyra columbina lives in the uppermost part of the rocky intertidal zones around the world and was selected as a model for this study. Naturally desiccated plants were collected during low t...
Combined phylogenetic, physiological, and biochemical approaches revealed that differences in defense-related responses among 17 species belonging to the Gracilariaceae were consistent with their evolutionary history. An oxidative burst response resulting from activation of NADPH oxidase was always observed in two of the subgenera of Gracilaria sen...
• The origin of the Ectocarpus strain used for genome sequencing (the 'genome strain') was Peru, where no Ectocarpus had been reported previously. To study the genetic diversity in the region and to increase the number of individuals from this area available for genetic experiments, 119 new Ectocarpus strains were isolated from eight localities alo...
In order to identify genes/proteins involved in copper tolerance, the marine alga Ulva compressa was cultivated with 10 μM copper for 3 days. The activities of antioxidant enzymes ascorbate peroxidase (AP), peroxiredoxin (PRX), thioredoxin (TRX), and glutathione-S-transferase (GST) and the level of lipoperoxides were determined in the alga cultivat...
Ectocarpus siliculosus is a cosmopolitan brown alga with capacity to thrive in copper enriched environments. Analysis of copper toxicity was conducted in two strains of E. siliculosus isolated from (i) an uncontaminated coast in southern Peru (Es32) and (ii) a copper polluted rocky beach in northern Chile (Es524). Es32 was more sensitive than Es524...
In order to analyse copper-induced calcium release and (reactive oxygen species) ROS accumulation and their role in antioxidant and defense enzymes activation, the marine alga Ulva compressa was exposed to 10 µM copper for 7 d. The level of calcium, extracellular hydrogen peroxide (eHP), intracellular hydrogen peroxide (iHP) and superoxide anions (...
This study reports on the factors involved in regulating the composition and structure of bacterial communities epiphytic on intertidal macroalgae, exploring their temporal variability and the role of copper pollution. Culture-independent, molecular approaches were chosen for this purpose and three host species were used as models: the ephemeral Ul...
In the last decades, the worldwide increase in copper wastes release by industrial activities like mining has driven environmental metal contents to toxic levels. For this reason, the study of the biological copper-resistance mechanisms in natural environments is important. Therefore, an appropriate molecular tool for the detection and tracking of...
As part of a long-term study on the biology and
ecology of the intertidal kelp Lessonia nigrescens
Bory, we report on the occurrence of gall development
on this alga, identified the possible causal
agent, and assessed the extent of the phenomenon
in two wild stands of the host. Our results showed
that galls affecting natural populations of L. nigre...
Copper complexation in marine systems is mainly controlled by organic matter, partially produced by micro- and macroalgae that release exudates with the capacity to bind metals. This feature is important as it influences bioavailability, bioaccumulation, toxicity, and transport of copper through biological membranes. The release of Cu-complexing li...
A proteomic analysis combining peptide de novo sequencing and BLAST analysis was used to identify novel proteins involved in copper tolerance in the marine alga Scytosiphon gracilis (Phaeophyceae). Algal material was cultivated in seawater without copper (control) or supplemented with 100 microg L(-1) for 4 days, and protein extracts were separated...
Phylogeographic studies are lacking in the Southern Hemisphere, and in particular in the south-eastern Pacific. To infer the possible scenario for the debated biogeographic transition zone located at 30-33 degrees S along the Chilean coast, we investigated whether there is a concordance between the phylogeographic pattern and the biogeographic tran...
In order to help explain the absence of the brown kelp Lessonia nigrescens from a coastal environment chronically enriched with copper, we characterized the biochemical responses induced by copper stress in this kelp and compared them with those displayed by the copper tolerant brown alga Scytosiphon lomentaria. These algae were cultivated with inc...