Juan Camilo Moreno Beltran

Juan Camilo Moreno Beltran
King Abdullah University of Science and Technology | KAUST · Center for Desert Agriculture​

Doctor of Philosophy

About

33
Publications
10,817
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546
Citations
Introduction
My research interest is the carotenoid pathway and more specifically how expression/accumulation of carotenoids can influence plant yield. Currently I am leading two projects with the goal of demonstrating that by manipulation of carotenogenic gene expression it is possible to influence plant yield. In addition, I am also working on b-cyclocitral and dhA signaling molecules in tobacco and Arabidopsis, applying new techniques to find receptors and interactors.
Additional affiliations
August 2016 - July 2018
Max Planck Institute of Molecular Plant Physiology
Position
  • PostDoc Position
October 2012 - July 2016
Max Planck Institute of Molecular Plant Physiology
Position
  • PhD Student
March 2010 - June 2010
University of Chile
Position
  • Teaching asistant
Description
  • Organization of the practical courses. Correction of some tests. Coordination of some tasks of the course with approximately 25 students.
Education
October 2012 - July 2016
Max Planck Institute of Molecular Plant Physiology
Field of study
  • Molecular Plant Physiology/Study of Clp protease in tobacco
March 2004 - December 2009
University of Chile
Field of study
  • Molecular Biotechnology Engineering

Publications

Publications (33)
Article
Full-text available
Small molecules not only represent cellular building blocks and metabolic intermediates, but also regulatory ligands and signaling molecules that interact with proteins. Although these interactions affect cellular metabolism, growth, and development, they have been largely understudied. Herein, we describe a method, dubbed PROtein-Metabolite Intera...
Article
Full-text available
The Clp protease in the chloroplasts of plant cells is a large complex composed of at least 13 nucleus-encoded subunits and one plastid-encoded subunit which are arranged in several ring-like structures. The proteolytic P-ring and the structurally similar R-ring form the core complex that contains the proteolytic chamber. Chaperones of the HSP100 f...
Article
Full-text available
Carotenoids, chlorophylls and gibberellins are derived from the common precursor geranylgeranyl diphosphate (GGPP). One of the enzymes in carotenoid biosynthesis is lycopene β-cyclase (LCYB) that catalyzes the conversion of lycopene into β-carotene. In carrot, Dclcyb1 is essential for carotenoid synthesis in the whole plant. Here we show that when...
Article
Full-text available
Protein degradation in chloroplasts is carried out by a set of proteases that eliminate misfolded, damaged, or superfluous proteins. The ATP-dependent caseinolytic protease (Clp) is the most complex protease in plastids and has been implicated mainly in stromal protein degradation. In contrast, FtsH, a thylakoid membrane-associated metallo-protease...
Article
Full-text available
Plant carotenoids are synthesized and accumulated in plastids through a highly regulated pathway. Lycopene β-cyclase (LCYB) is a key enzyme involved directly in the synthesis of α-carotene and β-carotene through the cyclization of lycopene. Carotenoids are produced in both carrot (Daucus carota) leaves and reserve roots, and high amounts of α-carot...
Preprint
The enzyme DWARF27 (D27) catalyzes the reversible isomerization of all- trans - into 9- cis -β-carotene, initiating strigolactone (SL) biosynthesis. Genomes of higher plants encode two D27-homologs, D27-like1 and -like2, with unknown functions. Here, we investigated the enzymatic activity and biological function of the Arabidopsis D27-like1. In vit...
Chapter
Carotenoids represent a large class of isoprenoid pigments found in nature. These compounds are synthesized in non-photosynthetic and photosynthetic organisms and have a multitude of functions related to photosynthesis, protection against biotic and abiotic stress, and signaling in development and with other organisms. Thus, manipulation of caroten...
Preprint
The role of cyclic adenosine monophosphate (3’,5’-cAMP) in plants is not well understood, and here, we report a novel role of 3’,5’-cAMP in regulating the actin cytoskeleton. The 3’,5’-cAMP treatment affects the thermal stability of 51 proteins, including a vegetative actin isoform, ACTIN2. Consistent with the above results, the increase in 3’,5’-c...
Article
Full-text available
The role of the RNA degradation product 2’,3’-cyclic adenosine monophosphate (2’,3’-cAMP) is poorly understood. Recent studies have identified 2’,3’-cAMP in plant material and determined its role in stress signaling. The level of 2’,3’-cAMP increases upon wounding, in the dark, and under heat, and 2’,3’-cAMP binding to an RNA-binding protein, Rbp47...
Article
Full-text available
Improving yield, nutritional value and tolerance to abiotic stress are major targets of current breeding and biotechnological approaches that aim at increasing crop production and ensuring food security. Metabolic engineering of carotenoids, the precursor of vitamin-A and plant hormones that regulate plant growth and response to adverse growth cond...
Article
Full-text available
Abscisic acid (ABA) is an important carotenoid-derived phytohormone that plays essential roles in plant response to biotic and abiotic stresses as well as in various physiological and developmental processes. In Arabidopsis, ABA biosynthesis starts with the epoxidation of zeaxanthin by the ABA DEFICIENT 1 (ABA1) enzyme, leading to epoxycarotenoids,...
Chapter
Apocarotenoids (APOs) are a class of carotenoid oxidation products with high structural and functional diversity. Apart from serving as precursors of phytohormones, fungal pheromones and vitamin A, several APOs act as signaling molecules involved in stress response and growth as regulators in plants. To comprehensively profile plant APOs, we establ...
Preprint
Full-text available
For many years, 2′,3′-cyclic adenosine monophosphate (2′,3′-cAMP), a positional isomer of the second messenger 3′,5′-cAMP, has not received enough attention. Recent studies have reported that 2′,3′-cAMP exists in plants and might be involved in stress signaling because 1) its level increases upon wounding and 2) it was shown to participate in stres...
Article
Full-text available
How organisms integrate metabolism with the external environment is a central question in biology. Here, we describe a novel regulatory small molecule, a proteogenic dipeptide Tyr-Asp, which improves plant tolerance to oxidative stress by directly interfering with glucose metabolism. Specifically, Tyr-Asp inhibits the activity of a key glycolytic e...
Preprint
Full-text available
Improving yield, nutritional value and tolerance to abiotic stress are major targets of current breeding and biotechnological approaches that aim at increasing crop production and ensuring food security. Metabolic engineering of carotenoids, the precursor of Vitamin-A and plant hormones that regulate plant growth and response to adverse growth cond...
Article
Full-text available
Protein-metabolite interactions are of crucial importance for all cellular processes but remain understudied. Here, we applied a biochemical approach named PROMIS, to address the complexity of the protein-small molecule interactome in the model yeast Saccharomyces cerevisiae. By doing so, we provide a unique dataset, which can be queried for intera...
Article
Full-text available
Recently, we published a set of tobacco lines expressing the Daucus carota (carrot) DcLCYB1 gene with accelerated development, increased carotenoid content, photosynthetic efficiency, and yield. Because of this development, DcLCYB1 expression might be of general interest in crop species as a strategy to accelerate development and increase biomass p...
Article
Full-text available
Carotenoids are important isoprenoids produced in the plastids of photosynthetic organisms that play key roles in photoprotection and antioxidative processes. β-carotene is generated from lycopene by the lycopene β-cyclase (LCYB). Previously, we demonstrated that the introduction of the Daucus carota (carrot) DcLCYB1 gene into tobacco (cultivar Xan...
Article
Full-text available
Carotenoids are isoprenoid compounds synthesized by all photosynthetic and some non‐photosynthetic organisms. They are essential for photosynthesis and contribute to many other aspects of a plant's life. The oxidative breakdown of carotenoids gives rise to the formation of a diverse family of essential metabolites called apocarotenoids. This metabo...
Article
Full-text available
Plants exhibit different physiological and molecular responses to adverse changes in their environment. One such molecular response is the sequestration of proteins, RNAs, and metabolites into cytoplasmic bodies called stress granules (cSGs). Here we report that, in addition to cSGs, heat stress also induces the formation of SG-like foci (cGs) in t...
Article
Because carotenoids act as accessory pigments in photosynthesis, play a key photoprotective role, and are of major nutritional importance, carotenogenesis has been a target for crop improvement. Although carotenoids are important precursors of phytohormones, previous genetic manipulations reported little if any effects on biomass production and pla...
Article
Carotenoids are plastid isoprenoid pigments that play critical roles in light harvesting, photoprotection, and phytohormone biosynthesis. They are also vitamin-A precursors and antioxidant molecules important for human nutrition. Apples (e.g. Malus x domestica Borkh), one of the most widely consumed fruits with high nutrient levels, have a very low...
Article
Full-text available
Stress granules (SGs) are evolutionary conserved aggregates of proteins and untranslated mRNAs formed in response to stress. Despite their importance for stress adaptation, no complete proteome composition has been reported for plant SGs. Herein, we addressed the existing gap. Importantly, we also provide evidence for metabolite sequestration withi...
Article
Full-text available
Chloroplasts, the green plastid-type present in all photosynthetic organisms, are the physical place where photosynthesis and many other metabolic pathways occur. Chloroplasts are essential for plants, not only by performing photosynthesis but also due to the production of important compounds comprising a great variety of secondary metabolites, lip...
Article
Full-text available
2',3'-cyclic adenosine monophosphate (2',3'-cAMP), an intriguing small molecule, is conserved among different kingdoms. This small molecule is presumably produced during RNA degradation, with increased cellular levels especially upon stress conditions. We observed its presence in protein complexes isolated from native Arabidopsis lysate, suggesting...
Thesis
Full-text available
Protein degradation in the chloroplast is carried out by a set of proteases, which are in charge of degrading misfolded, damaged or superfluous proteins. More than fifteen types of proteases are found in plastids, with Clp, FtsH, Lon and Deg being the most important (major) proteases. The ATPdependent caseinolytic protease (Clp) is the most complex...
Chapter
Carotenoids are precursors of carotenoid derived molecules termed apocarotenoids, which include isoprenoids with important functions in plant-environment interactions such as the attraction of pollinators and the defense against pathogens and herbivores. Apocarotenoids also include volatile aromatic compounds that act as repellents, chemoattractant...
Chapter
Full-text available
This comprehensive, edited book explores carotenoids and their important functional roles in yeast, bacteria and plants and a profound exposition on the structures of carotenoid molecules, focusing in the first of three parts on the biosynthesis of carotenoids. The regulation of carotenoid biosynthesis in photosynthesis as well as in plant, fruits,...
Thesis
Full-text available
Carotenoids are lipidic isoprenoid compounds synthesized by all photosynthetic organisms and also by bacteria and yeasts. In plants, these compounds are synthesized in plastids and they are essentials in these organisms due to their role in proccesses like photosynthesis, photo-protection and hormone synthesis. In animals, they act as antioxidant m...
Article
Full-text available
Carrot is an important nutritional crop due to the high levels of pro-vitamin A carotenoids (β-carotene and, to a lower extent, α-carotene) that accumulate in its storage root during secondary growth. In this work we show that in carrots, contrary to that reported for aerial organs of other plant species, light has a profound effect on root develop...
Thesis
Full-text available
The carotenoids are very important isoprenoid compounds naturally sinthesyzed by plants, algae, fungi, some yeasts and bacteria. These pigments are essential both in plants and animals. In animals, they act as antioxidants and vitamin A precursors. In plants, they are synthesized in vegetal cells plastids and participate in photoshyntesis, photopro...

Projects

Projects (6)
Project
"Cytochrome P450 enzymes (CYPs) are involved in metabolic steps that provide structural diversity during the biosynthesis of carotenoids and their oxidative cleavage products called apocarotenoids." This project aims to discover the new CYP450 class enzymes that can metabolize apocarotenoids in plants.
Project
Modulation of carotenoid metabolism to enhance the contents of bioactive compounds involved in plant growth, development, and abiotic/biotic stress tolerance
Project
Finding the receptors of b-cc and dhA by using novel biochemical approaches in tobacco and Arabidopsis