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Jozef Van der Steen

Jozef Van der Steen
Alveus AB Consultancy

PhD

About

122
Publications
65,205
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2,054
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Introduction
beekeeping, honeybee, bumblebee rearing biomonitoring ecotoxicity environment pesticde

Publications

Publications (122)
Article
Full-text available
Pollen metabarcoding is emerging as a powerful tool for ecological research and offers unprecedented scale in citizen science projects for environmental monitoring via honey bees. Biases in metabarcoding can be introduced at any stage of sample processing and preservation is at the forefront of the pipeline. While in metabarcoding studies pollen ha...
Article
Full-text available
p>Due to their foraging behavior, honey bees interact with the landscape. As a result, honey bees and their brood will be exposed to pesticides through nectar and pollen entering the hive. Although these pathways seem rather straightforward, there are several steps between the entry of nectar and pollen and its consumption by the colony. One of the...
Article
Full-text available
Nectar of honeybee colonies has been used in order to identify heavy metals and establish the benefit of this type of studies as a tool for environmental management. For these goals, samples of nectar were obtained from Apis mellifera hives placed in the city of Córdoba (Spain) and its surroundings. Five stations (each with two hives) were selected...
Poster
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This poster was presented at the Apimondia 2019 in Montreal. Opinions and discussions are welcome, but the poster won't be changed anymore as it was already presented. To those of you who wanted the file of the chapter on the same topic: we're not allowed to share it, I'm sorry. By some reason, I can't send messages with the decline.
Article
Full-text available
To date, regulatory pesticide risk assessments have relied on the honey bee (Apis mellifera L.) (Hymenoptera: Apidae) as a surrogate test species for estimating the risk of pesticide exposure to all bee species. However, honey bees and non-Apis bees may differ in their susceptibility and exposure to pesticides. In 2017, a workshop ('Pesticide Expos...
Article
Een op maat gesneden varroabestrijding is, naast het zorgen voor een ruim en divers nectar- en stuifmeelaanbod, dé manier om wintersterfte terug te dringen. Of, voor de imker die ons driegangenmenu goed in de vingers heeft, zelfs geheel te voorkomen.1 Varroabestrijding (op maat gesneden voor de bijen) op stand is een aanpak die een stap verder gaat...
Article
Full-text available
In this study we show that honey bee colonies placed in a greenhouse for pollination of strawberry can simultaneously be used to indicate the presence of the plant pathogenic bacterium Erwinia pyrifoliae. This was demonstrated by using two methods of qualitative sacrificial and non-sacrificial bio sampling of the honey bee colony. A novel method fo...
Article
Full-text available
Abstract During the late spring of 2013 strawberry plants (Fragaria × ananassa Duch. ‘Elsanta’) were showing an intense blackening of their immature fruits, their fruit calyxes and the peduncule were found at several locations (greenhouses) in The Netherlands. Symptoms include brown petals, green young fruits turning brown, malformed fruits and bac...
Article
Full-text available
The 2013 EU ban of three neonicotinoids used in seed coating of pollinator attractive crops was put in place because of concern about declining wild pollinator populations and numbers of honeybee colonies. It was also concluded that there is an urgent need of good field data to fill knowledge gaps. In the meantime such data have been generated. Bas...
Article
Full-text available
Estimating the exposure of honeybees to pesticides on a landscape scale requires models of their spatial foraging behaviour. For this purpose, we developed a mechanistic, energetics-based model for a single day of nectar foraging in complex landscape mosaics. Net energetic efficiency determined resource patch choice. In one version of the model a s...
Article
Full-text available
In June 2008, a surveillance study for metals in honeybees was performed in the Netherlands. Randomly, 150 apiaries were selected. In each apiary, five colonies were sampled. Per apiary, the hive samples were pooled. The apiary sample was analysed for Al, As, Ba, Cd, Co, Cr, Cu, Li, Mn, Mo, Ni, Sb, Se, Sn, Sr, Ti, V and Zn. All metals could be dete...
Chapter
Full-text available
In this study we show that the honeybee colonies placed in a greenhouse for pollination of strawberry can simultaneously be used to indicate the presence of the plant pathogen Erwinia pyrifoliae. This was demonstrated using two methods of qualitative bio-indication: sacrificial and nonsacrificial subsampling. The non-sacrificial subsampler Beehold...
Article
Full-text available
p>In June 2008, a surveillance study for metals in honeybees was performed in the Netherlands. Randomly, 150 apiaries were selected. In each apiary, five colonies were sampled. Per apiary, the hive samples were pooled. The apiary sample was analysed for Al, As, Ba, Cd, Co, Cr, Cu, Li, Mn, Mo, Ni, Sb, Se, Sn, Sr, Ti, V and Zn. All metals could be de...
Article
Full-text available
Estimating the exposure of honeybees to pesticides on a landscape scale requires models of their spatial foraging behaviour. For this purpose, we developed a mechanistic, energetics-based model for a single day of nectar foraging in complex landscape mosaics. The hive population was assumed to select the resource patch with highest net energetic ef...
Article
Full-text available
Estimating the exposure of honeybees to pesticides on a landscape scale requires models of their spatial foraging behaviour. For this purpose, we developed a mechanistic, energetics-based model for a single day of nectar foraging in complex landscape mosaics. The hive population was assumed to select the resource patch with highest net energetic ef...
Article
Full-text available
Due to features that make them outstanding environmental bioindicator, colonies of Apis mellifera are being used to study environmental pollution. The primary objective of this research was to use honeybee colonies to identify heavy metals and determine their utility for environmental management. Five stations each with two A. mellifera hives were...
Conference Paper
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Since its establishment in 2008, the COLOSS (Prevention of honey bee COlony LOSSes) association has been successful in bringing together bee research scientists, bee health specialists and extension advisors to study the causes of global honey bee losses, and now has a substantial output of papers published in refereed scientific journals. The newl...
Article
Full-text available
Global declines of bumble bees and other pollinator populations are of concern because of their critical role for crop production and maintenance of wild plant biodiversity. Although the consensus among scientists is that the interaction of many factors, including habitat loss, forage scarcity, diseases, parasites, and pesticides potentially plays...
Article
Full-text available
The number of bees, amount of brood and haemolymph vitellogenin titre are parameters to establish the vitality of a honey bee colony. Increasing numbers of bees during summer until autumn; increasing amounts of brood in spring towards summer followed by a decrease; and low haemolymph vitellogenin titres in foraging bees and high vitellogenin titres...
Article
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“C.S.I. Pollen” aims to be the largest investigation on pollen diversity available to honey bee colonies in Europe. This should be accomplished by an international approach involving beekeepers to participate as citizen scientists.
Article
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The aim of our study was to explore whether honeybees (Apis mellifera L.) could be used as a reliable alternative to the standard mechanical devices for monitoring of air quality, in particular with respect to the concentration of the heavy metals cadmium (Cd), lead (Pb) and vanadium (V). We therefore tested whether the concentrations of these meta...
Article
Full-text available
Although honeybee risk assessment for chemicals has been rigorously revised recently, methods and techniques available for non-apis pollinators are scarce. An ICPPR working group " non-apis " was established in 2013 to address these knowledge gaps. Acute contact tests were designed and performed with solitary bees Osmia sp. but still require furthe...
Article
Full-text available
Inwinteren is het slotstuk van het bijenjaar, een soort “Grande Finale” na een seizoen hard werken met de bijen. We geven onze bijen voldoende voedsel om de winter goed door te komen en in het voorjaar weer fris en vrolijk aan de slag te kunnen gaan. Gelukkig is het eenvoudig mits er aan enkele voorwaarden voldaan wordt. Waarom winteren we onze bij...
Article
Om een inschatting te kunnen maken van het risico dat honingbijen blootgesteld worden aan gewasbeschermingsmiddelen, andere stoffen zoals atmosferische depositie van fijnstof en organismen zoals plantpathogene microorganismen, is in opdracht van het Ministerie van EZ/Landbouw een samenvatting gemaakt van de informatie, beschikbaar over de aantrekke...
Article
Full-text available
Bijen en hommels zijn door hun bouw en gedrag goede bestuivers. Maar ze kunnen meer. Ze zijn ook heel geschikt om antagonistische micro-organismen voor ziektebestrijding over te brengen én informatie vanuit het veld over ziekten mee terug te nemen. Belangrijk voor al deze taken is: hoe houden we ze actief onder de ‘nieuwe’ teeltomstandigheden in de...
Article
Full-text available
In 1990 is in Nederland een werkgroep opgericht om jaarlijks gevallen van massale bijensterfte te inventariseren die volgens getroffen imkers veroorzaakt zijn door blootstelling aan gewasbeschermingsmiddelen (Oomen, 1992). De werkgroep jubileert dit jaar en heeft daarom haar bevindingen met spuitschade in de afgelopen 25 jaar samengevat
Article
Full-text available
In 1990 is in Nederland een werkgroep opgericht om jaarlijks gevallen van massale bijensterfte te inventariseren die volgens getroffen imkers veroorzaakt zijn door blootstelling aan gewasbeschermingsmiddelen (Oomen, 1992). De werkgroep jubileert dit jaar en heeft daarom haar bevindingen met spuitschade in de afgelopen 25 jaar samengevat
Article
Full-text available
Dit artikel is een compilatie van het Wageningen-UR PRI rapport 606 ’Factoren die het foerageergedrag van honingbijen bepalen (deel I)’. In dit rapport wordt het haalgedrag van de honingbij beschreven: hoe wordt het bepaald en wat wordt verzameld en hoe. Daarnaast is in het rapport een drachtplantenlijst opgenomen (deel II). Hoe bijen drachten bezo...
Article
Full-text available
n 2011, two polytunnel greenhouse experiments were conducted on seed production farms, one under conventional conditions in the South-West (Rilland) and the other under organic conditions in the East (Voorst) of the Netherlands, to study transmission routes of Xanthomonas campestris pv. campestris (Xcc), that can result in seed infections of caulif...
Article
Is bijensterfte het werk van neonicotinoïden? Ja, zeggen en denken velen. Volgens hen zijn de neo’s rechtstreeks verantwoordelijk voor de hoge sterfte onder bijen. Nee, toont Wagenings onderzoek aan. Intussen lijkt het met de sterfte voorzichtig de goede kant op te gaan.
Article
De wereldwijde afname van bijen en ander bestuivers heeft grote consequenties voor de voedselzekerheid. Verdere verslechtering van de leefomstandigheden kan leiden tot het plotseling uitsterven van bestuivende insectensoorten. In Europa dreigt inmiddels een tekort aan honingbijen voor de bestuiving van landbouwgewassen. De vraag naar bestuivers gro...
Article
Full-text available
A variety of methods are used in honey bee research and differ depending on the level at which the research is conducted. On an individual level, the handling of individual honey bees, including the queen, larvae and pupae are required. There are different methods for the immobilising, killing and storing as well as determining individual weight of...
Article
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Summary In this article we provide guidelines on statistical design and analysis of data for all kinds of honey bee research. Guidelines and selection of different methods presented are, at least partly, based on experience. This article can be used: to identify the most suitable analysis for the type of data collected; to optimise one’s experiment...
Article
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Modern agriculture often involves the use of pesticides to protect crops. These substances are harmful to target organisms (pests and pathogens). Nevertheless, they can also damage non-target animals, such as pollinators and entomophagous arthropods. It is obvious that the undesirable side effects of pesticides on the environment should be reduced...
Article
Full-text available
Honey bee virus research is an enormously broad area, ranging from subcellular molecular biology through physiology and behaviour, to individual and colony-level symptoms, transmission and epidemiology. The research methods used in virology are therefore equally diverse. This article covers those methods that are very particular to virological rese...
Article
Full-text available
This paper covers measures of field colony strength, by which we mean population measures of adult bees and brood. There are generally two contexts in which an investigator wishes to measure colony strength: 1. at the beginning of a study as part of manipulations to produce uniform colonies and reduce experimental error and; 2. as response variable...
Article
Full-text available
Globally, agricultural production systems are under pressure to meet multiple challenges: to sustain or increase production from the same area of land and reduce negative impacts on the environment amid uncertainties resulting from climate change. As farming systems adapt to meet these challenges, one of agriculture’s greatest assets in meeting the...
Article
Full-text available
In order to conduct a proper risk assessment of pesticides to bees, information is needed in three areas: (i) the toxicity of the pesticide; (ii) the probability of bee exposure to that pesticide; and (iii) the population dynamics of the bee species in question. Information was collected on such factors affecting pesticide risk to (primarily wild)...
Article
Om een beter inzicht te krijgen in de praktijk van de Nederlandse imker en de bijenziekten, is in het kader van het BIJ-1 project, het Nederlands Bijenvolk Onderzoek (NBO) opgezet. Het onderzoek uitgevoerd door Bijen@wur (onderdeel van Plant Research International) en in samenwerking met het Nederlandse Centrum voor Bijenonderzoek (NCB). Het onderz...
Article
Full-text available
De varroa mijtziekte (Varroa destructor) is de belangrijkste bedreiging van de Europese honingbij. Wintersterfte van honingbijen is in de meeste gevallen toe te schrijven aan deze ziekte. Deze tweede, licht gewijzigde druk biedt informatie over de biologie van varroa, de effecten van varroa op honingbijen en de bestrijding van varroa.
Article
Deze demonstratieproef is opgezet als een Citizen Scientist (CS) project waarbij het praktische veldwerk en de waarnemingen, op aanwijzing van en gecoördineerd door PRI bijen@wur, uitgevoerd werden door bijenhouders. De analyses, interpretatie van de data en de rapportage zijn uitgevoerd door PRI bijen@wur. Voor de demonstratieproef 2012-2013 is aa...
Article
Full-text available
Adult honey bees are maintained in vitro in laboratory cages for a variety of purposes. For example, researchers may wish to perform experiments on honey bees caged individually or in groups to study aspects of parasitology, toxicology, or physiology under highly controlled conditions, or they may cage whole frames to obtain freshly emerged workers...
Article
Full-text available
Pollinators are important components of biodiversity and provide a key ecosystem service through pollination (Klein et al., 2007). Honeybees, mainly Apis mellifera, are the most economically valuable pollinators for crop monocultures worldwide (Wantanabe, 1994), however, for several high-value crops, e.g., coffee, Apis pollination is less effective...
Article
Full-text available
Recent elevated winter loss of honey bee colonies is a major concern. The presence of the mite Varroa destructor in colonies places an important pressure on bee health. V. destructor shortens the lifespan of individual bees, while long lifespan during winter is a primary requirement to survive until the next spring. We investigated in two subsequen...
Data
Cumulative survival curves for 2005/2006 (top) and 2006/2007 (bottom), per acaricide treatment (left), and for the marking date of the cohorts (right). Cumulative survival curves were calculated from the Cox Proportional Hazards Models for cohorts of bees marked. For the survival analysis, we had 6398 uncensored cases and 346 censored cases for 200...
Data
Mean survivorship curves for cohorts of bees marked in 2005/2006 (left) and 2006/2007 (right). Cohorts of bees were marked at 14-day intervals for each acaricide treatment: July (open diamonds), August (grey squares), September (dark grey triangles), and not treated at all (black circles). Each line shows the mean survival of 1 to 4 cohorts. During...
Article
Full-text available
In honey bee studies focusing on physiology, disease diagnosis or bio indication, bees are sampled from the colony. This raises the question of where in the colony samples must be taken from for specific study objectives. In this study we recorded where bees of known age are found in the hive. We recorded in a single brood box with ten frames in Au...
Article
Full-text available
In order to conduct a proper risk assessment of pesticides to bees, information is needed in three areas: (i) the toxicity of the pesticide; (ii) the probability of bee exposure to that pesticide; and (iii) the population dynamics of the bee species in question. Information was collected on such factors affecting pesticide risk to (primarily wild)...
Article
Tijdens het symposium van bijen@wur PRI op 18 maart vertelde Sjef van der Steen over zijn onderzoek in 2009 en 2010 naar invloed van variatie en continuïteit in de aanvoer van stuifmeel op de vitaliteit van bijenvolken. Voor Bijenhouden schreef hij een artikel over zijn aanpak en de uitkomsten, met tot slot advies voor de imker.
Article
Full-text available
Honeybees (Apis mellifera L.) have great potential for detecting and monitoring environmental pollution, given their wide-ranging foraging behaviour. Previous studies have demonstrated that concentrations of metals in adult honeybees were significantly higher at polluted than at control locations. These studies focused at a limited range of heavy m...
Article
Veel bloemen hebben insecten, zoals bijvoorbeeld bijen, nodig om stuifmeel van de ene naar de ander bloem te brengen. Maar dat zal de bijen strikt genomen worst wezen. De bijen bezoeken de bloemen niet om het stuifmeel van de ene bloem naar de andere te transporteren maar om voedsel te verzamelen. Er is sprake van wederkerige afhankelijkheid. Alles...
Article
Dat de varraomijt bestreden moet worden is voor iedere imker duidelijk. Niet bestrijden leidt tot zwakke volken en wintersterfte. Onderzoeken van PRI Bijen@wur en vele buitenlandse onderzoeksinstellingen komen, als het gaat over wintersterfte, altijd hetzelfde uit: de varroamijt is oorzaak nummer één
Article
Full-text available
Varroa is de belangrijkste ziekte van de Europese honingbij. Daar zijn onderzoekers in Nederland, maar ook in de rest van de wereld het over eens (Neumann & Carreck, 2010). Zelfs na jaren van praktijkervaring en experimenteel onderzoek blijft deze parasiet onze bijen teisteren. Dat is niet zo gek, want in de 27 jaar dat varroa in Nederland wordt aa...
Article
De varroa mijtziekte (Varroa destructor) is de belangrijkste bedreiging van de Europese honingbij. Wintersterfte van honingbijen is in de meeste gevallen toe te schrijven aan deze ziekte. Deze brochure van de WUR biedt informatie over de biologie van varroa, de effecten van varroa op honingbijen en de bestrijding van varroa.