Jothi Dinesh Kumar

Jothi Dinesh Kumar
University of St Andrews · School of Physics and Astronomy

PhD

About

35
Publications
5,565
Reads
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827
Citations
Additional affiliations
June 2014 - October 2015
University of Liverpool
Position
  • PostDoc Position

Publications

Publications (35)
Article
Three dimensional (3D) bioprinting of multiple cell types within optimised extracellular matrices has the potential to more closely model the 3D environment of human physiology and disease than current alternatives. In this study, we used a multi-nozzle extrusion bioprinter to establish models of glioblastoma made up of cancer and stromal cells pri...
Article
Full-text available
Fluorescence imaging is an indispensable tool in biology, with applications ranging from single‐cell to whole‐animal studies and with live mapping of neuronal activity currently receiving particular attention. To enable fluorescence imaging at cellular scale in freely moving animals, miniaturized microscopes and lensless imagers are developed that...
Article
Full-text available
The chemokine-like peptide, chemerin, stimulates chemotaxis in several cell types. In this study we examined the expression of putative chemerin receptors in gastric cancer and the action of chemerin on cancer cell migration and invasion. Immunohistochemical studies of gastric tumors identified expression of two putative receptors, chemokine-like r...
Conference Paper
We report the application of semiconductor nanodisk lasers within living cells. Our lasers have volumes 1000-fold smaller than eukaryotic nuclei, ultralow pulse energy lasing thresholds (Eth≈0.13pJ), and provide excellent spectral stability.
Article
Full-text available
Molecular dyes, plasmonic nanoparticles and colloidal quantum dots are widely used in biomedical optics. Their operation is usually governed by spontaneous processes, which results in broad spectral features and limited signal-to-noise ratio, thus restricting opportunities for spectral multiplexing and sensing. Lasers provide the ultimate spectral...
Article
Full-text available
This work reports the use of an infrared spectroscopic version of scanning near-field optical microscopy (IR-SNOM) and shows that it is possible to reveal subcellular entities via their chemical constituents in a label-free human oesophageal adenocarcinoma cell (OE33). This work presents the first high spatial resolution (∼0.15 μm) study of feature...
Article
Full-text available
Analysis of secretomes critically underpins the capacity to understand the mechanisms determining interactions between cells and between cells and their environment. In the context of cancer cell micro-environments, the relevant interactions are recognised to be an important determinant of tumor progression. Global proteomic analyses of secretomes...
Preprint
Nanophotonic objects like plasmonic nanoparticles and colloidal quantum dots can complement the functionality of molecular dyes in biomedical optics. However, their operation is usually governed by spontaneous processes, which results in broad spectral features and limited signal-to-noise ratio, thus restricting opportunities for spectral multiplex...
Article
Full-text available
Matrix metalloproteinase (MMP)-7, unlike many MMPs, is typically expressed in epithelial cells. It has been linked to epithelial responses to infection, injury, and tissue remodeling including the progression of a number of cancers. We have now examined how MMP-7 expression changes in the progression to esophageal adenocarcinoma (EAC), and have stu...
Article
Full-text available
In-vitro brain tumour models are valuable tools both to study cancer biology and for successful pre-clinical testing of anticancer drugs. However, substantial challenges remain before we can create pathologically-relevant models which closely mimic the microenvironment of human brain tumours, their growth characteristics and their response to thera...
Conference Paper
Here, we demonstrate the successful application of organic light-emitting diodes in optogenetics to control neurons in fruit fly larvae and explore their further use as a light source in fluorescence microscopy.
Article
Full-text available
A single human oesophageal adenocarcinoma cell (OE33) has been imaged using aperture infrared scanning near-field optical microscopy (IR-SNOM) in transmission and reflection and also by Fourier-transform infrared (FTIR) microspectroscopy in transmission only. This work presents the first images obtained in both transmission and reflection of the sa...
Article
Full-text available
Regenerative medicine therapies (RMTs) hold enormous potential for a variety of currently incurable conditions with high unmet clinical need. Most progress in this field to date has been achieved with cell-based RMTs, with over a thousand clinical trials performed up to 2015. However, lack of adequate safety and efficacy data is currently limiting...
Article
Full-text available
The well-known action of the gastric hormone gastrin in stimulating gastric acid secretion is mediated by activation of cholecystokinin-2 receptors (CCK2R). The latter are expressed by a variety of cell types suggesting that gastrin is implicated in multiple functions. During wound healing in the stomach CCK2R may be expressed by myofibroblasts. We...
Article
Glycogen Synthase Kinase-3 (GSK3 or GSK-3) is a promiscuous protein kinase and its phosphorylation of its diverse substrates has major influences on many areas of physiology and pathology, including cellular metabolism, lineage commitment and neuroscience. GSK3 was one of the first identified substrates of the heavily studied oncogenic kinase AKT,...
Article
Glucagon-like peptide (GLP)-2 stimulates intestinal epithelial proliferation by acting, in part, via IGF release from sub-epithelial myofibroblasts. The response of myofibroblasts to GLP-2 remains incompletely understood. We studied the action of GLP-2 on myofibroblasts from colon cancer and adjacent tissue, and the effects of conditioned medium fr...
Article
Despite massive investments in drug research and development, the significant decline in the number of new drugs approved or translated to clinical use raises the question, whether single targeted drug discovery is the right approach. To combat complex systemic diseases that harbour robust biological networks such as cancer, single target intervent...
Article
Connective tissue growth factor (CTGF) has been reported in gastric adenocarcinoma and in carcinoid tumors. The aim of this study was to explore a possible link between CTGF and gastrin in gastric epithelial cells and to study the role of CTGF in gastrin induced migration and invasion of AGS-GR cells. The effects of gastrin were studied using RT-qP...
Article
Full-text available
Background: Stromal cells, including cancer-associated myofibroblasts (CAMs), are recognised to be determinants of cancer progression, but the mechanisms remain uncertain. The chemokine-like protein, chemerin, is upregulated in oesophageal squamous cancer (OSC) CAMs compared with adjacent tissue myofibroblasts (ATMs). In this study, we hypothesise...
Article
Full-text available
Stromal cells influence epithelial function both in health and disease. Myofibroblasts are abundant stromal cells that influence the cellular microenvironment by release of extracellular matrix (ECM) proteins, growth factors, proteases, cytokines and chemokines. Cancer-associated myofibroblasts (CAMs) differ from adjacent tissue (ATMs), and normal...
Article
Full-text available
Mesenchymal stem cells (MSCs) play important roles in tissue repair and cancer progression. Our recent work suggests that some mesenchymal cells, notably myofibroblasts exhibit regulated exocytosis resembling that seen in neuroendocrine cells. We now report that MSCs also exhibit regulated exocytosis. Both a G-protein coupled receptor agonist, chem...
Article
Full-text available
The pyloric antral hormone gastrin plays a role in remodelling of the gastric epithelium but the specific targets of gastrin that mediate these effects are poorly understood. Glandular epithelial cells of the gastric corpus express matrix metalloproteinase (MMP)-1 which is a potential determinant of tissue remodelling; some of these cells express t...
Article
Full-text available
Stromal cells such as myofibroblasts influence tumor progression. The mechanisms are unclear but may involve effects on both tumor cells and recruitment of bone marrow-derived mesenchymal stromal cells (MSCs) which then colonize tumors. Using iTRAQ and LC-MS/MS we identified the adipokine, chemerin, as overexpressed in esophageal squamous cancer as...
Article
Gastric cancer is the second commonest cause of cancer-related death worldwide. Within these tumors, it is increasingly recognised that signaling between myofibroblasts, an important stromal cell type, and cancer cells results in proliferation, invasion and metastasis. However, little is known of the range of proteins secreted by stromal cells ie t...
Article
Cancer progression involves changes in extracellular proteolysis but the contribution of stromal cell secretomes to the cancer degradome remains uncertain. We have now defined the secretome of a specific stromal cell type, the myofibroblast, in gastric cancer, and its modification by proteolysis. SILAC labelling and COFRADIC isolation of methionine...
Article
Full-text available
Tumor progression has been linked to changes in the stromal environment. Myofibroblasts are stromal cells that are often increased in tumors but their contribution to cancer progression is not well understood. Here, we show that the secretomes of myofibroblasts derived from gastric cancers [cancer-associated myofibroblasts (CAMs)] differ in a funct...
Article
Full-text available
Infection with Staphylococcus aureus was initially considered a major problem in hospitals, but over the last few decades the incidence of community-acquired infection has also increased. Paper currency has recently been identified as another mode of spread by which community-acquired S. aureus infection may be transmitted, since paper currency is...

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Projects

Projects (3)
Archived project
To understand how Helicobacter pylori affect the development of gastric cancer
Archived project
To bioprint physiologically relevant 3D cancer tissue model that has the potential to be a powerful tool for future drug discoveries and cancer biology studies.