Josefina Campos

Josefina Campos
Administración Nacional de Laboratorios e Institutos de Salud (Argentina) | ANLIS · Departamento Bacteriologia

About

65
Publications
10,416
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1,327
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Introduction
With a degree in Biochemistry, he began working at the INEI-ANLIS Malbrán in Argentina in foodborne pathogen surveillance. She is currently the Director of the National Genomics and Bioinformatics Center at ANLIS Malbrán, working at the national level integrating genomics in the surveillance system for infectious and non-transmissible diseases. She is the responsible of the newly created National Genomics Network in Argentina.

Publications

Publications (65)
Article
Hantavirus Pulmonary Syndrome (HPS) is an emerging infectious disease caused by orthohantaviruses in the Americas. In Argentina, since 1995, several reservoirs and virus variants have been described, but the northeastern and central endemic zones in the country include an area without human or rodent infections, despite sharing rodent species with...
Article
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Objective: We aimed to describe a colistin (COL)-resistant (R) Chromobacterium violaceum (Cvi) isolate from a septic patient in Argentina expressing a previously unknown gene, blaCVI-1. Methods: In 2019, a 12 year old child was injured with a thorn in a lagoon. The child was hospitalized due to sepsis and multiple abscesses. Cvi was isolated fro...
Article
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Azithromycin combined with ceftriaxone is the recommended dual therapy for uncomplicated gonorrhea in many countries. Nevertheless, the increasing prevalence of azithromycin resistance compromises the effectiveness of this treatment strategy. From 2018 to 2022, we collected 13 gonococcal isolates with high-level azithromycin resistance (MIC ≥ 256 μ...
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Staphylococcus aureus remains one of the leading causes of infections worldwide and a common cause of bacteraemia. However, studies documenting the epidemiology of S. aureus in South America using genomics are scarce. We hereby report on the largest genomic epidemiology study to date of both methicillin-resistant S. aureus (MRSA) and methicillin-su...
Article
Objectives: The worldwide dissemination of carbapenemase-producing Escherichia coli lineages belonging to high-risk clones poses a challenging public health menace. The aim of this work was to investigate genomic features of a colonizing multidrug resistant strain of KPC-producing E. coli from our institution. Methods: WGS was done by Illumina M...
Preprint
Full-text available
Staphylococcus aureus remains one of the leading causes of infections worldwide and a common cause of bacteremia. However, studies documenting the epidemiology of S. aureus in South America (SA) using genomics are scarce. We hereby report on the largest to date genomic epidemiology study of both methicillin-resistant S. aureus (MRSA) and methicilli...
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Objectives The emergence of blaKPC-2 within nosocomial settings has become a major public health crisis worldwide. Our aim was to perform WGS of three KPC-producing Gram-Negative Bacilli (KPC-GNB) strains isolated from a hospitalized patient to identify acquired antimicrobial resistance genes (ARGs). Methods WGS was made using Illumina MiSeq-I, an...
Article
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According to the World Health Organization, carbapenem-resistant Enterobacteriaceae (CRE) belong to the highest priority group for the development of new antibiotics. Argentina-WHONET data showed that Gram-negative resistance frequencies to imipenem have been increasing since 2010 mostly in two CRE bacteria: Klebsiella pneumoniae and Enterobacter c...
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Bats are reservoirs of diverse coronaviruses (CoVs), including progenitors of severe acute respiratory syndrome CoV (SARS-CoV) and SARS-CoV-2. In the Americas, there is a contrast between alphacoronaviruses (alphaCoVs) and betaCoVs: while cospeciation prevails in the latter, alphaCoV evolution is dominated by deep and recent host switches. AlphaCoV...
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Objectives : Enterobacter cloacae complex (ECC) has lately awakened interest due to its increasing resistance to carbapenems codified by several genes all over the globe. Even though there are some sequence types (ST) which represent high-risk clones, there is substantial clonal diversity in the ECC. This work aimed to perform whole-genome sequenci...
Article
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Extraintestinal pathogenic Escherichia coli (ExPEC) causes infections outside the intestine. Particular ExPEC clones, such as clonal complex (CC)/sequence type (ST)131, have been known to sequentially accumulate antimicrobial resistance that starts with chromosomal mutations against fluoroquinolones, followed with the acquisition of bla CTX−M−15 an...
Article
The spread of carbapenem-resistant Enterobacterales has raised concern in clinical settings due to the limited therapeutic options available. OXA-48-like enzymes are still sporadic in South America. The aim of this study was to characterize a multidrug-resistant Escherichia coli isolate from a hospitalized patient in Buenos Aires city. The isolate...
Article
Purpose Rapid spread of multidrug resistant Gram-negative bacilli (MDR-GNB) infection in Coronavirus disease (COVID-19) critically ill patients was observed even in those without underlying diseases and in all age groups. We conducted a prospective cohort study to assess the risk factors for acquisition of MDR-GNB infection in COVID-19 patients and...
Article
Purpose The rational use of antimicrobials is essential and can be achieved through antimicrobial stewardship programs. Although several studies identify the clonal relationships of nosocomial carbapenemase KPC-producing Klebsiella pneumoniae (KPC-Kp) strains, none of them, at our knowledge, investigates at the same time clonal status of colonizing...
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Background: The Public Health Alliance for Genomic Epidemiology (PHA4GE) (https://pha4ge.org) is a global coalition that is actively working to establish consensus standards, document and share best practices, improve the availability of critical bioinformatics tools and resources, and advocate for greater openness, interoperability, accessibility...
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Purpose This article describes an outbreak of E. anophelis in a neonatal unit in a private hospital in Buenos Aires, Argentina, in which the source of infection was identified, therefore allowing the prompt intervention of the infection control team. The awareness of E. anophelis infections and the collaboration between infection control teams and...
Article
Objectives Isolation of colistin- and carbapenem-resistant Klebsiella pneumoniae (CCR-Kp) is increasing in hospital settings worldwide, which is related to increased morbidity, mortality and healthcare costs. The aim of this work was to perform whole-genome sequencing (WGS), genomic and phylogenetic analysis, and conjugation assays of an extensivel...
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Little is known about the genetic diversity of Salmonella enterica serovar Typhi ( S . Typhi) circulating in Latin America. It has been observed that typhoid fever is still endemic in this part of the world; however, a lack of standardized blood culture surveillance across Latin American makes estimating the true disease burden problematic. The Col...
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Azithromycin-nonsusceptible Neisseria gonorrhoeae strains are an emerging global public health threat. During 2015-2018, the prevalence of azithromycin-nonsusceptible gonococcal infection increased significantly in Argentina. To investigate the genomic epidemiology and resistance mechanisms of these strains, we sequenced 96 nonsusceptible isolates...
Article
The aim of this study was to set up a simple protocol to concentrate SARS-CoV-2 from sewage, which can be implemented in laboratories with minimal equipment resources. The method avoids the need for extensive purification steps and reduces the concentration of potential inhibitors of RT-qPCR contained in sewage. The concentration method consists of...
Article
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Specific lineages of serogroup O1 Vibrio cholerae are notorious for causing cholera pandemics, of which there have been seven since the 1800s. Much is known about the sixth pandemic (1899–1923) and the ongoing seventh pandemic (1961–present), but we know very little about the bacteriology of pandemics 1 to 5. Moreover, although we are learning abou...
Preprint
The aim of this study was to set up a simple protocol to concentrate SARS-CoV-2 from sewage, which can be implemented in laboratories with minimal equipment resources. The method avoids the need for extensive purification steps and reduces the concentration of potential inhibitors of RT-qPCR contained in sewage. The concentration method consists of...
Article
Full-text available
Background Whole-genome sequencing has shown that the Mycobacterium tuberculosis infection process can be more heterogeneous than previously thought. Compartmentalized infections, exogenous reinfections, and microevolution are manifestations of this clonal complexity. The analysis of the mechanisms causing the microevolution —the genetic variabilit...
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Background Whole genome sequencing (WGS) is increasingly used for pathogen identification and surveillance. Aim We evaluated costs and benefits of routine WGS through case studies at eight reference laboratories in Europe and the Americas which conduct pathogen surveillance for avian influenza (two laboratories), human influenza (one laboratory) a...
Preprint
Full-text available
Background Whole-genome sequencing has shown that the Mycobacterium tuberculosis infection process can be more heterogeneous than previously thought. Compartmentalized infections, exogenous reinfections, and microevolution are manifestations of this clonal complexity. The analysis of the mechanisms causing the microevolution —the genetic variabilit...
Article
BACKGROUND From November 2018 through February 2019, person-to-person transmission of Andes virus (ANDV) hantavirus pulmonary syndrome occurred in Chubut Province, Argentina, and resulted in 34 confirmed infections and 11 deaths. Understanding the genomic, epidemiologic, and clinical characteristics of person-to-person transmission of ANDV is cruci...
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Objective: This study aimed to determine the prevalence of fecal carriage of antibiotic-resistant Escherichia coli of healthy household dogs with an emphasis on extended-spectrum β-lactamases (ESBL), AmpC-type β-lactamases and resistance to quinolones. Materials and Methods : Rectal swabs were collected from 74 dogs without any clinical evidence o...
Article
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In order to control and eradicate epidemic cholera, we need to understand how epidemics begin, how they spread, and how they decline and eventually end. This requires extensive sampling of epidemic disease over time, alongside the background of endemic disease that may exist concurrently with the epidemic. The unique circumstances surrounding the A...
Preprint
Full-text available
The Public Health Alliance for Genomic Epidemiology (PHA4GE) (https://pha4ge.org) is a global coalition that is actively working to establish consensus standards, document and share best practices, improve the availability of critical bioinformatic tools and resources, and advocate for greater openness, interoperability, accessibility and reproduci...
Article
Full-text available
Antibiotic resistance is on the rise, leading to an increase in morbidity and mortality due to infectious diseases. Klebsiella pneumoniae is a Gram-negative bacterium that causes bronchopneumonia, abscesses, urinary tract infection, osteomyelitis, and a wide variety of infections. The ubiquity of this microorganism confounds with the great increase...
Article
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Salmonella Typhi (S. Typhi) is the causative agent of typhoid fever; a systemic disease affecting ~20 million people per year globally. There are little data regarding the contemporary epidemiology of typhoid in Latin America. Consequently, we aimed to describe some recent epidemiological aspects of typhoid in Colombia using cases reported to the N...
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PulseNet Latin America and Caribbean (PNLAC) was established in 2003 and is one of seven Regional networks within PulseNet International. The main objectives of the network are to strengthen national and regional laboratory-based foodborne disease surveillance for early detection and investigation of outbreaks to setup control and prevention strate...
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Objectives: Since blaKPC-2-producing Klebsiella pneumoniae (KPC-Kp) are related to the successful dissemination of a major clone defined as sequence type 258 (ST258), our goal was to perform whole-genome sequencing (WGS) of the first colistin-resistant K. pneumoniae strain (Kpn666) carrying blaKPC-2 identified in Uruguay in 2011 in order to identi...
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Lactobacillus helveticus is a lactic acid bacterium used traditionally in the dairy industry, especially in the manufacture of cheeses. We present here the 2,141,841-bp draft genome sequence ofL. helveticusstrain ATCC 12046, a potential starter strain for improving cheese production.
Article
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Wave upon wave of disease The cholera pathogen, Vibrio cholerae , is considered to be ubiquitous in water systems, making the design of eradication measures apparently fruitless. Nevertheless, local and global Vibrio populations remain distinct. Now, Weill et al. and Domman et al. show that a surprising diversity between continents has been establi...
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PulseNet International is a global network dedicated to laboratory-based surveillance for food-borne diseases. The network comprises the national and regional laboratory networks of Africa, Asia Pacific, Canada, Europe, Latin America and the Caribbean, the Middle East, and the United States. The PulseNet International vision is the standardised use...
Poster
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Salmonella enterica serovar Typhi (S. Typhi), is the pathogen responsible of Typhoid fever; a systemic disease that affect 20.6 millions of persons around the world each year, causing an estimated of 223.000 deaths. Ingestion of food or water contaminated by acutely infected persons or chronic typhoid carriers is the most common form of transmissio...
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Objective: Shigella sonnei is a globally-important diarrhoeal pathogen tracked through the surveillance network PulseNet Latin America and Caribbean (PNLA&C), which participates in PulseNet International. PNLA&C laboratories use common molecular techniques to track pathogens causing foodborne illness. We aimed to demonstrate the possibility and ad...
Article
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The population of Salmonella enterica serovar Typhi (S. Typhi), the causative agent of typhoid fever, exhibits limited DNA sequence variation, which complicates efforts to rationally discriminate individual isolates. Here we utilize data from whole-genome sequences (WGS) of nearly 2,000 isolates sourced from over 60 countries to generate a robust g...
Conference Paper
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La metodología de secuenciación de genoma completo (SGC) y el análisis de secuencias de genomas bacterianos se aplica desde sus inicios en diferentes áreas de investigación. En la actualidad, está siendo utilizada en salud pública, en la identificación de especies, genes de virulencia y resistencia, y en investigación de brotes. Esta aplicación es...
Article
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An epidemiological paradox surrounds Salmonella enterica serovar Enteritidis. In high-income settings, it has been responsible for an epidemic of poultry-associated, self-limiting enterocolitis, whereas in sub-Saharan Africa it is a major cause of invasive nontyphoidal Salmonella disease, associated with high case fatality. By whole-genome sequence...
Article
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Shigella sonnei is an emergent cause of diarrheal disease in middle-income countries. The organism causes endemic disease and is also associated with sporadic outbreaks in susceptible populations. In 2010 and 2011 there were two suspected outbreaks of diarrheal disease caused by S. sonnei in La Pampa province in central Argentina. Aiming to confirm...
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The emergence of multidrug-resistant (MDR) typhoid is a major global health threat affecting many countries where the disease is endemic. Here whole-genome sequence analysis of 1,832 Salmonella enterica serovar Typhi (S. Typhi) identifies a single dominant MDR lineage, H58, that has emerged and spread throughout Asia and Africa over the last 30 yea...
Article
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Adult chinchillas (Chinchilla lanigera) that had suddenly died in a commercial farm located in La Plata City, Buenos Aires Province, Argentina, in July 2012 were macroscopically, histopathologically, and microbiologically examined. Salmonella enterica serovar Typhimurium (S. Typhimurium) was isolated from the liver, spleen, heart, lungs, kidneys an...
Article
Salmonella serovar Dublin causes disease in cattle and leads to considerable production losses. In humans, severe invasive disease and high mortality rates are reported. The presently available typing methods provide insufficient discrimination within Salm. Dublin for epidemiological investigations. In this study, we developed a multiple-locus vari...
Poster
Full-text available
Introducción Salmonella Abortusequi es una de las serovariedades adaptadas a una determinada especie animal, la equina, en la cual produce abortos, retención placentaria, orquitis y septicemia en neonatos. A nivel mundial, hay descriptos brotes en África y Asia, pero existen muy pocos reportes en Europa y EEUU; y en nuestro país no se registran cas...
Conference Paper
Full-text available
La salmonelosis es una enfermedad infectocontagiosa y zoonótica producida por bacterias del género Salmonella que afecta al hombre y a los animales. Algunas serovariedades están adaptadas a determinadas especies animales, tal es caso de Salmonella Abortusequi cuyo huésped son los equinos, y puede ser agente primario de abortos o secundario a la inf...
Conference Paper
Full-text available
El objetivo del presente estudio es describir un brote de abortos en yeguas por Salmonella enterica serovarovariadad Abortusequi.En nuestro país no se registra información de organismos oficiales desde hace más de 40 años, por lo cual deberá considerarse como una enfermedad emergente.
Article
PulseNet Latin America and Caribbean Network (PulseNet LA and C) works together with PulseNet International sharing molecular epidemiologic information for the recognition and investigation of foodborne disease outbreaks.The participants of PulseNet LA and C perform standardized pulsed field gel electrophoresis (PFGE) protocols and analysis generat...
Article
Las enfermedades transmitidas por alimentos (ETA) y otras enfermedades entéricas infecciosas ocurren a menudo como brotes y son causa de morbilidad y mortalidad en todo el mundo. En el Perú, son un importante problema de salud pública y son causados por una gran variedad de agentes infecciosos. Para la investigación epidemiológica se utiliza una va...
Article
Full-text available
Foodborne diseases and other enteric infections often occur as outbreaks and cause morbidity and mortality all over the world. In Perú, they represent a serious public health problem, and are caused by a great variety of infectious agents. For epidemiological research, a wide array of typification methods are used. One of the most important tools f...
Article
Full-text available
Foodborne diseases and other enteric infections often occur as outbreaks and cause morbidity and mortality all over the world. In Perú, they represent a serious public health problem, and are caused by a great variety of infectious agents. For epidemiological research, a wide array of typification methods are used. One of the most important tools f...

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