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  • José A Teixeira
José A Teixeira

José A Teixeira
University of Minho · Institute for Biotechnology and Bioengineering - Centre of Biological Engineering

About

963
Publications
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Publications

Publications (963)
Article
Full-text available
Recently, there are two main movements gaining public attention: a “green revolution” focused on the use of sustainable processes and the increasing concerns with health-promoting diets and the recognition of the gut microbiota and its effects on the treatment and prevention of several diseases. Microalgae and macroalgae arise as an interesting alt...
Article
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Short chain fatty acids (SCFA) are hypothesised to play a key role in the microbiota-gut-brain crosstalk. However, studies that evaluate the effect of microbiota-targeted interventions, such as prebiotics, probiotics or diet are still sparse, particularly in humans. In this work, the products of fermentation of prebiotic-enriched media by bacteria...
Article
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Brewer’s spent grain (BSG) solid residues are constituted by dietary fibre, protein, sugars, and polyphenols, which can have potential effects on human health. In this study, for the first time, the flours obtained from solid residues of solid-liquid extraction (SLE) and ohmic heating extraction (OHE) were applied throughout the gastrointestinal di...
Article
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The impact of nanotechnology on the exponential growth of several research areas, particularly nanomedicine, is undeniable. The ability to deliver active molecules to the desired site could significantly improve the efficiency of medical treatments. One of the nanocarriers developed which has drawn researchers’ attention are cubosomes, which are na...
Article
Microbial biosurfactants have attracted the attention of researchers and companies for the last decades, as they are considered promising candidates to replace chemical surfactants in numerous applications. Although in the last years, considerable advances were performed regarding strain engineering and the use of low-cost substrates in order to re...
Article
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Metallic and bimetallic nanostructures have shown interesting chromatic and antibacterial properties, and they can be used in various applications. In this work, zinc (Zn) and iron (Fe) nanostructures were produced with different morphologies: (i) pure Zn; (ii) Zn-Fe nanoalloys; (iii) Zn-Fe nanolayers (Zn-Fe NLs); and (iv) Zn nanolayers combined wi...
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Fructooligosaccharides (FOS) are usually synthesized with pure enzymes using highly concentrated sucrose solutions. In this work, low-cost aguamiel and molasses were explored as sucrose alternatives to produce FOS, via whole-cell fermentation, with an Aspergillus oryzae DIA-MF strain. FOS production process was optimized through a central composite...
Article
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Research with fermented olives as a source of wild Lactic Acid Bacteria (LAB) strains with probiotic and biotechnological characteristics constitutes a promising field of work. The present study evaluated in vitro probiotic, antifungal, and antimycotoxigenic potential of LAB isolates from naturally fermented Brazilian table olives. Among fourteen L...
Article
The bioavailability of natural compounds should be assessed through different perspectives. Studying the behaviour of the extracts after digestion is often overlooked but is crucial for success in the development of active food ingredients. Thus, the bioaccessibility of S. nigra (flower and berry) extracts after in vitro gastrointestinal digestion...
Article
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This study examined the influence of ohmic heating (OH), compared to the conventional heating (Conven) and Control (solvent) methods, on the extraction of olive mill leaves. The main extraction parameters were: (i) solvent ratio (aqueous ethanol; 40%, 60%, and 80%, v/v), and (ii) extraction temperature; 45 °C, 55 °C, and 75 °C (for OH and Conven),...
Article
Resveratrol is an antioxidant with applications in the food and cosmetic industries. Its biosynthesis can side the hindrances of its extraction from plants. The dairy industry generates tonnes of lactose-rich wastes, which can be a carbon source. Saccharomyces cerevisiae is an industrial workhorse for biotechnological processes, being unable to nat...
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The red seaweed Gelidium sesquipedale is recognized for its high-quality agar, a valued hydrocolloid with gelling capacity, an industrially desirable property strongly dependent on its structure and composition. Despite its importance, there is a lack of information regarding the effects of processing conditions, including alternative extraction te...
Article
The production of phytase and biomass (estimated by the ergosterol content) by submerged fermentation with Aspergillus niger var. phoenicis URM 4924 was studied. Experimental assays were carried under different conditions of pH (4.0 to 8.0) and temperature (25 to 35 ºC), and the influence of these variables on the responses was studied through a 22...
Article
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Green extraction techniques (GreETs) emerged in the last decade as greener and sustainable alternatives to classical sample preparation procedures aiming to improve the selectivity and sensitivity of analytical methods, simultaneously reducing the deleterious side effects of classical extraction techniques (CETs) for both the operator and the envir...
Article
Microalgae are sustainable feedstock for healthy food and feed, organic drugs, ecological polymers, green chemicals and dyes, biofuels, biofertilizers, and environmental bioremediation technologies. Despite its enormous promises, microalgae cultivation is expensive and thus large-scale production is centred on low volume/high value markets, such as...
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Understanding of thermal adaptation mechanisms in yeast is crucial to develop better-adapted strains to industrial processes, providing more economical and sustainable products. We have analyzed the transcriptomic responses of three Saccharomyces cerevisiae strains, a commercial wine strain, ADY5, a laboratory strain, CEN.PK113-7D and a commercial...
Article
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Olive pomace (OP) is the main residue that results from olive oil production. OP is rich in bioactive compounds, including polyphenols, so its use in the treatments of diseases related to oxidative stress, such as cancer, could be considered. The present work aimed to study the biological properties of different OP extracts, obtained by ohmic heati...
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Aloe vera has been medicinally used for centuries. Its bioactive compounds have been shown to be very effective in the treatment of numerous diseases. In this work, a novel functional beverage was developed and characterized to combine the health benefits of probiotic bacteria with the Aloe vera plant itself. Two Aloe vera juices were obtained by f...
Article
The aim of this work was to design and analyse techno–economically and nutritionally the production of nutrient blocks and silage from waste of Dominico–Hartón plantain through process simulation in the framework of the design of a future biorefinery based on residues from plantain agro-industry. All systems were scaled to flows that would be reali...
Article
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A nutrient-rich diet is a key to improving the chemical signals, such as antioxidants, which modulate pathogens’ resistance in the gut and prevent diseases. A current industrial problem is the generation of undervalued by-products, such as tomato bagasse, which are rich in bioactive compounds and of commercial interest (carotenoids and phenolic com...
Article
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The prebiotic potential of fructo-oligosaccharides (microbial-FOS) produced by a newly isolated Aspergillus ibericus, and purified by Saccharomyces cerevisiae YIL162 W, was evaluated. Their chemical structure and functionality were compared to a non-microbial commercial FOS sample. Prebiotics were fermented in vitro by fecal microbiota of five heal...
Article
Within the oral cavity, the ability of Candida species to adhere and form biofilms is well recognized, especially when C. albicans is considered. Lately, a knowledge gap has been identified regarding dual-species communication of Candida isolates, as a way to increase virulence, with evidences being collected to support the existence of interaction...
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Flawless skin is the most universally desired human feature" is an iconic statement by Desmond Morris. Skin indicates one s health and is so important that it affects a person's emotional and psychological behavior, these facts having propelled the development of the cosmetics industry. It is estimated that in 2023, this industry will achieve more...
Article
High-pressure processing (HPP) is an emergent technology for nonthermal pasteurization of foods, which here demonstrates the potential to be used by the chestnut industry. This study evaluated HPP as a post-harvest processing technology to control molds and insect larvae proliferation in chestnuts (Castanea sativa Mill.) and improve the fruit's she...
Article
The aim of this work was to evaluate the production of isomalto–oligosaccharides (IMO) from rejected unripe plantain fruits (Musa AAB Simmonds) on a laboratory scale and assess IMO syrup production for a large scale through process simulation. The enzymatic activity of the commercial preparation of α-glucosidase was 936.23 ± 0.71 U/mL. The Michaeli...
Article
Powdered Pleurotus ostreatus (PO) was tested for the detoxification of ochratoxin A (OTA) and zearalenone (ZEN) through in vitro gastrointestinal digestion in the absence and presence of ground feed and cornmeal. Ochratoxin α was detected in the chromatograms after OTA elimination, suggesting the presence of an OTA-hydrolytic enzyme in the PO, wher...
Chapter
Active packaging refers to a set of systems usually aiming to protect and extend the shelf life of food. The incorporation of metal (e.g. zinc, silver, copper, and gold) and metal oxide (e.g. zinc oxide, titanium dioxide, and magnesium oxide) nanoparticles into petroleum-based and bio-based polymers has progressively increased for active packaging...
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Enzymatic hydrolysis is a key process for lignocellulosic biomass conversion; a way to increase its efficiency and lessen the process cost is by targeting alternative strategies to improve the use of cellulolytic enzymes. This work addresses enzyme immobilization as a strategy to enhance lignocellulosic biomass hydrolysis by simplifying enzyme reco...
Article
Ohmic based brewer's spent grain (BSG) hydro-ethanolic extracts were produced and their composition and biological potential through evaluation of their antibacterial, antibiofilm and antihypertensive activities were characterized. Ohmic Heating Extraction (OHE) was performed using two aqueous extractions of ethanol (60 and 80% ethanol:water (v/v))...
Article
Synthetic colorants have gradually been replaced by natural pigments, mainly in the food industry. Among the several natural sources of pigments, filamentous fungi have gained special attention. However, the eventual biosynthesis of mycotoxins is a limitation for the practical use of this kind of microorganisms as pigment producers. In this work, P...
Article
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Pineapple by-products (peels and stems) from fruit processing industries were evaluated to understand its potential application as a functional food. Therefore, the bioactive compounds of pineapple by-products were characterized for prebiotic and antioxidant activities. A total characterization of soluble carbohydrates profile (simples and complex...
Article
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Edible coatings incorporated with bacteriocin-producing lactic acid bacteria (LAB) can provide an alternative natural preservation method as they can offer an additional microbiological protection to food products. The bacteriocin-producing strains Lactococcus lactis L3A21M1 and Lc. garvieae SJM17 isolated from artisanal Azorean cheeses were incorp...
Chapter
Pulsed electric fields (PEF) is attracting great attention as nonthermal extraction technology on downstream processing of emergent marine bioresources such as micro- and macroalgae. These uni- and multicellular algae enclose a diverse and interesting biochemical composition and are recognized as important sources of valuable bioactive compounds an...
Chapter
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Dietary prebiotics have been associated with the prevention and treatment of many 21st-century diseases. Prebiotics’ health benefits have been linked to microbiome–host complex interactions, modulating microbiota homeostasis, gut–brain axis, microbiota–immune system crosstalk, and the intestine barrier function. There is therefore huge interest fro...
Chapter
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Many public health policies and programs have been implemented in several countries aiming to reduce population sugar consumption and to improve nutritional profile of available processed food. Consumers are also increasingly seeking for nutritional and health-related food able to meet not only nutritional needs, but also improved mental and physic...
Chapter
In the last decade, there was a great effort to promote lignocellulosic biomass (LCB) as a raw material for obtaining new added value compounds. Agricultural and industrial processing activities generate a large volume of by-products, most of which are still little explored, representing a significant risk to the environment. LCB is an abundant and...
Article
The economic impact of different potentially scalable process improvements was here assessed for the first time in the specific context of a Eucalyptus wood biorefinery producing biofuels, high-value chemicals and energy. The base case scenario referring to bioethanol as the only product was clearly unviable, which mostly resulted from the high cos...
Article
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Eggplants contain a multitude of biocompounds with nutritional and/or biological activities. The objective of this work was to study the nutritional, chemical and bioactive value of red eggplant from Rotonda, Italy. Ohmic heating (OH) was compared to conventional heating, as different solvents were used (water, ethanol 30, 50, and 90% and methanol)...
Article
Protein crystallisation has been shown as a viable alternative to conventional protein purification methods. However, the stochastic and spontaneous nature of the primary nucleation process makes protein crystallization difficult to control. An approach often used in the crystallization of small organic and inorganic molecules to improve the reprod...
Article
Correction for ‘Bioactive extracts from brewer's spent grain’ by Teresa Bonifácio-Lopes et al. , Food Funct. , 2020, 11 , 8963–8977, DOI: 10.1039/D0FO01426E.
Article
50 days' free access to this article: https://authors.elsevier.com/a/1eFQb3IS6-X%7Ebq High-pressure processing (HPP) is an emergent technology for non-thermal pasteurization of foods, which here demonstrates the potential to be used by the chestnut industry. This study evaluated HPP as a post-harvest processing technology to control molds and inse...
Article
Full-text available
Gene therapy and DNA vaccination are among the most expected biotechnological and medical advances for the coming years. However, the lack of cost-effective large-scale production and purification of pharmaceutical-grade plasmid DNA (pDNA) still hampers their wide application. Downstream processing, which is mainly chromatography-based, of pDNA rem...
Article
The present paradigm of a new concept of biomass conversion system has been focused on the development of a multi-supply biorefinery concept towards a circular and sustainable bioeconomy. This work was focused on evaluating a mixture of six lignocellulosic biomass types, from forest ecosystems and biological resources from marginal land, in order...
Article
Electric field-based technologies for extraction processes have been gaining importance due to sustainability concerns. This work aims to assess the potential of ohmic heating as an efficient and feasible tool for the extraction of different biocompounds from Gracilaria vermiculophylla and its effect on the extracts' composition. Different ratios o...
Article
The Pleurotus ostreatus mushroom is a source of high nutritional value and bioactive compounds such as β‐glucans, with immunomodulatory, hypoglycemic and prebiotic activity. The aim of this work was to developed a bread with P. ostreatus powder (POP) to increase its nutritional value and reduce its glycemic index. Four formulations were proposed su...
Article
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Natural bioactive compounds from food waste have fomented interest in food and pharmaceutical industries for the past decade. In this work, it purposed the recovery of bioactive avocado peel extract using an environmentally friendly technique: the ultrasound assisted extraction. The response surface methodology was applied in order to optimize the...
Article
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Bee products are a promising source of phenolic compounds with strong antioxidant activity. The present study was designed to explore the protective effect of honey, propolis, and their combination on gentamicin-induced oxidative stress and hepatorenal dysfunction. This study was conducted on male Wistar rats by intraperitoneal injections of gentam...
Article
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The main goal of this study was to chemically characterize an aqueous S. nigra flower extract and validate it as a bioactive agent. The elderflower aqueous extraction was performed at different temperatures (50, 70 and 90 C). The extract obtained at 90 ◦C exhibited the highest phenolic content and antiradical activity. Therefore, this extract was a...
Article
Chitooligosaccharides (COS) have a great potential to be used by pharmaceutical industry due to their many biological activities. The use of enzymes to produce them is very advantageous, however it still faces many challenges, such as discovering new strains capable to produce enzymes that are able to generate bioactive oligosaccharides. In the pre...
Article
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Agave bagasse is a residual biomass in the production of the alcoholic beverage tequila, and therefore, it is a promising raw material in the development of biorefineries using hot compressed water pretreatment (hydrothermal processing). Surfactants application has been frequently reported as an alternative to enhance monomeric sugars production ef...
Article
Bimetallic nanoparticles have gained attention in the last decade due to their unusual characteristics compared to monometallic counterparts. However, production of such particles with controlled morphologies and composition needs to be explored and the mechanisms understood. In this work, we demonstrate a fast and simple process to obtain flower-l...
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FAO estimates that in 2030 the poultry meat production could reach 120 million tons, which is a challenge in terms of waste management. Feathers are mainly composed of keratin, an important biomaterial. Using feathers as a source of keratin will minimize the waste generated, while contributing to supply an important material for several industries,...
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An endophytic bacterium inhibiting pathogenic bacteria was isolated and the strain was genetically identified as Paenibacillus polymyxa. Biochemical characterization of fermentation broth indicated the presence of peptidic antimicrobial molecules. Liquid-liquid partition resulted in an organic fraction (OF) and an aqueous fraction (AF). OF presente...
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Usually, wine-making by-products are discarded, presenting a significant environmental impact. However, they can be used as a source of bioactive compounds. Moreover, consumers’ increasing demand for naturally nutritious and healthy products requires new formulations and food product improvement, together with sustainable, environmentally friendly...
Article
Aflatoxins are hepatotoxic and carcinogenic fungal secondary metabolites that usually contaminate crops and represent a serious health hazard for humans and animals worldwide. In this work, the effect of rhamnolipids (RLs) produced by Pseudomonas aeruginosa #112 on the growth and aflatoxins production by Aspergillus flavus MUM 17.14 was studied in...
Article
In the present study, seven propolis samples collected from different areas of Morocco were evaluated for various potential attributes. Physicochemical parameters (moisture, pH, soluble substance, insoluble substance, ash content, conductivity, organic matter, resin, balsams, total carbohydrates, total proteins and mineral content), structural char...
Article
Exosomes are cell-derived vesicles that act as carriers for proteins and nucleic acids, with therapeutic potential and high biocompatibility. We propose a new concept of exosome-like liposomes for controlled delivery. The goal of this work was to develop a new type of liposomes with a unique mixture of phospholipids, similar to naturally occurring...
Article
Agro-food industries generate a huge amount of fruit and vegetable wastes. These residues are composed by structural parts (leaves, peels, pulps, seeds, roots and stems) after the extraction of their juices, and they are rich in bioactive compounds such polyphenols, protein, carbohydates, fibre, lignin, lipids and minerals. Therefore, these wastes...
Chapter
The contamination of foodstuffs and agricultural commodities by mycotoxin‐producing fungi is a challenging problem. Mycotoxins are secondary metabolites synthesized by several filamentous fungi that exhibit carcinogenic, mutagenic and teratogenic activities even at low concentrations. Besides their toxicity, mycotoxins are also responsible for subs...
Chapter
Despite the significant efforts to expand the use of renewable energy sources, our society is still highly dependent on crude oil. The progressive exhaustion of light crude oil reserves forced the oil companies to develop alternative strategies to recover the entrapped oil remaining in the reservoirs. These technologies, known as Enhanced Oil Recov...
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Lignocellulosic residues have been receiving growing interest as a promising source of polysaccharides, which can be converted into a variety of compounds, ranging from biofuels to bioplastics. Most of these can replace equivalent products traditionally originated from petroleum, hence representing an important environmental advantage. Lignocellulo...