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  • José A Teixeira
José A Teixeira

José A Teixeira
University of Minho · Institute for Biotechnology and Bioengineering - Centre of Biological Engineering

About

924
Publications
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36,275
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Publications

Publications (924)
Article
Metallic and bimetallic nanostructures have shown interesting chromatic and antibacterial properties, and they can be used in various applications. In this work, zinc (Zn) and iron (Fe) nanostructures were produced with different morphologies: (i) pure Zn; (ii) Zn-Fe nanoalloys; (iii) Zn-Fe nanolayers (Zn-Fe NLs); and (iv) Zn nanolayers combined wi...
Article
Full-text available
Fructooligosaccharides (FOS) are usually synthesized with pure enzymes using highly concentrated sucrose solutions. In this work, low-cost aguamiel and molasses were explored as sucrose alternatives to produce FOS, via whole-cell fermentation, with an Aspergillus oryzae DIA-MF strain. FOS production process was optimized through a central composite...
Article
The bioavailability of natural compounds should be assessed through different perspectives. Studying the behaviour of the extracts after digestion is often overlooked but is crucial for success in the development of active food ingredients. Thus, the bioaccessibility of S. nigra (flower and berry) extracts after in vitro gastrointestinal digestion...
Article
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This study examined the influence of ohmic heating (OH), compared to the conventional heating (Conven) and Control (solvent) methods, on the extraction of olive mill leaves. The main extraction parameters were: (i) solvent ratio (aqueous ethanol; 40%, 60%, and 80%, v/v), and (ii) extraction temperature; 45 °C, 55 °C, and 75 °C (for OH and Conven),...
Article
Resveratrol is an antioxidant with applications in the food and cosmetic industries. Its biosynthesis can side the hindrances of its extraction from plants. The dairy industry generates tonnes of lactose-rich wastes, which can be a carbon source. Saccharomyces cerevisiae is an industrial workhorse for biotechnological processes, being unable to nat...
Article
The red seaweed Gelidium sesquipedale is recognized for its high-quality agar, a valued hydrocolloid with gelling capacity, an industrially desirable property strongly dependent on its structure and composition. Despite its importance, there is a lack of information regarding the effects of processing conditions, including alternative extraction te...
Article
Full-text available
Understanding of thermal adaptation mechanisms in yeast is crucial to develop better-adapted strains to industrial processes, providing more economical and sustainable products. We have analyzed the transcriptomic responses of three Saccharomyces cerevisiae strains, a commercial wine strain, ADY5, a laboratory strain, CEN.PK113-7D and a commercial...
Article
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Recently, there are two main movements gaining public attention: a “green revolution” focused on the use of sustainable processes and the increasing concerns with health-promoting diets and the recognition of the gut microbiota and its effects on the treatment and prevention of several diseases. Microalgae and macroalgae arise as an interesting alt...
Article
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Olive pomace (OP) is the main residue that results from olive oil production. OP is rich in bioactive compounds, including polyphenols, so its use in the treatments of diseases related to oxidative stress, such as cancer, could be considered. The present work aimed to study the biological properties of different OP extracts, obtained by ohmic heati...
Article
The aim of this work was to design and analyse techno–economically and nutritionally the production of nutrient blocks and silage from waste of Dominico–Hartón plantain through process simulation in the framework of the design of a future biorefinery based on residues from plantain agro-industry. All systems were scaled to flows that would be reali...
Article
Full-text available
A nutrient-rich diet is a key to improving the chemical signals, such as antioxidants, which modulate pathogens’ resistance in the gut and prevent diseases. A current industrial problem is the generation of undervalued by-products, such as tomato bagasse, which are rich in bioactive compounds and of commercial interest (carotenoids and phenolic com...
Article
Full-text available
The prebiotic potential of fructo-oligosaccharides (microbial-FOS) produced by a newly isolated Aspergillus ibericus, and purified by Saccharomyces cerevisiae YIL162 W, was evaluated. Their chemical structure and functionality were compared to a non-microbial commercial FOS sample. Prebiotics were fermented in vitro by fecal microbiota of five heal...
Article
Within the oral cavity, the ability of Candida species to adhere and form biofilms is well recognized, especially when C. albicans is considered. Lately, a knowledge gap has been identified regarding dual-species communication of Candida isolates, as a way to increase virulence, with evidences being collected to support the existence of interaction...
Article
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Flawless skin is the most universally desired human feature" is an iconic statement by Desmond Morris. Skin indicates one s health and is so important that it affects a person's emotional and psychological behavior, these facts having propelled the development of the cosmetics industry. It is estimated that in 2023, this industry will achieve more...
Article
High-pressure processing (HPP) is an emergent technology for nonthermal pasteurization of foods, which here demonstrates the potential to be used by the chestnut industry. This study evaluated HPP as a post-harvest processing technology to control molds and insect larvae proliferation in chestnuts (Castanea sativa Mill.) and improve the fruit's she...
Article
The aim of this work was to evaluate the production of isomalto–oligosaccharides (IMO) from rejected unripe plantain fruits (Musa AAB Simmonds) on a laboratory scale and assess IMO syrup production for a large scale through process simulation. The enzymatic activity of the commercial preparation of α-glucosidase was 936.23 ± 0.71 U/mL. The Michaeli...
Article
Powdered Pleurotus ostreatus (PO) was tested for the detoxification of ochratoxin A (OTA) and zearalenone (ZEN) through in vitro gastrointestinal digestion in the absence and presence of ground feed and cornmeal. Ochratoxin α was detected in the chromatograms after OTA elimination, suggesting the presence of an OTA-hydrolytic enzyme in the PO, wher...
Chapter
Active packaging refers to a set of systems usually aiming to protect and extend the shelf life of food. The incorporation of metal (e.g. zinc, silver, copper, and gold) and metal oxide (e.g. zinc oxide, titanium dioxide, and magnesium oxide) nanoparticles into petroleum-based and bio-based polymers has progressively increased for active packaging...
Article
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Enzymatic hydrolysis is a key process for lignocellulosic biomass conversion; a way to increase its efficiency and lessen the process cost is by targeting alternative strategies to improve the use of cellulolytic enzymes. This work addresses enzyme immobilization as a strategy to enhance lignocellulosic biomass hydrolysis by simplifying enzyme reco...
Article
Ohmic based brewer's spent grain (BSG) hydro-ethanolic extracts were produced and their composition and biological potential through evaluation of their antibacterial, antibiofilm and antihypertensive activities were characterized. Ohmic Heating Extraction (OHE) was performed using two aqueous extractions of ethanol (60 and 80% ethanol:water (v/v))...
Article
Synthetic colorants have gradually been replaced by natural pigments, mainly in the food industry. Among the several natural sources of pigments, filamentous fungi have gained special attention. However, the eventual biosynthesis of mycotoxins is a limitation for the practical use of this kind of microorganisms as pigment producers. In this work, P...
Article
Full-text available
Pineapple by-products (peels and stems) from fruit processing industries were evaluated to understand its potential application as a functional food. Therefore, the bioactive compounds of pineapple by-products were characterized for prebiotic and antioxidant activities. A total characterization of soluble carbohydrates profile (simples and complex...
Chapter
Pulsed electric fields (PEF) is attracting great attention as nonthermal extraction technology on downstream processing of emergent marine bioresources such as micro- and macroalgae. These uni- and multicellular algae enclose a diverse and interesting biochemical composition and are recognized as important sources of valuable bioactive compounds an...
Chapter
In the last decade, there was a great effort to promote lignocellulosic biomass (LCB) as a raw material for obtaining new added value compounds. Agricultural and industrial processing activities generate a large volume of by-products, most of which are still little explored, representing a significant risk to the environment. LCB is an abundant and...
Article
The economic impact of different potentially scalable process improvements was here assessed for the first time in the specific context of a Eucalyptus wood biorefinery producing biofuels, high-value chemicals and energy. The base case scenario referring to bioethanol as the only product was clearly unviable, which mostly resulted from the high cos...
Article
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Eggplants contain a multitude of biocompounds with nutritional and/or biological activities. The objective of this work was to study the nutritional, chemical and bioactive value of red eggplant from Rotonda, Italy. Ohmic heating (OH) was compared to conventional heating, as different solvents were used (water, ethanol 30, 50, and 90% and methanol)...
Article
Protein crystallisation has been shown as a viable alternative to conventional protein purification methods. However, the stochastic and spontaneous nature of the primary nucleation process makes protein crystallization difficult to control. An approach often used in the crystallization of small organic and inorganic molecules to improve the reprod...
Article
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Gene therapy and DNA vaccination are among the most expected biotechnological and medical advances for the coming years. However, the lack of cost-effective large-scale production and purification of pharmaceutical-grade plasmid DNA (pDNA) still hampers their wide application. Downstream processing, which is mainly chromatography-based, of pDNA rem...
Article
The present paradigm of a new concept of biomass conversion system has been focused on the development of a multi-supply biorefinery concept towards a circular and sustainable bioeconomy. This work was focused on evaluating a mixture of six lignocellulosic biomass types, from forest ecosystems and biological resources from marginal land, in order...
Article
Electric field-based technologies for extraction processes have been gaining importance due to sustainability concerns. This work aims to assess the potential of ohmic heating as an efficient and feasible tool for the extraction of different biocompounds from Gracilaria vermiculophylla and its effect on the extracts' composition. Different ratios o...
Article
Full-text available
Agave bagasse is a residual biomass in the production of the alcoholic beverage tequila, and therefore, it is a promising raw material in the development of biorefineries using hot compressed water pretreatment (hydrothermal processing). Surfactants application has been frequently reported as an alternative to enhance monomeric sugars production ef...
Article
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FAO estimates that in 2030 the poultry meat production could reach 120 million tons, which is a challenge in terms of waste management. Feathers are mainly composed of keratin, an important biomaterial. Using feathers as a source of keratin will minimize the waste generated, while contributing to supply an important material for several industries,...
Article
Aflatoxins are hepatotoxic and carcinogenic fungal secondary metabolites that usually contaminate crops and represent a serious health hazard for humans and animals worldwide. In this work, the effect of rhamnolipids (RLs) produced by Pseudomonas aeruginosa #112 on the growth and aflatoxins production by Aspergillus flavus MUM 17.14 was studied in...
Article
Exosomes are cell-derived vesicles that act as carriers for proteins and nucleic acids, with therapeutic potential and high biocompatibility. We propose a new concept of exosome-like liposomes for controlled delivery. The goal of this work was to develop a new type of liposomes with a unique mixture of phospholipids, similar to naturally occurring...
Article
Agro-food industries generate a huge amount of fruit and vegetable wastes. These residues are composed by structural parts (leaves, peels, pulps, seeds, roots and stems) after the extraction of their juices, and they are rich in bioactive compounds such polyphenols, protein, carbohydates, fibre, lignin, lipids and minerals. Therefore, these wastes...
Chapter
The contamination of foodstuffs and agricultural commodities by mycotoxin‐producing fungi is a challenging problem. Mycotoxins are secondary metabolites synthesized by several filamentous fungi that exhibit carcinogenic, mutagenic and teratogenic activities even at low concentrations. Besides their toxicity, mycotoxins are also responsible for subs...
Chapter
Despite the significant efforts to expand the use of renewable energy sources, our society is still highly dependent on crude oil. The progressive exhaustion of light crude oil reserves forced the oil companies to develop alternative strategies to recover the entrapped oil remaining in the reservoirs. These technologies, known as Enhanced Oil Recov...
Article
Full-text available
Lignocellulosic residues have been receiving growing interest as a promising source of polysaccharides, which can be converted into a variety of compounds, ranging from biofuels to bioplastics. Most of these can replace equivalent products traditionally originated from petroleum, hence representing an important environmental advantage. Lignocellulo...
Article
Full-text available
Ohmic heating (OH) was evaluated as a post-harvest technology to improve chestnuts’ shelf-life (Castanea sativa Mill.) by controlling molds and insect larvae proliferation. Chestnuts were processed by OH at 35, 45, and 55 °C and compared with untreated fruits and the conventional hydrothermal technology (HT - 50 °C for 45 min), which is the process...
Article
A sequential multi-stage procedure was applied on the extraction of biocompounds from Spirulina platensis. The process consisted in three steps: 1) aqueous extraction, using conventional thermal extraction (CE), ohmic heating (OH, 7 V/cm), enzymatic treatment (EAE, 0.8 mgLysozyme/mL), or both OH and EAE combined; 2) ethanolic extraction; 3) CHCl3/M...
Article
Ohmic heating (OH) encompasses interesting benefits towards thermal processing. Envisaging an increasing relevance of soybean protein as an alternative non-animal protein, it is important to understand how OH can contribute to the quality and immunoreactivity of soybean-derived products. This study describes, for the first time, the impact of OH wh...
Article
Animal-based proteins are the most consumed worldwide given their well-balanced nutritional composition. However, the growing demand for animal proteins will not be sustainable due to their low conversion efficiency and high environmental footprint. Specific consumers’ dietary restrictions and modern trends emphasize the importance of finding alter...
Article
The Attenuated Total Reflection – Fourier Transform Infrared (ATR-FTIR) spectra of the OH-stretch band in aqueous solutions of inorganic salts and organic materials; Na2SO4, NaCl, NaClO4, NaSCN, trimethylamine N-oxide, urea, poly(ethylene glycol), polyvinylpyrrolidone, and copolymer of ethylene glycol and propylene glycol (Ucon) were studied at var...
Article
Full-text available
Seaweeds are a renewable natural source of valuable macro and micronutrients that have attracted the attention of the scientists in the last years. Their medicinal properties were already recognized in the ancient traditional Chinese medicine, but only recently there has been a considerable increase in the study of these organisms in attempts to de...
Article
The increasing consumption of plantain fruits with specific quality standards generates high agricultural waste. This work aimed at valorising the rejected unripe pulp of DominicoHartón plantain fruits (Musa AAB Simmonds). The pulp was characterised physicochemically, thermally and functionally. The data gathered experimentally and collected from d...
Article
Full-text available
Polyphenolic extracts from pine bark have reported different biological actions and promising beneficial effects on human health. However, its susceptibility to environmental stresses (temperature, storage, etc.) and physiological human conditions prequires the development of efficient protection mechanisms to allow effective delivering of function...
Article
In this work, hydrothermal treatment was assessed for the fractionation of industrial Eucalyptus globulus bark residue (EBR) to obtain biofuels and value-added compounds (such as oligosaccharides and phenolic compounds) in separated streams. Hydrothermal treatment was evaluated under non-isothermal regimen in the range of maximum temperature (Tmax)...
Article
There is a current demand for novel active food packaging solutions using biodegradable materials and no chemical antimicrobial compounds, to ensure food quality and safety. This work involved the incorporation of Salmonella Enteritidis bacteriophage Felix O1, for potential use as an anti-Salmonella agent, into polyvinyl alcohol (PVOH) coatings and...
Article
Full-text available
Fabric structures are prone to contamination with microorganisms, as their morphology and ability to retain moisture creates a proper environment for their growth. In this work, a novel, easily processed and cheap coating for a nylon fabric with antimicrobial characteristics was developed. After plasma treatment, made to render the fabric surface m...
Article
Bio-based rhamnolipid production from waste streams is gaining momentum nowadays because of increasing market demand, huge range of applications and its economic and environment friendly nature. Rhamnolipid type biosurfactants are produced by microorganisms as secondary metabolites and have been used to reduce surface/interfacial tension between tw...
Article
Full-text available
Elucidation of temperature tolerance mechanisms in yeast is essential for enhancing cellular robustness of strains, providing more economically and sustainable processes. We investigated the differential responses of three distinct Saccharomyces cerevisiae strains, an industrial wine strain, ADY5, a laboratory strain, CEN.PK113-7D and an industrial...
Article
Background The current circular economy system-based sustainability and the social lifestyle trends, have led to a developed structure that is restorative or regenerative by purpose and innovation. It substitutes the end-of-life idea of a by-product adding value to it, shifts towards the usage of environmentally friendly solutions, eliminating the...
Article
Fibers from passion fruit stalks were first recovered with a pulping and bleaching process. Two mechanical treatments were further applied to the fibers, homogenization (with and without ultrasound), and blender application. The effect of those treatments on fibers was evaluated. The chemical composition of the different stages of fibers undergoing...
Article
Seaweeds are promising feedstocks; nevertheless, the lack of systematic approaches to recover different high-value fractions in a clean and sustainable mode hampers their exploitation. Due to this necessity, an innovative environmentally friendly strategy was proposed in this article for the development of a sugar platform from Gelidium sesquipedal...
Article
The antioxidant activity of baked foods is of utmost interest when envisioning enhancing their health benefits. Incorporating functional ingredients is challenging since their bioactivity naturally declines during baking. In this study, 3D food printing and design of experiments are employed to clarify how the antioxidant activity of cookies enrich...
Conference Paper
Full-text available
Introduction. The generation of agricultural waste, in particular from plantain fruit, have been increasing. Its accumulation and poor valorization limits the environmental, economic and social development of communities. The main goal of this work was to characterize physicochemically and functionally the flour obtained from rejected Dominico–Hart...
Article
Full-text available
Fibrinolytic enzymes with a direct mechanism of action and safer properties are currently requested for thrombolytic therapy. This paper reports on a new enzyme capable of degrading blood clots directly without impairing blood coagulation. This enzyme is also non-cytotoxic and constitutes an alternative to other thrombolytic enzymes known to cause...
Article
This study aims to obtain aqueous extracts of valuable phytochemicals, in particular anthocyanin’s, from winemaking residues by using grape skins as natural electrical conductors allowing internal heat dissipation through Ohmic Heating (OH) effect. Two different electric pretreatments were evaluated: i) using mild temperatures at 40 °C during 20 mi...
Article
All coffee brews are prepared with roasted coffee and water, giving origin to espresso, instant, or filtered coffee, exhibiting distinct physicochemical properties, depending on the extraction conditions. The different relative content of compounds in the brews modulates coffee body, aroma, and colour. In this study it was hypothesized that a coffe...
Article
In this work rhamnolipids were evaluated as surfactants for the production of nanostructured lipid carriers (NLCs). NLCs were produced by melt-emulsification using ultra-homogenisation followed by ultrasonication and different ratios of medium-chain-triglycerides and glycerol monostearate (lipid phase) were tested. NLCs presented sizes and polydisp...
Preprint
Full-text available
The antioxidant activity of baked foods is of utmost interest when envisioning enhancing their health benefits. Incorporating functional ingredients is challenging since their bioactivity naturally declines during baking. In this study, 3D food printing and design of experiments are employed to clarify how the antioxidant activity of cookies enrich...
Article
The present study was designed to investigate the preventive effect of propolis, bee pollen and their combination on Type 2 diabetes induced by D-glucose in rats. The study was carried out by feeding daily two concentrations (100 and 200 mg/Kg BW) of propolis or bee pollen (or their combination to normal (non-diabetic) and diabetic rats for a perio...
Article
In this study, antioxidant-rich extracts from brewer's spent grain (BSG) extracted by solid-to-liquid extraction using different solvents water and ethanol and their mixtures at two ratios (80% ethanol : water (v/v) and 60% ethanol : water (v/v)) were characterized. Nutritional composition was evaluated for the extracts and for the solid residues o...
Article
Full-text available
Superabsorbent hydrogels (SAHs) are three dimensional networks formed by polymers that can absorb aqueous solution of over 100% of their initial weight. This work aimed to develop and characterize SAHs of Chitosan/Xanthan gum (CG), Chitosan/Alginate (CA) and controlled Chitosan (C), Xanthan gum (G), and Alginate (A) produced using "onion-like" meth...
Article
Lignin particles (LPs) have gained prominence due to their biodegradability and bioactive properties. LP production at nano and micro scale produced from organosolv lignin and the understanding of size's effect on their properties is unexplored. This work aimed to produce and characterize lignin nanoparticles and microparticles using a green synthe...
Article
Full-text available
The agricultural and processing activities of olive crops generate a substantial amount of food by-products, particularly olive leaves, which are mostly underexploited, representing a significant threat to the environment. Olive leaves are endowed with endogenous bioactive compounds. Their beneficial/health-promoting potential, together with enviro...
Conference Paper
Sustainable processes are required to decrease the environmental and economic impact of residues and wastes. Therefore, the agro-industrial waste upcycling, as substitutes of substrate, is of paramount relevance. In this work, the nutritional value of silage from fresh residues of plantain agroindustry was evaluated by process simulation, as an alt...