José Celso RochaSão Paulo State University | Unesp · Department of Biological Sciences
José Celso Rocha
PhD
About
51
Publications
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Publications
Publications (51)
Study question
Is it possible to estimate blastocyst ploidy with an artificial intelligence (AI) algorithm built using embryo morphokinetics data?
Summary answer
AI was able to estimate blastocysts status in euploid or aneuploid with an accuracy of 0.99 (training) and 0.71 and 0.70 AUC (blind-test), respectively.
What is known already
Morphokinet...
Despite the use of new techniques on embryo selection and the presence of equipment on the market, such as EmbryoScope® and Geri®, which help in the evaluation of embryo quality, there is still a subjectivity between the embryologist’s classifications, which are subjected to inter- and intra-observer variability, therefore compromising the successf...
OBJECTIVE
To study the application of image processing for segmentation of blastocysts images and extraction of potential variables for prediction of embryo fitness.
DESIGN
Retrospective study.
SETTING
Single reproductive medical center. IVI-RMA (Valencia, Spain) between 2017 and 2019.
PATIENTS
An initial dataset including 353 images from Embryo...
Study question
Is the blastocyst morphology image analysis combined with the protein content of spent embryo culture medium a suitable way to predict embryo ploidy?
Summary answer
Morphological variables from blastocyst image analysis combined with IL-6 or MMP-1 concentration in spent culture medium showed more than 80% of accuracy for euploidy pr...
Study question
Does the post-warmed blastocyst dynamics have an impact over the likelihood of achieving a live birth?
Summary answer
Variables related to dynamics of vitrified/warmed blastocysts have shown a greater effect on the live birth prediction than only embryo morphological quality through artificial intelligence.
What is known already
Mo...
An artificial intelligence model was designed using proteomic and morphological data from blastocysts. The algorithm, based on artificial neural networks, is capable of discriminating with high accuracy between euploid embryos that lead to a live birth and those which do not. ABSTRACT Research question: The study aimed to develop an artificial inte...
INPI CARTA PATENTE Nº BR 102012031953-5 O INSTITUTO NACIONAL DA PROPRIEDADE INDUSTRIAL concede a presente PATENTE DE INVENÇÃO, que outorga ao seu titular a propriedade da invenção caracterizada neste título, em todo o território nacional, garantindo os direitos dela decorrentes, previstos na legislação em vigor. (21) Número do Depósito: BR
Research Question
To develop an Artificial Intelligence (AI) model based on Artificial Neural Networks (ANNs) to predict the likelihood of achieving a live birth (LB) by using the proteomic profile of spent culture media and morphological data from time-lapse images.
Design
This retrospective cohort study included 212 patients who underwent single...
Over the past years, the assisted reproductive technologies (ARTs) have been accompanied by constant innovations. For instance, intracytoplasmic sperm injection (ICSI), time-lapse monitoring of the embryonic morphokinetics, and PGS are innovative techniques that increased the success of the ART. In the same trend, the use of artificial intelligence...
Based on growing demand for assisted reproduction technology, improved predictive models are required to optimize in vitro fertilization/intracytoplasmatic sperm injection strategies, prioritizing single embryo transfer. There are still several obstacles to overcome for the purpose of improving assisted reproductive success, such as intra-and inter...
We develop an online graphical and intuitive interface connected to a server aiming to facilitate access to professionals worldwide that face problems with bovine blastocysts classification. The interface Blasto3Q (3Q is referred to the three qualities of the blastocyst grading) contains a description of 24 variables that are extracted from the ima...
The beer quality can be modulated from changes in their ingredient proportions, as well as in operating parameters. The crossed experimental designs and the multiple optimizations based on desirability functions have demonstrated to be effective methodologies in the unit operation polynomial modeling and optimization of bioprocess, respectively. Ho...
In this study, we developed an online graphical and intuitive interface connected to a server aiming to facilitate professional access worldwide to those facing problems with bovine blastocysts classification. The interface Blasto3Q, where 3Q refers to the three qualities of the blastocyst grading, contains a description of 24 variables that were e...
Abstracts of the
34th Annual Meeting of the
European Society of
Human Reproduction and Embryology
We develop an online graphical and intuitive interface connected to a server aiming to facilitate access to professionals worldwide that face problems with bovine blastocysts classification. The interface Blasto3Q (3Q is referred to the three qualities of the blastocyst grading) contains a description of 24 variables that are extracted from the ima...
We develop an online graphical and intuitive interface connected to a server aiming to facilitate access to professionals worldwide that face problems with bovine blastocysts classification. The interface Blasto3Q (3Q is referred to the three qualities of the blastocyst grading) contains a description of 24 variables that are extracted from the ima...
The water quality index (WQI) is an important tool for water resource management and planning. However, it has major disadvantages: the generation of chemical waste, is costly, and time-consuming. In order to overcome these drawbacks, we propose to simplify this index determination by replacing traditional analytical methods with ultraviolet-visibl...
There is currently no objective, real-time and non-invasive method for evaluating the quality of mammalian embryos. In this study, we processed images of in vitro produced bovine blastocysts to obtain a deeper comprehension of the embryonic morphological aspects that are related to the standard evaluation of blastocysts. Information was extracted f...
Morphological analysis is the standard method of assessing embryo quality; however, its inherent subjectivity tends to generate discrepancies among evaluators. Using genetic algorithms and artificial neural networks (ANNs), we developed a new method for embryo analysis that is more robust and reliable than standard methods. Bovine blastocysts produ...
One of the major challenges in stream ecology is the development of computational models that can predict aspects of the community structure of organisms from these ecosystems when they are subject to natural or artificial environmental fluctuations. To contribute towards this aim, we conducted a study whose main goal was to evaluate the efficiency...
This work aimed to determinate eight beer properties using UV-Vis spectrums in combination with principal component regression (PCR) or artificial neural network (ANN) models. A statistical experimental design was performed to generate the calibration data. Firstly, principal component analysis was applied to original spectral data, the scores in s...
The conflict between humans and wild carnivores due to predation on livestock has been one reason that made the mammalian Order Carnivora as one of the groups with most threatened species. In Brazil, the predation by pumas has been recorded in various regions and results in harsh persecution on these animals. The primary goal of this study of ours...
Morphological embryo classification is of great importance for many laboratory techniques, from basic research to the ones applied to assisted reproductive technology. However, the standard classification method for both human and cattle embryos, is based on quality parameters that reflect the overall morphological quality of the embryo in cattle,...
This work aimed to establish a chemometric technique for quantifying amylase and protease activities as well as protein concentration in aqueous extracts of Rhizopus microsporus var. oligosporus obtained via solid-state fermentation (SSF). The kinetics of four agro-industrial wastes (wheat bran, soybean meal, type II wheat flour and sugarcane bagas...
This work objective was to define a modeling approach based on genetic algorithm (GA) for optimizing parameters of an artificial neural network (ANN); the latter describes rabies virus production in BHK-21 cells based on empirical data derived from uniform designs (UDs) with different numbers of experimental runs. The parameters considered for vira...
Currently, mammalian cells are the most utilized hosts for biopharmaceutical production. The culture media for these cell lines include commonly in their composition a pH indicator. Spectroscopic techniques are used for biopharmaceutical process monitoring, among them, UV-Vis spectroscopy has found scarce applications. This work aimed to define art...
This work aimed to compare the predictive capacity of empirical models, based on the uniform design utilization combined to artificial neural networks with respect to classical factorial designs in bioprocess, using as example the rabies virus replication in BHK-21 cells. The viral infection process parameters under study were temperature (34, 37 o...
Embryonic morphological classification has great importance for numerous laboratory techniques (from basic to applied research in assisted reproduction). However, the method used to perform the classification of embryos in varying degrees of quality has always been based on the subjectivity of the evaluator. Although quality standards and descripti...
Morphologically classifying embryos is important for numerous laboratory techniques, which range from basic methods to methods for assisted reproduction. However, the standard method currently used for classification is subjective and depends on an embryologist’s prior training. Thus, our work was aimed at developing software to classify morphologi...
Objective: This study aimed to build an artificial neural network to help the managers of university cafeterias to predict the number of daily meals. Methods: This study was based on a survey of eight variables that influence the number of daily meals served by a university cafeteria. Backpropagation training algorithm was used and the results obta...
AIM: This study was carried out to assess longitudinal and temporal distribution and the effects of organic pollution on macroalgal communities in a subtropical stream; METHODS: The occurrence and percent cover of stream macroalgae were investigated during seasonal period in four sampling sites along the course of the Cascavel Stream, in Paraná Sta...
AIM: This study was carried out to assess longitudinal and temporal distribution and the effects of organic pollution on macroalgal communities in a subtropical stream; METHODS: The occurrence and percent cover of stream macroalgae were investigated during seasonal period in four sampling sites along the course of the Cascavel Stream, in Paraná Sta...
O controle de flutter é um problema cuja solução é almejada ao longo de décadas e que ainda se apresenta como um desafio considerável. Os desafios residem basicamente no projeto de dispositivos de atuação eficientes e na síntese das leis de controle. A lógica difusa se mostra como uma técnica promissora e efetiva de controle. Neste trabalho é propo...
Projects
Projects (3)
Despite several observational studies have produced important contributions to knowledge of stream macroalgal ecology, gaps still remain about the relationship of the environment and ecological distribution patterns of these organisms. As an alternative to improve the understanding of the ecology of these algae, experimental investigations began to be conducted in Brazil. Exactly in experimental ecology of tropical stream macroalgae that this research intends to make its main contributions. In this context, two experimental studies are being proposed in order to assess (1) the influences of substrate complexity, hydraulic conditions and physical disturbances and (2) the effects of anthropogenic substances on the establishment and development of stream macroalgae. In the first study, experiments have been conducted in order to simulate the occurrence of physical disturbances on artificial substrates with five different levels of structural complexity and specific hydraulic conditions. The effects of these variables will be assessed considering as response variables richness (number of species) and abundance (percent cover and chlorophyll a content) of macroalgae. In the second study, laboratorial experiments have been conducted aiming evaluate the effects of different concentrations of glyphosate herbicides on photosynthetic responses of macroalgal species. Photosynthetic responses are assessed by two techniques: (i) chlorophyll fluorescence and (ii) dissolved oxygen evolution.
In this Project we investigate the reasons and possible solutions to the conflict of feline top predators and livestock
