Jose Manuel Moreno-Rojas

Andalusian Institute of Agricultural and Fisheries Research and Training (IFAPA) Cordoba, Spain · Food Science and Health

Keywords: sweet pepper varieties, organic and conventional farming, irrigation regime, antioxidant activity, polyphenols, lycopene and b-carotene, tannins

Carob pulp is a Mediterranean by-product highlighted as an important source of bioactive compounds potentially used in different industrial applications. This paper deals with the comparison of methods elaborated with combinations of conventional (maceration-M, organic solvents and water) and non-conventional green (ultrasound-assisted extraction -...

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The objective of this study was to investigate the impact of nutrient starvation on the growth, biochemical, metabolomic, and biological traits of Lavandula viridis L'Hér and Thymus lotocephalus G. López and R. Morales in vitro cultures. In both species, a reduction in shoot growth and in the production of chlorophyll and carotenoids was observed i...

The objective of this study was to analyze the chemical profile of extracts from different fruit parts of the perennial arborescent cactus Opuntia leucotricha by applying ultra-high-performance liquid chromatography–high-resolution mass spectrometry (UHPLC-HRMS) and to evaluate their biological properties (antioxidant and enzyme inhibitory capaciti...

Climate change is affecting all regions of the world, and the Mediterranean region is one of the most affected. Plants accumulate secondary metabolites as an adaptive response to stress circumstances. The present study investigated the effect of different abiotic factor conditions (drought, moderate heat, severe heat, salinity, and UV-B radiation)...

Pigments, mainly anthocyanins and carotenoids, are important contributors to fruits' visual appearance and nutritional properties. In strawberry (Fragaria spp.), the genetic and molecular mechanisms regulating fruit carotenoid biosynthesis and its natural variation remain largely unexplored. In this study, we sought to identify genomic loci contrib...

The global demand for organic food products has rapidly increased over the last years, becoming an emerging niche market targeting the high-income segment. The higher retailing price for organic food products may increase the risk of fraudulent practices at the different stages of the food supply chain, and consequently, substantial control is need...

Black carrot (Daucus carota ssp. sativus var. atrorubens Alef.) is widely recognized for its bioactive compounds and antioxidant properties. The black carrot of Cuevas Bajas (Málaga) is a local variety characterized by a black/purple core, which differs from other black carrot varieties. Therefore, this autochthonous variety was characterized accor...

This study investigated the impact of in vivo available colon-mango (poly)phenols on stress-induced impairment of intestinal barrier function. Caco-2/HT29-MTX cells were incubated with six extracts of ileal fluid collected pre- and 4-8 h post-mango consumption before being subjected to inflammatory stress. (Poly)phenols in ileal fluids were analyse...

The present work was conducted to evaluate the volatile profile of Ecuadorian Forastero, CCN-51, ETT103 and LR14 cocoa beans during traditional fermentation in laurel wood boxes followed by a sun-drying process. Fifty-six volatiles were identified with HS-SPME-GC-MS. Aldehydes, alcohols and ketones were the compounds that mainly characterized the f...

The banana is a tropical fruit characterized by its composition of healthy and nutritional compounds. This fruit is part of traditional Ecuadorian gastronomy, being consumed in a wide variety of ways. In this context, unripe Red Dacca banana samples and those submitted to different traditional Ecuadorian heating treatments (boiling, roasting, and b...

Isotopic analysis of carbon and nitrogen in faeces is a reliable methodology for studying ecology in wildlife. Here, we tested this technique to detect variations in carbon and nitrogen isotopic ratios (δ13C and δ15N) in two different intrasexual competition scenarios of male Iberian red deer (Cervus elaphus hispanicus) using faeces of individuals...

After an acute intake of 300 g of mango purée by 10 subjects, 0 and 24 h urine and plasma samples were analyzed by high-performance liquid chromatography-high-resolution mass spectrometry. The method was first validated for 44 reference polyphenols in terms of linearity, specificity, limits of detection and quantification, intra-day and inter-day p...

Background: Black garlic is obtained from raw garlic (Allium sativum L.), by a fermentation process, under humidity and heat treatment, showing a high concentration of organosulfur compounds, which have been related to benefits in the prevention or delay of cardiovascular diseases (CVDs). The objective of the research was to evaluate whether long-...

Dentro de sector pesquero andaluz los productos derivados de la pesca son altamente demandados y consumidos, siendo la mojama un producto de excelencia y con una gran importancia en la región de Andalucía. El inconveniente principal para la elaboración de este producto es que se debe partir de un atún de partida que se haya congelado a una temperat...

We surveyed wild boar (Sus scrofa) populations using 16S rRNA gene analysis of the gut microbiota in fresh faeces taken from 88 animals hunted in 16 hunting estates. The wild boar is a very convenient model system to explore how environmental factors including game management, food availability, disease prevalence, and behaviour may affect differen...

Phenolic catabolites excreted by fasting subjects with a functioning colon and ileostomists on a low (poly)phenol diet have been investigated. Urine was collected over a 12 h fasting period after adherence to a low (poly)phenol diet for 36 h. UHPLC-HR-MS quantified 77 phenolics. Some were present in the urine of both groups in similar trace amounts...

The aim of this study was to investigate the changes in the formation of biogenic amines and free fatty acids occurring during the manufacturing process of a traditional dry-cured product based on yellowfin tuna, Thunnus albacares (protected geographical indication—Mojama) and how the different processing stages could affect food safety aspects. Th...

Onion (Allium cepa L.) and its newly derived product "black onion" are characterised by the presence of compounds with potential bioactivity, particularly organosulfur compounds (OSCs). However, little is known about the metabolism, distribution, and excretion of these compounds as they pass through the gastrointestinal tract. This study monitored...

Dry-cured tuna is a traditional product manufactured in the Mediterranean region of Spain, known as mojama. The traditional salting-drying elaboration process attributes new organoleptic characteristics to the final product, changing its flavor, color, and nutritional value. This study aimed to evaluate the changes in physicochemical, biochemical,...

Aiming to improve the quality of cocoa, preconditioning of cocoa after harvesting and before fermentation has become an on-farm processing step of great interest in recent times. The present work aimed to evaluate the influence of a pre-drying process on the volatile composition of Ecuadorian bulk (Forastero and CCN-51) and fine-flavour (ETT103 and...

The consumption of black garlic has been related to a decreased risk of many human diseases due to the presence of phytochemicals such as organosulfur compounds (OSCs). However, information on the metabolization of these compounds in humans is limited. By means of ultra-high-performance liquid chromatography coupled with high-resolution mass spectr...

Brandy stabilization is an important step aimed at decanting the suspended organic and inorganic particles that may cause undesirable turbidity (cloudiness or haze) in brandies, affecting the physico-chemical stability, the organoleptic characteristics, and the consumer’s quality perception of the brandy. This phenomenon originates from insoluble s...

The aim of this study was to evaluate the impact of in vitro gastrointestinal digestion and colonic fermentation on the polyphenol compounds from different varieties of pistachio by UHPLC-HRMS analysis. The total polyphenol content decreased significantly, mostly during oral (recoveries of 27 to 50%) and gastric digestion (recoveries of 10 to 18%),...

Cocoa (Theobroma cacao L.) is one of the most widely consumed edible seeds in the world affected by on-farm processing. This study investigated the effect of different drying techniques, namely oven drying (OD), sun drying (SD), and a modification of sun drying using black plastic sheeting (SBPD), on the volatile profile of fine-flavor and bulk coc...

The impact of β-glucan-rich oat bran on the bioavailability of orange juice (OJ) flavanones was investigated. Volunteers consumed 500 mL of OJ with and without 22 g of oat bran containing 6 g of β-glucan (OB-6). Urine collected 12 h prior to and over a 0-24 h period post-supplementation was analysed by UHPLC-HRMS. Sixteen flavanone metabolites and...

In recent years, marine polyphenols including phlorotannins, bromophenols, and phenolic compounds have attracted a great deal of attention due to their potential health benefits against several non-communicative human diseases. A growing body of evidence indicates that the metabolites and colonic catabolites of parent polyphenols found in foodstuff...

The demand for organic food products has increased in recent years due to them being perceived healthier, safer, and eco-friendlier by consumers, boosting the development of this industry. The higher retailing price of organic products increases the risk of fraudulent practices, making it necessary to establish control mechanisms to authenticate th...

In this study, in vitro cultures and micropropagated plants of two Mediterranean aromatic plants, Lavandula viridis L’Hér and Thymus lotocephalus López and Morales, were exposed to different temperatures (15, 20, 25, and 30 °C). The effect of temperature on the levels of hydrogen peroxide (H2O2), lipid peroxidation, and osmoprotectants (proline, so...

Disruption of microvascular architecture is a common pathogenic mechanism in the progression of Alzheimer's disease (AD). Given the anti-angiogenic activity of berry (poly)phenols, we investigated whether long-term feeding of Rubus idaeus (raspberries) could ameliorate cerebral microvascular pathology and improve cognition in the APP/PS-1 mouse mod...

In nature, varieties of underutilized fruit-bearing plants are available and have remained underexploited for various reasons. However, the different parts of the plant, mainly the fruit, contributed considerably towards the sustainability of food as abundant sources of imperative phytochemical compounds and possess the potential for revenue genera...

To improve biological control and habitat management, how pest predators spread from natural habitats to crops must be understood. We studied whether intrinsic differences in stable‐isotopic ratios of C and N from an artificial C 4 (sugar‐cane) or C 3 (muti‐flower honey) diet could help mark and track predators that feed on them. Two aphid predator...

In mammals, the liver is involved in nutrient metabolism and in the regulation of lipid and glucose homeostasis. Multiple studies have described improvements in liver disorders after regular consumption of grape seed extract (GSE). GSE prevents or ameliorates hepatic metabolic dysfunction through AMPK activation, which reduces hepatic lipogenesis w...

The aim of this study was to investigate the changes in the formation of biogenic amines and free fatty acids occurring during the manufacturing process of a traditional salt-dried product based on yellowfin tuna, Thunnus albacares. (Protected Geographical Indication – Mojama ). Histamine levels found in all stages of the manufacturing process did...

Phenolic compounds are secondary plant metabolites known to be one of the most important sources of natural antioxidants in the human diet [...]

Stable isotopes have been widely used to obtain information on the ecology and nutritional quality of wildlife. The isotope relationships of stable carbon and nitrogen isotopes (δ13C and δ15N) present in different tissues are directly related to the diet of the animal and, to some extent, to the environment where the individual has developed. This...

The present research focused on studying the biogenic amine and volatile compound profiles of fresh yellowfin tuna (Thunnus albacares) and the resulted dry-cured product, the so-called “mojama”. This study aimed to evaluate how external factors such as the storage temperature in fishing vessels (EU regulation) and fishing season, may affect quality...

The impact of drought stress induced by polyethylene glycol (PEG) on morphological, physiological, (bio)chemical, and biological characteristics of Thymus lotocephalus López and Morales shoot cultures have been investigated, as well as the potential of iron oxide nanoparticles, salicylic acid, and methyl jasmonate (MeJA) as alleviating drought stre...

The results of the study of 11 pistachio varieties grown in Andalusia during the 2019 and 2020 campaigns are presented, for the content of total phenolic compounds, the antioxidant activity, the content of carotenoids, the content of chlorophyll. The color of the shell, the integument and the interior of the fruit is also studied. Licencia Creativ...

Pistachio (Pistacia vera L.) is a nut with a good adaptability to the Mediterranean conditions of cultivation, specifically in the Andalusian region, becoming an emerging crop. Moreover, it has been getting attention in the past years for the great content of bioactive compounds such as polyphenols. Although some studies have reported the polypheno...

The health properties related to onion intake are attributed mainly to the presence of bioactive compounds, particularly phenolic and organosulfur compounds (OSCs). The aim of this study was to investigate, for the first time, the effect of an in vitro colonic fermentation on the stability of phenolic and OSCs of fresh and black onion by ultra-high...

Avocado (Persea americana Mill) is a fruit consumed worldwide due to its valuable organoleptic and health-promoting properties. To date, no information is available about the isotopic composition of this foodstuff and this study determines the stable isotope composition of five bio-elements (C, N, S, H and O) and the composition profile of 46 macro...

Servifapa publica una revisión sobre la importancia de un buen control de la calidad y seguridad alimentaria en productos pesqueros y sus transformados gracias a la colaboración entre el Instituto Andaluz de Investigación y Formación Agraria, Pesquera, Agroalimentaria y de la Producción Ecológica (IFAPA) con el sector a través de proyectos de forma...

The excess of free radicals in the wound environment contributes to its stagnation during the inflammatory phase, favoring hard-to-heal wounds. Oxidative stress negatively affects cells and the extracellular matrix, hindering the healing process. In this study, we evaluated the antioxidant and wound-healing properties of a novel multifunctional amo...

A specific method for the simultaneous quantification of 28 esters in brandies using headspace solid-phase microextraction and gas-chromatography coupled to mass spectrometry (HS-SPME-GC–MS) was optimised and validated. First, the optimal extraction conditions were determined by using response surface methodology, which were obtained for an extract...

The analysis of stable isotopes in different tissues has been widely used to obtain information on the ecology and nutritional patterns of wildlife. The isotope ratios of the stable isotopes of carbon and nitrogen (δ¹³C and δ¹⁵N) analysed in different tissues are directly related to the animal's diet and, to some extent, to the environment where th...

The impact of β-glucan on the bioavailability of orange juice (OJ) flavanones was investigated in a randomised controlled trial. Volunteers consumed 500 mL of OJ without or with either 3 g (OB-3) or 6 g (OB-6) of β-glucan. Urine samples, collected 12 h before and over a 0–24 h period post-supplementation, were analysed by high-performance liquid ch...

Nanoparticles (NPs) recently emerged as new chemical elicitors acting as signaling agents affecting several processes in plant metabolism. The aim of this work was to investigate the impact of the addition of copper oxide (CuO), zinc oxide (ZnO) and iron oxide (Fe3O4) NPs (<100 nm) at different concentrations (1, 5 and 10 mg/L) to the culture media...

El análisis de isótopos estables ha sido ampliamente utilizado para la obtención de información sobre la ecología y calidad nutricional de la fauna silvestre. Las relaciones isotópicas de los isótopos estables de carbono y nitrógeno (d13C y d15N) presentes en diferentes tejidos están directamente relacionadas con la dieta del animal y, en cierto mo...

Entomopathogenic fungi are gaining acceptance in Integrated Pest Management (IPM) systems as effective and environmental safety biological control agents to protect a great variety of crops against pest insects. Many of these insect-pathogenic fungi can establish themselves as endophytes and thereby may induce the plant immune system. The activatio...

Revista Enólogos - Investigación y Ciencia, 132, 76-91 (2021). Brandy de Jerez is a spirit drink obtained from the ageing of wine distillates in the traditional and dynamic "Criaderas and Solera” sherry system. In this paper, the behavior of organic acids, volatile substances, esters, polyphenolic compounds, and metals in four "Criaderas and Solera...

Carob (Ceratonia siliqua L.) pod is a characteristic fruit from the Mediterranean regions. It is composed by seeds, the valuable part due to the extraction of locust bean gum, and the pulp, considered a by-product of the fruit processing industry. Carob pulp is a mixture of macro- and micronutrients, such as carbohydrates, vitamins and minerals, an...

This study evaluated the phenolic profile and the antioxidant capacity of strawberries (Fragaria x ananassa Duch., cv. Primoris) cultivated under reduction of nitrogen and adverse irrigation conditions (high salinity), such as those prevailing in Almeria (south-eastern Spain). The phenolic compound and anthocyanin profiles were analysed by HPLC-DAD...

A data-processing and statistical analysis workflow was proposed to evaluate the metabolic changes and its contribution to the sensory characteristics of different wines. This workflow was applied to rosé wines from different fermentation strategies. The metabolome was acquired by means of two high-throughput techniques: gas chromatography–mass spe...

Plant foods are consumed worldwide due to their immense energy density and nutritive value. Their consumption has been following an increasing trend due to several metabolic disorders linked to non-vegetarian diets. In addition to their nutritive value, plant foods contain several bioactive constituents that have been shown to possess health-promot...

The present study aimed at evaluating the effectiveness of different natural deep eutectic solvents (NADES) on the extraction of phenolic compounds from Lavandula pedunculata subsp. lusitanica (Chaytor) Franco, on the antioxidant activity, and acetylcholinesterase (AChE), butyrylcholinesterase (BChE) and tyrosinase (Tyr) inhibitory capacities. Ten...

The genus Citrus amounts to one of the most relevant fruit crops in the world. This work assays overall fruit quality information in order to ascertain the optimal harvest timing of three late-season orange cultivars (‘Barberina’, ‘Valencia Midknight’ and ‘Valencia Delta Seedless’) during two consecutive growing seasons. Thus, morphological and phy...

NIRS Near Infrared Spectroscopy a non-destructive analytical technique that allows to differentiate bitter almonds, with more than 5 mg / g of amygdalin, from non-bitter ones. This technique can be implemented in the production line, so that all the almonds are analyzed and those that are bitter can be separated from the rest. integration into the...

Black carrot has been attracting increasing thanks to its high bioactive compound content. This study presents the polyphenol bio-accessibility of black carrot and two derived products (black carrot snack (BC snack) and black carrot seasoning (BC seasoning)) after in vitro gastrointestinal digestion and colonic fermentation. Additionally, antioxida...