José Salvador Montaña

Pontificia Universidad Javeriana · Department of Microbiology

Microbial enrichments with a substrate of interest could enhance the possibility of finding certain desired metabolic activities. As lipases are one of the most important enzymes in industrial applications, the Colombian Andean soils were explored as a source of lipolytic microorganisms. Two Andean soils under low temperatures were sampled: paramo...

Microbial enrichments cultures are a useful strategy to speed up the search for enzymes that can be employed in industrial processes. Lipases have gained special attention because they show unique properties such as: broad substrate specificity, enantio- and regio-selectivity and stability in organic solvents. A major goal is to identify novel lipo...

A taxonomic and annotated functional description of microbial life was deduced from 53 Mb of metagenomic sequence retrieved from a planktonic fraction of the Neotropical high Andean (3,973 meters above sea level) acidic hot spring El Coquito (EC). A classification of unassembled metagenomic reads using different databases showed a high proportion o...

Phylogenetic affiliation of SSU rRNA sequences. The aligned reads were classified taxonomically using “classify a batch of sequences against multiple taxonomies tool” from Greengenes. (XLS)

Functional assignment of metagenomic sequences using BLASTX against NCBI-nr. The data was analyzed using MEGAN v4.0 software and KEGG identifiers. (PPT)

Taxonomic and functional clustering based on total reads of various metagenomes (RSM: Red Soudan Mine; BSM: Black Soudan Mine; POCR: Pacific Ocean (coral reefs); SW: Sea Water; PO: Pacific Ocean; TFS: Tropical Forest Soil; HAFS: High Andean Forest Soil; PMS: Pristine Mangrove Sediments; AHEC: Acidic Hot Spring EC). The data were compared to a) RefS...

Metagenomic reads obtained from EC hot spring that mapped to the A. cryptum JF-5, L. pneumophila str. Corby and A. caldus draft genomes. (XLS)

Partial a) nitrogen and b) sulfur pathways identified by KEGG affiliation of the sequences from EC hot spring. Boxes indicate the KEGG characteristic identified and numbers in gray circles indicate the amount of sequence reads affiliated to the KEGG function. (PPT)

In order to search new lipolytic enzymes and conduct bioprospecting of microbial communities from high Andean forest soil, a metagenomic library of approximately 20,000 clones was constructed in Escherichia coli using plasmid p-Bluescript II SK+. The library covered 80 Mb of the metagenomic DNA mainly from Proteobacteria, Actinobacteria and Acidoba...

p> Stevia rebaudiana is a plant that accumulates a non-caloric sweetener compound known as stevioside. This crop is traditionally fertilized with chemicals that are harmful for the ecosystem, forcing to find organic alternatives to mitigate this damage. Objective . To study the effect of organic matter and an Azotobacter nigricans -based bio-fert...

Stevia rebaudiana is a plant that accumulates a non-caloric sweetener compound known as stevioside. This crop is traditionally fertilized with chemicals that are harmful for the ecosystem, forcing to find organic alternatives to mitigate this damage. Objective. To study the effect of organic matter and an Azotobacter nigricans-based bio-fertilizer...

Total metagenomic DNA was isolated from high Andean forest soil and subjected to taxonomical and functional composition analyses by means of clone library generation and sequencing. The obtained yield of 1.7 μg of DNA/g of soil was used to construct a metagenomic library of approximately 20,000 clones (in the plasmid p-Bluescript II SK+) with an av...

With the purpose of isolating and characterizing free nitrogen fixing bacteria (FNFB) of the genus Azotobacter, soil samples were collected randomly from different vegetable organic cultures with neutral pH in different zones of Boyacá-Colombia. Isolations were done in selective free nitrogen Ashby-Sucrose agar obtaining a recovery of 40%. Twenty f...

Bio-fertilizer production from an isolate of Azotobacter nigricans obtained from a plantation of Stevia rebaudiana Bert. Objective.To isolate nitrogen fixing bacteria to be used in a fertilization regime of an organic agriculture program. Materials and methods. Theisolation of nitrogen fixing bacteria was done in an Ashby-benzoate medium from soil...

Objective. To isolate nitrogen fixing bacteria to be used in a fertilization regime of an organic agriculture program. Materials and methods. The isolation of nitrogen fixing bacteria was done in an Ashby-benzoate medium from soil of a Stevia rebaudiana plantation. Isolates identified as Azotobacter nigricans were evaluated by their growth kinetics...

o C y períodos de luz constante y fotoperíodo 24 h luz/24 h oscuridad durante 8 días. Los parámetros estimados fueron densidad poblacional (conidios/mL), germinación de esporas a 24 horas y porcentaje de pureza. Los resultados indicaron q ue el proceso de fermentación sólida empleando como sustrato arroz-agua destilada a 25ºC y la exposición consta...

In order to evaluate the effect of temperature and light conditions on biomass production of a Trichoderma sp. strain, three culture media were tested: rice 53% (w/w), rice 53% (w/w) -molasses 3% (w/w) and rice 53% (w/w)-molasses 10% (w/w) in distilled water. Incubation conditions were: 25°C, constant light and a photoperiod of 24 h light/24 h dark...

A Lambda FIX II genomic library consisting of 1,33x10 6 pfu/ml with an average DNA insert size of 15 kb was constructed from Coffea arabica var Colombia DNA. The haploid coffee genome was represented 3,7 times approximately. The library was evaluated with a coffee PCR product (primers InhF - R631) which has a high homology with Arabidopsis thaliana...