José Luiz Pedreira Mouriño

• Professor
• Head of Department at Universidade Federal de Santa Catarina

This study evaluated how the levels of methyl chavicol and linalool behave in tissues of juvenile Nile tilapia after anesthesia with the essential oil (EO) of Ocimum basilicum at different temperatures. The EO was diluted in ethanol (1:9), and the fish (64.99 ± 13.90 g) were immersed in the anesthetic solution (250 mg L⁻¹) at 23 and 27 ºC for 10 mi...

The present field study evaluated the effects of a commercial multi-strain bioremediator (Thiobacillus denitrificans, Bacillus subtilis, Bacillus licheniformis, and Paracoccus sp.) on the frying phase of Nile tilapia (Oreochromis niloticus) under biofloc conditions. The bioremediator was applied directly to the biofloc water at a dosage of 3 g m3 (...

Raw materials of plant origin, such as rice bran (Oryza sativa; RB), are promising alternatives to fishmeal in aquaculture feeds, offering a low-cost solution. However, due to antinutritional factors and reduced digestibility, direct use of RB is limited. Fermentation is an effective, cost-effective, and environmentally friendly technique that impr...

The aquafeed industry evolved alongside fish farming, utilizing scientific and technological advancements to incorporate a variety of feed additives, supplements, and alternative ingredients in the nutrition and feeding of fish in aquaculture. These advances played a significant role in improving the production, health, and welfare of farmed fish....

Currently, nanotechnology stands out as a wave of innovation across various fields, including aquaculture. Given the challenges related to fish health and the limitations of conventional control methods, more effective and sustainable alternatives are increasingly needed. On this context, metallic nanoparticles emerge as a promising solution due to...

The study aimed to evaluate the in vitro anthelminthic and antimicrobial activity of silver nanoparticles (AgNPs) against Dactylogyrus minutus and Aeromonas hydrophila, pathogens of Cyprinus carpio Koi. Gill arches of the fish were removed and placed into six-well plates containing 10 mL of tank water with varying concentrations of AgNPs: 100, 400,...

We aimed to identify the digestive microbiota of the golden mussel Limnoperna fortunei, and characterize its structure in different seasons. Limnoperna fortunei specimens were sampled in winter (September 2019) and summer (March 2020) in five reservoirs of hydroelectric power plants in southern Brazil. In each reservoir, we sampled 15 individuals a...

This study evaluated the supplementation of iron and copper nanoparticles in channel catfish diets and their influences on growth and health. A comparative feeding trial was carried out for 9 weeks to evaluate combinations of iron and copper nanoparticles: only iron nanoparticles (IronNP), only copper nanoparticles (CopperNP), CopperNP + IronNP, an...

This study evaluated the effects of interactions between β-glucan-enriched complex and vitamin premix on the zootechnical performance and histological and histomorphological parameters of the gastrointestinal tract of juvenile Nile tilapia (Oreochromis niloticus). The diets were supplemented with a manufacturer-indicated dosage of vitamin Premix at...

Nile tilapia Oreochromis niloticus is the most cultivated fish species in continental waters and represents the second most important group for global aquaculture. In Brazilian continental fish farming, diseases represent an annual loss of approximately US$84 million. Aiming to minimize these adversities, researchers are constantly investigating vi...

The aim of this study was to evaluate the effect of supplementing feed with a functional mixture of immunomodulators, including β-glucans, nucleotides, ascorbic acid, and alpha-tocopherol, associated with a diet with lower levels of animal protein (11.5%) and higher levels of soybean meal (43.5%), on the zootechnical performance, health, hematologi...

The study evaluated dietary supplementation with a feed additive composed of multi-strain Bacillus for Nile tilapia Oreochromis niloticus. In vitro and in vivo assays employing culture-based microbiological methods and metagenomics were performed. Additionally, the study assessed the haemato-immunology, intestinal microbiome, and growth performance...

Despite all the technical-scientific knowledge about streptococcosis, the high prevalence of bacterial infections caused by Streptococcus spp. in Nile tilapia farming implies the frequent, often irresponsible, use of antibiotics. The use of chemotherapy in aquaculture environments remains an efficient practice in the treatment of bacterial infectio...

The study evaluated the effects of dietary supplementation with Lippia sidoides essential oil on the microbiota and intestinal morphology of Danio rerio. For this, 448 fish were randomly distributed in 28 tanks divided into a control group fed a commercial diet without supplementation, a group fed a commercial diet containing grain alcohol and five...

The study evaluated the effects of the probiotic BioPlus® PS on shrimp-tilapia polyculture pond soil using an integrated approach of physical-chemical analysis, metagenomics, and Pfeiffer circular chromatography. Soil was collected from a shrimp farm immedi-ately after harvest and distributed in 75 plastic boxes with a useful volume of 1 L. The pro...

In this study, we analyzed the hematoimmunological effects of dietary supplementation with immunomodulators (β-glucans + nucleotides) and different levels of vitamins on Nile tilapia (Oreochromis niloticus) after exposure to physical stress. The following four diet treatments were used: diets with indicated vitamin levels (Vitind), diets with Vitin...

This study aimed to evaluate the potential of Lippia sidoides essential oil as a food additive for Cyprinus carpio koi carp in improving the health parameters and resistance to infection by Aeromonas hydrophila. A total of 312 carp were divided into groups. Twenty-four animals were in the lethal dose 50% (LD50) test group. The remaining 288 were di...

The nutritional quality of food can affect the health of animals. This study examined the effects of dietary supplementation with Lippia sidoides essential oil (LSEO) on the physiology of Danio rerio. Four hundred fourty-eight fish were divided into 28 tanks and subjected to different dietary treatments: a control group with no supplementation, a g...

Feed additives, such as β-glucans, nucleotides, ascorbic acid (vitamin C), and α-tocopherol (vitamin E) can improve fish immunity and contribute to enhanced zootechnical performance of the fish. This study aimed to evaluate the effect of different concentrations of vitamin- mineral premix with or without the inclusion of an immunostimulant boost (β...

Caros leitores e entusiastas da piscicultura brasileira, é com grande satisfação que apresentamos o livro "Enfermidades parasitárias e bacterianas na piscicultura brasileira: insights e perspectivas". Este livro tem como objetivo reunir informações relevantes sobre as principais enfermidades bacterianas e parasitárias que afetam os peixes nos siste...

A ideia deste livro sempre surgia no início de cada semestre da disciplina "Ranicultura", que ministrei na FCAV (Faculdade de Ciências Agrárias e Veterinárias) - UNESP. Ao apresentar aos alunos de Zootecnia o programa do curso e as bibliografias recomendadas, não havia, até então, um material que centralizasse todo o conteúdo. O sonho se concretizo...

Simple Summary Monogeneans are problematic parasites in fish production because of their epizootic effects and difficulty in administering control/treatment in the production environment. Understanding how abiotic and biotic factors influence the dynamics of these parasites is the first step towards developing efficient strategies for the managemen...

This study evaluated the effects of microalgae (Scenedesmus obliquus) addition and fish feed supplementation on hemato-immunological parameters of Pacific white shrimp (Penaeus vannamei) and juvenile Nile tilapia (Oreochromis niloticus) cultured in integration using biofloc technology for 62 days. The experiment was designed as a factorial design w...

This study evaluated the effect of Lippia sidoides essential oil (LSEO), at a concentration of 0.25%, on the inflammatory response and histological parameters in Danio rerio zebrafish. Three hundred and fifteen fish were equally distributed into 21 aquaria and divided into three groups with three replicates (negative control (NC), diet containing g...

The objective of this study is to contribute to the sustainable growth of aquaculture through the evaluation of zinc and copper nanoparticles as health promoters and preventives to infections. In TEST 1 the validation of products validation of products based on zinc nanoparticles (Nano-ZnO), chelated or not with methionine (LM). In the TEST 2 will...

The aim of this study was to evaluate the influence of 0%, 0.1%, 0.2%, or 0.3% organic benzoic acid on the intestinal microbiota of Nile tilapia (Oreochromis niloticus). Classical medium-dependent microbiology, histology, and metagenomics analyses used high-throughput sequencing. Fish (n = 320) were divided into 16 tanks, with four fish per group:...

Caros leitores e entusiastas da piscicultura brasileira, é com grande satisfação que apresentamos o livro "Enfermidades parasitárias e bacterianas na piscicultura brasileira: insights e perspectivas". Este livro tem como objetivo reunir informações relevantes sobre as principais enfermidades bacterianas e parasitárias que afetam os peixes nos siste...

The aim of the study was to evaluate the brain tissue and blood of Nile tilapia after ingestion of feed contaminated by Streptococcus agalactiae. 90 fish were allocated in six experimental units, four treatments and two control units, totalling 15 animals per tank. The fish received the bacterial inoculum via infected feed, with a volume of 100 μL...

The increment of decomposition of organic matter in sediment samples from Nile tilapia farms was evaluated with the introduction of Bacillus subtilis and B. licheniformis bacteria. Methods: Sediment samples placed in 18L plastic boxes received single dose inoculum with the following concentrations: 1.21 x 106 CFU g-1 (equivalent to 75 g ha-1), 2.41...

This is a short communications article in which we performed the predictive functional profile of the water bacterial community in the integrated culture of Pacific white shrimp and Nile tilapia under mature and heterotrophic biofloc systems, using the FAPROTAX database. In brief, we found that it was possible to differentiate the samples from the...

This study evaluated the effects of autochthonous single‐strain and multi‐strain (mix) probiotics on the zootechnical performance and sanitary conditions of juvenile neotropical fish. Fingerlings of tambaqui (Colossoma macropomum) were fed three diets of single‐strain probiotics (two Enterococcus faecium strains and one Bacillus cereus autochthonou...

This study evaluated the mortality rate, histopathology and haematological parameters of Arapaima gigas against Aeromonas hydrophila after 68 days of dietary probiotic supplementation with autochthonous bacterium Enterococcus faecium. Three experiments were carried out: the first assay evaluated the lethality of A. hydrophila, the second and the th...

This study aimed to isolate a yeast strain and evaluate its potential to be inoculated in the biofloc technology culture of Pacific white shrimp employing in vitro and in vivo assays. A yeast strain identified as Meyerozyma guilliermondii (CPQBA 1459/17 DRM-01) was isolated from the soil of an estuary. Its growth kinetics under different salinities...

The study was carried out to evaluate the effect of dietary supplementation of three probiotic strains in isolation and a multi-strain formulation (mix) on the rearing of tilapia, based on the parameters of productive performance and resistance to infection. A total of 240 juveniles of Oreochromis niloticus (6.71 ± 0.93 g and 61.88 ± 1.44 mm) were...

O estudo avaliou o desempenho produtivo de duas linhagens de Oreochromis niloticus, GIFT (Genetically Improved Farmed Tilapia) e Chitralada (Thai), cultivadas em viveiros escavados com temperaturas sub-ótimas. Os viveiros foram povoados com densidade de dois peixes m-3 e três repetições para cada grupo. Inicialmente, os peixes foram alimentados com...

The study aimed to evaluate the oral infected-feed, intragastric-gavage, and intraperitoneal routes of the Streptococcus agalactiae infection in Nile tilapia (Oreochromis niloticus). For this purpose, 270 juveniles of Nile tilapia, with an average weight of 2 g, were distributed in 18 experimental units of 90 L, acclimatized, and raised for 55 days...

This study aimed to verify the effects of dietary supplementation with sodium butyrate and Lippia origanoides, combined and isolated, on the health and zootechnical performance of Nile tilapia juveniles Oreochromis niloticus. A total of 120 fish (5.38 ± 0.65 g) were randomly distributed in 12 experimental units and fed different experimental diets...

This study aimed to verify the effects of dietary supplementation with sodium butyrate and Lippia origanoides, combined and isolated, on the health and zootechnical performance of Nile tilapia juveniles Oreochromis niloticus. A total of 120 fish (5.38 ± 0.65 g) were randomly distributed in 12 experimental units and fed different experimental diets...

The aim of this study was to evaluate the effects of dietary supplementation with benzoic organic acid in different concentrations on juvenile Nile tilapia Oreochromis niloticus. Was analyzed the growth performance, hemato-immunological parameters and survival after challenge via gavage with Streptococcus agalactiae. A total of 320 juvenile fish we...

We evaluated the growth, gut histomorphometry, selected immune responses and resistance of Nile tilapia (Oreochromis niloticus) against Streptococcus agalactiae infection when fed VILIGEN™, an additive blend containing sodium butyrate, dehydrated hydrolysed yeast and zinc proteinate. Increasing doses (0.00, 0.60, 1.20, 2.40 and 4.80 g kg⁻¹) were ad...

The aim of this work was to isolate, identify and characterize microorganisms present in intensive nursery of Penaeus vannamei during the spontaneous generation of the biofloc and to verify among the isolates, beneficial strains for the intensive cultivation of shrimp under biofloc conditions. 91 strains were isolated and identified by molecular ta...

The aim of this study was to investigate the effect on fish health indicators of different Nile tilapia (Oreochromis niloticus) stocking densities when reared in an integrated culture with Pacific white shrimp (Litopenaeus vanna-mei) using biofloc technology for 57 days. Three fish densities were evaluated: 8, 16 and 24 fish tank − 1 with four repl...

The aim of the present study was to investigate the influence of dietary supplementation with Bacillus subtilis C-3102 on the productive, intestinal histomorphometry, haemato-immunological aspects, as well as evaluated the resistance of juvenile Pseudoplatystoma sp. after challenge with Aeromonas hydrophila. Inclusion levels of the commercial probi...

Thirty groups of twenty juveniles each of Nile tilapia (Oreochromis niloticus, initial average weight 23.93 ± 0.80 g) were fed diets containing increasing amounts (0%, 25%, 50%, 75%, and 100% of levels used by the tilapia feed industry) of organic trace mineral (Fe, Zn, Mn, Cu, Se) supplementation for 56 days. Growth was not affected by the dose of...

The intensification of aquaculture production systems in recent years has been accompanied by increased parasitic infections resulting in significant losses in fish aquaculture requiring chemicals to control diseases. The conventional treatments are known to cause negative side effects, both for the environment and for human and animal health. Laws...

Litopenaeus vannamei or Pacific white shrimp is one of the main shrimp species cultivated around the world. Despite its easy handling, there is no detailed description of its metabolic performance. This work developed a novel mathematical model for L. vannamei growth based on mass conservation and chemical equations for feed oxidation, allowing to...

The present study reviewed the literature published in recent years on the topic “Recirculating Aquaculture Systems (RAS)”, a technology that makes it possible to grow aquatic organisms more sustainably and environmentally friendly, reducing water consumption, environmental pollution and land use. Commercial and academic application of recirculatin...

This study evaluated the effect of exogenous digestive enzymes on the survival, growth performance and morphology of the digestive tract of juveniles of the longsnout seahorse Hippocampus reidi fed exclusively with Artemia and supplemented with five different concentrations of porcine pancreatin (PP) (0, 5, 25, 50 and 75 mg/L from birth until 30 da...

The aim of the present study was to propose a low-cost nitrogen removal system through the nitrification / denitrification process in order to maintain the water quality required for the Pacific white shrimp superintensive cultivation in closed systems without water renewal. The increase in productivity consequently causes the accumulation of organ...

Brazilian sardine is emerging as a promising species in Aquaculture. This study describes for the first time a case of parasitic infestation by Amyloodinium in Brazilian sardines Sardinella brasiliensis obtained from natural spawning in captivity. The sardines kept in nurseries were naturally parasitized by the amylodiniosis causative agente the dy...

Abstract Brazil is following the growing trend of world aquaculture production. Among the cultured fish species, Tilapia (Oreochromis niloticus) presents itself as a potential commodity in the “aquabusiness” sector owing to its versatility. This study was conducted to evaluate the economic-financial viability of tilapiculture in southern Brazil. Th...

The aims of this study were to evaluate the inclusion of different concentrations of Lactobacillus plantarum in Nile tilapia diet and to verify histological effects on tissues of the animal organs, as well as to verify its effects on growth parameters and possible increase in the immune system. A total of 240 juveniles were distributed in 16 tanks...

Aim The increment of decomposition of organic matter in sediment samples from Nile tilapia farms was evaluated with the introduction of Bacillus subtilis and B. licheniformis bacteria. Methods Sediment samples placed in 18L plastic boxes received single dose inoculum with the following concentrations: 1.21 x 10⁶ CFU g⁻¹ (equivalent to 75 g ha⁻¹),...

Abstract The aim of this study was to characterise possible histopathological and haemato-immunological changes after subclinical infection by S. agalactiae S13 serotype Ib. One hundred juveniles of Nile tilapia with average weight of 45 g were distributed in ten 90 L experimental units. After the acclimation period 25 fish were euthanised, and fra...

This study evaluated the in vitro antiparasitic activity of the essential oils of Lippia alba, L. origanoides and L. sidoides against monogenean parasites of koi carp Cyprinus carpio and its zootechnical performance in net cages. The oils were obtained from the leaves by hydro distillation, and the chemical composition was evaluated via gas chromat...

Intensive fish farming has resulted in an increased concern for disease outbreaks. Probiotic use is one of the strategies being developed to improve fish health and productivity. Measures of probiotic colonization, growth performance, haemato‐ logical characteristics and parasite load were used to evaluate the effect of diets supplemented with Ente...

The aim of this study was to obtain an autochthonous probiotic candidate strain from the silver catfish (Rhamdia quelen) intestinal tract, comparing its in vivo performance with an allochthonous probiotic isolated from another fish, Nile tilapia (Oreochromis niloticus), in a growth performance assay. The study was divided in two parts: in vitro and...

This study evaluated the effects of dietary supplementation of organic acids blend (OAB) alone or in combination with essential oil, Lippia origanoides (OAE) for Nile tilapia fed supplemented diets for 30 days. Fish (1.1 ± 0.04 g) were fed control (Control), or OAB 0.5% or OAB 0.5% + essential oil 0.125% (OAE) respectively. At the end of the experi...

This study aimed to investigate the ability of two probiotic strains (Lactococcus lactis subsp. lactis and Enterococcus faecium) to colonize Arapaima gigas intestines and their competence in modulating gut microbiota, morphology, and immunological status. A total of 135 A. gigas (58.86 ± 10.25 g) were divided into three treatments: (a) fish fed a d...

The production of ornamental fishes has been intensified to attend the global market. With this expansion, an increase in infections and infirmities has been observed, most of which are attributed to pathogenic bacteria. Dietary supplements to improve growth and immunity of these animals have been introduced as a safe way to control and prevent dis...

This study evaluated the use of biopromoter Gamaxine for fish, a product elaborated from the fermentation of purified cultures of inactivated saprophytic bacteria, at the original concentration of 1.0 g L⁻¹, to favor the equilibrium of the intestinal microbiota, providing better protection against the enteric challenges. It is used originally as ag...

774/5000 Coordinated by Laboratório de biologia e Cultivo de Peixes de Água Doce (LAPAD). The main objective is to understand the presence of the golden mussel and seek mechanisms for biological control of the mollusk in the waters of the reservoirs of the hydroelectric plants of the Canoas, Pelotas and Uruguay rivers, located in the west of Santa...

The aim of the present study was to verify the effect of sodium butyrate in protected and unprotected forms at two different concentrations of diet inclusion (0.25% or 0.5%) on the digestive enzyme's activity, integrity and structure of the intestinal tract and liver, in juveniles of Oreochromis niloticus, during the period of sexual reversion. In...

Food additives based on organic acids or their salts promote numerous improvements in zootechnical parameters, digestive enzymatic activities, resistance to diseases and intestinal health. This study aimed to evaluate the influence of calcium and sodium chelated to propionic acid on the digestive enzymes activities, intestinal microbial community a...

Perkinsus spp. have been detected in various bivalve species from north‐east Brazil. Santa Catarina is a South Brasil state with the highest national oyster production. Considering the pathogenicity of some Perkinsus spp., a study was carried out to survey perkinsosis in two oyster species cultured in this State, the mangrove oyster Crassostrea gas...

Fish may bioaccumulate contaminants from the aquatic environment and extend them to the food chain provoking risks to human health. This study evaluated the microbiological parameters of the ponds water and trace elements concentrations in samples of water, sediment, feed and muscle of farmed Nile tilapia used for human consumption in southern Braz...

This study investigated the use of silymarin, an extract obtained from the milk thistle (Silybum marianum) and its effects as a possible hepatoprotector in Nile tilapia (Oreochromis niloticus). Silymarin was used as feed additive to the diet at a concentration of 0.1% (1 kg per ton of dry ration) with the commercial product named Di-Heptarine S® (1...

This study evaluated the effects of dietary inclusion of sardine (Sardinella spp.) protein hydrolysate (SPH) on productive performance, digestive enzymatic activity, morphology and intestinal microbiology of South American catfish (Rhamdia quelen) juveniles. SPH was produced from sardine processing wastes using Protamex® protease complex from Novoz...

The present study evaluated the effect of pure and protected sodium butyrate with palm oil and buffered solution supplemented in the diet of Nile tilapia Oreochromis niloticus on the performance parameters, hematological parameters and disease resistance during sexual reversion. Initially, the minimum inhibitory concentration (MIC) of sodium butyra...

Com grande potencial para a aquicultura, o robalo-peva necessita de tecnologias para a produção massiva de juvenis com alta qualidade para pré-engorda. Uma forma de se obter melhores resultados com peixes saudáveis e resistentes seria a utilização de probióticos. Foi verificada a influência da suplementação de Lactobacillus plantarum à dieta de juv...

Intensive aquaculture production requires new strategies to improve the performance and health parameters of farmed fish to avoid developing resistance in bacterial genes and to avoid environmental pollution. The aim of this study was to evaluate the effects of calcium and sodium as chelants to propionic acid on the growth performance and hemato-im...

The aim of this study was to evaluate the effect of dietary supplementation with the autochthonous probiotic bacteria Bacillus cereus on growth performance, haematological parameters and survival of tambaqui (Colossoma macropomum). Tambaqui fingerlings (0.94 ± 0.02 g) were fed for 120 days the following diets: basal (without probiotic) and three su...

O presente estudo avaliou a suplementação dietária com óleo essencial de Lippia alba sobre os parâmetros hemato-imunológicos em tilápias do Nilo (Oreochromis niloticus) submetidas à inflamação aguda induzida por carragenina na bexiga natatória. Pelo período de 45 dias, 96 peixes foram divididos em quarto tratamentos em triplicata: a) peixes supleme...

In order to reduce disease outbreaks in shrimp aquaculture, biosafe farming practices have been created, such as the biofloc system and the use of feed additives to improve shrimp immune response and growth performance. Many studies with feed additives have tested probiotics in biofloc rearing systems; yet to date, no studies have reported on the u...

Dietary supplementation with natural products is a promising alternative for increasing immunological capacity and disease resistance in farmed fish. However, information about the effects of these additives on morphology and functionality of fish organs are scarce. The aim of this study was to verify the effects of dietary supplementation with ess...

Nile tilapia (Oreochromis niloticus) is the most farmed freshwater fish in the world, however, disease outbreaks are the main cause of losses in production. Due to this, there is an increasing interest in natural products for enhancing disease resistance, without causing physiological impairment. The aim of this study was to verify the effect of th...

RESUMO O objetivo deste estudo foi isolar cepas de bactérias ácido-lácticas do trato intestinal de robalos-peva e avaliar o seu potencial probiótico. Foram isoladas três cepas bacterianas, das quais duas inibiram o crescimento in vitro de Vibrio harveyi e V. alginolyticus. Destas cepas, a que apresentou maior crescimento in vitro (Lactococcus sp.)...

The aim of this study is to select and isolate autochthonous bacteria with probiotic potential for use in a supplemented diet for bullfrog tadpoles, Lithobates catesbeianus. A total of 20 strains of lactic acid bacteria were isolated. Nine out of these were used in the following in vitro assays: antagonism against pathogenic bacteria (ANT), antimic...

A aquicultura encontra-se em franca expansão no mundo e no Brasil. Os peixes criados em sistemas aquícolas representaram 42 % do total de 158 milhões de toneladas obtidas no somatório da pesca de captura e da aquicultura no ano de 2012. Estima-se que a produção continental brasileira de peixes dulcícolas esteja próxima de 600 mil toneladas sendo Sa...

ABSTRACT This study aimed to assess the final body weight, weekly weight gain, yield, apparent feed efficiency, protein efficiency ratio, and feed intake of the Pacific white shrimp (Litopenaeus vannamei) fed four diets containing different levels of soy protein concentrate (SPC) as a replacement for fishmeal, reared in a super-intensive biofloc sy...