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Jose Luis Toca-Herrera

Jose Luis Toca-Herrera
University of Natural Resources and Life Sciences Vienna (BOKU) · Nanobiotechnology (Biophysics)

PhD in Physical Chemistry, MSc in Physics

About

183
Publications
40,062
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Introduction
At the Institute of Biophysics at BOKU we are working the following topics: 1. Mechanical properties of biological materials (proteins, hydrogels, cells). 2. Molecular and colloidal forces. 3. Self assebly processes related to interface formation (membrane and crystal formation). 4. Micro and nano characterization of biological materials. 4. Applied physical models in other scientific fields (computers, sport, tourism).
Additional affiliations
September 2010 - present
University of Natural Resources and Life Science Vienna (BOKU)
Position
  • Full professor (chair holder)
Description
  • Former teaching at University Rovira i Virgili and University of the Basque Country (2004-2009). Visiting professor at Polytechnic University of Valencia (2009) and AGH-Krakow (2015).

Publications

Publications (183)
Article
Cells are complex, viscoelastic bodies. Their mechanical properties are defined by the arrangement of semiflexible cytoskeletal fibers, their crosslinking, and the active remodeling of the cytoskeletal network. Atomic force microscopy (AFM) is an often‐used technique for the study of cell mechanics, enabling time‐ and frequency‐dependent measuremen...
Article
Biopolymers, such as polynucleotides, polypeptides and polysaccharides, are macromolecules that direct most of the functions in living beings. Studying the mechanical unfolding of biopolymers provides important information about their molecular elasticity and mechanical stability, as well as their energy landscape, which is especially important in...
Article
A‐to‐I RNA editing by ADARs is an abundant epitranscriptomic RNA‐modification in metazoa. In mammals, Flna pre‐mRNA harbors a single conserved A‐to‐I RNA editing site that introduces a Q‐to‐R amino acid change in Ig repeat 22 of the encoded protein. Previously, we showed that FLNA editing regulates smooth muscle contraction in the cardiovascular sy...
Chapter
Using physical models to describe the response of cells to external stimuli has grown popular in the last three decades. The mechanical properties of cells are tightly linked to biochemical signaling pathways related to cell structure, proliferation, differentiation, motility, and cell fate. This chapter aims to describe how to perform mechanical e...
Article
Cells sense stiffness of surrounding tissues and adapt their activity, proliferation, motility and mechanical properties based on such interactions. Cells probe the stiffness of the substrate by anchoring and pulling to their surroundings, transmitting force to the extracellular matrix and other cells, and respond to the resistance they sense, main...
Article
Full-text available
Excessive estrogen exposure is connected with increased risk of breast cancer and has been shown to promote epithelial-mesenchymal-transition. Malignant cancer cells accumulate changes in cell mechanical and biochemical properties, often leading to cell softening. In this work we have employed atomic force microscopy to probe the influence of estro...
Article
Atomic force microscopy (AFM) is the most often used tool to study the mechanical properties of eukaryotic cells. Due to their complex assembly, cells show viscoelastic properties. When performing experiments, one has to consider the influence of both loading rate and maximum load on the measured mechanical properties. Here, we employed colloidal p...
Article
Opiorphin (Oph) is a naturally produced endogenous peptide with a strong analgesic effect, superior to that of morphine, and without the severe side effects that morphine and morphine-like drugs exert. However, despite its strong therapeutic potential, the short duration of action, probably due to its low chemical stability and rapid degradation by...
Article
Full-text available
Beta-2-glycoprotein I (β2GPI) is a blood protein and the major antigen in the autoimmune disorder antiphospholipid syndrome (APS). β2GPI exists mainly in closed or open conformations and comprises of 11 disulfides distributed across five domains. The terminal Cys288/Cys326 disulfide bond at domain V has been associated with different cysteine redox...
Article
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Endothelial cells and astrocytes preferentially express metabotropic P2Y nucleotide receptors, which are involved in the maintenance of vascular and neural function. Among these, P2Y1 and P2Y2 receptors appear as main actors, since their stimulation induces intracellular calcium mobilization and activates signaling cascades linked to cytoskeletal r...
Article
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Monitoring biomechanics of cells or tissue biopsies employing atomic force microscopy (AFM) offers great potential to identify diagnostic biomarkers for diseases, such as colorectal cancer (CRC). Data on the mechanical properties of CRC cells, however, are still scarce. There is strong evidence that the individual zinc status is related to CRC risk...
Article
Full-text available
Cytoskeleton-disrupting drugs can have different effects on cell mechanics at different deformation scales. We therefore applied two complementary indentation techniques to study the effects of two actin-disrupting drugs on cellular stiffness of human umbilical vein endothelial cells. Optical tweezers were used to probe the cortical stiffness at sm...
Article
Within the field of neural tissue engineering, there is a huge need for the development of materials that promote the adhesion, aligned migration and differentiation of stem cells into neuronal and supportive glial cells. In this study, we have fabricated bioresorbable elastomeric scaffolds combining an ordered nanopatterned topography together wit...
Article
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An assessment tool to evaluate the degradation of biodegradable materials in a more physiological environment is still needed. Macrophages are critical players in host response, remodeling and degradation. In this study, a cell culture model using monocyte-derived primary macrophages was established to study the degradation, macro-/micro-mechanical...
Article
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This Special Issue is focused on measuring and characterizing the mechanical and adhesive properties of cells and membranes [...]
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Binary toxin (Bin) is one of the bio-larvicidal toxin produced by Lysinibacillus sphaericus. Two component proteins, BinA and BinB toxins are required simultaneously to exert its larvicidal activity. The binary toxin has been proposed to act initially on the susceptible cell membrane. Here, the cell membrane binding of the binary toxin was imitated...
Article
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The replacement of the cantilever tip by a living cell in Atomic Force Microscopy (AFM) experiments permits the direct quantification of cell–substrate and cell–cell adhesion forces. This single-cell probe force measurement technique, when complemented by microscopy, allows controlled manipulation of the cell with defined location at the area of in...
Article
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Cytolytic toxin (Cyt) is a toxin among Bacillus thuringiensis insecticidal proteins. Cyt toxin directly interacts with membrane lipids for cytolytic action. However, low hemolytic activity is desired to avoid non-specific effects in mammals. In this work, the interaction between Cyt2Aa2 toxin and model lipid bilayers mimicking the erythrocyte membr...
Article
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The aim of this study was to analyze the binding interactions between a common antihypertensive drug (ramipril, R) and the widely distributed plant flavonoid quercetin (Q), in the presence of human serum albumin (HSA). From the observed fluorescence spectra of the (HSA + R) system we can assume that ramipril is also one of the Site 3 ligands—simila...
Article
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A novel approach to local functionalization of plasmonic hotspots at gold nanoparticles with biofunctional moieties is reported. It relies on photo-crosslinking and attachment of a responsive hydrogel binding matrix by the use of UV interference field. A thermo-responsive poly(N-isopropylacrylamide) – based (pNIPAAm) hydrogel with photocrosslinkabl...
Article
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The interest in studying the mechanical and adhesive properties of cells has increased in recent years. The cytoskeleton is known to play a key role in cell mechanics. However, the role of the microtubules in shaping cell mechanics is not yet well understood. We have employed Atomic Force Microscopy (AFM) together with confocal fluorescence microsc...
Article
Full-text available
Correction for ‘Novel biodegradable and non-fouling systems for controlled-release based on poly(ε-caprolactone)/Quercetin blends and biomimetic bacterial S-layer coatings’ by Eva Sanchez-Rexach et al. , RSC Adv. , 2019, 9 , 24154–24163.
Article
Full-text available
Quercetin is a strong antioxidant with low bioavailability due to its high crystallinity. A further drawback is that Quercetin has potentially toxic effects at high concentrations. To improve this low water solubility, as well as control the concentration of the flavonoid in the body, Quercetin is incorporated into a polymeric matrix to form an amo...
Article
Atomic force microscopy (AFM) combined with fluorescence microscopy has been used to quantify cytomechanical modifications induced by resveratrol (at a fixed concentration of 50 µM) in a breast cancer cell line (MCF-7) upon temporal variation. Cell indentation methodology has been utilized to determine simultaneous variations of Young's modulus, th...
Article
Full-text available
Atomic force microscopy (AFM) is today an established tool in imaging and determination of mechanical properties of biomaterials. Due to their complex organization, those materials show intricate properties such as viscoelasticity. Therefore, one has to consider that the loading rate at which the sample is probed will lead to different mechanical r...
Article
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Collective (lattice) localized surface plasmons (cLSP) with actively tunable and extremely narrow spectral characteristics are reported. They are supported by periodic arrays of gold nanoparticles attached to a stimuli‐responsive hydrogel membrane, which can on demand swell and collapse to reversibly modulate arrays period and surrounding refractiv...
Article
Our purpose is to adapt a statistical method for the analysis of discrete numerical series to the keywords appearing in scientific articles of a given area. As an example, we apply our methodological approach to the study of the keywords in the Library and Information Sciences (LIS) area. Our objective is to detect the new author keywords that appe...
Article
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Mountain protected areas play a fundamental role in the conservation of natural environment and at the same time provide the population with social benefits such as offering space for leisure and recreation. Understanding motivations and behavior of protected area visitors is crucial to effectively manage vulnerable areas. Our objective was to iden...
Article
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A probabilistic discrete model for 2D protein crystal growth is presented. This model takes into account the available space and can describe growing processes of different nature due to the versatility of its parameters which gives the model great flexibility. The accuracy of the simulation is tested against a real protein (SbpA) crystallization e...
Article
Bacillus thuringiensis is a bacterium that produces many insecticidal proteins including cytolytic proteins or Cyt toxins. Although the Cyt toxin shows specific toxicity against Dipteran insect species, the toxin binds directly to the lipid membrane without a specific protein receptor requirement. In this work, we have investigated the interaction...
Article
Full-text available
The aim of this study was to analyze the binding interactions between a common antihypertensive drug (amlodipine besylate—AML) and the widely distributed plant flavonoid quercetin (Q), in the presence of human serum albumin (HSA). Fluorescence analysis was implemented to investigate the effect of ligands on albumin intrinsic fluorescence and to def...
Article
Full-text available
The well-known bacterial S-layer protein SbpA from Lysinibacillus sphaericus CCM2177 induces spontaneous crystal formation via cooperative self-assembly of the protein subunits into an ordered supramolecular structure. Recrystallization occurs in the presence of divalent cations (i.e., Ca2+) and finally leads to producing smooth 2-D crystalline coa...
Article
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This mini review briefly reports on the use of atomic force microscopy (AFM) in the characterization of molecules and (bioengineered) materials related to chemistry, materials science, chemical engineering, and environmental science and biotechnology. Firstly, the operation basics of standard AFM, kelvin probe force microscopy and electrochemistry‐...
Article
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New strategies in regenerative medicine include the implantation of stem cells cultured in bio-resorbable polymeric scaffolds to restore the tissue function and be absorbed by the body after wound healing. This requires the development of appropriate micro-technologies for manufacturing of functional scaffolds with controlled surface properties to...
Article
Mechanical properties of nanoparticles are an important characteristic for drug delivery and therefore, they have gained interest in pharmaceutical research during the last years. Among others, cellular uptake, blood circulation time and accumulation in organs are influenced by the elastic modulus of nanoparticles. Thus, by varying the stiffness of...
Article
Full-text available
Cytolytic protein (Cyt) is a member of insecticidal proteins produced by Bacillus thuringiensis. Cyt protein has activity against insect cells and mammalian cells, which differ in lipid and cholesterol composition. This study presents the lipid binding behavior of Cyt2Aa2 protein on model membranes containing different levels of cholesterol content...
Article
The interplay between protein concentration and (observation) time has been investigated for the adsorption and crystal growth of the bacterial SbpA proteins on hydrophobic fluoride‐functionalized SiO2 surfaces. For this purpose, atomic force microscopy (AFM) has been performed in real‐time for monitoring protein crystal growth at different protein...
Article
The critical role of divalent ions (M²+) in the self-assembly of SbpA S-layer proteins (from Lysinibacillus sphaericus CCM 2177) into crystalline structures has been reported in several studies. Hence, ions such as magnesium, barium, nickel and, most commonly, calcium (Ca²+) have proven to trigger both protein-protein and protein-substrate interact...
Article
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Raman microscopy is a powerful imaging technique for biological materials providing information about chemistry in context with microstructure. A 532 nm laser is often used as excitation source, because high spatial resolution and signal intensity can be achieved. The latter can be controlled by laser power and integration time, whereby high power...
Article
Full-text available
Mucins, the main component of the mucus secretions of goblet and epithelial cells, are known for exhibiting a different behaviour in accordance with their surrounding environment (i.e. among others the environmental pH), which induces a drastic change in their measured mechanical properties. In this work, we have first employed Atomic Force Microsc...
Article
Poly(ε-caprolactone) (PCL) has been blended with Chloramphenicol (CAM), a well-known bacteriostatic antibiotic, in order to obtain new biomaterials with antibacterial properties. The resulting samples have been thoroughly characterized regarding both their physicochemical behavior and antimicrobial efficacy by means of very diverse techniques. Henc...
Article
Beta-lactoglobulin (BLG) and bovine serum albumin (BSA) coacervate formation with sodium alginate (ALG) was investigated by turbidimetric analysis, zeta potential, particle size, viscosity, transmission electron microscopy (TEM) and isothermal titration calorimetric (ITC) measurements as a function of pH (1.0-7.0) and, protein:alginate mixing ratio...
Article
This report focuses on the effect of the surface topography of the substrate on the behavior of human mesenchymal stem cells from bone marrow (MSCs) before and after co-differentiation into adipocytes and osteoblasts. Picosecond pulsed laser ablation technology was applied to generate different microstructures (microgrooves and microcavities) on po...
Article
Fibronectin is an extracellular matrix protein that is involved in cell adhesion, growth, migration, differentiation, and wound healing. Fibronectin coatings are currently used in many laboratories for biomedical and biotechnology purposes. In this study we have investigated the adhesion and mechanical properties of fibronectin coatings. The coatin...
Cover Page
On the cover: The cover image, by Maria Sumarokova et al., is based on the Research Article Adhesion, unfolding forces, and molecular elasticity of fibronectin coatings: An atomic force microscopy study, DOI: 10.1002/jemt.22954.
Article
Full-text available
Mountain protected areas (PAs) aim to preserve vulnerable environments and at the same time encourage numerous outdoor leisure activities. Understanding the way people use natural environments is crucial to balance the needs of visitors and site capacities. This study aims to develop an approach to evaluate the structure and use of designated skiin...
Article
Atomic force microscopy (AFM) is widely used to measure molecular and colloidal inter actions as well as mechanical properties of biomaterials. In this paper the afmToolkit R package is introduced. This package allows the user to automatically batch process AFM force-distance and force-time curves. afmToolkit capabilities range from importing ASCII...
Preprint
Background & study aimThe first Austrian Judo championships took place in 1947. Since then there hasn’t been any scientific literature about technical, tactical or physical preparation about the participants. In addition, there hasn’t been any trauma statistics of Austrian tournaments either. Some scientific studies describe Judo associated injurie...
Article
Full-text available
This review reports on the use of the atomic force microscopy (AFM) in the investigation of cell scaffolds in recent years. It is shown how the technique is able to deliver information about the scaffold surface properties (e.g., topography), as well as about its mechanical behavior (Young's modulus, viscosity, and adhesion). In addition, this shor...
Article
Bacillus thuringiensis is a bacterium known for its bioinsecticide properties. Besides Cry toxins, B. thuringiensis also produces cytolytic proteins (Cyt) that show toxicity against dipteran insect larvae. Two different models have been proposed in order to explain the interaction mechanism of the cytolytic protein Cyt2Aa2 on lipid membranes: pore-...
Article
We report the controlled loss of the anti-fouling activity of the S-layer protein SbpA from Lysinibacillus sphaericus (CCM2177). This protein forms crystal-like films with square lattice (p4) via self-assembly on almost any type of surfaces. Such engineered bioinspired nanometric membranes are known by their excellent preventive performance under b...
Article
Protein-binding interactions are displacement reactions which have been implicated as the causative mechanisms in many drug-drug interactions. Thus, the aim of presented study was to analyse human serum albumin-binding displacement interaction between two ligands, hypoglycaemic drug gliclazide and widely distributed plant flavonoid quercetin. Fluor...
Code
an R package for automatic AFM F-d curve analysis
Article
Full-text available
Optical diffraction measurements are reported for in situ observation of swelling and collapsing of responsive hydrogel nanostructures. The optical signal is enhanced by probing the surface carrying periodic arrays of hydrogel nanostructures by resonantly excited optical waveguide modes. UV-nanoimprint lithography is employed for the preparation of...
Article
Full-text available
The recombinant bacterial surface layer (S-layer) protein rSbpA of Lysinibacillus sphaericus CCM 2177 is an ideal model system to study non-classical nucleation and growth of protein crystals at surfaces since the recrystallization process may be separated into two distinct steps: (i) adsorption of S-layer protein monomers on silicon surfaces is co...
Article
Full-text available
Quartz crystal microbalance with dissipation monitoring (QCM-D) has been employed to study the assembly and recrystallization kinetics of isolated SbpA bacterial surface proteins onto silicon dioxide substrates of different surface wettability. Surface modification by UV/ozone oxidation or by vapor deposition of 1H,1H,2H,2H-perfluorododecyltrichlor...
Article
Cell adhesion forces are typically a mixture of specific and nonspecific cell-substrate and cell–cell interactions. In order to resolve these phenomena, Atomic Force Microscopy appears as a powerful device which can measure cell parameters by means of manipulation of single cells. This method, commonly known as cell-probe force spectroscopy, allows...