José E. Barboza-Corona

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José E. verified their affiliation via an institutional email.

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• DSc (Biotechnology)
• Full professor at University of Guanajuato

The chitin-binding domain (CBD) of chitinases is crucial for substrate-binding, antibacterial, and antifungal activities. Here, we constructed various mutants to investigate the role of the exposed aromatic residues of the CBD of chitinase ChiA74 from Bacillus thuringiensis. One mutant lacked the CBD, three had mutations in surface aromatic residue...

Synthetic biology (SynBio) makes biology easier by leveraging engineering principles and other disciplines to design and construct biological systems with novel or enhanced functions. SynBio has led to the development of more sustainable biotechnological innovations that are in harmony with the environment, aiding the shift from a traditional to a...

Lactococcus lactis is a Gram-positive bacterium used to produce fermented foods and heterologous proteins. Its Nisin-controlled gene expression system stands out for its versatility and safety. However, the lower GC content in its genome may lead to some limitations in protein production. In this study, we explored the importance and effect of codo...

Some Mexican spirits are made with agave cores; however, the leaves are rarely used and transformed into something useful. In this work, we aim to determine if ensiling agave leaves can generate an economic income in two agave-producing communities in Guanajuato. The leaves were collected, transported, and ensiled to achieve it, and the benefit-cos...

En México, el estado de Guanajuato es uno de los principales productores de brócoli y agave, los cuales se usan como alimento o en la elaboración de bebidas. De estas plantas se generan subproductos de donde se pueden extraer compuestos bioactivos. Además, las hojas de agave pueden ser transformadas en textiles, o ensilarlas para generar alimento p...

Simple Summary Aedes, Culex, and Anopheles mosquitoes are the most prolific arthropod vectors of viral and parasitic agents of debilitating and lethal diseases in humans and animals. Despite some success in integrated pest management programs to control vectors, mosquito-borne diseases, such as dengue and dengue hemorrhagic fever, yellow fever, chi...

Abstract The chitinase ChiA74 is synthesized by Bacillus thuringiensis and possesses a modular organization composed of four domains. In the C-terminal of the enzyme is located the chitin-binding domain (CBD), which has not been isolated as a single unit or characterized. Here, we aimed to isolate the ChiA74's CBD as a single unit, determine the b...

Pleurotus fungi, also known as basidiomycetous fungi, have been a part of human culture for thousands of years. They exhibit anticancer, antitumor, antibacterial, and immunomodulatory effects, having biotechnological, medicinal, and aesthetic applications. They are also versatile, highly resistant to illnesses and pests, and do not require special...

This research evaluated the effect of the organic loading rate (OLR, g total solids (TS)/kg.d) at two temperature regimes on hydrogen productivity via consolidated bioprocess (CBP) of raw wheat straw in reactors operated in semicontinuous mode. The OLR from 6.0 to 20.0 g-TS/kg.d and from 4.0 to 30.0 g-TS/kg.d were assessed at 37 and 55 °C, respecti...

The characterization of the Bacillus thuringiensis (Berliner) LBIT-418 strain was based on a previous work which indicated its high insecticidal potential. Therefore, toxicological, molecular, and biochemical characterizations were conducted in this work to identify its unique features and its potential to be developed as a bioinsecticide. This str...

Aims: The objective of this study was to evaluate the inhibitory activity of compounds secreted by bacteria isolated from a hydrogen-producing bioreactor to understand how these microorganisms interact in this community. Methods and results: In vitro inhibitory assays were performed using samples secreted by bacteria subject to different treatme...

Thurincin H is a bacteriocin produced by Bacillus thuringiensis, it is encoded in a group of ten genes, most of which have been characterized experimentally or by homology. However, the activity of the thnI gene encoding a 95 amino acid ORF remains unknown. In this work, the thnI gene was cloned under the regulation of two promoters and transformed...

Lactococcus lactis is a lactic acid bacterium of great importance for the food industry. Due to L. lactis safety status, systems have been designed for the expression of recombinant proteins of biotechnological interest. A peptide with biotechnological importance is the bacteriocin thurincin H, which has many favorable characteristics to be used as...

Thurincin H, a bacteriocin produced by Bacillus thuringiensis, exhibits antibacterial activity against Gram-positive and Gram-negative bacteria. While much is known about its expression and antimicrobial spectrum, its hemolytic property has yet to be established. In this study, thurincin H was produced in a plasmid-free acrystalliferous strain of B...

El objetivo fue analizar la relación entre el perfil de resistencia a antibióticos y la formación de biofilm de aislados de S. aureus provenientes de mastitis bovina. Se analizaron 30 aislados de S. aureus procedentes de casos de mastitis subclínica en granjas lecheras en sistemas de producción semi-intensivo y de traspatio ubicadas en los estados...

The objective was to analyze the relationship between the antibiotic-resistance profile and the biofilm formation of S. aureus isolates from bovine mastitis. Thirty (30) isolates of S. aureus from cases of subclinical mastitis in dairy farms in semi-intensive production and backyard production systems, located in the states of Guanajuato and Michoa...

Aims: The objective of this study was to produce thurincin H, ChiA74 and Cry proteins together using B. thuringiensis subsp. kurstaki HD1 as a heterologous host. Methods and results: pSTAB-ThurH and pSTAB-ChiA74 constructs were designed to produce thurincin H and chitinase respectively, at the sporulation phase. They were transformed into Bt HD1...

The study aimed to identify interspecies interactions within a native microbial community present in a hydrogen-producing bioreactor fed with two wheat straw cultivars. The relationships between the microbial community members were studied building a canonical correspondence analysis and corroborated through in vitro assays. The results showed that...

Cellulases from Streptomyces species can be used as auxiliary agents in the pretreatment of lignocellulosic materials, but little work has been done on this topic. Besides, Streptomyces strains have been identified from Cuatro Cienegas basin, but their potential in the treatment of agricultural wastes has not been demonstrated. In this work a Strep...

La biología sintética permite diseñar y construir sistemas biológicos novedosos o rediseñarlos para que sean predecibles y reproducibles con baseen principios de la ingeniería y la programación, acopladas a un diseñosistemático. Por ejemplo, se pueden generar biosensores basados en células completas o en sus componentes (sistemas libres de células)...

Agaves are a group of succulent plants that thrive in arid or semiarid environments. Indeed, genes associated with their resilience are a potential resource for genetic engineering of other agronomically important crops grown in adverse climates. Agave is mainly used for the production of distilled (spirits) and non‐distilled alcoholic beverages, i...

Thurincin H is a bacteriocin produced by Bacillus thuringiensis with activity against a wide range of bacteria, including Gram positive and Gram negative. Disadvantages of producing thurincin H in B. thuringiensis is the low production level in the native strain probably due to the highly regulated mechanism of biosynthesis. The present study aimed...

The dark fermentation process is a base platform in which lignocellulosic substrates are biologically pretreated producing a gas mixture of H2/CO2, volatile fatty acids as intermediate chemicals, and solids enriched in cellulose. Optimal amounts of nutrients are required by mixed cultures to hydrolyze and ferment the substrate at once requiring opt...

Bacillus cereus s.l., Gram-positive endospore-forming bacilli, persist ubiquitously in different natural habitats and play various ecological roles. Nevertheless, although chitin is one of the most abundant polymer on Earth, the study of the ability of B. cereus s.l. to hydrolyze this polymer were limited to individual B. cereus and B. thuringiensi...

The objective of this study was to determine whether seeds of Brassica oleracea var. italica (i.e. broccoli, an edible plant) produce defensins that inhibit phytopathogenic fungi and pathogenic bacteria of clinical significance. Crude extracts obtained from broccoli seeds were fractioned by molecular exclusion techniques and analyzed by liquid chro...

Streptomyces spp. are Gram-positive bacteria well-known for their ability to produce antibiotics and other metabolites, but few studies on bacteriocins produced by these bacteria have been reported. We tested eight Streptomyces strains against different pathogenic bacteria, and selected S. griseus, S. nigrescens, S. bottroprensis, and S. violaceoru...

The most important bioinsecticide used worldwide is Bacillus thuringiensis and its hallmark is a rich variety of insecticidal Cry protein, many of which have been genetically engineered for expression in transgenic crops. Over the past 20 years, the discovery of other insecticidal proteins and metabolites synthesized by B. thuringiensis, including...

Currently, there is an interest in studying natural inhibitors of dipeptidyl peptidase IV (DPP‐IV) for the treatment of type II diabetes. To look for novel inhibitors, the primary and secondary whey of fresh and Oaxaca cheeses was obtained, and the electrophoretic patterns were determined. Hydrolysis with pepsin and trypsin of primary and secondary...

There is no structural information about any chitinase synthesized by Bacillus thuringiensis, themost successful microbial insect larvicide used worldwide. In this study, we solved the 3D structure of the chitinase ChiA74 at 2.26 Å. The crystal structure shows that ChiA74 is composed of a modular arrangement formed by (i) a catalytic region (CD), (...

The British Standard protocol currently used to determine the nisin concentration in cheese requires the manipulation of a 40 g sample involves two critical points, pH adjustment and heating/ cooling. In this work, we developed a fast micromethod that permits the manipulation of 0.2 g cheese, substitutes the use of 0.02N HCl by 50 mM citric acid an...

Bovine mastitis is a multifactorial disease that produces important losses in livestock and dairy industry. One of the main factors that causes this disease are microorganisms such as Staphylococcus aureus, which has multiresistance to antibiotics and virulence factors such as its ability to internalize into bovine mammary epithelial cells (bMECs),...

The structural gene that encodes thurincin H, a bacteriocin produced by Bacillus thuringiensis, is harboured in a genetic cluster (thnP, E, D, R, A1, A2, A3, B, T, I) that controls its synthesis, modification, secretion and autoimmunity. The specific genes in the cassette that confer immunity in B. thuringiensis to thurincin H are unknown. To ident...

Objectives To develop a recombinant strain of Bacillus thuringiensis that synthesizes two bacteriocins that enhance the antibacterial potency of the strain and that could have applied clinical and industrial value. Results We cloned the thurincin H cluster into the pHT3101 vector by assembling two genetic cassettes harboring genes for the synthesis...

Our objective was to determine whether a recombinant chitinase ChiA74∆sp of Bacillus thuringiensis and its truncated versions (ChiA74∆sp-60, ChiA74∆sp-50) could be produced in B. thuringiensis HD1 with no detrimental effect on the size and insecticidal activity of the native bipyramidal Cry crystal. chiA-p, the promoter used to drive chitinase gene...

Physalis cinerascens fruit. Color analysis through digital images using a computational algorithm.

Staphylococcus aureus is an opportunistic pathogen that may cause severe infections in livestock, and represents the major cause of mastitis in dairy cows. Currently, instead of using antibiotics, new strategies are sought to reduce this clinical health problem. The aim of this study was to determine the efficacy of phage therapy to kill S. aureus...

Biocontrol de fitopatógenos e incremento del vigor en semillas de tomate (Solanum lycopersicum L.) por Bacillus thuringiensis y sus bacteriocinas Biocontrol of phytopathogens and increased vigor in tomato seeds (Solanum lycopersicum L.) by Bacillus thuringiensis and its bacteriocins ID 1 er Autor: Norma Margarita, De La Fuente-Salcido / ORC ID: 000...

Bacillus thuringiensis is a microbial insecticide whose presence in the Fernandez Canyon State Park, a protected natural area in the north of Mexico, has not been reported. The objective of this work was to isolate Bacillus thuringiensis strains isolated from the Fernandez Canyon State Park with the capacity to synthesize antimicrobial peptides (ba...

Biocontrol de fitopatógenos e incremento del vigor en semillas de tomate (Solanum lycopersicum L.) por Bacillus thuringiensis y sus bacteriocinas Biocontrol of phytopathogens and increased vigor in tomato seeds (Solanum lycopersicum L.) by Bacillus thuringiensis and its bacteriocins ID 1 er Autor: Norma Margarita, De La Fuente-Salcido / ORC ID: 000...

The objective of this research was to identify and characterize the encoded peptides present in nut storage proteins of Carya illinoinensis . It was found, through in silico prediction, proteomic analysis, and MS spectrometry, that bioactive peptides were mainly found in albumin and glutelin fractions. Glutelin was the major fraction with ~53% of t...

Aims: The objective of this study was to show whether the edible part of broccoli has antibacterial and antifungal activity against microorganism of importance in human health and vegetable spoilage, and to test if this effect was partially due to antimicrobial peptides. Methods and Results: Crude extracts were obtained from florets and stems of b...

Se evalúa el efecto del contenido de sólidos totales de (10 y 20%) y temperatura (37 y 55°C) sobre la producción de hidrógeno en reactores en lote utilizando paja de trigo como sustrato e inóculo.

In this work, we use the records from 643 goats in order to evaluate the phenotypic relationships between conformation traits and milk production in goats. Correlations were estimated using data on goats which included fifteen conformation traits and first lactation milk yield adjusted for age, calving month and 305 milking days (MY1). We also comp...

ChiA74 has modular structure that includes a secretion signal peptide (sp) sequence, and catalytic (CD), chitin insertion (CID), fibronectin type-III (FnIII) and chitin binding (CBD) domains. We described for the first time the existence of a putative CID in ChiA74. Mature ChiA74 lacking its sp sequence (rChiA74Δsp, ∼70 kDa) and two truncated versi...

In this study, the endochitinase chiA74 gene lacking its secretion signal peptide sequence (chiA74∆sp) was fused in frame with the sequence coding for the C-terminal crystallization domain and transcription terminator of cry1Ac. The chimeric gene was expressed under the strong pcytA-p/STAB-SD promoter system in an acrystalliferous Cry⁻B strain of B...

Thurincin H is a bacteriocin synthesized by some strains of Bacillus thuringiensis. In this study, the thurincin H genetic cassette, which contains ten genes, from a Mexican strain of B. thuringiensis subsp. morrisoni (Btm) was cloned and sequenced. To study the function of the thnR gene component in the cassette, we generated various constructs wi...

Figure S1. Inhibition of rChiA Btt on the growth of C. gloeosporioides using the well diffussion assay. (A) The fungus was placed into the center of fresh potato dextrose agar and different chitinase concentrations were added in each well. Wells (a) without chitinase or added with (b) 18.75 U, (c) 9.38 U, (d) 4.69 U, (e) 3.34 U, and (f) 1.17 U. (B)...

Mastitis caused by microbial infections in dairy goats reduces milk yield, modifies milk composition, and potentially contributes to morbidity in herds and consumers of dairy products. Microorganisms associated with mastitis in dairy goats are commonly controlled with antibiotics, but it is known that continued use of these chemical agents promotes...

Bacillus thuringiensis subsp. tenebrionis DSM-2803 has been studied extensively and spore/crystal mixtures of this strain are used widely in commercial products to control coleopteran pests. The endochitinase chiA Btt gene of B. thuringiensis subsp. tenebrionis DSM-2803 was cloned and expressed in Escherichia coli. The recombinant 6x-histidine tagg...

Currently, there are few reports on the isolation of microorganisms from goat milk and goat cheese that have antibacterial activity. In particular, there are no reports on the isolation of microorganisms with antibacterial activity from these products in central Mexico. Our objective was to isolate bacteria, from goat products, that synthesized ant...

The purpose of this work was to isolate and identify native bacteria from plants collected in the State of Yucata´n, Me´xico with the ability to promote growth of chili pepper (Capsicum annuum L. cv Jalapen˜o). We identified nine bacterial isolates that belong to five species of Bacillus (i.e. Bacillus subtilis, B. flexus, B. cereus, B. megaterium...

ABSTRACT: The efficiency in vitro and in vivo of a bioactive coating formulated with chitosan and mesquite extract was investigated for postharvest biocontrol of plant pathogenic fungi (C. gloeosporoides, F. oxysporum, R. oryzae and R. stolonifer) in fruit and vegetables (strawberry, cherry tomatoes and baby carrots). Separately chitosan and mesqui...

La generación de resistencias a fungicidas de algunos hongos residentes del suelo representa un riesgo biológico en la producción de fresa por lo que su identificación permite mejores medidas de control alternas a pesticidas químicos como algunos péptidos de origen bacteriano. Se amplificaron por PCR las secuencias ITS y EF-1α de 14 hongos aislados...

ABSTRACT: The purpose of this study was to assess the in vitro and in vivo antifungic activity of extract from mesquite (EM) Prosopis glandulosa to evaluate the fungitoxic potential to enrich the formulation of coatings and/or films to prolong the quality and shelf life of fruits and vegetables and protecting them from biological damage. The EM was...

Aims: The objective of this study was to produce stable inclusions of chitinase ChiA74Δsp in Bacillus thuringiensis subsp. israelensis (Bti) and to assay its insecticidal activity against Aedes aegypti larvae. Methods and results: Bti was transformed with chiA74Δsp regulated by its own promoter or by the strong chimeric cytAp/STAB-SD promoter sy...

Unlabelled: The insecticidal activity of Bacillus thuringiensis is owing to the action of Cry and Cyt proteins. In addition to the synthesis of insecticidal proteins, some strains are able to synthesize β-exotoxin, which is highly toxic to humans. In this regard, it is very important to have a simple method to detect β-exotoxin to avoid the commer...

Mexican Tuba (M-Tuba) and Tepache are Mexican fermented beverages prepared mainly with pineapple pulp and coconut palm, respectively. At present, reports on the microbiota and nutritional effects of both beverages are lacking. The purpose of this study was to determine whether M-Tuba and Tepache contain cultivable lactic acid bacteria (LAB) capable...

We would like to express our appreciation to all the authors for their informative contributions and the reviewers for their support and constructive critiques in making this special issue possible.

Thirty-two farms ( n= 535 cows) located in the state of Guanajuato, Mexico, were sampled. Pathogens from bovine subclinical mastitis (SCM) and clinical mastitis (CLM) were identified by 16S rDNA and the sensitivity to both antibiotics and bacteriocins of Bacillus thuringiensis was tested. Forty-six milk samples were selected for their positive Cali...

The articles contained in the present issue include both reviews and basic scientific studies focused on characterizing AMPs from different sources to evaluate their numerous biological activities. This issue comprises the description of effects of naturally occurring AMPs from bacteria, plants, humans, and pigs as well as the effect of synthetic A...

Bacteriocins synthesized by entomopathogenic Bacillus thuringiensis are gaining attention owing to their inhibitory effects against a wide variety of pathogenic bacteria. In the present study, we purified and characterized Tolworthcin 524, a bacteriocin synthesized by B. thuringiensis subsp. tolworthi, and compared it with other bacteriocins synthe...

Metarhizium anisopliae is a widely studied model to understand the virulence factors that participate in pathogencity. Proteases such as subtilisin-like enzymes (Pr1) and trypsin-like enzymes (Pr2) are considered important factors for insect cuticle degradation. In four M. anisopliae strains (798, 6342, 6345, 6347), the presence of pr1 and pr2 gene...

Animal origin foods are widely distributed and consumed around the world due to their high nutrients availability but may also provide a suitable environment for growth of pathogenic and spoilage microorganisms. Nowadays consumers demand high quality food with an extended shelf life without chemical additives. Edible films and coatings (EFC) added...

Bacillus thuringiensis is one of the most important antimicrobial insecticide worldwide. This bacterium synthesizes insecticidal Cry or Cyt proteins that kill insect larvae pest or parasitic and viral disease vectors. This microorganism also produces chitinases and bacteriocins that can be used to synergize the toxic effect of Cry proteins or be us...

The endochitinase ChiA74 is a soluble secreted enzyme produced by Bacillus thuringiensis that synergizes the entomotoxigenecity of Cry proteins that accumulate as intracellular crystalline inclusion during sporulation. The purpose of this study was to produce alkaline-soluble ChiA74[increment]sp inclusions in B. thuringiensis, and to determine its...

A ctualmente la fresa está conside-rada en nuestro país como uno de los productos agrícolas más importan-tes en cuanto al volumen de siembra y el valor de la producción (SAGARPA, 2013), por lo tanto su producción im-pacta directamente en la desarrollo de empleos y en la generación de impor-tantes divisas en nuestro país. La fresa (Fragaria spp. Fam...

Various strains of Bacillus thuringiensis are among the most successful entomopathogenic bacteria used commercially as biopesticides owing to their ability to synthesize insecticidal crystal (Cry) and cytolytic (Cyt) protein toxins during sporulation, and vegetative insecticidal (VIPs) proteins during the vegetative phase of growth. Whereas much is...

The chitinase gene was molecularly characterized in five Bacillus thuringiensis Mexican isolates, MR10, MR11, MR21, MR33, and RN52. The proteins derived from these genes were tested for their chitinase activity using fluorogenic chitin derivatives. In order to verify if chitinase genes were functional, they were cloned, and enzymatic activity of re...

In the present study, we expressed the chiA74 gene of Bacillus thuringiensis in Escherichia coli K12 and demonstrated that the active ChiA74 enzyme was produced at a high level in this strain. The ChiA74 enzymatic activity (in units per milliliter) was approximately 500 % greater in E. coli K12 when compared to that produced in E. coli DH5α. Moreov...

Previously we described a rapid fluorogenic method to measure the activity of five bacteriocins produced by Mexican strains of Bacillus thuringiensis against B. cereus 183. Here we standardize this method to efficiently determine the activity of bacteriocins against both Gram-positive and Gram-negative bacteria. It was determined that the crucial p...

We have shown previously that in the presence of inducer Bacillus cereus 183, significant increases in bacteriocin production and bactericidal activity of B. thuringiensis occur when the latter is cultivated at pH 7.2, 28°C, and 180 rpm. Here we show that this activity can be further improved when B. thuringiensis is induced with B. cereus 183 and...

Although several strains of B. subtilis with antifungal activity have been isolated worldwide, to date there are no published reports regarding the isolation of a native B. subtilis strain from strawberry plants in Mexico. A native bacterium (Bacillus subtilis 21) demonstrated in vitro antagonistic activity against different plant pathogenic fungi....

Bacillus thuringiensis is the most important entomopathogenic microorganism, owing to its insecticidal crystal proteins (Cry) that are nontoxic to the human beings and represent the hallmark of this bacterium. Most of the studies performed with this microorganism are focused to understand the role of Cry proteins in toxicity, mainly because these c...

The cry19A operon of Bacillus thuringiensis subsp. jegathesan encodes two proteins, mosquitocidal Cry19A (ORF1; 75 kDa) and an ORF2 (60 kDa) of unknown function. Expression of the cry19A operon in an acrystalliferous strain of B. thuringiensis (4Q7) yielded one small crystal per cell, whereas no crystals were produced when cry19A or orf2 was expres...

En los últimos años, los alimentos modificados en su composición (�light� o �lite�), se han vuelto muy populares. Sin embargo, a pesar de que la Norma Oficial Mexicana NOM-086-SSA1, 1994 define a cada uno de estos productos, hay un total desconocimiento entre los consumidores, con relación a su clasificación y uso adecuado. En este trabajo se anali...

Las quitinasas sintetizadas por plantas, hongos, insectos y bacterias tienen un gran poten­cial debido a su amplio espectro de aplicaciones. En este trabajo revisamos generalidades sobre las quitinasas y quitosanasas bacterianas y su uso en la producción de quito-oligo­sacáridos. Este tipo de biomoléculas han creado un mercado biotecnológico divers...

In this study, 13% of fresh lettuce (Lactuca sativa) samples collected from markets and supermarkets in two cities of Mexico were contaminated with Salmonella spp. From those samples, amplicons of ∼300 base pairs (bp) were amplified, corresponding to the expected size of the invasion (invA) and internal transcribed spacer regions of the 16S and 23S...

The aim of this work was to utilize fresh cactus as a source of dietary fiber and calcium in pound cake formulations. Five pound cake formulations using fresh cactus (Opuntia amyclaea Tenore) were developed (four with sucralosa, one of them without cactus and one with sugar cane and cactus). Sensory evaluation was performed by 60 untrained judges a...